##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11881754 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17783367674503 34.0 31.0 34.0 30.0 34.0 2 32.188705808923494 34.0 31.0 34.0 30.0 34.0 3 32.29448699240869 34.0 31.0 34.0 30.0 34.0 4 35.69080314236434 37.0 35.0 37.0 35.0 37.0 5 35.69246409242272 37.0 35.0 37.0 35.0 37.0 6 35.764601169153984 37.0 37.0 37.0 35.0 37.0 7 35.7198922818971 37.0 37.0 37.0 35.0 37.0 8 35.70750210785377 37.0 37.0 37.0 35.0 37.0 9 37.44194518755396 39.0 38.0 39.0 35.0 39.0 10-11 37.361868668548425 39.0 38.0 39.0 35.0 39.0 12-13 37.29907869663015 39.0 38.0 39.0 35.0 39.0 14-15 38.70995662761575 41.0 39.0 41.0 35.5 41.0 16-17 38.62044185563849 41.0 39.0 41.0 35.0 41.0 18-19 38.57589144666688 41.0 39.0 41.0 35.0 41.0 20-21 38.50379413679158 40.5 39.0 41.0 34.5 41.0 22-23 38.3877198181346 40.0 38.5 41.0 34.0 41.0 24-25 38.26494497361249 40.0 38.0 41.0 34.0 41.0 26-27 38.09652615262023 40.0 38.0 41.0 34.0 41.0 28-29 37.93214116366994 40.0 38.0 41.0 33.0 41.0 30-31 37.89018548944878 40.0 38.0 41.0 33.5 41.0 32-33 37.896276425181 40.0 38.0 41.0 33.0 41.0 34-35 37.9256958189843 40.0 38.0 41.0 33.5 41.0 36-37 38.059613757362754 40.0 38.0 41.0 34.0 41.0 38-39 37.97477447353312 40.0 38.0 41.0 33.0 41.0 40-41 37.84698845810139 40.0 38.0 41.0 33.0 41.0 42-43 37.725646062020814 40.0 38.0 41.0 33.0 41.0 44-45 37.56178751049718 40.0 38.0 41.0 33.0 41.0 46-47 37.37847968406011 40.0 38.0 41.0 32.0 41.0 48-49 37.18637109470538 40.0 37.0 41.0 32.0 41.0 50-51 36.24664674929308 39.0 36.0 40.0 30.0 40.5 52-53 36.33710864574372 39.0 36.0 40.0 30.5 41.0 54-55 36.60571768275963 40.0 36.0 41.0 31.0 41.0 56-57 36.547232167910565 40.0 36.0 41.0 31.0 41.0 58-59 36.32137006034631 39.0 35.5 41.0 30.5 41.0 60-61 36.024300705097914 39.0 35.0 41.0 30.0 41.0 62-63 35.67766030166926 38.5 35.0 41.0 29.0 41.0 64-65 35.2872374314432 38.0 35.0 40.0 29.0 41.0 66-67 34.888718997212024 37.0 35.0 40.0 28.5 41.0 68-69 34.4567790664577 36.5 34.0 39.5 28.0 41.0 70-71 34.002582657409 36.0 34.0 39.0 27.0 41.0 72-73 33.54562752267047 35.5 34.0 38.5 26.0 40.0 74-75 33.07873273592434 35.0 34.0 37.0 26.0 39.5 76-77 32.59637735304064 35.0 33.0 37.0 25.5 39.0 78-79 32.13561339512668 35.0 33.0 36.0 24.0 39.0 80-81 31.678751302206727 35.0 33.0 36.0 22.5 37.5 82-83 31.264482078992714 35.0 32.0 35.5 20.0 37.0 84-85 30.86615313698634 35.0 32.0 35.0 18.0 36.5 86-87 30.507387924375475 35.0 31.5 35.0 14.5 36.0 88-89 30.115396262201692 34.0 31.0 35.0 7.0 36.0 90-91 29.741821325369976 34.0 31.0 35.0 2.0 35.5 92-93 29.31850920327083 34.0 30.0 35.0 2.0 35.0 94-95 28.85595927166982 34.0 30.0 35.0 2.0 35.0 96-97 28.36600656771719 34.0 29.0 35.0 2.0 35.0 98-99 27.782205598601013 34.0 29.0 35.0 2.0 35.0 100-101 25.715945137393014 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1803612995325352 1101 2 0.23068747689413982 1101 3 0.23238678910744426 1101 4 0.17681997004712713 1101 5 0.1625083986430269 1101 6 0.10459814803743939 1101 7 0.13614886491005507 1101 8 0.16583747165490337 1101 9 0.19331749945840926 1101 10-11 0.22249482457711878 1101 12-13 0.240159089473579 1101 14-15 0.2825833410195244 1101 16-17 0.2797079710686319 1101 18-19 0.27468902725266986 1101 20-21 0.2221992193808191 1101 22-23 0.2962302593238917 1101 24-25 0.23578796316419215 1101 26-27 0.2888817656935885 1101 28-29 0.22960750913227201 1101 30-31 0.25645175325514913 1101 32-33 0.2102129583504606 1101 34-35 0.16864891244262026 1101 36-37 0.2582992835645328 1101 38-39 0.3565821071865969 1101 40-41 0.2998326561946243 1101 42-43 0.3049500811259733 1101 44-45 0.32968505675476933 1101 46-47 0.38946125921327024 1101 48-49 0.38224974018259417 1101 50-51 0.3643225736783151 1101 52-53 0.3861626407925485 1101 54-55 0.5334726265570353 1101 56-57 0.4426551729996575 1101 58-59 0.4586351350838882 1101 60-61 0.45244202606038897 1101 62-63 0.429833450601528 1101 64-65 0.45379877993327256 1101 66-67 0.4057505370092187 1101 68-69 0.3551016896759194 1101 70-71 0.37334399882072233 1101 72-73 0.2610797546597894 1101 74-75 0.2523572761517414 1101 76-77 0.21815380027283737 1101 78-79 0.14607319140594655 1101 80-81 0.1182593660989184 1101 82-83 -0.014022087630944924 1101 84-85 0.10251563686164644 1101 86-87 0.114907631881902 1101 88-89 0.19896778432224949 1101 90-91 0.09695200284961558 1101 92-93 0.12311472205075447 1101 94-95 0.14174020510368024 1101 96-97 0.11512511284250948 1101 98-99 -0.004999378312959379 1101 100-101 0.17534660582732897 1102 1 0.20408210520965042 1102 2 0.22842650548101062 1102 3 0.20969219583011522 1102 4 0.18894195651369472 1102 5 0.18860673992658405 1102 6 0.20619935937327227 1102 7 0.14849379610476632 1102 8 0.2270253974864218 1102 9 0.25750955090530425 1102 10-11 0.2637544418318569 1102 12-13 0.24089358871550814 1102 14-15 0.3155369625851847 1102 16-17 0.31385177419597454 1102 18-19 0.29910853228272316 1102 20-21 0.3266134115908841 1102 22-23 0.31157634302677195 1102 24-25 0.33635505041672076 1102 26-27 0.30803309331086837 1102 28-29 0.3289213435717926 1102 30-31 0.34670303200158514 1102 32-33 0.2911591701053666 1102 34-35 0.32078854046920213 1102 36-37 0.38570155228134695 1102 38-39 0.3840937510008686 1102 40-41 0.3824587003472004 1102 42-43 0.36386457224825364 1102 44-45 0.33975038097770494 1102 46-47 0.30857529885543755 1102 48-49 0.35375962609359135 1102 50-51 0.41043746275558846 1102 52-53 0.3506580472576033 1102 54-55 0.5212953234383733 1102 56-57 0.4200204671436367 1102 58-59 0.4460180841208867 1102 60-61 0.4308636697966577 1102 62-63 0.3630289650900025 1102 64-65 0.30229039697395876 1102 66-67 0.32329481818461403 1102 68-69 0.39649896639246407 1102 70-71 0.36141064757824637 1102 72-73 0.31745190961648717 1102 74-75 0.26940048301629815 1102 76-77 0.20948537285239155 1102 78-79 0.2911177940677092 1102 80-81 0.2544027424474393 1102 82-83 0.2644026180992505 1102 84-85 0.20857254360503674 1102 86-87 0.2678576729989004 1102 88-89 0.20584595293233576 1102 90-91 0.01761283600406216 1102 92-93 0.04667912389782458 1102 94-95 0.04777695601916321 1102 96-97 0.12229422068307017 1102 98-99 0.12218046673574534 1102 100-101 0.34397248118710166 1103 1 0.09268261888554719 1103 2 0.13556639651693558 1103 3 0.07939685938636387 1103 4 0.08526838845080675 1103 5 0.0923319862386549 1103 6 0.11057731648273972 1103 7 0.09030081493497732 1103 8 0.12749605684067689 1103 9 0.1582814135118582 1103 10-11 0.1371792849581297 1103 12-13 0.15197891331848012 1103 14-15 0.11050855570361051 1103 16-17 0.09597143198271141 1103 18-19 0.047529905316906707 1103 20-21 0.1040401449965529 1103 22-23 -0.024680041738882608 1103 24-25 0.04254473945659498 1103 26-27 0.08178105481239584 1103 28-29 0.15747273437951748 1103 30-31 0.04885834343755846 1103 32-33 0.08697539808940036 1103 34-35 0.05870817724197508 1103 36-37 0.03804876629428833 1103 38-39 0.04862706507806536 1103 40-41 0.12068699560482088 1103 42-43 0.07360422292925506 1103 44-45 0.11188421422958328 1103 46-47 0.041883866884454335 1103 48-49 0.07952520325731172 1103 50-51 0.022580967948925945 1103 52-53 0.04076652548589976 1103 54-55 0.11588127427347672 1103 56-57 0.042438550033999434 1103 58-59 0.14243738706080222 1103 60-61 0.1564954863043866 1103 62-63 0.22955153752584323 1103 64-65 0.20198670889116244 1103 66-67 0.13839696946978108 1103 68-69 0.11647779353201315 1103 70-71 0.03486207979882039 1103 72-73 0.01094523757611654 1103 74-75 0.073204955625215 1103 76-77 0.03053515452301525 1103 78-79 0.012871752095875877 1103 80-81 -0.04077626324419015 1103 82-83 -0.07314325511012854 1103 84-85 -0.09536681239122302 1103 86-87 -0.2135336256123459 1103 88-89 -0.1884971055369462 1103 90-91 -0.26884827196710503 1103 92-93 -0.2236278142513548 1103 94-95 -0.21115834028431024 1103 96-97 -0.3396544168850397 1103 98-99 -0.3842291768610089 1103 100-101 -0.12666231748774948 1104 1 0.16814301843914592 1104 2 0.15964933317252417 1104 3 0.19254232499979196 1104 4 0.17949339814175858 1104 5 0.20822176328651665 1104 6 0.21342063049119275 1104 7 0.2045145855851942 1104 8 0.16277189610425324 1104 9 0.22578551313081618 1104 10-11 0.2676594999307085 1104 12-13 0.257857105289645 1104 14-15 0.2411995674907388 1104 16-17 0.21010232248358562 1104 18-19 0.3688993044105544 1104 20-21 0.36099916150791955 1104 22-23 0.28393278043583337 1104 24-25 0.2986743064671131 1104 26-27 0.41370759643177735 1104 28-29 0.3626380728253693 1104 30-31 0.405499110543559 1104 32-33 0.3143222898116349 1104 34-35 0.3429634372154311 1104 36-37 0.31755941312104596 1104 38-39 0.2108380975081232 1104 40-41 0.20855764451037828 1104 42-43 0.31387954313888855 1104 44-45 0.33454850711545703 1104 46-47 0.25949992274580325 1104 48-49 0.30932891792098616 1104 50-51 0.21186750011258226 1104 52-53 0.22817769176836578 1104 54-55 0.40767059254504545 1104 56-57 0.28223402074723225 1104 58-59 0.26306704680117576 1104 60-61 0.33924734961733094 1104 62-63 0.3235468609858785 1104 64-65 0.3620025837770058 1104 66-67 0.31854630537232964 1104 68-69 0.29863598484480747 1104 70-71 0.18869904190891873 1104 72-73 0.1811288997278595 1104 74-75 0.18556798467629676 1104 76-77 0.16912809842202847 1104 78-79 0.12537445914688305 1104 80-81 0.13600635009457562 1104 82-83 0.18170555763011365 1104 84-85 0.013524128089162701 1104 86-87 0.06468683843613476 1104 88-89 0.15185038973940124 1104 90-91 0.13372711689242678 1104 92-93 0.12216825703515966 1104 94-95 0.07421029364217091 1104 96-97 0.09502795774075068 1104 98-99 0.07529410870251141 1104 100-101 0.2676812062807059 1105 1 -0.20154120325062763 1105 2 -0.04581944429590834 1105 3 -0.10375826064466054 1105 4 -0.020563029423342982 1105 5 0.014476803474792632 1105 6 0.045453528213919014 1105 7 0.07221750648986358 1105 8 0.03817834392986441 1105 9 0.05433451733950534 1105 10-11 0.03558272553694053 1105 12-13 0.03517070231931285 1105 14-15 0.061870962120572415 1105 16-17 0.05978318307935382 1105 18-19 0.08496814399564556 1105 20-21 -0.0239392622389758 1105 22-23 0.09683920988352668 1105 24-25 0.11299749150609983 1105 26-27 0.17377062772605711 1105 28-29 0.23143875764056787 1105 30-31 0.21159175293038857 1105 32-33 0.13276048013443642 1105 34-35 0.13190562277198836 1105 36-37 0.1400752227878499 1105 38-39 0.15762367540715871 1105 40-41 0.06962939536801116 1105 42-43 0.02444770328703072 1105 44-45 0.008632057661799308 1105 46-47 -0.03144693330879278 1105 48-49 0.060577611810977317 1105 50-51 -0.03733023192469176 1105 52-53 0.07053719166098915 1105 54-55 0.2995189321255438 1105 56-57 0.2359027422263651 1105 58-59 0.20757460627320512 1105 60-61 0.187991611908231 1105 62-63 0.2510224434514683 1105 64-65 0.2613803151121985 1105 66-67 0.3109881215431969 1105 68-69 0.3010864266661031 1105 70-71 0.28727462640063095 1105 72-73 0.2522408603539432 1105 74-75 0.12454200499070112 1105 76-77 0.0814875302638427 1105 78-79 0.1194661259427221 1105 80-81 0.17931625308534294 1105 82-83 0.2401375796929166 1105 84-85 0.2238128073339425 1105 86-87 0.16557916800767103 1105 88-89 0.24754105738129795 1105 90-91 0.1434004977798402 1105 92-93 0.09379243024055128 1105 94-95 0.06078917532173378 1105 96-97 -0.0857277718680578 1105 98-99 -0.17009417109660419 1105 100-101 0.03012045010288844 1106 1 -0.18999834624806056 1106 2 -0.0701228036887187 1106 3 -0.09984177797789329 1106 4 0.0698493515960763 1106 5 0.046666986934567944 1106 6 0.0992640663858424 1106 7 0.07237195453353706 1106 8 0.04750190306786095 1106 9 -0.1012307777052186 1106 10-11 -0.09001113291025575 1106 12-13 0.0767973097221244 1106 14-15 0.051311567543514514 1106 16-17 -0.09272123128621956 1106 18-19 0.13762616664946137 1106 20-21 0.11817076530863346 1106 22-23 0.15739287961619652 1106 24-25 0.15444272847323504 1106 26-27 0.17838188944912048 1106 28-29 0.21563101013499875 1106 30-31 0.16696575391784307 1106 32-33 0.2275163496971473 1106 34-35 -0.007765026818468357 1106 36-37 0.1303056656299546 1106 38-39 0.12234685365329057 1106 40-41 0.17370845440893135 1106 42-43 0.24864204652141808 1106 44-45 0.2017613711555981 1106 46-47 0.17730310773739433 1106 48-49 0.14021886689215535 1106 50-51 0.03732500313164877 1106 52-53 0.15151760557895244 1106 54-55 0.32115640975798243 1106 56-57 0.26549322697700717 1106 58-59 0.2650507048386217 1106 60-61 0.3192534568888803 1106 62-63 0.3191668537114509 1106 64-65 0.2338849922849633 1106 66-67 0.3094437875754181 1106 68-69 0.32715100570074185 1106 70-71 0.3231035455078839 1106 72-73 0.30988389169922215 1106 74-75 0.35805333718347043 1106 76-77 0.19728555083472799 1106 78-79 0.13569675029475547 1106 80-81 0.33156333541644045 1106 82-83 0.5078661770957176 1106 84-85 0.38506149605303364 1106 86-87 0.4218062754304448 1106 88-89 0.39122746072799686 1106 90-91 0.20245996080673123 1106 92-93 0.27150573098188957 1106 94-95 0.37297626703075437 1106 96-97 0.3931178051693358 1106 98-99 0.4560085275342196 1106 100-101 0.6418809906866656 1107 1 0.028940291143435104 1107 2 0.09550848805054812 1107 3 0.08239729491388204 1107 4 0.12028244135229471 1107 5 0.10304808755418549 1107 6 0.09472701213081791 1107 7 0.10381107976798631 1107 8 0.06402657873838535 1107 9 0.07123261111594559 1107 10-11 0.08605965367624435 1107 12-13 0.17845264695360896 1107 14-15 0.21971369247263084 1107 16-17 0.1778201710843561 1107 18-19 0.20589617222808698 1107 20-21 0.2210963308047056 1107 22-23 0.25754589012375817 1107 24-25 0.24185672463017482 1107 26-27 0.2307083837981807 1107 28-29 0.2842663813892301 1107 30-31 0.308844373999527 1107 32-33 0.2930915108001102 1107 34-35 0.3291704240976472 1107 36-37 0.28128755001232264 1107 38-39 0.30381169108105865 1107 40-41 0.23683177422040558 1107 42-43 0.15200816290123242 1107 44-45 0.23671135864385917 1107 46-47 0.18652012553619812 1107 48-49 0.22500172512384609 1107 50-51 0.17402100924747543 1107 52-53 0.24799446116137602 1107 54-55 0.400782251430563 1107 56-57 0.35767086609259735 1107 58-59 0.34006430704803137 1107 60-61 0.3631853792656017 1107 62-63 0.3210655384868417 1107 64-65 0.32173804710014764 1107 66-67 0.27929222337872517 1107 68-69 0.31594978712644206 1107 70-71 0.2668006145474138 1107 72-73 0.2793388391154892 1107 74-75 0.2839972846020373 1107 76-77 0.30970499935408924 1107 78-79 0.3428890476562714 1107 80-81 0.3967608486800316 1107 82-83 0.41719545616713916 1107 84-85 0.35449635581827366 1107 86-87 0.32889457615291207 1107 88-89 0.39111841347954623 1107 90-91 0.4250312490105763 1107 92-93 0.39641441825963497 1107 94-95 0.39015889936245784 1107 96-97 0.5079818634258189 1107 98-99 0.46027629247885926 1107 100-101 0.5639025659717376 1108 1 0.2550172958571224 1108 2 0.2586532158798036 1108 3 0.2486332238145721 1108 4 0.18241154636530865 1108 5 0.21467422017755666 1108 6 0.2522976415127971 1108 7 0.24507477291290058 1108 8 0.2370837053051673 1108 9 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9 -0.6828137848396949 2316 10-11 -0.8248205942822224 2316 12-13 -0.987884842276209 2316 14-15 -1.2072089492490576 2316 16-17 -1.3226692346717215 2316 18-19 -1.248234755516613 2316 20-21 -1.0597691800945483 2316 22-23 -1.1381348837424525 2316 24-25 -1.0937908088228099 2316 26-27 -1.1156768398118118 2316 28-29 -1.1013495991250224 2316 30-31 -0.9987287945891978 2316 32-33 -0.7837978229221676 2316 34-35 -0.8860463825814335 2316 36-37 -1.1667015210831124 2316 38-39 -1.3062754329653217 2316 40-41 -1.4088398864114708 2316 42-43 -1.5288005374469051 2316 44-45 -1.5396638022548785 2316 46-47 -1.4802029210921646 2316 48-49 -1.8170859980413852 2316 50-51 -2.1867075513236998 2316 52-53 -2.0754222233829296 2316 54-55 -2.0073237633913052 2316 56-57 -2.132593588848785 2316 58-59 -2.132520882071198 2316 60-61 -2.0773894052870716 2316 62-63 -2.2627695241808112 2316 64-65 -1.9132359627404085 2316 66-67 -1.9127957614104503 2316 68-69 -2.0171431464978156 2316 70-71 -1.7801910697352596 2316 72-73 -1.772402388197353 2316 74-75 -1.8607217432644028 2316 76-77 -2.073499287964818 2316 78-79 -1.9113557682651177 2316 80-81 -2.0321151973797384 2316 82-83 -1.892364141333129 2316 84-85 -1.4622176375312463 2316 86-87 -1.4380046142517706 2316 88-89 -2.1248989951962542 2316 90-91 -1.7664972802093324 2316 92-93 -1.4692069026535393 2316 94-95 -1.2805806199820147 2316 96-97 -1.3155957307500792 2316 98-99 -1.1267699752810465 2316 100-101 -1.1603796741659096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102617.0 3 25269.0 4 19703.0 5 15962.0 6 14317.0 7 14135.0 8 15419.0 9 17512.0 10 19803.0 11 23284.0 12 25793.0 13 28407.0 14 31401.0 15 35940.0 16 42508.0 17 51698.0 18 48686.0 19 41839.0 20 43797.0 21 47262.0 22 50637.0 23 57834.0 24 67440.0 25 77566.0 26 91012.0 27 107409.0 28 127564.0 29 152634.0 30 185142.0 31 228707.0 32 290971.0 33 383688.0 34 539527.0 35 826356.0 36 1483187.0 37 2872188.0 38 3153724.0 39 520744.0 40 72.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.53097999963802 18.713119373753845 14.145837603812094 24.610063022796044 2 15.236687399333526 20.379040982047638 39.48742489400106 24.896846724617777 3 18.32233182064112 25.00458589282556 29.591539429712764 27.081542856820555 4 13.388362044618024 17.162068458392707 36.954221188952516 32.49534830803675 5 14.831059969092733 36.58014293053914 33.994745057135674 14.594052043232455 6 32.02262078979145 35.49872615368452 18.358043078431624 14.120609978092402 7 28.647749372212377 31.51116304008067 21.98623425837633 17.854853329330624 8 26.60315336447676 34.18132990802799 20.682377470848927 18.533139256646326 9 27.079641145433282 16.188190543666284 19.866864039821248 36.86530427107918 10-11 25.33672592562535 25.5913808130688 27.86280071211437 21.20909254919148 12-13 26.40126731679721 22.812199039620698 27.72631378676347 23.060219856818627 14-15 23.433935978751943 24.232792414732486 25.061629646081386 27.271641960434188 16-17 23.57518862515187 27.143430893857147 25.840294424475424 23.441086056515555 18-19 23.846869799115673 26.353507649420465 26.924858709396794 22.874763842067072 20-21 24.430519295948443 25.763691313320724 26.56960598492319 23.236183405807644 22-23 24.306503267951843 25.73605117040703 26.45719060548887 23.500254956152258 24-25 24.15318215369388 25.872402051972408 26.400185463920124 23.574230330413588 26-27 23.849205672728957 26.179027487080408 26.231705533299447 23.740061306891185 28-29 23.82450656646498 26.124163625512164 26.247894791761855 23.803435016261 30-31 23.951538748901648 25.80993280963079 26.28056635640887 23.957962085058693 32-33 23.73394126200064 25.974448305155384 26.396296692314625 23.895313740529353 34-35 24.095748185303723 25.97132479932306 26.198322596533842 23.73460441883937 36-37 24.002896724099532 25.725289143080936 26.18934336725066 24.08247076556887 38-39 23.8597062590276 25.73628111071918 26.453720504194965 23.950292126058255 40-41 24.010256058004583 25.415263948413134 26.694400735108122 23.88007925847416 42-43 23.707171894522382 25.57789886982857 26.583901491595636 24.13102774405341 44-45 23.545829016432737 25.68816919851177 26.521900812619048 24.244100972436446 46-47 23.701685513256095 25.612650766577616 26.503250760474877 24.182412959691412 48-49 23.605287762793594 25.731894056004297 26.381741635119177 24.28107654608293 50-51 23.2909028165612 25.859259948586388 26.529277502718013 24.3205597321344 52-53 23.478168228786867 25.718158012020698 26.4390983585994 24.364575400593036 54-55 23.314828335460206 25.53767154394836 26.61268211095709 24.53481800963435 56-57 23.284134498562025 25.75926685667358 26.561280217768413 24.39531842699598 58-59 23.508976934121222 25.499084167562252 26.369349116874208 24.622589781442315 60-61 23.36127076419581 25.30035389825566 26.687351207800592 24.651024129747935 62-63 23.389776461104447 25.322924404348612 26.6480420732352 24.63925706131174 64-65 23.329157348215077 25.328533160148854 26.441907052378134 24.900402439257935 66-67 23.30254136927035 25.59735269667594 26.200632590742728 24.899473343310973 68-69 23.26274766111486 25.281669869137087 26.5038320840605 24.95175038568755 70-71 23.27103386002614 25.180022016278574 26.40315889396615 25.145785229729135 72-73 23.228190020667963 25.2186779255194 26.347468942704594 25.205663111108045 74-75 23.087279229887166 25.318040300595907 26.470528063419472 25.124152406097455 76-77 23.223018150585133 25.080605323180343 26.56682240862085 25.12955411761368 78-79 23.127442374504707 25.04568106906624 26.63767632303362 25.189200233395432 80-81 23.151048217076347 25.108699971075076 26.712644334199737 25.027607477648843 82-83 23.136000045791985 25.019561552696747 26.769955929845164 25.074482471666105 84-85 23.166516668336435 24.911697428251266 26.776192107870827 25.145593795541473 86-87 23.26590032695756 24.85835844584526 26.788856362380482 25.0868848648167 88-89 23.300448660784287 24.624638858271222 26.812719429051423 25.262193051893068 90-91 23.434381945874968 24.6272362018614 26.899091608038216 25.039290244225416 92-93 23.555772168873684 24.501180687123657 26.87499097502979 25.06805616897287 94-95 23.507765081072048 24.343226791508997 26.881756565542226 25.26725156187673 96-97 23.755754359143445 24.178952376828537 26.729921724035737 25.33537153999228 98-99 23.679221998643932 24.014887021046516 26.78341072198532 25.522480258324237 100-101 23.666966244057587 23.892310491329383 27.0791246500251 25.361598614587933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 524.0 1 407.5 2 343.0 3 385.5 4 356.0 5 377.0 6 519.5 7 965.0 8 1749.5 9 2280.0 10 2250.0 11 2210.0 12 2378.0 13 2623.5 14 2923.0 15 3184.0 16 3422.5 17 3762.5 18 4137.5 19 4576.0 20 5196.0 21 6044.0 22 7353.0 23 8946.0 24 10865.0 25 14078.5 26 18525.0 27 24697.5 28 32316.5 29 42169.5 30 56470.5 31 75137.5 32 96372.0 33 119734.5 34 148455.5 35 177505.5 36 206658.5 37 234946.0 38 258313.0 39 275361.0 40 293601.0 41 325020.5 42 357681.5 43 389958.0 44 422725.0 45 446896.0 46 467779.0 47 492306.0 48 520959.5 49 539908.5 50 547609.0 51 547949.5 52 537940.0 53 529444.0 54 516326.5 55 484157.0 56 444566.0 57 400202.5 58 353794.0 59 307311.0 60 254740.5 61 202894.0 62 159644.0 63 123804.0 64 93433.0 65 70380.0 66 52429.0 67 39488.5 68 28433.5 69 19839.0 70 14399.5 71 10266.5 72 7421.5 73 5375.5 74 3973.0 75 3029.0 76 2207.5 77 1458.0 78 925.5 79 634.5 80 468.0 81 377.5 82 286.0 83 221.5 84 189.5 85 167.5 86 143.5 87 116.5 88 97.5 89 87.5 90 74.0 91 68.0 92 64.0 93 51.5 94 46.0 95 38.0 96 29.5 97 22.0 98 18.0 99 12.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022883826748138363 2 0.03401013015418431 3 0.024684907632324317 4 0.023085817127673238 5 0.04154268805767229 6 0.02331305630464997 7 0.026384993326742837 8 0.039236631224649154 9 0.02583793604883589 10-11 0.027984083831393915 12-13 0.02620825174464982 14-15 0.03493171126081217 16-17 0.042283319449300166 18-19 0.0434952617265094 20-21 0.03738505274557948 22-23 0.043781414764183804 24-25 0.019816097858952476 26-27 0.02517725918244057 28-29 0.026751100889649798 30-31 0.026860512345231184 32-33 0.03588695743069584 34-35 0.04011613100220725 36-37 0.029528468608254303 38-39 0.01570054387592943 40-41 0.0180192251076735 42-43 0.019875011719650146 44-45 0.021642427540580288 46-47 0.015498553496394554 48-49 0.024983685068719653 50-51 0.018637820644999047 52-53 0.02726870123720791 54-55 0.023573960544882512 56-57 0.02282912102034767 58-59 0.02247563785616164 60-61 0.01978664092860364 62-63 0.022370434533487228 64-65 0.019538361087092025 66-67 0.018461079062906033 68-69 0.022248398679184905 70-71 0.03517157483650983 72-73 0.02510572092302197 74-75 0.033782890977207575 76-77 0.04256105622116062 78-79 0.02694046687046374 80-81 0.022942740608836033 82-83 0.016373845141045673 84-85 0.032352125788835555 86-87 0.020060169567557115 88-89 0.03052158797430076 90-91 0.023994773835580168 92-93 0.04194246068383506 94-95 0.07455128257999619 96-97 0.09416118192650681 98-99 0.07118898438732194 100-101 0.07973570232139127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1881754E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.26934534533019 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51142110984763 34.93864020848196 2 12.434153816520617 11.506403140270956 3 4.196204224614776 5.8246686722470375 4 2.07239639833236 3.8355369858743367 5 1.2843671750884897 2.9713414187187746 6 0.8246522895401868 2.2893672944731276 7 0.5780770684513934 1.8723073261475522 8 0.44800805893842544 1.6583231677210437 9 0.33073699147685603 1.3772685669406362 >10 2.061730959419585 17.755866799754138 >50 0.16065740050211302 5.12294476277397 >100 0.0905208880015542 7.760346543297008 >500 0.005268070980640703 1.6529382327717546 >1k 0.0017508354166276482 1.2494309367567842 >5k 5.471286875415282E-5 0.1846159437709563 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.416265813953058E-6 1.6832531627906117E-5 2 0.0 0.0 0.0 8.416265813953058E-6 1.6832531627906117E-5 3 0.0 0.0 0.0 1.6832531627906117E-5 1.6832531627906117E-5 4 0.0 0.0 0.0 4.208132906976529E-5 1.6832531627906117E-5 5 0.0 0.0 0.0 5.049759488371834E-5 1.6832531627906117E-5 6 0.0 0.0 0.0 5.049759488371834E-5 2.524879744185917E-5 7 0.0 0.0 0.0 5.049759488371834E-5 2.524879744185917E-5 8 0.0 0.0 0.0 5.89138606976714E-5 4.208132906976529E-5 9 0.0 0.0 0.0 1.4307651883720197E-4 4.208132906976529E-5 10-11 8.416265813953058E-6 0.0 0.0 2.6090424023254475E-4 4.208132906976529E-5 12-13 2.94569303488357E-5 0.0 0.0 3.2823436674416927E-4 5.89138606976714E-5 14-15 3.3665063255812233E-5 8.416265813953058E-6 0.0 3.9977262616277024E-4 6.733012651162447E-5 16-17 4.208132906976529E-5 8.416265813953058E-6 0.0 5.260166133720661E-4 7.574639232557752E-5 18-19 5.049759488371835E-5 8.416265813953058E-6 0.0 7.23798859999963E-4 8.416265813953058E-5 20-21 5.89138606976714E-5 8.416265813953058E-6 0.0 9.299973724418129E-4 1.009951897674367E-4 22-23 5.89138606976714E-5 8.416265813953058E-6 0.0 0.0013802675934883016 1.2203585430231934E-4 24-25 7.574639232557752E-5 8.416265813953058E-6 0.0 0.002541712275813824 1.2624398720929587E-4 26-27 7.574639232557752E-5 8.416265813953058E-6 0.0 0.004830936577209056 1.2624398720929587E-4 28-29 1.0520332267441322E-4 1.2624398720929588E-5 0.0 0.019710894536278063 1.2624398720929587E-4 30-31 1.0941145558138975E-4 1.6832531627906117E-5 0.0 0.07694150207115885 1.2624398720929587E-4 32-33 1.0941145558138975E-4 1.6832531627906117E-5 0.0 0.20223024311057103 1.2624398720929587E-4 34-35 1.2203585430231934E-4 3.3665063255812233E-5 0.0 0.35331483886974935 1.2624398720929587E-4 36-37 1.3466025302324893E-4 3.7873196162788766E-5 0.0 0.592193711467179 1.2624398720929587E-4 38-39 1.4307651883720197E-4 5.049759488371835E-5 0.0 0.9817700315963451 1.2624398720929587E-4 40-41 1.4307651883720197E-4 5.89138606976714E-5 0.0 1.4238259772084154 1.2624398720929587E-4 42-43 1.6832531627906114E-4 5.89138606976714E-5 0.0 1.8526725936254866 1.2624398720929587E-4 44-45 1.8515784790696727E-4 5.89138606976714E-5 0.0 2.311009805454649 1.3886838593022544E-4 46-47 2.2303104406975604E-4 5.89138606976714E-5 0.0 2.826152603395088 1.599090504651081E-4 48-49 2.4407170860463867E-4 5.89138606976714E-5 0.0 3.3554852254978513 1.8094971499999074E-4 50-51 2.4407170860463867E-4 5.89138606976714E-5 0.0 3.8985405690102657 1.9357411372092034E-4 52-53 2.4407170860463867E-4 6.312199360464793E-5 0.0 4.4119117429968675 2.2303104406975604E-4 54-55 2.4407170860463867E-4 6.733012651162447E-5 0.0 4.92434871147812 2.356554427906856E-4 56-57 2.609042402325448E-4 6.733012651162447E-5 0.0 5.481446594501115 2.356554427906856E-4 58-59 2.609042402325448E-4 1.0099518976743669E-4 0.0 6.059265323958062 2.4407170860463867E-4 60-61 2.651123731395213E-4 1.0520332267441322E-4 0.0 6.653441066024428 2.5248797441859174E-4 62-63 2.7773677186045093E-4 1.0941145558138975E-4 0.0 7.229576542318584 2.7773677186045093E-4 64-65 2.8615303767440394E-4 1.0941145558138975E-4 0.0 7.785062710438206 2.8615303767440394E-4 66-67 2.8615303767440394E-4 1.178277213953428E-4 0.0 8.37333865016899 2.8615303767440394E-4 68-69 2.94569303488357E-4 1.178277213953428E-4 0.0 9.000072716536632 2.8615303767440394E-4 70-71 3.156099680232397E-4 1.178277213953428E-4 0.0 9.644321032063111 2.9877743639533357E-4 72-73 3.450668983720754E-4 1.178277213953428E-4 0.0 10.268925783179824 3.156099680232397E-4 74-75 3.7031569581393454E-4 1.2624398720929587E-4 0.0 10.872919099318164 3.2823436674416927E-4 76-77 3.7031569581393454E-4 1.2624398720929587E-4 0.0 11.493416712717668 3.2823436674416927E-4 78-79 3.7031569581393454E-4 1.2624398720929587E-4 0.0 12.13853611175589 3.366506325581223E-4 80-81 4.123970248836998E-4 1.304521201162724E-4 0.0 12.80208713292667 3.534831641860284E-4 82-83 4.208132906976529E-4 1.3466025302324893E-4 0.0 13.465760189951753 3.7031569581393454E-4 84-85 4.250214236046294E-4 1.3466025302324893E-4 0.0 14.109419366871254 3.7031569581393454E-4 86-87 4.2922955651160594E-4 1.3466025302324893E-4 0.0 14.743324091712385 3.745238287209111E-4 88-89 4.2922955651160594E-4 1.3466025302324893E-4 0.0 15.39813902896828 3.829400945348641E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10240 0.0 52.597225 1 GTATCAA 19660 0.0 45.36927 1 ATCAACG 23560 0.0 36.77125 3 TCAACGC 23820 0.0 36.429398 4 CAACGCA 24435 0.0 35.610752 5 TATCAAC 24785 0.0 35.434254 2 AACGCAG 25430 0.0 34.403748 6 ACGCAGA 29545 0.0 29.548347 7 CGCAGAG 30895 0.0 28.118706 8 GTACATG 38490 0.0 25.641731 1 TACATGG 39795 0.0 24.372639 2 ACATGGG 40195 0.0 23.479277 3 GCAGAGT 36785 0.0 23.349325 9 CATGGGG 31480 0.0 20.89634 4 AGAGTAC 32605 0.0 19.881693 10-11 CAGAGTA 34280 0.0 19.575445 10-11 GAGTACT 13985 0.0 17.428171 12-13 TCGCCGT 6965 0.0 17.165092 94-95 ATGGGGA 18560 0.0 16.864576 5 TAGGACG 6365 0.0 16.49108 4 >>END_MODULE