FastQCFastQC Report
Fri 27 May 2016
SRR940192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881754
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109200.052.2235451
GTATCAA207800.046.3570671
TCAACGC256150.036.993544
ATCAACG256950.036.952463
CAACGCA265300.035.735575
TATCAAC271650.035.1464962
AACGCAG275600.034.4172556
ACGCAGA320700.029.5324787
CGCAGAG334250.028.3068568
GTACATG389600.023.5177521
GCAGAGT401450.023.2135359
TACATGG402650.022.3669262
ACATGGG403000.021.6866263
AGAGTAC356550.020.42848610-11
CATGGGG315450.019.7702264
CAGAGTA369850.019.74523710-11
GAGTACT161500.018.6469912-13
GTACTTT175000.017.439714-15
ATGGGGA192500.016.704575
AGTACTT181600.016.30844712-13