FastQCFastQC Report
Fri 27 May 2016
SRR940191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11940500
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90200.046.2500421
TATAACG9150.043.100752
GTATCAA160350.041.8694571
TATCACG10850.038.9751742
TAACGCA11500.036.769614
ATAACGC11200.036.483113
TCAACGC193150.035.298324
ATCAACG192900.035.2467273
CAACGCA204050.033.5270585
ATCACGC13850.032.590073
TATCAAC204500.032.2031022
AACGCAG216800.032.026296
TACCTGG105750.029.6994842
GTACATG309800.027.8214651
ACGCAGA252950.027.4498717
CGCAGAG263300.026.3161878
GTATAAC23850.026.297211
ACATGGG334700.025.4670223
TACATGG342450.024.9054892
GTACCTG142200.022.6544881
Produced by FastQC (version 0.11.5)