##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11940500 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845376575520287 33.0 31.0 34.0 30.0 34.0 2 30.816151919936352 33.0 31.0 34.0 26.0 34.0 3 31.660298312465976 34.0 31.0 34.0 28.0 34.0 4 35.416508856413046 37.0 35.0 37.0 33.0 37.0 5 35.58436296637494 37.0 35.0 37.0 35.0 37.0 6 35.733415016121604 37.0 37.0 37.0 35.0 37.0 7 35.71029152883045 37.0 37.0 37.0 35.0 37.0 8 35.70353996901302 37.0 37.0 37.0 35.0 37.0 9 37.452995938193546 39.0 38.0 39.0 35.0 39.0 10-11 37.38455006071773 39.0 38.0 39.0 35.0 39.0 12-13 37.30859779741217 39.0 38.0 39.0 35.0 39.0 14-15 38.72693295925632 41.0 39.0 41.0 35.5 41.0 16-17 38.64451940873498 41.0 39.0 41.0 35.0 41.0 18-19 38.575050374774925 41.0 39.0 41.0 35.0 41.0 20-21 38.50082069427578 40.5 39.0 41.0 34.5 41.0 22-23 38.387884008207365 40.0 38.5 41.0 34.0 41.0 24-25 38.2769899082953 40.0 38.0 41.0 34.0 41.0 26-27 38.08326581801432 40.0 38.0 41.0 34.0 41.0 28-29 37.90840195134207 40.0 38.0 41.0 33.0 41.0 30-31 37.88312373853691 40.0 38.0 41.0 33.5 41.0 32-33 37.90460512541351 40.0 38.0 41.0 33.0 41.0 34-35 37.90232314392195 40.0 38.0 41.0 33.5 41.0 36-37 37.99072329466941 40.0 38.0 41.0 33.5 41.0 38-39 37.91083107910054 40.0 38.0 41.0 33.0 41.0 40-41 37.78264679033541 40.0 38.0 41.0 33.0 41.0 42-43 37.513707047443575 40.0 38.0 41.0 32.5 41.0 44-45 37.46441133118378 40.0 38.0 41.0 32.5 41.0 46-47 37.29936292450065 40.0 38.0 41.0 32.0 41.0 48-49 37.11159051128512 40.0 37.0 41.0 31.5 41.0 50-51 36.175619739541894 39.0 36.0 40.0 30.0 40.5 52-53 36.24154017838449 39.0 36.0 40.0 30.5 41.0 54-55 36.58992328629455 40.0 36.0 41.0 31.0 41.0 56-57 36.41224341526737 39.5 36.0 41.0 30.5 41.0 58-59 36.14583610401574 39.0 35.0 41.0 30.0 41.0 60-61 35.87270620995771 39.0 35.0 41.0 30.0 41.0 62-63 35.525189941794736 38.5 35.0 40.5 29.0 41.0 64-65 35.133994765713325 37.5 35.0 40.0 29.0 41.0 66-67 34.749069511326994 37.0 35.0 40.0 28.0 41.0 68-69 34.344954231397345 36.5 34.5 39.0 28.0 41.0 70-71 33.90545948662116 36.0 34.0 39.0 27.5 40.5 72-73 33.44122942925338 35.0 34.0 38.5 26.0 40.0 74-75 32.9730537247184 35.0 34.0 37.0 26.0 39.0 76-77 32.48435400527616 35.0 33.0 37.0 25.0 39.0 78-79 32.00469532264143 35.0 33.0 36.0 23.5 38.5 80-81 31.55897026925171 35.0 33.0 36.0 21.0 37.0 82-83 31.129897114861187 35.0 32.0 35.0 19.5 37.0 84-85 30.744349064109542 35.0 32.0 35.0 17.0 36.0 86-87 30.347770821992377 35.0 31.5 35.0 8.5 36.0 88-89 29.929894476780703 34.0 31.0 35.0 2.0 36.0 90-91 29.539787529835433 34.0 31.0 35.0 2.0 35.0 92-93 29.118391440894435 34.0 30.0 35.0 2.0 35.0 94-95 28.65800824923579 34.0 30.0 35.0 2.0 35.0 96-97 28.142449813659397 34.0 29.0 35.0 2.0 35.0 98-99 27.51806879946401 34.0 29.0 35.0 2.0 35.0 100-101 25.40781315690298 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6222190182736789 1101 2 1.7125898615337007 1101 3 0.9857438385953401 1101 4 0.5792098330433504 1101 5 0.43074440552480553 1101 6 0.38109845181659807 1101 7 0.3906908593473375 1101 8 0.3977458439987984 1101 9 0.39783007679538684 1101 10-11 0.4169021906614674 1101 12-13 0.4537865533707688 1101 14-15 0.5037357662796751 1101 16-17 0.4999641388137448 1101 18-19 0.48972030296026503 1101 20-21 0.49688883243378257 1101 22-23 0.49964408236049707 1101 24-25 0.5015590585171097 1101 26-27 0.5615396819888616 1101 28-29 0.5732662301741129 1101 30-31 0.5347461045994208 1101 32-33 0.5291408185643292 1101 34-35 0.5793798759280335 1101 36-37 0.573459470653944 1101 38-39 0.6237879490154157 1101 40-41 0.6432107845878079 1101 42-43 0.7374478109913412 1101 44-45 0.6417220637202661 1101 46-47 0.6385061608807803 1101 48-49 0.6460608882332508 1101 50-51 0.39584439044416087 1101 52-53 0.5342743908155398 1101 54-55 0.6150307755937234 1101 56-57 0.7073227348323528 1101 58-59 0.6860750476988002 1101 60-61 0.6179016451208881 1101 62-63 0.6523449347009489 1101 64-65 0.6852266560628948 1101 66-67 0.6825258055434773 1101 68-69 0.6564461262772525 1101 70-71 0.6130242787837332 1101 72-73 0.6711923812892593 1101 74-75 0.5703936468924056 1101 76-77 0.6790546029010045 1101 78-79 0.6443220022446781 1101 80-81 0.6838625299840864 1101 82-83 0.6781842343194917 1101 84-85 0.5979480653351565 1101 86-87 0.5497175054577426 1101 88-89 0.623716341843565 1101 90-91 0.5703069176194973 1101 92-93 0.5399001351683168 1101 94-95 0.5822766642077184 1101 96-97 0.548068509065601 1101 98-99 0.547721074655648 1101 100-101 0.5827436936778518 1102 1 0.37637088296021304 1102 2 0.9121379389486535 1102 3 0.5727549051235528 1102 4 0.33578201738345115 1102 5 0.2893818316064909 1102 6 0.2626328467539878 1102 7 0.2637354952128632 1102 8 0.28497085146508994 1102 9 0.2690972029594789 1102 10-11 0.3358023161874115 1102 12-13 0.3704444935348832 1102 14-15 0.3815276025746286 1102 16-17 0.3888473598575999 1102 18-19 0.3652339454095568 1102 20-21 0.37227407063117113 1102 22-23 0.36407205596246683 1102 24-25 0.3866093011223555 1102 26-27 0.4292209110087981 1102 28-29 0.4107008500975198 1102 30-31 0.37441947525538666 1102 32-33 0.33137826498580836 1102 34-35 0.39369749022673517 1102 36-37 0.5138447203849026 1102 38-39 0.430095465063026 1102 40-41 0.4511843672172233 1102 42-43 0.5951237783288121 1102 44-45 0.4432027977058013 1102 46-47 0.47381591559431513 1102 48-49 0.39658919358139855 1102 50-51 0.24011513536759566 1102 52-53 0.35848812191049717 1102 54-55 0.4933764182991993 1102 56-57 0.49770942117121564 1102 58-59 0.5687700335538963 1102 60-61 0.5873921639157871 1102 62-63 0.5596145913302806 1102 64-65 0.5572230854731686 1102 66-67 0.5345472706026584 1102 68-69 0.44207317967673276 1102 70-71 0.4508918146473704 1102 72-73 0.48871547997685383 1102 74-75 0.4921170376914077 1102 76-77 0.5348738944521543 1102 78-79 0.5277521090143296 1102 80-81 0.4453382115875435 1102 82-83 0.4558996997241387 1102 84-85 0.441829891495491 1102 86-87 0.3845267795606304 1102 88-89 0.4279187608809565 1102 90-91 0.3613909269896425 1102 92-93 0.3674976715163645 1102 94-95 0.3186122078479663 1102 96-97 0.3448159592307434 1102 98-99 0.42867261724348893 1102 100-101 0.5397498353716905 1103 1 0.33592847594706043 1103 2 1.3793447860805585 1103 3 0.671923338899731 1103 4 0.3303957604053096 1103 5 0.2786090068647127 1103 6 0.18629961226724134 1103 7 0.13928139413117435 1103 8 0.16190756586235722 1103 9 0.23787071136214877 1103 10-11 0.21661012428822346 1103 12-13 0.24441676370766885 1103 14-15 0.25659254834305756 1103 16-17 0.28572575560909286 1103 18-19 0.21907392612540377 1103 20-21 0.1809505901051267 1103 22-23 0.24256593167393703 1103 24-25 0.2551343163642841 1103 26-27 0.29956837238185585 1103 28-29 0.30052884595040297 1103 30-31 0.257158428813419 1103 32-33 0.23007934205745073 1103 34-35 0.27885157465031085 1103 36-37 0.22369177226028114 1103 38-39 0.1798422420830761 1103 40-41 0.36679846641604996 1103 42-43 0.4854042610603315 1103 44-45 0.31486587706792335 1103 46-47 0.18700246258740094 1103 48-49 0.2241973037589844 1103 50-51 0.26980294898795876 1103 52-53 0.30212290994840885 1103 54-55 0.39052674295748346 1103 56-57 0.4838507610712952 1103 58-59 0.4257617655510373 1103 60-61 0.4579645541895303 1103 62-63 0.3653024631095363 1103 64-65 0.3924060334694488 1103 66-67 0.37773259646074564 1103 68-69 0.3560151017301152 1103 70-71 0.39156166288392313 1103 72-73 0.4329839050376023 1103 74-75 0.45000988718582136 1103 76-77 0.4370478734142935 1103 78-79 0.5122080633855148 1103 80-81 0.4519260660770321 1103 82-83 0.500987371303772 1103 84-85 0.45468364770271563 1103 86-87 0.3263596207105408 1103 88-89 0.31539008713939864 1103 90-91 0.25988869995119757 1103 92-93 0.3257330654213533 1103 94-95 0.2993742288460446 1103 96-97 0.10536993638798009 1103 98-99 0.19233610825359193 1103 100-101 0.2831753950560447 1104 1 0.548789536495871 1104 2 1.5278013026605208 1104 3 0.8172414383691802 1104 4 0.5166157654639889 1104 5 0.3998630568649304 1104 6 0.27585337532259047 1104 7 0.29697853603965996 1104 8 0.2797100858773618 1104 9 0.23175633994080158 1104 10-11 0.271828498553667 1104 12-13 0.26162794732945116 1104 14-15 0.2798643367702951 1104 16-17 0.27645862931300513 1104 18-19 0.26638426669779847 1104 20-21 0.3222057296161154 1104 22-23 0.27445000884277704 1104 24-25 0.30320291756388684 1104 26-27 0.3060058214240442 1104 28-29 0.3471792072867501 1104 30-31 0.194931874869404 1104 32-33 0.2282791919474363 1104 34-35 0.20826244404247518 1104 36-37 0.28929770139097144 1104 38-39 0.38172841320272966 1104 40-41 0.34827455294496446 1104 42-43 0.40310649000756626 1104 44-45 0.2909081739822099 1104 46-47 0.3176730383870705 1104 48-49 0.31722467695647794 1104 50-51 0.24707846090070262 1104 52-53 0.2532154141286256 1104 54-55 0.38318406722194 1104 56-57 0.411156802863367 1104 58-59 0.4174624741854771 1104 60-61 0.40562099325634193 1104 62-63 0.43684627773192375 1104 64-65 0.44348160236047107 1104 66-67 0.45318868113945143 1104 68-69 0.3320247921788919 1104 70-71 0.3132161881459794 1104 72-73 0.3422574761587214 1104 74-75 0.26475812329432813 1104 76-77 0.2666578393903052 1104 78-79 0.2996028761703968 1104 80-81 0.23758021454263556 1104 82-83 0.2532537695759878 1104 84-85 0.2059833341692645 1104 86-87 0.1486908175523851 1104 88-89 0.15395210305964113 1104 90-91 0.14003266161851258 1104 92-93 0.06972418410039438 1104 94-95 0.03419676149644246 1104 96-97 -0.027543005997962666 1104 98-99 -0.03926250243616991 1104 100-101 -0.06063902979576952 1105 1 0.30893910021439197 1105 2 1.2996523359210883 1105 3 0.5976451699908267 1105 4 0.33189422853696016 1105 5 0.22416656196708118 1105 6 0.08704126975587911 1105 7 0.07804855085686313 1105 8 0.06586575552877605 1105 9 -0.011937179502858442 1105 10-11 -0.0018744960569208047 1105 12-13 -0.09898721272278266 1105 14-15 -0.044370541306456346 1105 16-17 -0.142359930666494 1105 18-19 -0.1325655722459942 1105 20-21 -0.07315713588474182 1105 22-23 -0.14055104187734457 1105 24-25 -0.10332824264613549 1105 26-27 -0.015858611941254708 1105 28-29 -0.08031047551015291 1105 30-31 -0.08836758587188598 1105 32-33 -0.10315067919111698 1105 34-35 -0.06567907515583471 1105 36-37 -0.05747601592063489 1105 38-39 -0.034239539841216526 1105 40-41 -0.12441187581356417 1105 42-43 0.01902605046415573 1105 44-45 0.006864762696373816 1105 46-47 -0.06264577925298909 1105 48-49 -0.14572309915947557 1105 50-51 -0.1996754327591006 1105 52-53 -0.21355127835983012 1105 54-55 -0.0355822896170821 1105 56-57 -0.057646307619208415 1105 58-59 -0.06478793227731217 1105 60-61 -0.15318500286726078 1105 62-63 -0.09644619661704468 1105 64-65 -0.0207550439884443 1105 66-67 -0.10250459162064374 1105 68-69 -0.26157465191760565 1105 70-71 -0.1932473595218056 1105 72-73 -0.3878775286293177 1105 74-75 -0.4143304031400419 1105 76-77 -0.4082535052958747 1105 78-79 -0.2603426773271629 1105 80-81 -0.2909480319748674 1105 82-83 -0.2964104092591917 1105 84-85 -0.29702833804594064 1105 86-87 -0.29860391441125245 1105 88-89 -0.35841258580260416 1105 90-91 -0.47695622159771034 1105 92-93 -0.611639466478799 1105 94-95 -0.6178983762362549 1105 96-97 -0.8331058938401377 1105 98-99 -0.9542411225626353 1105 100-101 -0.9797168465862214 1106 1 0.23773530537464538 1106 2 1.2569252869416943 1106 3 0.5164195655359194 1106 4 0.2587177462206327 1106 5 0.052052400243361774 1106 6 0.136150026841662 1106 7 0.14986059674149743 1106 8 0.16646191157600754 1106 9 0.011866351490304794 1106 10-11 0.12289132478996834 1106 12-13 0.06070044378151351 1106 14-15 0.07724857538547525 1106 16-17 -0.011067603658062808 1106 18-19 -0.06395909431415703 1106 20-21 -0.19892876184673725 1106 22-23 -0.1614112722861023 1106 24-25 -0.08931003704673657 1106 26-27 -0.025168636150851853 1106 28-29 -0.14895264151555665 1106 30-31 -0.04002468541536075 1106 32-33 -0.03555351573577781 1106 34-35 -0.09432821019541393 1106 36-37 -0.04242835801390754 1106 38-39 -0.021500061900866285 1106 40-41 -1.9887778014293644E-4 1106 42-43 0.15034569801505882 1106 44-45 0.032153130735174784 1106 46-47 -0.03205208082466271 1106 48-49 -0.12973198059882662 1106 50-51 -0.3675605024196287 1106 52-53 -0.15025012671779336 1106 54-55 -0.11220798222091588 1106 56-57 0.03436441305348836 1106 58-59 -0.10382014436940068 1106 60-61 -0.05097821802699798 1106 62-63 -0.04696649265201103 1106 64-65 -0.2051573330399279 1106 66-67 -0.04292590488746839 1106 68-69 -0.042793285206492726 1106 70-71 -0.15549330932723393 1106 72-73 -0.13367309433965602 1106 74-75 -0.2306299494706252 1106 76-77 -0.2843197228785215 1106 78-79 -0.26069267053105705 1106 80-81 -0.3097363165556395 1106 82-83 -0.26587593130445697 1106 84-85 -0.2732007744867495 1106 86-87 -0.351315313852659 1106 88-89 -0.5278106124968929 1106 90-91 -0.6541688827734333 1106 92-93 -0.8642632040403555 1106 94-95 -0.7660082354173063 1106 96-97 -0.8714039770458015 1106 98-99 -0.7732296186709853 1106 100-101 -0.7145536523848293 1107 1 0.4612261877131907 1107 2 1.5161062030304606 1107 3 0.800908438400441 1107 4 0.4351420082839539 1107 5 0.3298374342642276 1107 6 0.29469843299675347 1107 7 0.2256282759309869 1107 8 0.27977250315628055 1107 9 0.23447769227465187 1107 10-11 0.3227285120777239 1107 12-13 0.36581067520958044 1107 14-15 0.35173311532289375 1107 16-17 0.3635252786587344 1107 18-19 0.38784470160245377 1107 20-21 0.3257132375550569 1107 22-23 0.28226045806592026 1107 24-25 0.2881037451420312 1107 26-27 0.37291970862895596 1107 28-29 0.3873888118569724 1107 30-31 0.2895317228599268 1107 32-33 0.31952478721208877 1107 34-35 0.41306660894404246 1107 36-37 0.38097551812966657 1107 38-39 0.3813244165723262 1107 40-41 0.26358431048694086 1107 42-43 0.35961383353936327 1107 44-45 0.21410049536267906 1107 46-47 0.10625899181016507 1107 48-49 0.16396992076727912 1107 50-51 0.19652255363020998 1107 52-53 0.21668112803943984 1107 54-55 0.28172546101114904 1107 56-57 0.34899084346820075 1107 58-59 0.352764838627337 1107 60-61 0.3309794478380894 1107 62-63 0.28678903174414216 1107 64-65 0.24199252786456782 1107 66-67 0.28704892484246614 1107 68-69 0.21174866313480578 1107 70-71 0.15213386320508704 1107 72-73 0.2231100518048521 1107 74-75 0.23175031089630949 1107 76-77 0.22610690722638083 1107 78-79 0.2393300886046461 1107 80-81 0.23071794103791632 1107 82-83 0.2495266169668433 1107 84-85 0.13025813005338804 1107 86-87 0.19297819871432154 1107 88-89 0.3189378669967837 1107 90-91 0.22846325998221317 1107 92-93 0.07049791033101371 1107 94-95 0.08100514242691403 1107 96-97 0.1394882805185702 1107 98-99 0.16335296430819213 1107 100-101 0.060274326144291734 1108 1 0.26648520706308076 1108 2 1.3605784533421783 1108 3 0.6291728404182138 1108 4 0.2689547208066827 1108 5 0.14740500340079876 1108 6 0.09921006599698501 1108 7 0.05423536652002525 1108 8 0.09187345106035139 1108 9 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quality scores pass #Quality Count 2 101276.0 3 26622.0 4 20828.0 5 16980.0 6 15671.0 7 15556.0 8 16863.0 9 18679.0 10 21326.0 11 25087.0 12 29165.0 13 33665.0 14 34764.0 15 37722.0 16 44222.0 17 53321.0 18 49840.0 19 43054.0 20 45105.0 21 48712.0 22 50933.0 23 56797.0 24 65960.0 25 76077.0 26 88444.0 27 106343.0 28 129765.0 29 159268.0 30 195566.0 31 242349.0 32 306807.0 33 398658.0 34 557309.0 35 849734.0 36 1530377.0 37 2972855.0 38 3027032.0 39 427744.0 40 24.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.97457230424342 18.327368824521095 13.921679767468792 24.7763791037667 2 17.39940193290004 20.955953764180965 37.29935314700843 24.34529115591056 3 18.13411196477957 25.579351975861375 29.83037731937303 26.456158739986023 4 13.549438709662823 17.62181570315715 36.789294744069224 32.03945084311081 5 15.02178126355463 35.848053060740156 33.330654530657014 15.799511145048198 6 31.307920799708654 35.17555859094803 19.27349422253581 14.2430263868075 7 28.622738621798 31.225465187211928 22.418416499405346 17.73337969158473 8 27.293442850066263 32.95848126194743 21.140497576212727 18.607578311773572 9 27.3876796794721 16.71212665736612 20.116060541367393 35.78413312179439 10-11 25.444288738073993 25.268962024816027 28.06595601240592 21.22079322470406 12-13 26.545955027493847 22.756819183909233 27.763062759409085 22.934163029187836 14-15 23.775180273201332 24.0528319749017 25.054709246181954 27.117278505715014 16-17 24.03184788507638 26.60871806050557 25.77920311004332 23.580230944374733 18-19 24.189010709701 25.798831503717352 26.8135385927939 23.198619193787746 20-21 24.496348455882163 25.48538659797377 26.472393207241186 23.545871738902875 22-23 24.339640864344037 25.44197126475824 26.54543249241948 23.672955378478246 24-25 24.321947382311144 25.77703418297954 26.231748022986036 23.669270411723275 26-27 24.15951331224976 25.98661513202694 25.8884507227229 23.965420833000394 28-29 24.08835178009104 25.865079698805243 26.000831607658093 24.045736913445626 30-31 24.12857320313442 25.539657109199084 26.172162572800907 24.159607114865594 32-33 23.88893777480265 25.802358499396878 26.194623631910485 24.114080093889992 34-35 24.536802212771047 25.610128358178198 25.892483847898877 23.960585581151875 36-37 24.31194143774661 25.396966206145994 25.972604481632782 24.31848787447461 38-39 24.233739038899483 25.456706152572213 26.20443277209132 24.10512203643699 40-41 24.359707132438267 24.941040782934515 26.65751472985803 24.04173735476919 42-43 23.938330949203994 25.257911673567502 26.510430776999737 24.29332660022877 44-45 23.79386925220143 25.520838634315403 26.224603819521715 24.46068829396146 46-47 24.008138782295237 25.345765904176194 26.23119750397051 24.414897809558056 48-49 23.91547829243666 25.505209556379516 26.105815929719896 24.473496221463932 50-51 23.455236718845445 25.71125966868923 26.225577334206513 24.607926278258816 52-53 23.88918711717462 25.34579241675222 26.055825152250684 24.709195313822473 54-55 23.480469107434097 25.270140477194047 26.373123793263236 24.876266622108623 56-57 23.505560387057315 25.638145617973084 26.323834972204974 24.532459022764623 58-59 23.894286291501956 25.330498775617144 26.045993844079725 24.729221088801175 60-61 23.74090565848408 25.097922149509227 26.51000630415652 24.651165887850173 62-63 23.912673904722386 25.10943970231446 26.32691254410875 24.65097384885441 64-65 23.738808177958138 25.174211810603364 26.021813394302928 25.06516661713557 66-67 23.588700836612865 25.78054235866989 25.716915302344738 24.91384150237251 68-69 23.60383272568719 25.16816080811178 26.194367602984137 25.033638863216893 70-71 23.64914076494557 25.275737996445873 25.91256161400743 25.162559624601123 72-73 23.740871083417193 25.523010344143852 25.63960531365123 25.096513258787724 74-75 23.904237167039653 25.524313566941743 25.699802852610993 24.871646413407614 76-77 24.0550098985776 25.4094396408811 25.88559109469043 24.649959365850876 78-79 23.866742655256648 25.57514963657221 25.852855677602278 24.70525203056886 80-81 24.126850634542162 25.6073331387189 25.850746368674837 24.415069858064093 82-83 24.143371265186666 25.565725720493717 25.860532067849313 24.430370946470312 84-85 24.109630982322923 25.584604333722154 25.772918320206234 24.53284636374869 86-87 24.417630483190784 25.500371185173904 25.739404887380356 24.342593444254955 88-89 24.25776458572053 25.35208890264163 25.736045005860785 24.65410150577706 90-91 24.46560058760228 25.455369988220145 25.90358594546216 24.175443478715415 92-93 24.776845088509724 25.229370155508462 25.772997670215673 24.220787085766133 94-95 24.779266285020398 25.10416877948906 25.647550380442137 24.469014555048403 96-97 25.173390650797067 25.031073574554384 25.387731777640667 24.407803997007885 98-99 24.914510411572262 24.795157791917436 25.568940001197088 24.721391795313213 100-101 25.00123420686682 24.656917731583935 25.729160616119422 24.612687445429827 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 681.0 1 548.5 2 1171.5 3 1619.5 4 987.5 5 700.0 6 899.0 7 1559.0 8 2563.0 9 3124.5 10 2983.0 11 2833.0 12 3059.0 13 3399.0 14 3668.0 15 3946.0 16 4206.5 17 4503.0 18 4947.5 19 5444.5 20 6131.5 21 7040.5 22 8402.5 23 10190.5 24 12332.0 25 15334.0 26 19449.5 27 24753.5 28 31103.5 29 39597.5 30 52271.0 31 70333.0 32 91210.0 33 111980.5 34 139722.5 35 167216.0 36 191828.5 37 218234.5 38 240522.0 39 250515.5 40 261752.5 41 296170.5 42 335677.0 43 372195.0 44 403960.5 45 426702.5 46 444620.5 47 469268.0 48 508188.5 49 535135.5 50 549858.0 51 560689.5 52 551848.0 53 546894.5 54 548032.0 55 521392.0 56 487508.0 57 447957.5 58 400381.5 59 348719.5 60 283125.0 61 217840.5 62 167901.0 63 130261.0 64 97344.0 65 73001.0 66 54190.5 67 41207.0 68 29065.5 69 19015.5 70 13737.0 71 9737.0 72 7004.5 73 5034.0 74 3695.5 75 2816.0 76 2052.0 77 1334.5 78 843.5 79 606.0 80 445.0 81 343.0 82 297.5 83 248.0 84 205.5 85 169.5 86 162.0 87 156.0 88 139.0 89 116.5 90 94.0 91 94.5 92 97.0 93 88.5 94 86.5 95 81.0 96 66.5 97 57.5 98 50.0 99 38.5 100 23.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059746241782169925 2 0.056295800008374856 3 0.062191700515053815 4 0.061731083287969515 5 0.06120346719149114 6 0.011322808927599346 7 0.013374649302793015 8 0.014622503245257738 9 0.00583727649595913 10-11 0.011398182655667685 12-13 0.015309241656547045 14-15 0.01810225702441271 16-17 0.03304719232862945 18-19 0.03103303881746996 20-21 0.03099116452409866 22-23 0.01957204472174532 24-25 0.007562497382856664 26-27 0.011272559775553788 28-29 0.018240442192538002 30-31 0.01630584983878397 32-33 0.01904024119592982 34-35 0.02419496670993677 36-37 0.022679117289895735 38-39 0.00985302123026674 40-41 0.011012939156651733 42-43 0.01239897826724174 44-45 0.023897659227000545 46-47 0.0155688622754491 48-49 0.015204555923118798 50-51 0.014819312424102843 52-53 0.01233616682718479 54-55 0.00852141870105942 56-57 0.01250366400066999 58-59 0.011925798752146057 60-61 0.012993593233114192 62-63 0.021217704451237385 64-65 0.01567773543821448 66-67 0.01827812905657217 68-69 0.02548050751643566 70-71 0.01896905489719861 72-73 0.025300448054939074 74-75 0.04198735396340187 76-77 0.04988903312256606 78-79 0.037665926887483776 80-81 0.019806540764624597 82-83 0.016624094468405844 84-85 0.022821489887358153 86-87 0.025643817260583728 88-89 0.022419496670993678 90-91 0.026581801432100833 92-93 0.051367195678572924 94-95 0.07857292408190612 96-97 0.09339223650600896 98-99 0.09683011599179264 100-101 0.08174699551945061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.19405E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.15242770567777 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43364971983986 30.288441669512906 2 12.577751696033701 10.100545313499184 3 4.140896098210307 4.988010936603376 4 2.03406256840354 3.2669020090659355 5 1.2380676035059786 2.4855709972257762 6 0.7808019018480711 1.8810655149846232 7 0.5530201805946038 1.5543571974773984 8 0.43727810724291444 1.4046222070677388 9 0.3370419739829545 1.2179748144716558 >10 2.094061652617977 15.952393895211598 >50 0.19800931588724088 5.5236419311678615 >100 0.15374496357375086 12.345506141595695 >500 0.014749733109513486 4.019224507145369 >1k 0.0067157197966522955 4.509580878186649 >5k 1.0626096640825403E-4 0.28632539336340995 >10k+ 4.250438656330161E-5 0.17583659342094612 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6749717348519743E-5 2 8.374858674259871E-6 0.0 0.0 0.0 1.6749717348519743E-5 3 8.374858674259871E-6 0.0 0.0 0.0 1.6749717348519743E-5 4 8.374858674259871E-6 8.374858674259871E-6 0.0 0.0 1.6749717348519743E-5 5 1.6749717348519743E-5 1.6749717348519743E-5 0.0 2.5124576022779617E-5 2.5124576022779617E-5 6 2.5124576022779617E-5 1.6749717348519743E-5 0.0 2.5124576022779617E-5 2.5124576022779617E-5 7 2.5124576022779617E-5 1.6749717348519743E-5 0.0 2.5124576022779617E-5 2.5124576022779617E-5 8 3.3499434697039485E-5 1.6749717348519743E-5 0.0 3.3499434697039485E-5 2.5124576022779617E-5 9 3.3499434697039485E-5 1.6749717348519743E-5 0.0 8.374858674259872E-5 2.5124576022779617E-5 10-11 3.3499434697039485E-5 1.6749717348519743E-5 0.0 2.093714668564968E-4 2.5124576022779617E-5 12-13 5.0249152045559234E-5 2.093714668564968E-5 0.0 3.5593149365604457E-4 7.537372806833885E-5 14-15 5.0249152045559234E-5 2.5124576022779617E-5 0.0 5.527406725011515E-4 9.212344541685859E-5 16-17 5.0249152045559234E-5 2.5124576022779617E-5 0.0 7.788618567061681E-4 1.0049830409111847E-4 18-19 6.699886939407897E-5 2.5124576022779617E-5 0.0 0.0010803567689795235 1.1306059210250828E-4 20-21 8.374858674259872E-5 2.5124576022779617E-5 0.0 0.0016289100121435452 1.2562288011389809E-4 22-23 8.793601607972866E-5 2.5124576022779617E-5 0.0 0.003123822285498932 1.2562288011389809E-4 24-25 1.2562288011389809E-4 3.7686864034169426E-5 0.0 0.006293706293706293 1.2562288011389809E-4 26-27 1.2562288011389809E-4 5.443658138268917E-5 0.0 0.010732381391064026 1.2562288011389809E-4 28-29 1.29810309451028E-4 5.86240107198191E-5 0.0 0.026678112306854823 1.3399773878815794E-4 30-31 1.3399773878815794E-4 5.86240107198191E-5 0.0 0.08162556006867384 1.3399773878815794E-4 32-33 1.3399773878815794E-4 6.699886939407897E-5 0.0 0.19722792177881998 1.507474561366777E-4 34-35 1.4237259746241782E-4 6.699886939407897E-5 0.0 0.33622545119551106 1.507474561366777E-4 36-37 1.4237259746241782E-4 7.118629873120891E-5 0.0 0.543603701687534 1.507474561366777E-4 38-39 1.507474561366777E-4 7.537372806833885E-5 0.0 0.8935094845274486 1.5912231481093756E-4 40-41 1.5912231481093756E-4 7.956115740546878E-5 0.0 1.3096101503287132 1.6330974414806748E-4 42-43 1.716846028223274E-4 8.374858674259872E-5 0.0 1.729261756207864 1.716846028223274E-4 44-45 1.9262174950797706E-4 8.374858674259872E-5 0.0 2.1632385578493363 1.8424689083371717E-4 46-47 2.5124576022779617E-4 8.374858674259872E-5 0.0 2.6300615552112556 1.884343201708471E-4 48-49 2.8474519492483564E-4 8.374858674259872E-5 0.0 3.119659980737825 2.0099660818223694E-4 50-51 2.8474519492483564E-4 8.793601607972866E-5 0.0 3.641476487584272 2.0099660818223694E-4 52-53 2.8474519492483564E-4 9.212344541685859E-5 0.0 4.146359030191366 2.2612118420501655E-4 54-55 2.8474519492483564E-4 1.0049830409111847E-4 0.0 4.642142288848875 2.428709015535363E-4 56-57 3.014949122733554E-4 1.0049830409111847E-4 0.0 5.166839747079268 2.428709015535363E-4 58-59 3.0986977094761526E-4 1.172480214396382E-4 0.0 5.7000251245760225 2.428709015535363E-4 60-61 3.182446296218751E-4 1.29810309451028E-4 0.0 6.263648925924375 2.428709015535363E-4 62-63 3.3080691763326495E-4 1.3399773878815794E-4 0.0 6.83250701394414 2.428709015535363E-4 64-65 3.349943469703949E-4 1.3399773878815794E-4 0.0 7.3807713244838995 2.428709015535363E-4 66-67 3.6849378166743435E-4 1.3399773878815794E-4 0.0 7.942724341526737 2.428709015535363E-4 68-69 3.9361835769021397E-4 1.3399773878815794E-4 0.0 8.534705414346133 2.428709015535363E-4 70-71 3.9361835769021397E-4 1.3399773878815794E-4 0.0 9.14578535237218 2.428709015535363E-4 72-73 3.978057870273439E-4 1.3399773878815794E-4 0.0 9.74404756919727 2.428709015535363E-4 74-75 4.019932163644739E-4 1.3818516812528787E-4 0.0 10.33205477157573 2.428709015535363E-4 76-77 4.019932163644739E-4 1.4237259746241782E-4 0.0 10.93403961308153 2.5124576022779617E-4 78-79 4.019932163644739E-4 1.4237259746241782E-4 0.0 11.547175578912107 2.5124576022779617E-4 80-81 4.438675097357732E-4 1.4237259746241782E-4 0.0 12.187693145178176 2.554331895649261E-4 82-83 4.6061722708429296E-4 1.507474561366777E-4 0.0 12.85153469285206 2.59620618902056E-4 84-85 4.648046564214229E-4 1.5912231481093756E-4 0.0 13.504899292324442 2.59620618902056E-4 86-87 4.7317951509568275E-4 1.6330974414806748E-4 0.0 14.132632636824255 2.679954775763159E-4 88-89 4.7736694443281267E-4 1.6749717348519744E-4 0.0 14.761685021565262 2.763703362505758E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9020 0.0 46.250042 1 TATAACG 915 0.0 43.10075 2 GTATCAA 16035 0.0 41.869457 1 TATCACG 1085 0.0 38.975174 2 TAACGCA 1150 0.0 36.76961 4 ATAACGC 1120 0.0 36.48311 3 TCAACGC 19315 0.0 35.29832 4 ATCAACG 19290 0.0 35.246727 3 CAACGCA 20405 0.0 33.527058 5 ATCACGC 1385 0.0 32.59007 3 TATCAAC 20450 0.0 32.203102 2 AACGCAG 21680 0.0 32.02629 6 TACCTGG 10575 0.0 29.699484 2 GTACATG 30980 0.0 27.821465 1 ACGCAGA 25295 0.0 27.449871 7 CGCAGAG 26330 0.0 26.316187 8 GTATAAC 2385 0.0 26.29721 1 ACATGGG 33470 0.0 25.467022 3 TACATGG 34245 0.0 24.905489 2 GTACCTG 14220 0.0 22.654488 1 >>END_MODULE