FastQCFastQC Report
Fri 27 May 2016
SRR940191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11940500
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132330.11082450483648087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10350.051.8642
GGTATCA94100.050.7452351
ATAACGC11800.045.4901163
GTATCAA174150.042.9710461
TAACGCA14400.038.925874
TATCACG9800.036.839642
TCAACGC208850.034.708744
ATCAACG211000.034.2651633
CAACGCA221850.032.8029335
TATCAAC222150.032.80252
AACGCAG235200.031.4232486
GTATAAC23600.029.9980561
TACCTGG106250.029.4635032
ATCACGC12900.028.3544773
ACGCAGA272000.027.1367157
GTACATG308750.026.6230621
CGCAGAG281350.026.2346738
ACATGGG326900.023.7151493
TACATGG345850.023.3775862
GTACCTG146150.021.9444051
Produced by FastQC (version 0.11.5)