##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11940500 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.02285867425987 34.0 33.0 34.0 31.0 34.0 2 33.088818558686825 34.0 33.0 34.0 31.0 34.0 3 33.140981282190864 34.0 33.0 34.0 31.0 34.0 4 36.46816758092207 37.0 37.0 37.0 35.0 37.0 5 36.44742665717516 37.0 37.0 37.0 35.0 37.0 6 36.470732632636825 37.0 37.0 37.0 35.0 37.0 7 36.448732883882585 37.0 37.0 37.0 35.0 37.0 8 36.444037938109794 37.0 37.0 37.0 35.0 37.0 9 38.288082408609355 39.0 39.0 39.0 37.0 39.0 10-11 38.24579288974499 39.0 39.0 39.0 37.0 39.0 12-13 38.20571161174155 39.0 39.0 39.0 37.0 39.0 14-15 39.72362241949667 41.0 40.0 41.0 37.0 41.0 16-17 39.70714743938696 41.0 40.0 41.0 37.0 41.0 18-19 39.674059042753655 41.0 40.0 41.0 37.0 41.0 20-21 39.61534789162933 41.0 40.0 41.0 37.0 41.0 22-23 39.53932816883715 41.0 39.5 41.0 37.0 41.0 24-25 39.46160596289938 41.0 39.0 41.0 36.5 41.0 26-27 39.288882249487045 41.0 39.0 41.0 36.0 41.0 28-29 39.41483643901009 41.0 39.0 41.0 36.0 41.0 30-31 39.30453159415435 41.0 39.0 41.0 36.0 41.0 32-33 39.17761090406599 41.0 39.0 41.0 35.5 41.0 34-35 39.28795527825468 41.0 39.0 41.0 36.0 41.0 36-37 39.25084540010887 41.0 39.0 41.0 35.5 41.0 38-39 39.15711448431807 41.0 39.0 41.0 35.0 41.0 40-41 39.03382680792262 41.0 39.0 41.0 35.0 41.0 42-43 38.922164272852896 41.0 39.0 41.0 35.0 41.0 44-45 38.79260190109292 41.0 39.0 41.0 35.0 41.0 46-47 38.61190812780035 40.5 38.0 41.0 35.0 41.0 48-49 38.43790025543319 40.0 38.0 41.0 35.0 41.0 50-51 38.248664042544284 40.0 38.0 41.0 34.0 41.0 52-53 38.0529911645241 40.0 37.5 41.0 34.0 41.0 54-55 37.844498806582635 40.0 37.0 41.0 34.0 41.0 56-57 37.6298192705498 40.0 37.0 41.0 33.5 41.0 58-59 37.33018035258155 39.5 36.0 41.0 33.0 41.0 60-61 37.002359407060005 39.0 35.5 41.0 33.0 41.0 62-63 36.6805394665215 39.0 35.0 40.5 32.0 41.0 64-65 36.314979188476194 38.0 35.0 40.0 32.0 41.0 66-67 35.91360943846573 37.0 35.0 40.0 31.0 41.0 68-69 35.47759666680625 36.5 35.0 39.0 31.0 41.0 70-71 35.066412043046775 36.0 35.0 39.0 31.0 40.5 72-73 34.62925627067543 35.5 34.5 38.5 30.0 40.0 74-75 34.20696897114861 35.0 34.0 37.0 30.0 39.0 76-77 32.87392261630585 34.5 32.5 36.0 28.0 38.5 78-79 33.48848507181441 35.0 34.0 36.0 29.0 38.5 80-81 33.31087839705205 35.0 34.0 36.0 29.0 37.0 82-83 33.056292952556426 35.0 34.0 35.5 29.0 37.0 84-85 32.7915659729492 35.0 34.0 35.0 29.0 36.5 86-87 32.52576420585403 35.0 33.0 35.0 29.0 36.0 88-89 32.27210150328713 35.0 33.0 35.0 27.5 36.0 90-91 32.03630354675265 35.0 33.0 35.0 27.0 35.5 92-93 31.79060365981324 35.0 33.0 35.0 26.5 35.0 94-95 31.536947615258992 35.0 33.0 35.0 25.0 35.0 96-97 31.204353293413174 34.5 32.0 35.0 24.5 35.0 98-99 30.773035467526483 34.0 32.0 35.0 23.5 35.0 100-101 28.31078970729869 32.0 28.0 34.5 9.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11289659726056556 1101 2 0.10741317018424468 1101 3 0.12052820638505324 1101 4 0.0636592448650859 1101 5 0.07544459295112205 1101 6 0.09745527859887204 1101 7 0.06320628796205341 1101 8 0.0735440093171249 1101 9 0.09569811517071969 1101 10-11 0.10258290448368257 1101 12-13 0.10089065125615804 1101 14-15 0.15642890872944548 1101 16-17 0.14452200564010553 1101 18-19 0.1471882949298262 1101 20-21 0.13807096303217037 1101 22-23 0.1509989850259359 1101 24-25 0.15958641035884114 1101 26-27 0.21186444422095008 1101 28-29 0.16120145207005265 1101 30-31 0.12485000464965879 1101 32-33 0.1614127685241442 1101 34-35 0.2086947647071966 1101 36-37 0.17813133733847764 1101 38-39 0.19814712542043367 1101 40-41 0.21332254835393627 1101 42-43 0.21863724464520118 1101 44-45 0.25881635243795387 1101 46-47 0.23929647993338676 1101 48-49 0.24254073031614354 1101 50-51 0.25098981911436624 1101 52-53 0.30058990072583924 1101 54-55 0.33378814432943926 1101 56-57 0.26778745547566984 1101 58-59 0.3174020562301578 1101 60-61 0.355794439412513 1101 62-63 0.3275274243346331 1101 64-65 0.28470413217949897 1101 66-67 0.2999055404082469 1101 68-69 0.2999278669947003 1101 70-71 0.3020190010560455 1101 72-73 0.3115336384087186 1101 74-75 0.29623463640145786 1101 76-77 0.22465577001452885 1101 78-79 0.37158073105798195 1101 80-81 0.3463620081363459 1101 82-83 0.33443059830040767 1101 84-85 0.3409992484512685 1101 86-87 0.43567659786016577 1101 88-89 0.4349586118392992 1101 90-91 0.4667506705890787 1101 92-93 0.4563947915237776 1101 94-95 0.43878543267720715 1101 96-97 0.425396440737277 1101 98-99 0.36223586319588463 1101 100-101 0.5402395101894726 1102 1 0.06333253906678493 1102 2 0.05362273704984233 1102 3 0.08267557257223501 1102 4 0.02678847403861795 1102 5 0.03242698905977903 1102 6 0.06526189618137579 1102 7 0.0182649524479217 1102 8 0.05654578304449842 1102 9 0.04182825305387183 1102 10-11 0.05563589811674774 1102 12-13 0.05759613991926926 1102 14-15 0.11216346585595716 1102 16-17 0.1160513797431193 1102 18-19 0.10354641960392286 1102 20-21 0.10446725398533374 1102 22-23 0.09966097048793188 1102 24-25 0.09573267454733525 1102 26-27 0.13601807693590473 1102 28-29 0.058995051305096524 1102 30-31 0.1310069380688006 1102 32-33 0.15651266348724846 1102 34-35 0.19006777786319873 1102 36-37 0.1859390723203802 1102 38-39 0.16814954814856264 1102 40-41 0.2304941513206984 1102 42-43 0.19454024175869478 1102 44-45 0.16074686512141767 1102 46-47 0.16818655971990637 1102 48-49 0.17966666102434914 1102 50-51 0.21359852809946034 1102 52-53 0.22506676173858153 1102 54-55 0.21381754407768483 1102 56-57 0.22363313346029656 1102 58-59 0.23498555690202494 1102 60-61 0.23016377363108376 1102 62-63 0.24393399203783872 1102 64-65 0.22749196870022814 1102 66-67 0.20288096908728903 1102 68-69 0.21114130065924286 1102 70-71 0.23430191789859833 1102 72-73 0.22122035815423402 1102 74-75 0.24225726614767495 1102 76-77 0.13026347313662967 1102 78-79 0.2552459700043812 1102 80-81 0.3307717932985099 1102 82-83 0.26812269689587964 1102 84-85 0.293639238513407 1102 86-87 0.2799357498496846 1102 88-89 0.20416069979538776 1102 90-91 0.21970987933733 1102 92-93 0.2561697189945846 1102 94-95 0.17642909516369798 1102 96-97 0.11705581072483895 1102 98-99 0.19842467947303533 1102 100-101 0.44368879898205904 1103 1 0.04027381781099848 1103 2 0.010117186219346763 1103 3 0.027708437550153064 1103 4 -0.0014254141822220845 1103 5 0.008614318997118176 1103 6 0.030102951744645168 1103 7 -0.04778857307918827 1103 8 0.006854601349353118 1103 9 -0.04421177833512502 1103 10-11 -0.014338488790826887 1103 12-13 0.03401454307047658 1103 14-15 0.005932839131645551 1103 16-17 -0.0014686224112168134 1103 18-19 -0.03550381455362839 1103 20-21 0.006158045501891252 1103 22-23 0.02396689156643106 1103 24-25 -0.03859258276457922 1103 26-27 0.04259374863407572 1103 28-29 -0.012594063629563834 1103 30-31 -0.01552311754265645 1103 32-33 -0.05362422525900712 1103 34-35 -0.05265374348453378 1103 36-37 0.0014546953559246845 1103 38-39 -0.016641431304179832 1103 40-41 -0.003997039405710723 1103 42-43 -0.02099918240585197 1103 44-45 -0.0502566363727297 1103 46-47 0.03837282001757103 1103 48-49 0.027686960793595006 1103 50-51 0.0015430513098309007 1103 52-53 0.024536958148971166 1103 54-55 0.047384168121382686 1103 56-57 0.03764285798877154 1103 58-59 0.03686421488927749 1103 60-61 -0.0023981699494939335 1103 62-63 -0.06044212137218352 1103 64-65 -0.048653747059589136 1103 66-67 -0.026915903362379368 1103 68-69 0.0020633966812724225 1103 70-71 -0.22010077691065533 1103 72-73 -0.08079053486050469 1103 74-75 -0.02669230391671107 1103 76-77 -0.13228092131110003 1103 78-79 0.037419550993099904 1103 80-81 0.15733325353204464 1103 82-83 -0.012525935275007782 1103 84-85 0.03371559733309937 1103 86-87 0.07588101719812812 1103 88-89 -0.020129587092881707 1103 90-91 0.0505723168081289 1103 92-93 0.07823352937121797 1103 94-95 -0.008812758442964252 1103 96-97 -0.015714708559755763 1103 98-99 -0.016854201453249118 1103 100-101 0.06234127827416813 1104 1 0.0345596054241355 1104 2 0.02385460437321285 1104 3 0.01318233084720788 1104 4 0.015505844506357391 1104 5 -0.004339978439453773 1104 6 0.022534515963023694 1104 7 0.03205583127297018 1104 8 0.04621438480960194 1104 9 0.04844466893607802 1104 10-11 0.04692479839364694 1104 12-13 0.027187043117599785 1104 14-15 0.06697380335075565 1104 16-17 0.045222083800616986 1104 18-19 0.09125125324464278 1104 20-21 0.10187206480686228 1104 22-23 0.07130986648331827 1104 24-25 0.07428324249522689 1104 26-27 0.1257480975495895 1104 28-29 0.09510678459097477 1104 30-31 0.07346051228316242 1104 32-33 0.09832000424731291 1104 34-35 0.08754858196381576 1104 36-37 0.08381022768499946 1104 38-39 0.09210037046179309 1104 40-41 0.11330124135457709 1104 42-43 0.1190221088554253 1104 44-45 0.0854667666803195 1104 46-47 -0.0726815874485851 1104 48-49 0.11061057127266594 1104 50-51 0.14653931178195734 1104 52-53 0.08934616161719333 1104 54-55 0.07693205795057878 1104 56-57 0.11414370766857473 1104 58-59 0.10531241461012542 1104 60-61 0.04758858912523323 1104 62-63 0.01911414967674574 1104 64-65 -0.05066406489707731 1104 66-67 -0.04132845895526316 1104 68-69 0.016414403438453462 1104 70-71 -0.018640779902149518 1104 72-73 0.007779466397877854 1104 74-75 -0.02496796075239871 1104 76-77 -0.10385038141446756 1104 78-79 0.07858575733522599 1104 80-81 0.10037238688237693 1104 82-83 -8.873109121125822E-4 1104 84-85 0.016022238682673162 1104 86-87 0.020615204846606616 1104 88-89 0.015525624756904222 1104 90-91 0.03407294742282829 1104 92-93 0.06454245007482484 1104 94-95 0.05617560335483773 1104 96-97 0.015059597778016354 1104 98-99 0.01348241675214723 1104 100-101 0.041289282419946005 1105 1 -0.024417354944311853 1105 2 -0.008982657531703353 1105 3 -0.04251900633542505 1105 4 -0.032452247488791386 1105 5 -0.0034633620920061503 1105 6 -0.02765133115092766 1105 7 -0.028302308975568735 1105 8 -0.02249592504926312 1105 9 -0.060587382937313805 1105 10-11 0.012068355630404426 1105 12-13 -0.01396521337667167 1105 14-15 -0.015018500739046203 1105 16-17 -0.0030077172001412578 1105 18-19 -0.08066912497163514 1105 20-21 -0.055923957714028916 1105 22-23 0.005638349810396903 1105 24-25 0.025431943834973936 1105 26-27 0.055867258697730904 1105 28-29 -0.03687570601953638 1105 30-31 0.03595008394839283 1105 32-33 0.017048369031911648 1105 34-35 -0.010591858275283528 1105 36-37 0.04949329176371009 1105 38-39 -0.04502503540901159 1105 40-41 -0.04875764551272965 1105 42-43 -0.2242144286616039 1105 44-45 -0.15401767645095532 1105 46-47 -0.055425633404482255 1105 48-49 -0.11151565246372996 1105 50-51 -0.23095791495693874 1105 52-53 -0.08361241643719808 1105 54-55 -0.03463434461810522 1105 56-57 0.0016408410538417684 1105 58-59 -0.14658471809729434 1105 60-61 -0.24391112974718254 1105 62-63 -0.05615629557484425 1105 64-65 -0.23569420215853398 1105 66-67 -0.28712833218496314 1105 68-69 -0.39446415381728883 1105 70-71 -0.26737830060543644 1105 72-73 -0.29251301492613635 1105 74-75 -0.25437168279659517 1105 76-77 -0.44845113402026726 1105 78-79 -0.3441050342305161 1105 80-81 -0.2749700881875512 1105 82-83 -0.2942273143651377 1105 84-85 -0.2348778635932689 1105 86-87 -0.33729187142998285 1105 88-89 -0.33569148724568265 1105 90-91 -0.37872274851164534 1105 92-93 -0.4126631986159026 1105 94-95 -0.4654464827654152 1105 96-97 -0.56347072007053 1105 98-99 -0.4953993304744486 1105 100-101 -0.3314177794587003 1106 1 -0.07307371598859191 1106 2 -0.04835501939695064 1106 3 -0.02637174579089674 1106 4 -0.008452192257344393 1106 5 -0.012692449604500666 1106 6 -0.015523281539444156 1106 7 -0.030178603805907755 1106 8 -0.0033784574497133235 1106 9 -0.11418253879194395 1106 10-11 -0.03506451209649697 1106 12-13 -0.017943517290994748 1106 14-15 -0.006895538520872435 1106 16-17 -0.02460082608035208 1106 18-19 -0.03593882099394108 1106 20-21 0.011439743014484804 1106 22-23 -0.18592643106183004 1106 24-25 -0.11697188640013678 1106 26-27 -0.02353766364571186 1106 28-29 -0.10189443588308933 1106 30-31 -0.02251589333810955 1106 32-33 -0.08015642379477583 1106 34-35 -0.08703555195325663 1106 36-37 -0.23584581147358818 1106 38-39 -0.23505802512804053 1106 40-41 -0.16448290862894055 1106 42-43 -0.013682061899331188 1106 44-45 -0.12301755215560917 1106 46-47 -0.017429703755340142 1106 48-49 0.030731703710067393 1106 50-51 -0.01601110363363034 1106 52-53 -0.026670936899783726 1106 54-55 0.022314637409216687 1106 56-57 0.03360839707330854 1106 58-59 -0.08365896973151621 1106 60-61 -0.10608810666208512 1106 62-63 -0.19926688085752886 1106 64-65 -0.03874093215075902 1106 66-67 -0.15302852835745284 1106 68-69 -0.02227713758696126 1106 70-71 0.023438071231275615 1106 72-73 0.01467403586484295 1106 74-75 -0.034345329132385416 1106 76-77 -0.2235717845122167 1106 78-79 -0.07134668822501311 1106 80-81 0.06643851667312362 1106 82-83 0.058270864313193727 1106 84-85 -0.01049672041207117 1106 86-87 0.06592710343127095 1106 88-89 0.07727673956198089 1106 90-91 0.13658539338472053 1106 92-93 -0.021774742200229014 1106 94-95 -0.0530087721878445 1106 96-97 -0.05938708201790277 1106 98-99 -0.23107502533682123 1106 100-101 -0.3037578825606211 1107 1 0.026700212625094366 1107 2 0.04027059116335607 1107 3 0.03896777220618475 1107 4 -0.00878747515522349 1107 5 0.04903735021737532 1107 6 0.03514482036763411 1107 7 0.0417372230466313 1107 8 0.04949544129412686 1107 9 -0.034747829234291316 1107 10-11 0.038768079664961874 1107 12-13 0.05708865879082481 1107 14-15 0.03482781375510058 1107 16-17 0.10289220883583283 1107 18-19 0.1532520781895883 1107 20-21 0.1412054777329459 1107 22-23 0.11588732854566786 1107 24-25 0.1331633899812772 1107 26-27 0.16093137683519387 1107 28-29 0.1615487751617266 1107 30-31 0.1409901337297299 1107 32-33 0.1469247981031927 1107 34-35 0.09796296183071007 1107 36-37 0.035074053569182695 1107 38-39 0.10964703870688908 1107 40-41 0.13544049353925658 1107 42-43 0.158571735086511 1107 44-45 0.144130486401032 1107 46-47 0.1286060994778211 1107 48-49 0.07419813594551528 1107 50-51 0.009399850358590811 1107 52-53 0.14615220735445433 1107 54-55 0.17786838799956683 1107 56-57 0.1960415461546674 1107 58-59 0.23160699037515542 1107 60-61 0.09845265723395613 1107 62-63 0.08159855468137778 1107 64-65 0.09488299653771293 1107 66-67 0.10914875629494247 1107 68-69 0.11400279335462926 1107 70-71 0.07523614098167997 1107 72-73 0.20041292393917587 1107 74-75 -0.01132949625251456 1107 76-77 -0.04700531322242085 1107 78-79 0.22173616869250878 1107 80-81 0.27743719037741243 1107 82-83 0.28993954777076425 1107 84-85 0.25385901878505024 1107 86-87 0.2412520244544396 1107 88-89 0.21829713469858092 1107 90-91 0.32083242617756724 1107 92-93 0.38479471061719295 1107 94-95 0.3684530634702554 1107 96-97 0.3406316306000683 1107 98-99 0.3282879911242631 1107 100-101 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-0.3315796137968121 2316 56-57 -0.2850778385076964 2316 58-59 -0.4765322274269437 2316 60-61 -0.3326499550899982 2316 62-63 -0.3012860777645656 2316 64-65 -0.539503710141851 2316 66-67 -0.49039614680547317 2316 68-69 -0.38019733889839813 2316 70-71 -0.2773573130093254 2316 72-73 -0.41535223168680346 2316 74-75 -0.32609238645223826 2316 76-77 -0.3300193961408766 2316 78-79 -0.5414011027962076 2316 80-81 -0.5008873002971299 2316 82-83 -0.5363040123734137 2316 84-85 -0.3859150816295909 2316 86-87 -0.5238129053854266 2316 88-89 -0.4054786823232064 2316 90-91 -0.4205625303261087 2316 92-93 -0.5470605077378536 2316 94-95 -0.4749688185253831 2316 96-97 -0.4959881698030877 2316 98-99 -0.3406302179785712 2316 100-101 -0.5538387589965623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 14.0 4 32.0 5 136.0 6 256.0 7 563.0 8 1352.0 9 2845.0 10 4685.0 11 7796.0 12 12424.0 13 17407.0 14 18507.0 15 22058.0 16 29095.0 17 41518.0 18 39144.0 19 28184.0 20 27196.0 21 31039.0 22 30615.0 23 33823.0 24 40088.0 25 48849.0 26 58089.0 27 70918.0 28 88255.0 29 111218.0 30 138509.0 31 175375.0 32 225976.0 33 302848.0 34 440950.0 35 706370.0 36 1395661.0 37 3276144.0 38 3965261.0 39 547264.0 40 29.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.65736362835247 18.608620349388318 14.191852296871174 24.542163725388033 2 16.18099998927999 20.884194753091386 38.096844900223516 24.837960357405116 3 18.17986987405481 25.77933528703149 29.90737688552642 26.133417953387283 4 13.751908057467265 17.9640145344859 36.64117732484857 31.642900083198256 5 14.977932797171862 35.98794181099647 33.29928092092777 15.734844470903909 6 31.252807173097235 35.42939499562535 19.13618756099569 14.181610270281725 7 28.499166320294645 31.387980188664788 22.379783922481227 17.733069568559344 8 27.251111762489007 33.09673799254638 21.099786441103806 18.55236380386081 9 27.281939617268957 16.7168041539299 20.12287592646874 35.878380302332396 10-11 25.400912859595493 25.39524726770236 27.99062015828483 21.21321971441732 12-13 26.571932773834128 22.807638826164684 27.76898906113601 22.85143933886518 14-15 23.71077844311377 24.12053096603995 25.098408776851887 27.07028181399439 16-17 23.95353209664587 26.682877601440474 25.823081110506262 23.540509191407395 18-19 24.138947280264645 25.799020141535113 26.93574389682174 23.1262886813785 20-21 24.42865202077288 25.46544927615856 26.635535934789615 23.47036276827895 22-23 24.27747228370419 25.456841461368413 26.70022809358759 23.565458161339812 24-25 24.27792029319563 25.77054323073214 26.371372003681444 23.580164472390784 26-27 24.116378681325408 25.993238195389846 26.05398966697365 23.836393456311093 28-29 24.0189525687057 25.861292626624007 26.155829523695505 23.96392528097479 30-31 24.120982746128412 25.524238787897925 26.29532591216291 24.059452553810758 32-33 23.868432453591645 25.812180218001785 26.32116817908604 23.998219149320526 34-35 24.531399413388748 25.59494427595083 26.0318249418824 23.841831368778017 36-37 24.26482814992518 25.389361222352058 26.115801178727132 24.23000944899563 38-39 24.175528662953813 25.441794732213896 26.369260081236128 24.013416523596163 40-41 24.323524296929865 24.90785224148849 26.797995673453208 23.970627788128432 42-43 23.833202127214104 25.278803232695445 26.647996315062183 24.239998325028264 44-45 23.769322715919987 25.487101718758865 26.324260460087444 24.419315105233704 46-47 23.981808685079002 25.352430344648297 26.32333202135497 24.34242894891773 48-49 23.914460849034917 25.490351754139347 26.184648415453577 24.410538981372166 50-51 23.399702692517064 25.68886562539257 26.280457267283612 24.63097441480675 52-53 23.850430438748287 25.354217008329055 26.092071925685467 24.703280627237188 54-55 23.443329939947073 25.272316420992674 26.36208878885674 24.92226485020351 56-57 23.3901982073698 25.62226144727028 26.281132543910747 24.706407801449178 58-59 23.727375635480453 25.357063938879808 26.026953621227833 24.888606804411904 60-61 23.649130203345052 25.162832486299557 26.386936149699697 24.80110116065569 62-63 23.72090958750149 25.22156272357008 26.141045147520398 24.91648254140803 64-65 23.447100814046227 25.306432513742827 25.81668646109108 25.42978021111987 66-67 23.298342786205193 25.92135246346812 25.53650976515735 25.243794985169338 68-69 23.28877734677902 25.312573201603318 25.965532550065184 25.433116901552477 70-71 23.34410298329647 25.40855179467157 25.61308260177893 25.63426262025303 72-73 23.37509058622657 25.712585826078964 25.29419533761391 25.618128250080545 74-75 23.421194651741082 25.667544129701948 25.30207736972806 25.60918384882891 76-77 23.541442760974203 25.56809478613131 25.456378181079977 25.434084271814513 78-79 23.341627235040406 25.81279678405427 25.335480088773505 25.51009589213182 80-81 23.433839597748822 25.930075203302845 25.309849893633007 25.326235305315326 82-83 23.332978935850303 25.84929039755483 25.36600054595916 25.451730120635702 84-85 23.27115901854325 25.757509075570074 25.267711154062273 25.703620751824403 86-87 23.4615805136456 25.72550748139842 25.25689754654285 25.556014458413134 88-89 23.218660157391238 25.675429239790176 25.272305400624152 25.83360520219443 90-91 23.326172718223933 25.751615430581186 25.467611197862034 25.454600653332847 92-93 23.44905118447573 25.52973294788494 25.3969759529435 25.624239914695824 94-95 23.25277417193585 25.339156651731503 25.4030903228508 26.00497885348185 96-97 23.561977961264315 25.271964627942705 25.283237295814274 25.8828201149787 98-99 23.229709597800056 25.169566931143493 25.50019821210877 26.100525258947677 100-101 23.029468552948426 25.16019159421385 25.72304503438932 26.08729481844841 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 482.0 1 437.5 2 1271.0 3 1764.0 4 954.5 5 597.5 6 892.0 7 1673.0 8 2740.5 9 3315.5 10 3139.0 11 2902.0 12 3079.0 13 3417.5 14 3809.0 15 4124.5 16 4311.0 17 4619.5 18 5001.0 19 5390.0 20 6004.0 21 6960.0 22 8317.0 23 10196.5 24 12533.0 25 15563.0 26 19847.5 27 25543.5 28 31743.5 29 40086.5 30 52851.5 31 70738.0 32 91769.0 33 111833.5 34 138598.5 35 165618.5 36 189378.5 37 214770.5 38 236576.5 39 245554.5 40 255303.5 41 290319.5 42 331622.0 43 370034.5 44 404243.0 45 427812.0 46 444705.5 47 470683.0 48 511663.0 49 538654.0 50 554784.5 51 565640.5 52 554919.0 53 551892.0 54 555749.0 55 527968.0 56 494006.0 57 454823.5 58 405833.0 59 352093.0 60 284166.5 61 216427.0 62 165134.5 63 127582.5 64 95137.5 65 70861.5 66 51099.0 67 38726.5 68 26941.5 69 16638.5 70 11546.0 71 7851.5 72 5404.5 73 3841.0 74 2911.5 75 2160.0 76 1381.0 77 754.5 78 404.0 79 214.0 80 120.5 81 83.5 82 56.0 83 34.5 84 25.0 85 23.0 86 18.0 87 12.0 88 10.5 89 11.0 90 6.5 91 3.0 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021841631422469747 2 0.0017754700389430928 3 5.694903898496713E-4 4 0.002897701101293916 5 0.008894099912063984 6 8.961098781458063E-4 7 5.27616096478372E-4 8 0.0 9 0.0 10-11 0.0 12-13 4.187429337129936E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0026464553410661196 22-23 5.443658138268917E-5 24-25 6.406766885808802E-4 26-27 4.0618064570160375E-4 28-29 5.066789497927222E-4 30-31 1.7168460282232737E-4 32-33 0.0011641053557221222 34-35 4.522423684100331E-4 36-37 6.490515472551401E-4 38-39 0.0 40-41 2.554331895649261E-4 42-43 0.0 44-45 4.396800803986433E-4 46-47 0.004325614505255224 48-49 0.0011976047904191617 50-51 0.0 52-53 7.537372806833885E-5 54-55 9.75671035551275E-4 56-57 4.187429337129936E-6 58-59 2.219337548678866E-4 60-61 6.699886939407897E-5 62-63 2.721829069134458E-4 64-65 0.0022528369833759053 66-67 3.0986977094761526E-4 68-69 1.4237259746241782E-4 70-71 4.187429337129936E-6 72-73 0.0018340940496629121 74-75 1.3399773878815794E-4 76-77 1.5912231481093756E-4 78-79 0.0 80-81 4.187429337129936E-6 82-83 3.8943092835308404E-4 84-85 5.485532431640216E-4 86-87 0.0015535362840752062 88-89 4.187429337129936E-6 90-91 0.0015493488547380762 92-93 0.005355722122189188 94-95 0.0 96-97 9.589213182027553E-4 98-99 7.118629873120891E-5 100-101 0.007461999078765547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.19405E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.82293354755415 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03900211426503 28.00372256895067 2 13.233177192701822 10.010351631651403 3 4.3799187839313 4.969841313249533 4 2.187544062083603 3.3095733477013916 5 1.2729219566902301 2.407282128955859 6 0.8446447010296898 1.9168164239003818 7 0.5966397192580356 1.579666511732963 8 0.4380771197585901 1.3255489431466458 9 0.34871821824034827 1.1870591395793562 >10 2.24101251684083 16.190765337226114 >50 0.21940828493196055 5.7927821980437 >100 0.17330192592255053 13.137870337481594 >500 0.017415560247134605 4.472334220748694 >1k 0.008017951258834878 5.090627544336382 >5k 1.5547220897966607E-4 0.4006914800576186 >10k+ 4.442063113704745E-5 0.2050668732375892 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13233 0.11082450483648087 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.374858674259871E-6 0.0 5 0.0 8.374858674259871E-6 0.0 3.3499434697039485E-5 0.0 6 1.6749717348519743E-5 8.374858674259871E-6 0.0 3.3499434697039485E-5 0.0 7 2.5124576022779617E-5 8.374858674259871E-6 0.0 3.3499434697039485E-5 1.6749717348519743E-5 8 2.5124576022779617E-5 8.374858674259871E-6 0.0 3.3499434697039485E-5 1.6749717348519743E-5 9 2.5124576022779617E-5 8.374858674259871E-6 0.0 7.537372806833885E-5 1.6749717348519743E-5 10-11 2.5124576022779617E-5 1.6749717348519743E-5 0.0 2.0099660818223694E-4 1.6749717348519743E-5 12-13 3.3499434697039485E-5 1.6749717348519743E-5 0.0 3.6849378166743435E-4 5.0249152045559234E-5 14-15 4.187429337129936E-5 2.093714668564968E-5 0.0 5.694903898496713E-4 6.699886939407897E-5 16-17 4.187429337129936E-5 2.5124576022779617E-5 0.0 7.997990033918178E-4 1.0049830409111847E-4 18-19 6.281144005694904E-5 2.5124576022779617E-5 0.0 0.0010929190569909132 1.0049830409111847E-4 20-21 7.537372806833885E-5 3.3499434697039485E-5 0.0 0.0016707843055148446 1.0887316276537834E-4 22-23 9.212344541685859E-5 3.3499434697039485E-5 0.0 0.003215945730915791 1.0887316276537834E-4 24-25 1.0049830409111847E-4 3.3499434697039485E-5 0.0 0.006398392027134542 1.172480214396382E-4 26-27 1.0049830409111847E-4 3.3499434697039485E-5 0.0 0.010912440852560613 1.2562288011389809E-4 28-29 1.2143545077676813E-4 3.3499434697039485E-5 0.0 0.027050793517859384 1.2562288011389809E-4 30-31 1.4237259746241782E-4 3.3499434697039485E-5 0.0 0.08281060257108161 1.2562288011389809E-4 32-33 1.4237259746241782E-4 3.7686864034169426E-5 0.0 0.19980319082115489 1.2562288011389809E-4 34-35 1.4656002679954778E-4 4.187429337129936E-5 0.0 0.3405761902767891 1.4237259746241782E-4 36-37 1.507474561366777E-4 4.6061722708429294E-5 0.0 0.5509191407395 1.4237259746241782E-4 38-39 1.6749717348519744E-4 5.443658138268917E-5 0.0 0.9047736694443282 1.4237259746241782E-4 40-41 1.7587203215945732E-4 6.699886939407897E-5 0.0 1.3249612662786316 1.4237259746241782E-4 42-43 1.9262174950797706E-4 7.537372806833885E-5 0.0 1.749407478748796 1.4237259746241782E-4 44-45 2.1774632553075667E-4 7.537372806833885E-5 0.0 2.186122859176751 1.5912231481093756E-4 46-47 2.5124576022779617E-4 7.537372806833885E-5 0.0 2.656471672040534 1.8424689083371717E-4 48-49 2.679954775763159E-4 7.537372806833885E-5 0.0 3.1493614170260877 1.9262174950797706E-4 50-51 2.679954775763159E-4 8.374858674259872E-5 0.0 3.674607428499644 2.093714668564968E-4 52-53 2.679954775763159E-4 8.374858674259872E-5 0.0 4.183204220928772 2.2612118420501655E-4 54-55 2.763703362505758E-4 8.793601607972866E-5 0.0 4.682885976299151 2.554331895649261E-4 56-57 3.26619488296135E-4 9.212344541685859E-5 0.0 5.211804363301368 2.6380804823918595E-4 58-59 3.26619488296135E-4 9.631087475398853E-5 0.0 5.7488589255056315 2.679954775763159E-4 60-61 3.349943469703949E-4 1.0049830409111847E-4 0.0 6.3173150203090325 2.679954775763159E-4 62-63 3.4336920564465473E-4 1.0049830409111847E-4 0.0 6.890289351367196 2.763703362505758E-4 64-65 3.4336920564465473E-4 1.0049830409111847E-4 0.0 7.442531719777229 2.763703362505758E-4 66-67 3.4336920564465473E-4 1.0887316276537834E-4 0.0 8.008982035928144 2.805577655877057E-4 68-69 3.5174406431891464E-4 1.0887316276537834E-4 0.0 8.60557346844772 2.8474519492483564E-4 70-71 3.768686403416942E-4 1.0887316276537834E-4 0.0 9.221309827896654 2.8893262426196557E-4 72-73 4.019932163644739E-4 1.0887316276537834E-4 0.0 9.825053389724047 3.0568234161048533E-4 74-75 4.1036807503873373E-4 1.0887316276537834E-4 0.0 10.419123989782673 3.140572002847452E-4 76-77 4.1036807503873373E-4 1.0887316276537834E-4 0.0 11.028214898873582 3.182446296218751E-4 78-79 4.1036807503873373E-4 1.0887316276537834E-4 0.0 11.648532306017337 3.182446296218751E-4 80-81 4.2711779238725344E-4 1.0887316276537834E-4 0.0 12.296499309074159 3.2661948829613497E-4 82-83 4.3549265106151335E-4 1.0887316276537834E-4 0.0 12.969163770361376 3.5174406431891464E-4 84-85 4.4805493907290313E-4 1.0887316276537834E-4 0.0 13.631518780620578 3.5174406431891464E-4 86-87 4.5224236841003306E-4 1.0887316276537834E-4 0.0 14.268200661613836 3.643063523303045E-4 88-89 4.5224236841003306E-4 1.0887316276537834E-4 0.0 14.90623089485365 3.7686864034169426E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 1035 0.0 51.864 2 GGTATCA 9410 0.0 50.745235 1 ATAACGC 1180 0.0 45.490116 3 GTATCAA 17415 0.0 42.971046 1 TAACGCA 1440 0.0 38.92587 4 TATCACG 980 0.0 36.83964 2 TCAACGC 20885 0.0 34.70874 4 ATCAACG 21100 0.0 34.265163 3 CAACGCA 22185 0.0 32.802933 5 TATCAAC 22215 0.0 32.8025 2 AACGCAG 23520 0.0 31.423248 6 GTATAAC 2360 0.0 29.998056 1 TACCTGG 10625 0.0 29.463503 2 ATCACGC 1290 0.0 28.354477 3 ACGCAGA 27200 0.0 27.136715 7 GTACATG 30875 0.0 26.623062 1 CGCAGAG 28135 0.0 26.234673 8 ACATGGG 32690 0.0 23.715149 3 TACATGG 34585 0.0 23.377586 2 GTACCTG 14615 0.0 21.944405 1 >>END_MODULE