FastQCFastQC Report
Fri 27 May 2016
SRR940190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122642
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10000.044.6319432
GGTATCA90650.043.7311971
ATAACGC11800.042.660273
GTATCAA161350.041.583151
TAACGCA13200.038.494524
ATCAACG197500.035.6353383
TCAACGC199850.035.1203884
CAACGCA211450.033.26045
TATCAAC207350.032.148522
AACGCAG226250.031.5470986
TACCTGG106650.028.6643052
ACGCAGA259750.027.4246377
GTATAAC23950.027.2528881
TATCACG10950.027.1731742
CGCAGAG271550.026.1460158
GTACATG313350.026.0680791
ATCACGC13750.025.9034943
ACATGGG341750.024.1513293
TACATGG349800.023.0215722
GTACCTG137800.022.4641321
Produced by FastQC (version 0.11.5)