FastQCFastQC Report
Fri 27 May 2016
SRR940190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122642
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135100.1114443534668433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.047.8719141
TATAACG8850.046.698452
GTATCAA182050.042.6702461
ATAACGC11300.038.6746943
TCAACGC216750.034.9119534
ATCAACG217700.034.6723253
CAACGCA226700.033.3778575
TATCAAC230300.033.0648082
TAACGCA12700.032.9151954
AACGCAG243300.031.6275336
TATCACG11550.030.4352022
TACCTGG108950.028.689642
ACGCAGA280850.027.36457
CGCAGAG289100.026.6489948
GTACATG313200.025.9553171
ATCACGC13850.025.3804573
GTATAAC24000.024.5475711
ACATGGG333050.023.3626423
TACATGG346400.023.216972
CCTATAA64150.022.515161
Produced by FastQC (version 0.11.5)