FastQCFastQC Report
Fri 27 May 2016
SRR940189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11338367
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG9650.046.2695732
GGTATCA85850.045.705181
GTATCAA158700.042.325111
ATAACGC11350.041.4303863
TATCACG8850.037.5707442
TCAACGC186200.036.0694774
ATCAACG187200.035.8775863
TAACGCA13350.035.2228134
TATCAAC195450.033.902642
CAACGCA198850.033.8699845
AACGCAG214250.032.3020326
GTATAAC23450.029.9808921
ACGCAGA245200.028.147527
CGCAGAG253750.027.2548988
TACCTGG102500.027.2488672
GTACATG295600.027.1104261
ATCACGC13600.024.7969993
ACATGGG313000.024.3714223
TACATGG327450.023.9060152
GCAGAGT301300.022.5591169
Produced by FastQC (version 0.11.5)