##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11338367 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17589375965692 34.0 31.0 34.0 30.0 34.0 2 31.809314956906935 34.0 31.0 34.0 30.0 34.0 3 32.197250715204405 34.0 31.0 34.0 30.0 34.0 4 35.70660219412549 37.0 35.0 37.0 35.0 37.0 5 35.77876099794618 37.0 35.0 37.0 35.0 37.0 6 35.8568348510857 37.0 37.0 37.0 35.0 37.0 7 35.84208166837429 37.0 37.0 37.0 35.0 37.0 8 35.843182796958324 37.0 37.0 37.0 35.0 37.0 9 37.60019560135952 39.0 39.0 39.0 35.0 39.0 10-11 37.53890644040716 39.0 38.0 39.0 35.0 39.0 12-13 37.45621437372772 39.0 38.0 39.0 35.0 39.0 14-15 38.89371198692017 41.0 39.0 41.0 36.0 41.0 16-17 38.826330458345545 41.0 39.0 41.0 36.0 41.0 18-19 38.7531909577455 41.0 39.0 41.0 35.5 41.0 20-21 38.67598058873911 41.0 39.0 41.0 35.0 41.0 22-23 38.56026851132972 40.5 39.0 41.0 35.0 41.0 24-25 38.449036576431155 40.0 38.5 41.0 34.0 41.0 26-27 38.29074244112931 40.0 38.0 41.0 34.0 41.0 28-29 38.12427208433101 40.0 38.0 41.0 34.0 41.0 30-31 37.96437529319698 40.0 38.0 41.0 33.5 41.0 32-33 38.025428661817 40.0 38.0 41.0 34.0 41.0 34-35 38.088607601076944 40.0 38.0 41.0 34.0 41.0 36-37 38.2010146170079 40.5 38.0 41.0 34.0 41.0 38-39 38.12203688591134 40.5 38.0 41.0 34.0 41.0 40-41 37.989847171113794 40.0 38.0 41.0 33.0 41.0 42-43 37.856871981653086 40.0 38.0 41.0 33.0 41.0 44-45 37.71745582939765 40.0 38.0 41.0 33.0 41.0 46-47 37.50390131136168 40.0 38.0 41.0 33.0 41.0 48-49 37.31652670971049 40.0 37.5 41.0 32.0 41.0 50-51 36.39358630744621 39.0 36.0 40.5 31.0 40.5 52-53 36.38814963389349 39.0 36.0 40.5 30.5 41.0 54-55 36.77237136529449 40.0 36.5 41.0 31.0 41.0 56-57 36.65967391953356 40.0 36.0 41.0 31.0 41.0 58-59 36.40722473527272 39.0 35.5 41.0 31.0 41.0 60-61 36.10655335111308 39.0 35.0 41.0 30.5 41.0 62-63 35.76994451670157 38.5 35.0 41.0 30.0 41.0 64-65 35.38659548592844 37.5 35.0 40.0 29.5 41.0 66-67 34.98548984170296 37.0 35.0 40.0 29.0 41.0 68-69 34.543081292041435 36.5 35.0 39.0 29.0 41.0 70-71 34.110372684179296 36.0 34.0 39.0 28.0 41.0 72-73 33.676844205166404 35.5 34.0 38.5 27.5 40.0 74-75 33.225207298370215 35.0 34.0 37.0 26.0 39.0 76-77 32.689210448030124 35.0 33.5 37.0 25.5 39.0 78-79 32.2274591217589 35.0 33.0 36.0 25.0 38.5 80-81 31.82883381707436 35.0 33.0 36.0 23.5 37.0 82-83 31.403929948642514 35.0 33.0 35.0 21.0 37.0 84-85 31.041176740883408 35.0 32.0 35.0 19.5 36.5 86-87 30.66463843514679 35.0 32.0 35.0 16.5 36.0 88-89 30.283034408746868 35.0 31.5 35.0 8.5 36.0 90-91 29.89698494501016 34.0 31.0 35.0 2.0 35.0 92-93 29.501826100707447 34.0 31.0 35.0 2.0 35.0 94-95 29.044145025469717 34.0 30.0 35.0 2.0 35.0 96-97 28.550774022396702 34.0 30.0 35.0 2.0 35.0 98-99 28.012150338756896 34.0 29.0 35.0 2.0 35.0 100-101 26.08063731752553 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1748634919212151 1101 2 0.08881687681611083 1101 3 0.14716403642584197 1101 4 0.14922611420490028 1101 5 0.24998349536300424 1101 6 0.3098131349114297 1101 7 0.314249698137246 1101 8 0.30568904054600665 1101 9 0.4547076667555885 1101 10-11 0.4958377741588009 1101 12-13 0.5060190415213626 1101 14-15 0.5767688110402815 1101 16-17 0.6193135996309422 1101 18-19 0.6348564739652502 1101 20-21 0.6433335517429057 1101 22-23 0.6575398152845509 1101 24-25 0.6307676796703703 1101 26-27 0.7058990054360592 1101 28-29 0.7044200587076048 1101 30-31 0.7239785695201704 1101 32-33 0.7261846970354568 1101 34-35 0.6939904721626533 1101 36-37 0.7501882701804092 1101 38-39 0.7690003501818623 1101 40-41 0.8111987178415845 1101 42-43 0.8193076967811947 1101 44-45 0.8230397933465028 1101 46-47 0.8842969596305039 1101 48-49 0.8275056503690408 1101 50-51 0.763895392571655 1101 52-53 0.9038211821214546 1101 54-55 0.9787348945249619 1101 56-57 0.8896905416337191 1101 58-59 0.8757371275550838 1101 60-61 0.9471774911157596 1101 62-63 0.9760215208468921 1101 64-65 0.9713868645973776 1101 66-67 0.990368483800026 1101 68-69 0.9490819525016363 1101 70-71 0.9073025298272555 1101 72-73 0.8995027725212665 1101 74-75 0.8809726148730022 1101 76-77 1.0270675623502399 1101 78-79 1.0442237376197454 1101 80-81 1.0146767561813164 1101 82-83 0.937583537515426 1101 84-85 0.9669755469466956 1101 86-87 0.9262529964614998 1101 88-89 0.938277207137336 1101 90-91 1.0042253039522642 1101 92-93 0.9637515487975747 1101 94-95 1.0673484253162613 1101 96-97 1.0131665085661474 1101 98-99 0.9625537313878887 1101 100-101 1.1198112333936798 1102 1 0.23925126973465893 1102 2 0.6887933582173176 1102 3 0.3601491127569645 1102 4 0.272596696479134 1102 5 0.2323257863889836 1102 6 0.18172661325061767 1102 7 0.2041020325435028 1102 8 0.17754820012854822 1102 9 0.202673609863659 1102 10-11 0.19060319407071802 1102 12-13 0.21744422616239234 1102 14-15 0.21613098233662953 1102 16-17 0.27281099282682675 1102 18-19 0.2763387985186867 1102 20-21 0.29125489081802414 1102 22-23 0.27154028051518964 1102 24-25 0.2873393913395219 1102 26-27 0.280727779694395 1102 28-29 0.2760484622162167 1102 30-31 0.26473523111545916 1102 32-33 0.2533418633898492 1102 34-35 0.24260223814577841 1102 36-37 0.3335778143996535 1102 38-39 0.37386654196892266 1102 40-41 0.32622731171094443 1102 42-43 0.20194161484523931 1102 44-45 0.2602771064703404 1102 46-47 0.35632795922415994 1102 48-49 0.3272856611981183 1102 50-51 0.292246543306085 1102 52-53 0.4268995873548036 1102 54-55 0.47674368105911924 1102 56-57 0.43452827197057076 1102 58-59 0.39234397244776886 1102 60-61 0.39569096800819636 1102 62-63 0.5214854533060631 1102 64-65 0.5147358628479495 1102 66-67 0.42699778674258937 1102 68-69 0.424986837166351 1102 70-71 0.4422147359262212 1102 72-73 0.4455146572452193 1102 74-75 0.4741882762989249 1102 76-77 0.5417697833512918 1102 78-79 0.5650480579313495 1102 80-81 0.48159002737270384 1102 82-83 0.3623618101562478 1102 84-85 0.23819945925185593 1102 86-87 0.3381835440186016 1102 88-89 0.2762211064911213 1102 90-91 0.3219823183265298 1102 92-93 0.31014224198466067 1102 94-95 0.25558479165807313 1102 96-97 0.13963050995502613 1102 98-99 0.20400491502085671 1102 100-101 0.4040623151804752 1103 1 0.17193146113429947 1103 2 0.6205660541737004 1103 3 0.264340054434669 1103 4 0.19056018550659815 1103 5 0.12093834789082791 1103 6 0.12923362244733028 1103 7 0.1525063676357803 1103 8 0.07245705118343437 1103 9 0.051181357227378044 1103 10-11 0.04377821707112162 1103 12-13 0.12148477061810325 1103 14-15 0.07848048191560508 1103 16-17 0.06339918852981441 1103 18-19 0.04397492652573476 1103 20-21 0.10764746630463407 1103 22-23 0.05666544714405575 1103 24-25 -0.0078099968555775945 1103 26-27 0.06043790821943418 1103 28-29 -0.03632324634269679 1103 30-31 0.06707228885426986 1103 32-33 0.09338545794441444 1103 34-35 0.014941754618782 1103 36-37 0.03187521888504108 1103 38-39 0.08718256410769953 1103 40-41 0.03053977128221419 1103 42-43 0.09069976217016773 1103 44-45 0.11693078454697314 1103 46-47 0.10075519745002026 1103 48-49 0.02981250401994373 1103 50-51 -0.03585741451238533 1103 52-53 0.13852525024118023 1103 54-55 0.2508530952226451 1103 56-57 0.22405513048411052 1103 58-59 0.21975800026716996 1103 60-61 0.16936937924491247 1103 62-63 0.02378512428838775 1103 64-65 0.13762311881365719 1103 66-67 0.1327233076981713 1103 68-69 0.12474787739953541 1103 70-71 0.1136918583531994 1103 72-73 0.08870799855345268 1103 74-75 0.18905127253525933 1103 76-77 0.2505523058791681 1103 78-79 0.23573037006439534 1103 80-81 0.14199186994165558 1103 82-83 0.09449342280211326 1103 84-85 0.004312301099698601 1103 86-87 0.04761039760592922 1103 88-89 -0.010691399149287406 1103 90-91 -0.033336715966665764 1103 92-93 0.026681669020316434 1103 94-95 -0.01436135457269927 1103 96-97 -0.19042114861345638 1103 98-99 -0.20811882059955522 1103 100-101 0.05774000908135335 1104 1 0.20344147868453888 1104 2 0.7099079842457883 1104 3 0.34071567734658714 1104 4 0.18220389023228734 1104 5 0.15594939279655762 1104 6 0.12842380466689463 1104 7 0.0886377459904466 1104 8 -1.0807716641636489E-5 1104 9 0.1030832585703294 1104 10-11 0.0560479776461591 1104 12-13 0.11228639497567627 1104 14-15 0.08263728325643882 1104 16-17 0.124225283825389 1104 18-19 0.13041434719563938 1104 20-21 0.1193776082710869 1104 22-23 0.09088062565862032 1104 24-25 0.0907665036591041 1104 26-27 0.05537410727544767 1104 28-29 0.0506753869242047 1104 30-31 0.07688187620201603 1104 32-33 0.015454204823591056 1104 34-35 0.10200017596073252 1104 36-37 0.1301251110049222 1104 38-39 0.10968971830298102 1104 40-41 0.08946431905010854 1104 42-43 -0.02816378549574239 1104 44-45 0.044407312927532416 1104 46-47 0.04083663834950357 1104 48-49 0.057602556337315036 1104 50-51 -0.03963649708042993 1104 52-53 0.10653354923849889 1104 54-55 0.18477285977684943 1104 56-57 0.14141709448288253 1104 58-59 0.11282840726105547 1104 60-61 0.1489427511270094 1104 62-63 0.10723936686729729 1104 64-65 0.1080968647304914 1104 66-67 0.18537587594938998 1104 68-69 0.16317191505299888 1104 70-71 0.04210064807722347 1104 72-73 -0.046439235052069705 1104 74-75 0.02948048870295139 1104 76-77 0.12447868191625844 1104 78-79 0.10411745766460001 1104 80-81 -0.016519010750187135 1104 82-83 -0.20679269742015904 1104 84-85 -0.08754168392851724 1104 86-87 -0.01775108976509543 1104 88-89 -0.0892896024585248 1104 90-91 -0.17149561350373332 1104 92-93 -0.17713539251593957 1104 94-95 -0.23302877287046186 1104 96-97 -0.31588974844632745 1104 98-99 -0.2722036720100469 1104 100-101 -0.10179560282909605 1105 1 0.018650359853587872 1105 2 0.5292707031971986 1105 3 0.1674185615227941 1105 4 0.15800222679945364 1105 5 0.0549455084307553 1105 6 0.09695291176678467 1105 7 0.09267471520544746 1105 8 0.024743346109701747 1105 9 0.1026512292970807 1105 10-11 0.08761804331177103 1105 12-13 0.008765973082127232 1105 14-15 -0.1576127532373377 1105 16-17 -0.0799102182077931 1105 18-19 -0.1995440954340637 1105 20-21 -0.1257558593398187 1105 22-23 -0.13942774153738213 1105 24-25 -0.0746259089088781 1105 26-27 -0.05943600357486645 1105 28-29 0.09498076270592293 1105 30-31 0.018891509247374927 1105 32-33 0.09137041526273748 1105 34-35 0.05229263798695172 1105 36-37 0.0450435659277133 1105 38-39 -0.18719165518901804 1105 40-41 -0.16909819894411982 1105 42-43 -0.07196869043946919 1105 44-45 -0.08277603057555183 1105 46-47 -0.015142143072118586 1105 48-49 -0.18796562924087823 1105 50-51 -0.20605563990462628 1105 52-53 0.04611958966722085 1105 54-55 -0.07584698205898377 1105 56-57 0.10154190083780179 1105 58-59 0.006739195645486973 1105 60-61 -0.03503336924264033 1105 62-63 0.021148501304459444 1105 64-65 0.06394664757020507 1105 66-67 0.005017749989121967 1105 68-69 -0.055766422720232356 1105 70-71 -0.12398313260521832 1105 72-73 -0.20129756940328747 1105 74-75 -0.2130973733210837 1105 76-77 -0.14726082425584508 1105 78-79 -0.09518169018643619 1105 80-81 -0.1225046637872893 1105 82-83 -0.25185342842061686 1105 84-85 -0.3250042655071148 1105 86-87 -0.21599172737778716 1105 88-89 -0.28462312110961463 1105 90-91 -0.4267123206411618 1105 92-93 -0.3946637676961089 1105 94-95 -0.4085448926704913 1105 96-97 -0.4142178445087801 1105 98-99 -0.49091880241338615 1105 100-101 -0.4200953837779515 1106 1 -0.0026421690716489366 1106 2 0.5325520022414523 1106 3 0.08221702952051402 1106 4 0.009011349027254312 1106 5 -0.022030121177841977 1106 6 -0.02562783423865511 1106 7 0.0020895541604915024 1106 8 -0.07488534368960842 1106 9 -0.04087486863012657 1106 10-11 -0.15136237055702395 1106 12-13 -0.10418280881943787 1106 14-15 -0.05527121391013878 1106 16-17 -0.015181486741944639 1106 18-19 -0.07839922238890296 1106 20-21 -0.09155942883833035 1106 22-23 -0.1640707302818285 1106 24-25 -0.14207702817737555 1106 26-27 -0.3006055932312961 1106 28-29 -0.2051672856018243 1106 30-31 -0.13992938343677253 1106 32-33 -0.1614754694535847 1106 34-35 -0.2288004185554371 1106 36-37 -0.18697662049265773 1106 38-39 -0.1926399243577066 1106 40-41 -0.3094033087322785 1106 42-43 -0.2771576179300794 1106 44-45 -0.186199709446079 1106 46-47 -0.21004594999452308 1106 48-49 -0.19754072814590273 1106 50-51 -0.27416273183978745 1106 52-53 -0.10006315522488052 1106 54-55 -0.08332403969198765 1106 56-57 -0.10703375474022891 1106 58-59 -0.10701650000404328 1106 60-61 -0.16317189971425705 1106 62-63 -0.2077682669810983 1106 64-65 -0.2433135742649597 1106 66-67 -0.16337456765246827 1106 68-69 -0.37295566830550086 1106 70-71 -0.23059616611615752 1106 72-73 -0.2410920947177786 1106 74-75 -0.14571075797011446 1106 76-77 -0.07660966718470519 1106 78-79 -0.06805326853375959 1106 80-81 -0.1216290567907734 1106 82-83 -0.1375741547448861 1106 84-85 -0.20065928471442263 1106 86-87 -0.22316897910713607 1106 88-89 -0.2710063963931084 1106 90-91 -0.19544320249283587 1106 92-93 -0.20723826268955747 1106 94-95 -0.32969852428890434 1106 96-97 -0.3323858157434323 1106 98-99 -0.39041354847690357 1106 100-101 -0.3900801596459438 1107 1 0.14665619602408242 1107 2 0.6084767510302527 1107 3 0.21021616453447933 1107 4 0.12091973298495873 1107 5 0.11669553289169698 1107 6 0.053520054405424844 1107 7 0.061292917520930246 1107 8 0.008217420610357351 1107 9 0.02716997518414388 1107 10-11 0.0014039008165696032 1107 12-13 0.018582827443545114 1107 14-15 0.01355191211886364 1107 16-17 0.024402218061744918 1107 18-19 0.04671308392953222 1107 20-21 -0.007290923379869696 1107 22-23 -0.021450468849074866 1107 24-25 0.03488585616464235 1107 26-27 0.08676037211652243 1107 28-29 0.018840676920447663 1107 30-31 -0.005315999046324293 1107 32-33 -1.5912484776947622E-5 1107 34-35 -0.030458103719858798 1107 36-37 -0.009260130435656322 1107 38-39 -0.030110725858996545 1107 40-41 0.08793452597787876 1107 42-43 0.10166945619586443 1107 44-45 0.017492439785947056 1107 46-47 -0.007560700406209264 1107 48-49 0.07903982704688417 1107 50-51 -0.12458203140531765 1107 52-53 0.024442442414915888 1107 54-55 0.07394813121703692 1107 56-57 0.11982068600290319 1107 58-59 0.14485226397776074 1107 60-61 0.07402317783387247 1107 62-63 0.059901408230693676 1107 64-65 -0.012367675477769069 1107 66-67 0.0020105513498975824 1107 68-69 0.10739148750015914 1107 70-71 0.0768312543703118 1107 72-73 0.022740163117738632 1107 74-75 0.0016442165748387083 1107 76-77 0.16623137705783364 1107 78-79 0.12496979474785519 1107 80-81 0.06683969425141356 1107 82-83 0.019731309059753954 1107 84-85 -0.02147705991152904 1107 86-87 0.04776147638143513 1107 88-89 0.07549556425526305 1107 90-91 0.058054718159898044 1107 92-93 -0.015588713691020217 1107 94-95 -0.002448988544607289 1107 96-97 -0.03330575937203761 1107 98-99 -0.07895391213714475 1107 100-101 0.12333029879665602 1108 1 0.0586754339992126 1108 2 0.19403321081867375 1108 3 0.09159143698243355 1108 4 0.04894962916268497 1108 5 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-0.9685993893551768 2316 88-89 -0.9567773566250786 2316 90-91 -0.8685535491699561 2316 92-93 -0.8827622621685656 2316 94-95 -0.8188985008684639 2316 96-97 -0.6801777314039619 2316 98-99 -0.7182832754143931 2316 100-101 -1.1093086998588184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77608.0 3 23749.0 4 18685.0 5 14938.0 6 13909.0 7 13475.0 8 14544.0 9 15925.0 10 18037.0 11 21936.0 12 25687.0 13 29498.0 14 30248.0 15 33752.0 16 39497.0 17 48821.0 18 45459.0 19 38821.0 20 40258.0 21 43674.0 22 45188.0 23 50969.0 24 58084.0 25 67215.0 26 78701.0 27 93941.0 28 115286.0 29 141355.0 30 173765.0 31 216379.0 32 272453.0 33 358170.0 34 501531.0 35 769683.0 36 1404542.0 37 2847150.0 38 3069399.0 39 465990.0 40 45.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.97409661486695 18.298615660072493 13.94064669578884 24.78664102927171 2 16.71869605698597 20.600365315931757 38.00511174092019 24.675826886162085 3 18.14515524280028 25.575657386876028 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25.41922031750491 26.535755993652742 23.697087202528564 24-25 24.311225246993644 25.737917898112066 26.229821264495794 23.72103559039849 26-27 24.14242296300753 25.983873190246214 25.90220611807449 23.97149772867177 28-29 24.072460843376817 25.84328969670503 26.006314463827472 24.077934996090676 30-31 24.133040932512603 25.525366279931188 26.157274230574284 24.184318556981925 32-33 23.906263833471677 25.79896838887264 26.179052362718913 24.115715414936766 34-35 24.525426084041175 25.603624388748337 25.887633361492636 23.983316165717852 36-37 24.313712031688 25.37314295325677 25.966273935378194 24.34687107967704 38-39 24.227441460000268 25.451912227193052 26.203655893272238 24.11699041953444 40-41 24.36205593328448 24.92170721537376 26.638331042966712 24.077905808375043 42-43 23.854848632546105 25.279649793772567 26.50482380815929 24.360677765522034 44-45 23.779074226957 25.484956577303596 26.23312381633659 24.502845379402814 46-47 23.988531622500638 25.3392570297952 26.22108096139953 24.451130386304634 48-49 23.92545681978973 25.492061559741636 26.08487753154116 24.497604088927467 50-51 23.437237002521247 25.72107843742667 26.22607329436486 24.61561126568722 52-53 23.913475765074878 25.310463537621793 26.02632167943957 24.749739017863767 54-55 23.484710036769524 25.275612630944867 26.36094812500659 24.878729207279022 56-57 23.499142412465503 25.630243686138048 26.330659038882626 24.53995486251382 58-59 23.871839383611707 25.32222731268019 26.058698361957678 24.747234941750428 60-61 23.73742457657279 25.09424344371763 26.50250912968198 24.665822850027606 62-63 23.90724160130745 25.126466628664406 26.30354567104622 24.662746098981923 64-65 23.725338850671736 25.174204807897425 25.998342822287274 25.10211351914356 66-67 23.55324075350377 25.78982379373635 25.713682807023797 24.943252645736084 68-69 23.57557211840119 25.18944168213231 26.173274712769164 25.061711486697348 70-71 23.615508882020674 25.281793455616935 25.900246049703917 25.20245161265847 72-73 23.739320655508184 25.5051719284819 25.62120875717495 25.134298658834965 74-75 23.90237755102536 25.509338323217662 25.690702716116714 24.897581409640264 76-77 24.102891341786375 25.395694866674067 25.844670518541996 24.65674327299756 78-79 23.858601475253824 25.585982772899353 25.832225992388846 24.723189759457977 80-81 24.105051547642283 25.61446179056517 25.824926584997648 24.455560076794907 82-83 24.14511088709233 25.56236639704133 25.83084488104876 24.461677834817582 84-85 24.10419161063867 25.592749054202212 25.73886647533692 24.5641928598222 86-87 24.418659528607954 25.519555238062807 25.71960742558999 24.342177807739244 88-89 24.25446002489669 25.358570307833446 25.730834148534846 24.65613551873502 90-91 24.47423284543915 25.453186633539843 25.875856632531114 24.196723888489892 92-93 24.796289430819137 25.23957541667189 25.748865073885113 24.215270078623863 94-95 24.76118888770669 25.13155070991422 25.607123091588523 24.500137310790564 96-97 25.175698519620976 25.02841620514148 25.34886149371833 24.447023781519214 98-99 24.869486388566582 24.853840555499897 25.5545576157251 24.722115440208423 100-101 24.969767286668265 24.69062003808351 25.703888182919478 24.635724492328748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1189.0 1 808.0 2 1145.5 3 1574.5 4 973.0 5 662.0 6 850.0 7 1510.0 8 2551.5 9 3099.5 10 2883.5 11 2819.0 12 2959.5 13 3159.0 14 3458.5 15 3708.0 16 3953.5 17 4206.0 18 4564.0 19 5030.5 20 5663.0 21 6551.5 22 7882.5 23 9605.0 24 11556.0 25 14395.0 26 18254.0 27 23321.5 28 29390.0 29 37227.5 30 49213.5 31 66620.0 32 86861.5 33 105720.0 34 131629.5 35 158535.0 36 181615.0 37 206554.5 38 228397.5 39 237443.0 40 247589.5 41 280376.0 42 318069.0 43 352682.5 44 383297.0 45 405914.0 46 421993.0 47 445115.5 48 482921.0 49 507599.5 50 521963.5 51 532965.0 52 525207.0 53 520973.5 54 520693.5 55 495184.5 56 463017.0 57 425830.5 58 381489.5 59 331912.5 60 269517.0 61 207746.0 62 160090.5 63 123995.0 64 92877.0 65 69743.0 66 51268.0 67 39054.0 68 27747.0 69 18229.5 70 13114.5 71 9180.5 72 6533.5 73 4694.5 74 3500.5 75 2681.5 76 1884.0 77 1200.5 78 764.5 79 533.5 80 386.5 81 274.5 82 233.5 83 206.5 84 179.5 85 152.0 86 113.5 87 106.5 88 98.0 89 84.0 90 75.0 91 64.5 92 59.0 93 53.0 94 46.5 95 38.0 96 29.0 97 25.5 98 21.5 99 15.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029651536239742458 2 0.035878182457844236 3 0.030101336462296555 4 0.025488679278065352 5 0.026688146538209605 6 0.028531445489460697 7 0.029237014466016138 8 0.027481911636834475 9 0.031291984110233866 10-11 0.017872944137370048 12-13 0.02638386991707007 14-15 0.022653173953533168 16-17 0.036619029883227454 18-19 0.04259431715343135 20-21 0.04617507970945022 22-23 0.03547689010242833 24-25 0.014001134378522056 26-27 0.023892329468608663 28-29 0.02892391823266966 30-31 0.026577901385622812 32-33 0.033219069377450915 34-35 0.03564887254046372 36-37 0.03177265297551226 38-39 0.018719626909236578 40-41 0.015469599810978072 42-43 0.015279978148528795 44-45 0.02233566791408322 46-47 0.017436373333126365 48-49 0.02105241433797301 50-51 0.023874690244194776 52-53 0.029104720282911993 54-55 0.029109130089015466 56-57 0.032315059126239255 58-59 0.03028654831864236 60-61 0.029642716627535516 62-63 0.03487274666625273 64-65 0.030277728706435417 66-67 0.024840437780855037 68-69 0.024293621824024572 70-71 0.03411866982255911 72-73 0.016580870949052893 74-75 0.02558128520623825 76-77 0.0442127159934054 78-79 0.026719015180933902 80-81 0.026278034570586754 82-83 0.02652939351848463 84-85 0.02613251096917219 86-87 0.02050559838114254 88-89 0.024024623651712807 90-91 0.020607023921522385 92-93 0.036592571046606626 94-95 0.07258981826924459 96-97 0.08329241768236996 98-99 0.08353936682416435 100-101 0.08448306533030726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1338367E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.012854981353875 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47120713396191 30.19818466318938 2 12.629059921673743 10.106494863935195 3 4.14427140150259 4.974723917750859 4 2.0046099061530414 3.208406618763481 5 1.1738014056206951 2.3483572710005105 6 0.7905028388981987 1.8978165273109298 7 0.5594590117485737 1.5669886613575064 8 0.4268079679382918 1.3662244260800962 9 0.3315878475218702 1.1940998810824686 >10 2.0904086070247923 15.927616876657064 >50 0.19917747436632677 5.576149823973306 >100 0.15713176148657026 12.544672368006962 >500 0.015180768209264848 4.15167952919611 >1k 0.006660739376573344 4.509424901463401 >5k 8.880967822179895E-5 0.24751216304453671 >10k+ 4.4404839110899475E-5 0.18164750718824316 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.819612206943028E-6 0.0 0.0 0.0 0.0 3 8.819612206943028E-6 0.0 0.0 0.0 0.0 4 8.819612206943028E-6 0.0 0.0 1.7639224413886056E-5 0.0 5 8.819612206943028E-6 8.819612206943028E-6 0.0 1.7639224413886056E-5 0.0 6 8.819612206943028E-6 8.819612206943028E-6 0.0 1.7639224413886056E-5 0.0 7 8.819612206943028E-6 8.819612206943028E-6 0.0 1.7639224413886056E-5 0.0 8 3.527844882777211E-5 8.819612206943028E-6 0.0 3.527844882777211E-5 0.0 9 3.527844882777211E-5 1.7639224413886056E-5 0.0 7.055689765554422E-5 8.819612206943028E-6 10-11 3.527844882777211E-5 1.7639224413886056E-5 0.0 2.557687540013478E-4 8.819612206943028E-6 12-13 3.527844882777211E-5 1.7639224413886056E-5 0.0 4.409806103471514E-4 3.527844882777211E-5 14-15 3.527844882777211E-5 1.7639224413886056E-5 0.0 6.570611094172557E-4 5.7327479345129685E-5 16-17 3.527844882777211E-5 1.7639224413886056E-5 0.0 8.819612206943028E-4 6.17372854486012E-5 18-19 5.7327479345129685E-5 1.7639224413886056E-5 0.0 0.001133320168592179 6.17372854486012E-5 20-21 7.055689765554422E-5 1.7639224413886056E-5 0.0 0.001569890972835859 6.17372854486012E-5 22-23 9.260592817290179E-5 2.204903051735757E-5 0.0 0.003104503496843946 7.055689765554422E-5 24-25 1.3229418310414543E-4 2.6458836620829085E-5 0.0 0.006169318738756648 7.055689765554422E-5 26-27 1.3229418310414543E-4 2.6458836620829085E-5 0.0 0.010768746504677438 7.055689765554422E-5 28-29 1.7198243803538904E-4 2.6458836620829085E-5 0.0 0.0258061853175153 7.055689765554422E-5 30-31 1.8521185634580358E-4 4.409806103471514E-5 0.0 0.08063330460197664 7.055689765554422E-5 32-33 1.896216624492751E-4 5.291767324165817E-5 0.0 0.19608643819696434 7.937650986248725E-5 34-35 2.0726088686316116E-4 5.291767324165817E-5 0.0 0.3358640622587009 8.819612206943028E-5 36-37 2.469491417944048E-4 5.291767324165817E-5 0.0 0.5465116802093282 8.819612206943028E-5 38-39 2.6458836620829086E-4 5.291767324165817E-5 0.0 0.8996577725875339 8.819612206943028E-5 40-41 2.6458836620829086E-4 5.7327479345129685E-5 0.0 1.3181086835520492 8.819612206943028E-5 42-43 2.866373967256484E-4 6.17372854486012E-5 0.0 1.7345134444845542 9.260592817290179E-5 44-45 3.17506039449949E-4 6.17372854486012E-5 0.0 2.1679488765886656 1.0583534648331634E-4 46-47 3.4837468217424963E-4 6.17372854486012E-5 0.0 2.635921910095166 1.0583534648331634E-4 48-49 3.792433248985502E-4 6.17372854486012E-5 0.0 3.122883568683215 1.1465495869025937E-4 50-51 3.792433248985502E-4 6.17372854486012E-5 0.0 3.642737970997058 1.1465495869025937E-4 52-53 3.792433248985502E-4 6.17372854486012E-5 0.0 4.152004428856466 1.3670398920761692E-4 54-55 3.792433248985502E-4 6.614709155207271E-5 0.0 4.651128332677889 1.54343213621503E-4 56-57 4.057021615193793E-4 7.937650986248725E-5 0.0 5.175815882481137 1.63162825828446E-4 58-59 4.057021615193793E-4 8.819612206943028E-5 0.0 5.713089018903692 1.7639224413886054E-4 60-61 4.057021615193793E-4 8.819612206943028E-5 0.0 6.279506563864091 2.0726088686316116E-4 62-63 4.057021615193793E-4 8.819612206943028E-5 0.0 6.847762997969637 2.204903051735757E-4 64-65 4.145217737263223E-4 8.819612206943028E-5 0.0 7.39868889408854 2.204903051735757E-4 66-67 4.4980022255409444E-4 8.819612206943028E-5 0.0 7.959638279480634 2.2490011127704722E-4 68-69 4.4980022255409444E-4 8.819612206943028E-5 0.0 8.54890302986312 2.513589478978763E-4 70-71 4.6743944696798047E-4 9.260592817290179E-5 0.0 9.15974496150989 2.557687540013478E-4 72-73 4.762590591749235E-4 9.701573427637331E-5 0.0 9.754579297001058 2.6899817231176235E-4 74-75 4.762590591749235E-4 9.701573427637331E-5 0.0 10.343804359128612 2.7340797841523385E-4 76-77 4.762590591749235E-4 1.0583534648331634E-4 0.0 10.94013802869496 2.7340797841523385E-4 78-79 4.762590591749235E-4 1.0583534648331634E-4 0.0 11.551024058402767 2.778177845187054E-4 80-81 5.203571202096386E-4 1.0583534648331634E-4 0.0 12.18899070739199 2.9104720282911993E-4 82-83 5.379963446235247E-4 1.1465495869025937E-4 0.0 12.856339894448645 3.0868642724300597E-4 84-85 5.468159568304677E-4 1.1465495869025937E-4 0.0 13.50967912751457 3.0868642724300597E-4 86-87 5.600453751408822E-4 1.1465495869025937E-4 0.0 14.137728122577087 3.1309623334647746E-4 88-89 5.644551812443538E-4 1.1465495869025937E-4 0.0 14.77216251687743 3.17506039449949E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 965 0.0 46.269573 2 GGTATCA 8585 0.0 45.70518 1 GTATCAA 15870 0.0 42.32511 1 ATAACGC 1135 0.0 41.430386 3 TATCACG 885 0.0 37.570744 2 TCAACGC 18620 0.0 36.069477 4 ATCAACG 18720 0.0 35.877586 3 TAACGCA 1335 0.0 35.222813 4 TATCAAC 19545 0.0 33.90264 2 CAACGCA 19885 0.0 33.869984 5 AACGCAG 21425 0.0 32.302032 6 GTATAAC 2345 0.0 29.980892 1 ACGCAGA 24520 0.0 28.14752 7 CGCAGAG 25375 0.0 27.254898 8 TACCTGG 10250 0.0 27.248867 2 GTACATG 29560 0.0 27.110426 1 ATCACGC 1360 0.0 24.796999 3 ACATGGG 31300 0.0 24.371422 3 TACATGG 32745 0.0 23.906015 2 GCAGAGT 30130 0.0 22.559116 9 >>END_MODULE