FastQCFastQC Report
Fri 27 May 2016
SRR940189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11338367
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124730.11000702305720038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10900.050.987782
GGTATCA94550.048.041661
ATAACGC11900.045.5033723
GTATCAA169600.042.9194951
TAACGCA12850.042.139314
TATCACG10450.036.3647352
TCAACGC203300.035.092824
ATCAACG204350.034.842773
CAACGCA213150.033.626675
TATCAAC218800.032.825492
AACGCAG228250.031.8807076
GTATAAC22500.031.0424861
TACCTGG106650.029.7078842
ATCACGC13400.028.3576933
ACGCAGA261200.027.895377
CGCAGAG270950.026.8740378
GTACATG302500.026.7335011
ACATGGG317750.024.0373273
TACATGG333750.023.8966062
GTACCTG139750.023.4595131
Produced by FastQC (version 0.11.5)