FastQCFastQC Report
Fri 27 May 2016
SRR940188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11509367
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93000.046.9866681
TATAACG8900.046.430642
GTATCAA165850.042.0989881
ATAACGC11400.038.3318983
TATCACG10900.037.0397342
TAACGCA12300.036.299614
TCAACGC195000.034.9296154
ATCAACG196400.034.6321033
CAACGCA205350.033.308755
TATCAAC210150.032.682432
AACGCAG220200.031.4713446
GTATAAC21400.028.6316071
TACCTGG106000.028.0955242
ATCACGC14150.027.525473
ACGCAGA254300.027.2515957
GTACATG304750.026.4956741
CGCAGAG262900.026.4142278
ACATGGG325300.023.7856123
TACATGG336300.023.6430822
TATAAGC58250.022.4240533
Produced by FastQC (version 0.11.5)