##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11509367 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3545049871118 34.0 31.0 34.0 31.0 34.0 2 32.361384079593606 34.0 31.0 34.0 31.0 34.0 3 32.46343295856323 34.0 31.0 34.0 31.0 34.0 4 35.85055476986702 37.0 37.0 37.0 35.0 37.0 5 35.85794084070827 37.0 37.0 37.0 35.0 37.0 6 35.92828823687697 37.0 37.0 37.0 35.0 37.0 7 35.89319794911397 37.0 37.0 37.0 35.0 37.0 8 35.880647823637915 37.0 37.0 37.0 35.0 37.0 9 37.64222098400373 39.0 39.0 39.0 35.0 39.0 10-11 37.57007248965125 39.0 38.0 39.0 35.0 39.0 12-13 37.51189031507988 39.0 38.0 39.0 35.0 39.0 14-15 38.96624471180735 41.0 39.0 41.0 36.0 41.0 16-17 38.87931386669658 41.0 39.0 41.0 36.0 41.0 18-19 38.83414630882828 41.0 39.0 41.0 36.0 41.0 20-21 38.764564723672464 41.0 39.0 41.0 35.5 41.0 22-23 38.6452616812028 40.5 39.0 41.0 35.0 41.0 24-25 38.52528705531764 40.0 39.0 41.0 35.0 41.0 26-27 38.36694659228435 40.0 38.0 41.0 34.0 41.0 28-29 38.20055990047064 40.0 38.0 41.0 34.0 41.0 30-31 38.15677217522041 40.0 38.0 41.0 34.0 41.0 32-33 38.16709589675957 40.0 38.0 41.0 34.0 41.0 34-35 38.18543765265284 40.0 38.0 41.0 34.0 41.0 36-37 38.30949486622505 41.0 39.0 41.0 34.0 41.0 38-39 38.23001490872608 41.0 38.0 41.0 34.0 41.0 40-41 38.10874194905767 40.0 38.0 41.0 34.0 41.0 42-43 37.992485468575296 40.0 38.0 41.0 33.5 41.0 44-45 37.835247455398715 40.0 38.0 41.0 33.0 41.0 46-47 37.649442840774825 40.0 38.0 41.0 33.0 41.0 48-49 37.45722640524018 40.0 38.0 41.0 33.0 41.0 50-51 36.51456578802292 39.0 36.0 40.5 31.0 40.5 52-53 36.59061823295755 39.5 36.5 40.5 31.5 41.0 54-55 36.83527395555289 40.0 36.5 41.0 31.0 41.0 56-57 36.766734391213696 40.0 36.0 41.0 31.0 41.0 58-59 36.534054131734614 39.0 36.0 41.0 31.0 41.0 60-61 36.23045120552677 39.0 35.0 41.0 31.0 41.0 62-63 35.88210524523199 38.5 35.0 41.0 30.5 41.0 64-65 35.484330589162724 37.5 35.0 40.0 30.0 41.0 66-67 35.08978426007269 37.0 35.0 40.0 29.0 41.0 68-69 34.67520950544022 36.5 35.0 39.0 29.0 41.0 70-71 34.24077692543821 36.0 34.0 39.0 29.0 41.0 72-73 33.79895605900828 35.5 34.0 38.5 28.0 40.0 74-75 33.34399228906334 35.0 34.0 37.0 27.5 39.0 76-77 32.87122914752827 35.0 34.0 37.0 26.0 39.0 78-79 32.42132807998911 35.0 33.0 36.0 25.5 38.5 80-81 31.965770706590554 35.0 33.0 36.0 24.5 37.0 82-83 31.53701137516946 35.0 33.0 35.0 23.5 37.0 84-85 31.122866487792074 35.0 32.0 35.0 20.0 36.5 86-87 30.751394711803 35.0 32.0 35.0 18.0 36.0 88-89 30.349620834925155 35.0 31.5 35.0 9.5 36.0 90-91 29.974423702015933 34.0 31.0 35.0 3.5 35.0 92-93 29.545162431608965 34.0 31.0 35.0 2.0 35.0 94-95 29.084283262493933 34.0 30.5 35.0 2.0 35.0 96-97 28.60532686115579 34.0 30.0 35.0 2.0 35.0 98-99 28.043361507196703 34.0 29.0 35.0 2.0 35.0 100-101 26.004811732912852 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1328595110707269 1101 2 0.1736259765523016 1101 3 0.152404869695971 1101 4 0.10503541366128388 1101 5 0.1272968577826319 1101 6 0.038501139464557355 1101 7 0.0794898446156509 1101 8 0.11152047288825884 1101 9 0.08645483505482332 1101 10-11 0.13056122264679715 1101 12-13 0.14528914203533105 1101 14-15 0.1704267449246828 1101 16-17 0.1699152534684174 1101 18-19 0.18820900560073284 1101 20-21 0.1379222253416117 1101 22-23 0.2185526213587181 1101 24-25 0.14434097242563126 1101 26-27 0.18702885543746817 1101 28-29 0.1986658380619204 1101 30-31 0.2106051740761714 1101 32-33 0.1638349895332709 1101 34-35 0.07128947173888633 1101 36-37 0.12728778434934895 1101 38-39 0.24541768900411398 1101 40-41 0.22417539412506216 1101 42-43 0.20984430615465754 1101 44-45 0.15615202062090816 1101 46-47 0.22692170226270036 1101 48-49 0.2462071527791494 1101 50-51 0.14420364383335027 1101 52-53 0.2056464086404901 1101 54-55 0.32470721871415975 1101 56-57 0.214298480565148 1101 58-59 0.18847093463156028 1101 60-61 0.22137972039854503 1101 62-63 0.2585916119836966 1101 64-65 0.2574615054823326 1101 66-67 0.2177653485355293 1101 68-69 0.23035687518866865 1101 70-71 0.23486221896298787 1101 72-73 0.16601564912041766 1101 74-75 0.20984849951639717 1101 76-77 0.16409744067009768 1101 78-79 0.20497439571618514 1101 80-81 0.19717352471774774 1101 82-83 0.09539499294911735 1101 84-85 0.15437600978443555 1101 86-87 0.16923646040652685 1101 88-89 0.1770340200830347 1101 90-91 0.10630451773372585 1101 92-93 0.1226279876897749 1101 94-95 0.1587246418762227 1101 96-97 0.16899500705184778 1101 98-99 0.09301193886503256 1101 100-101 0.4709425372224878 1102 1 0.18595324078351894 1102 2 0.21898139003980788 1102 3 0.1874658442659154 1102 4 0.17241705261957918 1102 5 0.20640237692098395 1102 6 0.17614343015231526 1102 7 0.15930598392309747 1102 8 0.1984138005976277 1102 9 0.17697299456772697 1102 10-11 0.24467706130742073 1102 12-13 0.2171419667902299 1102 14-15 0.24386804143717455 1102 16-17 0.25151762075435613 1102 18-19 0.2492467645166272 1102 20-21 0.28550042030235545 1102 22-23 0.2913769998511171 1102 24-25 0.3023291129899093 1102 26-27 0.23777155145242546 1102 28-29 0.31117906263373385 1102 30-31 0.2991600028517283 1102 32-33 0.2809541338020125 1102 34-35 0.263133434689685 1102 36-37 0.3082752040074155 1102 38-39 0.3067951041608197 1102 40-41 0.27721777116901336 1102 42-43 0.272641248602028 1102 44-45 0.2896261566419014 1102 46-47 0.26740072832898676 1102 48-49 0.2834203157618731 1102 50-51 0.2532829290054437 1102 52-53 0.25539857174667446 1102 54-55 0.3614400462731808 1102 56-57 0.29738088215049174 1102 58-59 0.3087535583270764 1102 60-61 0.2753040107364413 1102 62-63 0.2549028703304188 1102 64-65 0.2459237816335289 1102 66-67 0.24433457990149776 1102 68-69 0.29554725507345836 1102 70-71 0.22782005501903768 1102 72-73 0.20139472731296593 1102 74-75 0.23041144134700886 1102 76-77 0.21184966343064815 1102 78-79 0.2521477053072445 1102 80-81 0.2493352131804727 1102 82-83 0.29112258965165694 1102 84-85 0.2662937294301493 1102 86-87 0.29605492623403507 1102 88-89 0.28821813681805963 1102 90-91 0.24446835775312792 1102 92-93 0.33774282140386447 1102 94-95 0.2935592593079086 1102 96-97 0.4045780301401294 1102 98-99 0.4275087518525176 1102 100-101 0.7062154754558563 1103 1 0.0025097336666064507 1103 2 0.05351185526174618 1103 3 -0.013358635289506537 1103 4 -0.008320750203054672 1103 5 -0.012043252019424244 1103 6 -0.011877034155588717 1103 7 -0.024053372499189152 1103 8 0.018330145763933103 1103 9 -6.501856014224927E-4 1103 10-11 -0.02108690075855435 1103 12-13 -0.005926503167451358 1103 14-15 -0.022664816116481745 1103 16-17 -0.012003647916444038 1103 18-19 -0.07789896894908566 1103 20-21 -0.05303244526276529 1103 22-23 -0.14196782062355595 1103 24-25 -0.11565269783592669 1103 26-27 -0.07037534191514538 1103 28-29 -0.04207933237672279 1103 30-31 -0.03568017819760172 1103 32-33 -0.08752286729422565 1103 34-35 -0.12071071075864381 1103 36-37 -0.10002964868061781 1103 38-39 -0.1495014161821615 1103 40-41 -0.07614090367209059 1103 42-43 -0.06758486519447615 1103 44-45 -0.12717818173456408 1103 46-47 -0.18879570943524726 1103 48-49 -0.15023843110956392 1103 50-51 -0.14920929714549658 1103 52-53 -0.09796538337602811 1103 54-55 -0.05630611681322506 1103 56-57 -0.0754790106148775 1103 58-59 0.020084129203205237 1103 60-61 0.0655232556149059 1103 62-63 0.061100998391701467 1103 64-65 0.03941779494736153 1103 66-67 -0.0183322618237014 1103 68-69 -0.020608133660267924 1103 70-71 -0.0564338069000172 1103 72-73 -0.06381737704676738 1103 74-75 -0.0017168690102238315 1103 76-77 -0.04717377640697862 1103 78-79 -0.08385124719413284 1103 80-81 -0.08162671483605521 1103 82-83 -0.09159741722606185 1103 84-85 -0.10734509590990982 1103 86-87 -0.20835230328475163 1103 88-89 -0.1592660639117831 1103 90-91 -0.20012528330692447 1103 92-93 -0.1156581472789675 1103 94-95 -0.06406247667388243 1103 96-97 -0.10994617339615331 1103 98-99 -0.1743767851163156 1103 100-101 0.042908656014660096 1104 1 0.0952907019257907 1104 2 0.13834315922451168 1104 3 0.1423756835707053 1104 4 0.1350763618964308 1104 5 0.15130074534093296 1104 6 0.12698826027665433 1104 7 0.15549656874951978 1104 8 0.11149561414657683 1104 9 0.18193987076987383 1104 10-11 0.19594223652116227 1104 12-13 0.15250229688269457 1104 14-15 0.19362217433663176 1104 16-17 0.12805762605493953 1104 18-19 0.25282511085364234 1104 20-21 0.22795461055018507 1104 22-23 0.21765742240257424 1104 24-25 0.2185921374883435 1104 26-27 0.2747293123352179 1104 28-29 0.23259116327414375 1104 30-31 0.28021951016857116 1104 32-33 0.24860535854244148 1104 34-35 0.29186889785614767 1104 36-37 0.22409217130045533 1104 38-39 0.14917148671815994 1104 40-41 0.13331669717824468 1104 42-43 0.23772433116977965 1104 44-45 0.21941004967126787 1104 46-47 0.1758875349969813 1104 48-49 0.23671571995663498 1104 50-51 0.15103029664724232 1104 52-53 0.1777367470522293 1104 54-55 0.3623732199318326 1104 56-57 0.2464348758703494 1104 58-59 0.1581921617556148 1104 60-61 0.21493886481984958 1104 62-63 0.2357186530970452 1104 64-65 0.3428735641246021 1104 66-67 0.28902492135114244 1104 68-69 0.29116294994864944 1104 70-71 0.24427208209240803 1104 72-73 0.23123045131111297 1104 74-75 0.23178237115155298 1104 76-77 0.21986835082417855 1104 78-79 0.18484860425301974 1104 80-81 0.19753650362175534 1104 82-83 0.20356004290716712 1104 84-85 0.17043865861077023 1104 86-87 0.18409487764577293 1104 88-89 0.23228051748088063 1104 90-91 0.2763673788873575 1104 92-93 0.23144135861504722 1104 94-95 0.22922274439570245 1104 96-97 0.3078164654790214 1104 98-99 0.3207476764851833 1104 100-101 0.5267287270918466 1105 1 -0.11504623890429855 1105 2 0.03776264310882027 1105 3 -0.029759127129693752 1105 4 0.06923659576352748 1105 5 0.11023193469512194 1105 6 0.1140023280975484 1105 7 0.11909742114916355 1105 8 0.08698652051199218 1105 9 0.10830187734149632 1105 10-11 0.07368367709511858 1105 12-13 0.12778909366979718 1105 14-15 0.1515518927218622 1105 16-17 0.15515463862855228 1105 18-19 0.1864274751112589 1105 20-21 0.10110895019887778 1105 22-23 0.19744367786604045 1105 24-25 0.19687240648877946 1105 26-27 0.24158876277426344 1105 28-29 0.23312292838888737 1105 30-31 0.2759379645788371 1105 32-33 0.29411191505938206 1105 34-35 0.22264080623387628 1105 36-37 0.21990609420237917 1105 38-39 0.23816917812970928 1105 40-41 0.1767264989572439 1105 42-43 0.125434687337048 1105 44-45 0.15289333906751068 1105 46-47 0.15114572187053454 1105 48-49 0.18788012192162284 1105 50-51 0.08875786763108096 1105 52-53 0.19436392437970795 1105 54-55 0.4603213322486397 1105 56-57 0.37092249948797473 1105 58-59 0.33475597671301927 1105 60-61 0.3552457648395446 1105 62-63 0.40299661642655593 1105 64-65 0.41501098308634 1105 66-67 0.45330742490799025 1105 68-69 0.40850126433175404 1105 70-71 0.43545186492197985 1105 72-73 0.4289279845479541 1105 74-75 0.34874173638009864 1105 76-77 0.341004741550357 1105 78-79 0.41461510811779334 1105 80-81 0.5187293103987614 1105 82-83 0.5876391281916682 1105 84-85 0.6092049536249959 1105 86-87 0.5759885647970506 1105 88-89 0.6470342950580132 1105 90-91 0.5212008843615621 1105 92-93 0.46446186542794266 1105 94-95 0.48227954499002124 1105 96-97 0.42067958399708516 1105 98-99 0.38841070189941007 1105 100-101 0.6407380145444606 1106 1 -0.13670939812093508 1106 2 -0.017660224249517853 1106 3 -0.04536633031823811 1106 4 0.07958919328312675 1106 5 0.09425281213174941 1106 6 0.10269625804220084 1106 7 0.09770899591379845 1106 8 0.08139273867779906 1106 9 -0.023209736245092927 1106 10-11 -0.018102434160887526 1106 12-13 0.09805887804790103 1106 14-15 0.09348068405133603 1106 16-17 -0.022021303746662113 1106 18-19 0.16421046715098697 1106 20-21 0.15067267124907602 1106 22-23 0.21872098861202716 1106 24-25 0.19801160268618645 1106 26-27 0.19075004131263285 1106 28-29 0.1845732920040959 1106 30-31 0.15456000958911176 1106 32-33 0.2228091882731107 1106 34-35 0.034736602737446276 1106 36-37 0.14452849715643623 1106 38-39 0.1236328481189517 1106 40-41 0.18462742541483834 1106 42-43 0.26499019461938644 1106 44-45 0.19776017616050723 1106 46-47 0.14445202782290067 1106 48-49 0.1651726131004665 1106 50-51 0.08027410059935391 1106 52-53 0.16111710378432775 1106 54-55 0.3284247427714817 1106 56-57 0.24130451697449473 1106 58-59 0.3005280399591328 1106 60-61 0.38001322421731487 1106 62-63 0.35538527540609266 1106 64-65 0.19850537145595126 1106 66-67 0.34701270307833454 1106 68-69 0.31201364515939645 1106 70-71 0.32050712094773104 1106 72-73 0.3254883125276251 1106 74-75 0.3545496150328802 1106 76-77 0.28382260256922365 1106 78-79 0.16051158397358023 1106 80-81 0.3273398504407865 1106 82-83 0.45722319265948386 1106 84-85 0.33524558743311417 1106 86-87 0.36164748459055573 1106 88-89 0.41253370063429173 1106 90-91 0.22393335955816696 1106 92-93 0.3295441176506593 1106 94-95 0.32203120183540435 1106 96-97 0.31862526158193916 1106 98-99 0.33699725414307835 1106 100-101 0.6585811397961265 1107 1 0.047659324495207045 1107 2 0.07581392618456562 1107 3 0.0817780543270814 1107 4 0.1139489594472991 1107 5 0.1127906744371927 1107 6 0.08453807361879484 1107 7 0.10958340736229388 1107 8 0.06997080311944615 1107 9 0.069854498638108 1107 10-11 0.0626699474687129 1107 12-13 0.1263294926309939 1107 14-15 0.16634400927849669 1107 16-17 0.16044016272191186 1107 18-19 0.164973488298628 1107 20-21 0.17821234900851834 1107 22-23 0.18872495581372561 1107 24-25 0.20690074774078226 1107 26-27 0.21283631954019455 1107 28-29 0.21353683163803794 1107 30-31 0.24677601537513993 1107 32-33 0.23376390960863347 1107 34-35 0.24262217136212172 1107 36-37 0.19665531641108913 1107 38-39 0.2418128565850779 1107 40-41 0.1901995205385063 1107 42-43 0.14641317275244603 1107 44-45 0.18058051300143774 1107 46-47 0.18971925281986302 1107 48-49 0.18688154881893126 1107 50-51 0.1267075459158491 1107 52-53 0.21432597218889526 1107 54-55 0.3618987986735789 1107 56-57 0.3402609745987064 1107 58-59 0.33090598477802047 1107 60-61 0.3162728326799211 1107 62-63 0.3129407518064298 1107 64-65 0.3359604730383481 1107 66-67 0.23217600963437235 1107 68-69 0.24209805978029664 1107 70-71 0.26207589784505814 1107 72-73 0.3593612389581935 1107 74-75 0.3449641972104658 1107 76-77 0.3631110938143465 1107 78-79 0.3611800228659874 1107 80-81 0.42453464095193993 1107 82-83 0.5039703773534399 1107 84-85 0.43443493654624277 1107 86-87 0.3977863616026305 1107 88-89 0.46058141228263594 1107 90-91 0.4618628762712049 1107 92-93 0.5491305191223823 1107 94-95 0.5642578885490899 1107 96-97 0.6564110980525939 1107 98-99 0.6667204790765453 1107 100-101 0.8191915514081565 1108 1 0.19519232579952472 1108 2 0.18932918300169632 1108 3 0.18896655649427174 1108 4 0.09175070212000236 1108 5 0.1524749807932153 1108 6 0.1629467216625713 1108 7 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19627.0 4 15496.0 5 13082.0 6 12003.0 7 12077.0 8 13086.0 9 15331.0 10 17267.0 11 20596.0 12 24758.0 13 28190.0 14 29633.0 15 32828.0 16 38924.0 17 48101.0 18 45433.0 19 37225.0 20 38535.0 21 42236.0 22 43738.0 23 48717.0 24 56218.0 25 66432.0 26 77229.0 27 93374.0 28 115728.0 29 141964.0 30 175710.0 31 219356.0 32 276664.0 33 361553.0 34 506235.0 35 775356.0 36 1416518.0 37 2888479.0 38 3170489.0 39 491577.0 40 47.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.936145982828556 18.311876704321577 13.940818185594612 24.81115912725526 2 16.01753360668789 20.567082655103913 38.409117141722774 25.00626659648542 3 18.16325021276884 25.554882802672097 29.8043586912889 26.47750829327016 4 13.571547445908852 17.609860659865724 36.76544215192233 32.05314974230309 5 15.01631366178775 35.82575048233434 33.33765629145128 15.82027956442662 6 31.325215241484393 35.15763431251997 19.252477268297056 14.264673177698576 7 28.62465630943184 31.19858220932384 22.41636424677741 17.760397234466907 8 27.29640840459956 32.950022025688874 21.128589691838535 18.62497987787303 9 27.40566119724675 16.679908572620455 20.109608565667802 35.804821664464995 10-11 25.432780395790715 25.2733356729452 28.038440553087028 21.255443378177052 12-13 26.553006215596795 22.743762002583438 27.74090402217831 22.962327759641457 14-15 23.75792645770031 24.038366849634563 25.043377845873948 27.160328846791177 16-17 24.038970120705415 26.577060799490283 25.770790532884867 23.613178546919432 18-19 24.196362015769804 25.755527296312337 26.811741502592902 23.23636918532496 20-21 24.493476293300702 25.46304710483815 26.473756412298993 23.569720189562158 22-23 24.35331218492912 25.41888734834798 26.53570006892666 23.692100397796242 24-25 24.335063376573334 25.727308819518473 26.222432256243206 23.715195547664987 26-27 24.128774865686424 25.976279082662106 25.908760541570135 23.986185510081327 28-29 24.08899513800776 25.83366673788857 26.00194858612786 24.07538953797581 30-31 24.126749734634053 25.505913922400904 26.17991539490107 24.187420948063973 32-33 23.89620534094288 25.806668064875378 26.184658115806275 24.11246847837547 34-35 24.519886811821394 25.58921609233068 25.89106137603343 23.999835719814495 36-37 24.28662487143595 25.379191161989574 25.968860600500363 24.365323366074108 38-39 24.247348517073515 25.42807081605166 26.204656446247732 24.119924220627084 40-41 24.356933853732023 24.920396397739843 26.63970472388428 24.082965024643855 42-43 23.853490374805776 25.260564888540443 26.530215150837964 24.355729585815812 44-45 23.78968675646162 25.47683029202052 26.229208517660208 24.504274433857653 46-47 24.016861023644797 25.319369234519257 26.215650432909364 24.448119308926575 48-49 23.900330388435847 25.490524623741663 26.10325890091615 24.505886086906344 50-51 23.447903204165158 25.705653781365385 26.21728677309444 24.629156241375018 52-53 23.882489568135476 25.335882680769195 26.04303979263376 24.73858795846157 54-55 23.519856209028774 25.244316291121795 26.355399109545107 24.880428390304328 56-57 23.50410699152556 25.63483765072255 26.320342166951043 24.54071319080085 58-59 23.868104560883726 25.328559297884638 26.040315023420447 24.763021117811192 60-61 23.75658537759612 25.079348678205665 26.484113361784694 24.67995258241352 62-63 23.908874401154133 25.100506686403367 26.319918145944992 24.670700766497507 64-65 23.741467622208106 25.17282012311765 25.973437236508325 25.11227501816592 66-67 23.6023134218564 25.786695260731836 25.690525730530826 24.920465586880937 68-69 23.599031247045215 25.16454686778684 26.15114152108258 25.085280364085367 70-71 23.661119826208164 25.26279179211836 25.874025342411905 25.202063039261567 72-73 23.766017450959236 25.512725510557168 25.581856161229588 25.13940087725401 74-75 23.908130645194937 25.49267906436652 25.679870077946276 24.919320212492266 76-77 24.052645569010693 25.39092848426942 25.855733012204198 24.700692934515697 78-79 23.866502437473805 25.576842727806625 25.833042006852345 24.72361282786722 80-81 24.127281232936674 25.59799356396334 25.794725557673825 24.479999645426158 82-83 24.119380702503705 25.547168794761355 25.832807229685194 24.50064327304975 84-85 24.13163423862756 25.5643219315014 25.734535022925403 24.569508806945638 86-87 24.42945457253663 25.49538750109389 25.688950555148416 24.386207371221065 88-89 24.264161882843087 25.34073349388028 25.716298102104606 24.678806521172028 90-91 24.471895362638698 25.43575978911229 25.86281478951532 24.229530058733694 92-93 24.81870144075746 25.206875498500413 25.726224268338733 24.248198792403393 94-95 24.79093041865566 25.115926590593514 25.586563027988895 24.506579962761933 96-97 25.18439388663211 25.006448828739636 25.35799913934445 24.451158145283806 98-99 24.932640939066715 24.795168676653073 25.52102274389697 24.75116764038324 100-101 24.983686922477055 24.650499270607174 25.71837106442657 24.6474427424892 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 949.0 1 738.0 2 1239.0 3 1624.0 4 946.0 5 654.5 6 902.0 7 1539.5 8 2477.5 9 3035.0 10 2841.0 11 2647.0 12 2827.0 13 3127.0 14 3388.5 15 3568.5 16 3874.5 17 4215.5 18 4481.0 19 4927.5 20 5576.5 21 6423.0 22 7656.5 23 9293.5 24 11570.5 25 14565.5 26 18309.5 27 23561.0 28 29610.0 29 37530.5 30 50136.5 31 67526.5 32 87570.0 33 107053.5 34 133790.0 35 161137.0 36 184652.0 37 210259.5 38 232085.5 39 240553.0 40 250531.0 41 284197.0 42 322690.5 43 358402.0 44 389454.5 45 410961.0 46 427442.0 47 451677.5 48 489603.0 49 515245.5 50 530600.5 51 541462.0 52 533037.5 53 529397.0 54 530352.0 55 503754.5 56 470986.0 57 433335.5 58 388258.0 59 338051.5 60 273904.0 61 211308.5 62 162683.5 63 125150.0 64 93644.0 65 70421.5 66 51955.5 67 39805.5 68 28195.0 69 18299.0 70 13084.5 71 9090.0 72 6449.0 73 4719.5 74 3493.0 75 2635.0 76 1851.0 77 1218.5 78 799.5 79 542.5 80 397.5 81 306.0 82 260.5 83 217.5 84 175.0 85 139.5 86 122.5 87 106.0 88 84.5 89 81.0 90 68.5 91 55.0 92 55.0 93 45.0 94 41.5 95 43.5 96 34.5 97 25.5 98 16.5 99 10.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022494721038958963 2 0.034050525975928995 3 0.023876204486311017 4 0.02286832977000386 5 0.04256532961369639 6 0.02293783837112849 7 0.026187365473705024 8 0.04013252857433428 9 0.025779002442097812 10-11 0.027673111822744033 12-13 0.02597883967033113 14-15 0.03536250082215642 16-17 0.04267828109052392 18-19 0.04488952346380127 20-21 0.0376041532084258 22-23 0.044402963255928844 24-25 0.019431998301904875 26-27 0.025179490757397865 28-29 0.026721712844850632 30-31 0.026817287171397 32-33 0.03623135833621432 34-35 0.040245480051161804 36-37 0.0297931241570453 38-39 0.015205006496013203 40-41 0.01768993898621879 42-43 0.019988067110901928 44-45 0.021908242216969884 46-47 0.015309269397700151 48-49 0.02529244223422539 50-51 0.01820256491951295 52-53 0.027408110280956372 54-55 0.023146364174502384 56-57 0.02247299960110752 58-59 0.022068980857070594 60-61 0.019497162615459217 62-63 0.022116768020343777 64-65 0.019492818327888926 66-67 0.018106990592966582 68-69 0.022720623992614018 70-71 0.035697010965068714 72-73 0.025140392169265263 74-75 0.03481512058829995 76-77 0.043104021272412286 78-79 0.026843352896818738 80-81 0.022555541064943015 82-83 0.0157436981547291 84-85 0.032921011207653735 86-87 0.019670934118270797 88-89 0.03032747152819091 90-91 0.02406300885183347 92-93 0.04225254090863555 94-95 0.07619880398287761 96-97 0.09692105569315845 98-99 0.07386592155763215 100-101 0.08159440914517714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1509367E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.48878285414418 #Duplication Level Percentage of deduplicated Percentage of total 1 75.59481514175643 29.8514724003199 2 12.456617776735104 9.837933489651295 3 4.065905069808485 4.816729272216936 4 2.014478873864496 3.181972752571839 5 1.1908480290801207 2.3512569616315226 6 0.8157699620431255 1.9328257734032652 7 0.5785542778153692 1.5992482972191124 8 0.43998501143793667 1.389957806060066 9 0.3320155735307719 1.1799801798615712 >10 2.1204332763425655 15.903823397937655 >50 0.20466519289993232 5.6545008297241806 >100 0.16292296035792853 12.853692622559032 >500 0.015774622180075474 4.2559436119207 >1k 0.0070593256694968555 4.711189316064024 >5k 1.1064748436118557E-4 0.2976914970159348 >10k+ 4.425899374447423E-5 0.18178179184305393 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.688575140578973E-6 0.0 0.0 1.7377150281157947E-5 3 0.0 8.688575140578973E-6 0.0 0.0 1.7377150281157947E-5 4 0.0 8.688575140578973E-6 0.0 1.7377150281157947E-5 1.7377150281157947E-5 5 8.688575140578973E-6 1.7377150281157947E-5 0.0 1.7377150281157947E-5 1.7377150281157947E-5 6 8.688575140578973E-6 1.7377150281157947E-5 0.0 3.4754300562315894E-5 1.7377150281157947E-5 7 8.688575140578973E-6 1.7377150281157947E-5 0.0 4.344287570289487E-5 1.7377150281157947E-5 8 1.7377150281157947E-5 1.7377150281157947E-5 0.0 6.950860112463179E-5 1.7377150281157947E-5 9 1.7377150281157947E-5 1.7377150281157947E-5 0.0 1.2164005196810563E-4 1.7377150281157947E-5 10-11 1.7377150281157947E-5 1.7377150281157947E-5 0.0 2.6065725421736924E-4 1.7377150281157947E-5 12-13 3.4754300562315894E-5 1.7377150281157947E-5 0.0 4.257401818883697E-4 4.7787163273184356E-5 14-15 4.344287570289487E-5 1.7377150281157947E-5 0.0 6.342659852622651E-4 7.385288869492128E-5 16-17 5.213145084347384E-5 1.7377150281157947E-5 0.0 8.167260632144235E-4 9.557432654636871E-5 18-19 5.213145084347384E-5 1.7377150281157947E-5 0.0 0.0011990233693998983 9.557432654636871E-5 20-21 5.213145084347384E-5 1.7377150281157947E-5 0.0 0.0017029607275534787 9.991861411665819E-5 22-23 6.082002598405282E-5 1.7377150281157947E-5 0.0 0.0032104285144439306 1.0426290168694768E-4 24-25 7.819717626521076E-5 1.7377150281157947E-5 0.0 0.006503398492723362 1.0426290168694768E-4 26-27 7.819717626521076E-5 1.7377150281157947E-5 0.0 0.011186540493495429 1.0426290168694768E-4 28-29 9.557432654636871E-5 1.7377150281157947E-5 0.0 0.027434176006378107 1.0426290168694768E-4 30-31 1.0426290168694768E-4 2.1721437851447432E-5 0.0 0.08308449978178643 1.0426290168694768E-4 32-33 1.0860718925723717E-4 2.606572542173692E-5 0.0 0.20144027034675321 1.1729576439781614E-4 34-35 1.303286271086846E-4 3.4754300562315894E-5 0.0 0.3433594566929702 1.4770577738984256E-4 36-37 1.4770577738984256E-4 3.4754300562315894E-5 0.0 0.5545743740728748 1.4770577738984256E-4 38-39 1.5639435253042152E-4 3.4754300562315894E-5 0.0 0.9076042148973094 1.5639435253042152E-4 40-41 1.650829276710005E-4 3.4754300562315894E-5 0.0 1.329108716404647 1.5639435253042152E-4 42-43 1.7377150281157946E-4 3.4754300562315894E-5 0.0 1.7539539750535367 1.5639435253042152E-4 44-45 1.9114865309273742E-4 3.4754300562315894E-5 0.0 2.189151670982427 1.5639435253042152E-4 46-47 2.0418151580360588E-4 3.4754300562315894E-5 0.0 2.6569271794009177 1.60738640100711E-4 48-49 2.259029536550533E-4 3.4754300562315894E-5 0.0 3.144669033492459 1.8680436552244792E-4 50-51 2.259029536550533E-4 3.4754300562315894E-5 0.0 3.6671174009830425 1.9549294066302693E-4 52-53 2.302472412253428E-4 3.9098588132605386E-5 0.0 4.172888048491285 2.259029536550533E-4 54-55 2.519686790767902E-4 6.082002598405282E-5 0.0 4.670004875159512 2.3893581636592176E-4 56-57 2.7803440449852715E-4 6.082002598405282E-5 0.0 5.190498313243465 2.4328010393621127E-4 58-59 2.7803440449852715E-4 6.082002598405282E-5 0.0 5.7263705293262435 2.476243915065007E-4 60-61 2.910672672093956E-4 6.950860112463179E-5 0.0 6.290854223346948 2.519686790767902E-4 62-63 3.041001299202641E-4 6.950860112463179E-5 0.0 6.8594954005724205 2.693458293579482E-4 64-65 3.1278870506084303E-4 6.950860112463179E-5 0.0 7.406245712731204 2.693458293579482E-4 66-67 3.2147728020142204E-4 6.950860112463179E-5 0.0 7.9692349718277296 2.693458293579482E-4 68-69 3.30165855342001E-4 6.950860112463179E-5 0.0 8.560001605648687 2.693458293579482E-4 70-71 3.3885443048257995E-4 7.385288869492127E-5 0.0 9.173197796195048 2.7369011692823764E-4 72-73 3.475430056231589E-4 7.819717626521076E-5 0.0 9.770676354312101 2.9975584234997457E-4 74-75 3.562315807637379E-4 8.254146383550025E-5 0.0 10.359783470281208 3.127887050608431E-4 76-77 3.562315807637379E-4 9.123003897607923E-5 0.0 10.959660075137059 3.2147728020142204E-4 78-79 3.562315807637379E-4 9.557432654636871E-5 0.0 11.570623301872292 3.2147728020142204E-4 80-81 3.8664159375576435E-4 9.557432654636871E-5 0.0 12.205749456073473 3.258215677717115E-4 82-83 3.909858813260538E-4 9.557432654636871E-5 0.0 12.868913642253306 3.562315807637379E-4 84-85 3.953301688963433E-4 9.557432654636871E-5 0.0 13.519179638636947 3.649201559043169E-4 86-87 4.0836303160721177E-4 9.557432654636871E-5 0.0 14.150495852638986 3.692644434746064E-4 88-89 4.170516067477907E-4 9.557432654636871E-5 0.0 14.780682551872749 3.736087310448959E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9300 0.0 46.986668 1 TATAACG 890 0.0 46.43064 2 GTATCAA 16585 0.0 42.098988 1 ATAACGC 1140 0.0 38.331898 3 TATCACG 1090 0.0 37.039734 2 TAACGCA 1230 0.0 36.29961 4 TCAACGC 19500 0.0 34.929615 4 ATCAACG 19640 0.0 34.632103 3 CAACGCA 20535 0.0 33.30875 5 TATCAAC 21015 0.0 32.68243 2 AACGCAG 22020 0.0 31.471344 6 GTATAAC 2140 0.0 28.631607 1 TACCTGG 10600 0.0 28.095524 2 ATCACGC 1415 0.0 27.52547 3 ACGCAGA 25430 0.0 27.251595 7 GTACATG 30475 0.0 26.495674 1 CGCAGAG 26290 0.0 26.414227 8 ACATGGG 32530 0.0 23.785612 3 TACATGG 33630 0.0 23.643082 2 TATAAGC 5825 0.0 22.424053 3 >>END_MODULE