FastQCFastQC Report
Fri 27 May 2016
SRR940188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11509367
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128010.11122245037455145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93250.047.5037421
TATAACG10400.045.6717952
GTATCAA172100.042.6962131
ATAACGC11900.041.5103573
TAACGCA12950.037.4114274
TATCACG11250.036.73232
TCAACGC201500.035.4760554
ATCAACG201750.035.3140753
CAACGCA211250.033.9286355
TATCAAC220200.032.8305972
ATCACGC12800.032.283413
AACGCAG225500.032.12166
TACCTGG104700.030.7130812
GTATAAC23000.028.5175381
ACGCAGA265750.027.3100227
CGCAGAG274500.026.4740928
GTACATG305000.026.289121
ACATGGG321600.023.6748683
TACATGG336500.023.6293532
GTACCTG141400.022.9578951
Produced by FastQC (version 0.11.5)