FastQCFastQC Report
Fri 27 May 2016
SRR940187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723699
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129810.13349857909011786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85900.051.9390141
GTATCAA165250.045.3143731
ATCAACG202350.037.522383
TCAACGC202000.037.5154764
CAACGCA210150.036.036475
TATCAAC209800.034.8775062
AACGCAG222150.034.483436
ACGCAGA248950.030.619077
CGCAGAG261600.029.0658288
GTACATG270950.025.2343941
GCAGAGT312900.023.9363239
ACATGGG289050.023.1773053
TACATGG293450.022.571422
TACCTGG80900.020.850192
AGAGTAC280350.020.77124810-11
CAGAGTA294600.019.87938710-11
CATGGGG239750.019.6983094
GAGTACT155750.019.42677912-13
TATAACG5650.019.3423652
TATCACG9100.018.2749792
Produced by FastQC (version 0.11.5)