##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940187_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9723699 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67738542708901 33.0 31.0 34.0 28.0 34.0 2 30.655756518172765 33.0 31.0 34.0 26.0 34.0 3 31.495147782752223 34.0 31.0 34.0 28.0 34.0 4 35.237366150474216 37.0 35.0 37.0 33.0 37.0 5 35.40339010905212 37.0 35.0 37.0 35.0 37.0 6 35.553007348335235 37.0 36.0 37.0 35.0 37.0 7 35.5268412771724 37.0 36.0 37.0 35.0 37.0 8 35.51879989292141 37.0 36.0 37.0 35.0 37.0 9 37.25080517198239 39.0 38.0 39.0 35.0 39.0 10-11 37.18013062724381 39.0 38.0 39.0 35.0 39.0 12-13 37.09896902403088 39.0 38.0 39.0 34.5 39.0 14-15 38.49017683496784 41.0 39.0 41.0 34.5 41.0 16-17 38.40744072806038 41.0 39.0 41.0 34.0 41.0 18-19 38.34183025410391 41.0 39.0 41.0 34.0 41.0 20-21 38.26710642729685 40.0 39.0 41.0 34.0 41.0 22-23 38.15471895006211 40.0 38.0 41.0 34.0 41.0 24-25 38.041312004824505 40.0 38.0 41.0 34.0 41.0 26-27 37.8425151272165 40.0 38.0 41.0 33.0 41.0 28-29 37.66299908090532 40.0 38.0 41.0 33.0 41.0 30-31 37.629778595573555 40.0 38.0 41.0 33.0 41.0 32-33 37.63561886273938 40.0 38.0 41.0 33.0 41.0 34-35 37.631140011635495 40.0 38.0 41.0 33.0 41.0 36-37 37.71415996114236 40.0 38.0 41.0 33.0 41.0 38-39 37.62250122098597 40.0 38.0 41.0 33.0 41.0 40-41 37.48521005226509 40.0 38.0 41.0 32.5 41.0 42-43 37.203600656499134 40.0 38.0 41.0 31.0 41.0 44-45 37.133092252238576 40.0 38.0 41.0 31.0 41.0 46-47 36.9483247064723 40.0 37.0 41.0 31.0 41.0 48-49 36.7457240295077 40.0 37.0 41.0 30.5 41.0 50-51 35.81348322279412 39.0 36.0 40.0 29.0 40.5 52-53 35.88755945654015 39.0 36.0 40.0 29.5 41.0 54-55 36.2399817703119 40.0 36.0 41.0 30.0 41.0 56-57 36.06330960059542 39.5 35.5 41.0 29.5 41.0 58-59 35.796022326482955 39.0 35.0 41.0 29.0 41.0 60-61 35.524872376242826 39.0 35.0 41.0 28.0 41.0 62-63 35.17849683541212 38.5 35.0 40.5 28.0 41.0 64-65 34.78923915682705 37.5 35.0 40.0 27.5 41.0 66-67 34.40506904831176 37.0 34.5 40.0 26.5 41.0 68-69 34.00063201257052 36.5 34.0 39.0 26.0 41.0 70-71 33.55946960102323 36.0 34.0 39.0 26.0 41.0 72-73 33.08839979518083 35.0 34.0 38.5 25.5 40.0 74-75 32.6163504238459 35.0 33.0 37.0 23.5 39.5 76-77 32.13042212639449 35.0 33.0 37.0 22.0 39.0 78-79 31.64905603310016 35.0 32.5 36.0 20.0 38.5 80-81 31.194557184462415 35.0 32.0 36.0 17.5 37.0 82-83 30.771362729348162 35.0 32.0 35.0 12.0 37.0 84-85 30.392549687109813 35.0 31.0 35.0 7.0 36.5 86-87 30.002697224585006 34.5 31.0 35.0 2.0 36.0 88-89 29.58998067504969 34.0 31.0 35.0 2.0 36.0 90-91 29.20411028765905 34.0 30.0 35.0 2.0 35.0 92-93 28.790389233562248 34.0 30.0 35.0 2.0 35.0 94-95 28.336611303990384 34.0 29.0 35.0 2.0 35.0 96-97 27.8213749726313 34.0 29.0 35.0 2.0 35.0 98-99 27.19199103139659 34.0 27.0 35.0 2.0 35.0 100-101 25.105452513493063 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5363302963300676 1101 2 1.6211579137844474 1101 3 0.8926887338236646 1101 4 0.4935039532334926 1101 5 0.3854456271936826 1101 6 0.32859785934790153 1101 7 0.32150995246084335 1101 8 0.32772031548849867 1101 9 0.325801763725174 1101 10-11 0.3438292407604848 1101 12-13 0.3446997735124455 1101 14-15 0.44295157689151665 1101 16-17 0.44253023359368626 1101 18-19 0.4220546065392554 1101 20-21 0.4363129806119517 1101 22-23 0.4198642386310496 1101 24-25 0.4320944193205065 1101 26-27 0.5037787384907233 1101 28-29 0.4793761908624887 1101 30-31 0.47278827045745686 1101 32-33 0.5226042596654494 1101 34-35 0.5778002003537637 1101 36-37 0.6205872700553883 1101 38-39 0.6538586313782275 1101 40-41 0.6455659986862301 1101 42-43 0.7225587395633113 1101 44-45 0.6326443397834609 1101 46-47 0.6094072040435492 1101 48-49 0.6394907181445717 1101 50-51 0.44595604844815284 1101 52-53 0.5858840581822875 1101 54-55 0.608704090393914 1101 56-57 0.7168427927601257 1101 58-59 0.7214563851943936 1101 60-61 0.662603753086465 1101 62-63 0.6870779880934776 1101 64-65 0.6923022110405341 1101 66-67 0.7104903519040917 1101 68-69 0.6214222523477204 1101 70-71 0.5233802772303733 1101 72-73 0.6650539207358364 1101 74-75 0.6550419550746156 1101 76-77 0.6585174572281858 1101 78-79 0.6058411392395051 1101 80-81 0.6334382242674614 1101 82-83 0.7223829608629302 1101 84-85 0.6567813628229722 1101 86-87 0.575924305381001 1101 88-89 0.548793061710164 1101 90-91 0.5830312137632561 1101 92-93 0.6017925306712755 1101 94-95 0.6050602443474951 1101 96-97 0.528629754723422 1101 98-99 0.4918115110839736 1101 100-101 0.5517836994729564 1102 1 0.3445643442720616 1102 2 0.894283721914185 1102 3 0.5700273305601407 1102 4 0.33365543682090504 1102 5 0.2854033831433753 1102 6 0.2759556295804586 1102 7 0.23210552835165288 1102 8 0.2937859716374831 1102 9 0.3380198788799831 1102 10-11 0.3501311507930751 1102 12-13 0.38118847017500457 1102 14-15 0.4020833805107955 1102 16-17 0.3815145399829305 1102 18-19 0.4003983600800254 1102 20-21 0.3837556164600855 1102 22-23 0.4119274128636903 1102 24-25 0.4180900877062399 1102 26-27 0.46298045136016697 1102 28-29 0.41797753904494783 1102 30-31 0.43473002709706066 1102 32-33 0.42258295452672456 1102 34-35 0.48652028564755767 1102 36-37 0.510320102085636 1102 38-39 0.43744764936999303 1102 40-41 0.4853263717502827 1102 42-43 0.667345708117864 1102 44-45 0.46007990895274276 1102 46-47 0.51811135469395 1102 48-49 0.46728995588541977 1102 50-51 0.2755708196330673 1102 52-53 0.340253580719164 1102 54-55 0.5389063701879948 1102 56-57 0.5270002669206022 1102 58-59 0.5660609525636247 1102 60-61 0.5371840875065317 1102 62-63 0.5249753059788205 1102 64-65 0.5463306118051037 1102 66-67 0.5401678833846404 1102 68-69 0.4709055484893483 1102 70-71 0.46147283933515837 1102 72-73 0.47079879050182427 1102 74-75 0.45244481012625215 1102 76-77 0.46748968489433906 1102 78-79 0.5278214440453475 1102 80-81 0.5394597610824832 1102 82-83 0.47215679051699055 1102 84-85 0.42911280922529116 1102 86-87 0.47441633751999035 1102 88-89 0.5077273409697511 1102 90-91 0.4269753870505042 1102 92-93 0.49873835682189593 1102 94-95 0.4212254302482812 1102 96-97 0.3667020903036722 1102 98-99 0.47419199705685955 1102 100-101 0.4321253638132845 1103 1 0.375513798174989 1103 2 1.3563555077345946 1103 3 0.6794363881237935 1103 4 0.40066706483894166 1103 5 0.3267412314249185 1103 6 0.2444202829232296 1103 7 0.2101352375312615 1103 8 0.23265844959849602 1103 9 0.23454600846184093 1103 10-11 0.23596029363342552 1103 12-13 0.28436586849837653 1103 14-15 0.3025777163202221 1103 16-17 0.32435687531712176 1103 18-19 0.2410996862040662 1103 20-21 0.2296348554265748 1103 22-23 0.3234313052583957 1103 24-25 0.33141718858893654 1103 26-27 0.35485647612926385 1103 28-29 0.35473251176151877 1103 30-31 0.2682885771010035 1103 32-33 0.24497184820196338 1103 34-35 0.30942386412081646 1103 36-37 0.272024754144816 1103 38-39 0.2811474068197626 1103 40-41 0.4043890921306499 1103 42-43 0.49930378242491713 1103 44-45 0.3107985881874953 1103 46-47 0.18015118808463626 1103 48-49 0.17914463754122778 1103 50-51 0.17960109911468436 1103 52-53 0.25156799034425603 1103 54-55 0.3324397081191677 1103 56-57 0.4311674196215236 1103 58-59 0.341700328318133 1103 60-61 0.35017612736422166 1103 62-63 0.3286020172377917 1103 64-65 0.4021000165655124 1103 66-67 0.3648205283836461 1103 68-69 0.2786686679951913 1103 70-71 0.3378746650283375 1103 72-73 0.39282205179210195 1103 74-75 0.4078097038537294 1103 76-77 0.42191515042595285 1103 78-79 0.409282674520302 1103 80-81 0.38034053420262737 1103 82-83 0.5152167038761633 1103 84-85 0.43625261451090935 1103 86-87 0.3808760844191319 1103 88-89 0.3466231669890405 1103 90-91 0.28742205152399336 1103 92-93 0.2944880661773013 1103 94-95 0.3233390488555479 1103 96-97 0.1646783050662357 1103 98-99 0.2476315373955984 1103 100-101 0.35903132047637243 1104 1 0.49308192787759353 1104 2 1.5095235131077196 1104 3 0.8092900280248081 1104 4 0.5337822469380242 1104 5 0.4174744416642042 1104 6 0.30305937491379353 1104 7 0.3261522414232374 1104 8 0.3124708578292328 1104 9 0.26906939779181016 1104 10-11 0.32159561881964294 1104 12-13 0.3053816539479328 1104 14-15 0.3065746336537032 1104 16-17 0.28743830926654823 1104 18-19 0.24257781081694674 1104 20-21 0.24857566143928267 1104 22-23 0.25149519713254165 1104 24-25 0.29519995119847664 1104 26-27 0.30063686563721603 1104 28-29 0.2592809077726841 1104 30-31 0.1797302710275943 1104 32-33 0.2317933363068363 1104 34-35 0.16939998897015585 1104 36-37 0.26624698109172584 1104 38-39 0.3158791931406384 1104 40-41 0.31256100758103855 1104 42-43 0.390854972401911 1104 44-45 0.2004965437565147 1104 46-47 0.1584410173489914 1104 48-49 0.09554572015210283 1104 50-51 0.06368575579525526 1104 52-53 0.14975440020759834 1104 54-55 0.2490967498329013 1104 56-57 0.20468181412172015 1104 58-59 0.16356740972928918 1104 60-61 0.14823417579057008 1104 62-63 0.16954585113619203 1104 64-65 0.22775642039865573 1104 66-67 0.21100457453093924 1104 68-69 0.08462524729457499 1104 70-71 0.06911765491415167 1104 72-73 0.12916716458337163 1104 74-75 0.02262932289809072 1104 76-77 0.019936281650977605 1104 78-79 0.04187506844194644 1104 80-81 -0.03086145769335147 1104 82-83 -0.01730669445624855 1104 84-85 -0.1881578152221728 1104 86-87 -0.2209381880835899 1104 88-89 -0.1993560352594237 1104 90-91 -0.15462708850561668 1104 92-93 -0.20765471889812304 1104 94-95 -0.31446173750789796 1104 96-97 -0.419338483724804 1104 98-99 -0.40424799324572547 1104 100-101 -0.32242255404364073 1105 1 0.2865185077485286 1105 2 1.2599646226073489 1105 3 0.5932772094395027 1105 4 0.35655528665594716 1105 5 0.19349315896398878 1105 6 0.0823734174637849 1105 7 0.027604564260258257 1105 8 0.05312437699203798 1105 9 0.010093234684603658 1105 10-11 -0.02077733429776174 1105 12-13 -0.09606590223940259 1105 14-15 -0.03830029988072425 1105 16-17 -0.17976448304542458 1105 18-19 -0.13478567039766176 1105 20-21 -0.07941969159374196 1105 22-23 -0.1173157225829442 1105 24-25 -0.05862484008273583 1105 26-27 -0.02270268233026229 1105 28-29 -0.042537054351448944 1105 30-31 -0.04631185357460055 1105 32-33 -0.08411970296494786 1105 34-35 -0.030849831291064334 1105 36-37 0.05609646846971117 1105 38-39 0.020115613115834208 1105 40-41 -0.09861628804127776 1105 42-43 0.04052790618577262 1105 44-45 0.019484199747068942 1105 46-47 -0.06266506566434771 1105 48-49 -0.230488064263902 1105 50-51 -0.2792012515340119 1105 52-53 -0.20312719331252538 1105 54-55 0.054168707673383665 1105 56-57 0.007933955173790253 1105 58-59 -0.027321155053734003 1105 60-61 -0.1463368307278472 1105 62-63 -0.039231113134924556 1105 64-65 0.0729087063257694 1105 66-67 -0.03374795580332801 1105 68-69 -0.19339859073561882 1105 70-71 -0.17972425928230962 1105 72-73 -0.3336078894995822 1105 74-75 -0.42706050016902 1105 76-77 -0.39990169264894604 1105 78-79 -0.2840343443692497 1105 80-81 -0.39032013991654324 1105 82-83 -0.3550929920061918 1105 84-85 -0.3602682581357328 1105 86-87 -0.33461908086094283 1105 88-89 -0.4815547423193358 1105 90-91 -0.6232776853767419 1105 92-93 -0.6666791032766675 1105 94-95 -0.6736612309779595 1105 96-97 -0.9294118351168557 1105 98-99 -1.1338973586336927 1105 100-101 -1.1747214661310004 1106 1 0.25216693500332 1106 2 1.291556087432916 1106 3 0.5540144279985562 1106 4 0.32823199851705454 1106 5 0.1568990810519324 1106 6 0.18938630128081968 1106 7 0.19877774456673336 1106 8 0.17587408817003336 1106 9 0.06879307095521625 1106 10-11 0.14404228237431482 1106 12-13 0.0750932881172588 1106 14-15 0.11615988590910575 1106 16-17 0.05580188452110946 1106 18-19 0.025985601374976852 1106 20-21 -0.09634158957595673 1106 22-23 -0.09793284389738233 1106 24-25 -0.034617933871658124 1106 26-27 0.03134532424598291 1106 28-29 -0.010927905315277542 1106 30-31 -7.982375716863999E-5 1106 32-33 0.06987342543068564 1106 34-35 0.06242940316872847 1106 36-37 0.08136167037509523 1106 38-39 0.032968555777998176 1106 40-41 0.025842583683484577 1106 42-43 0.17638296944249277 1106 44-45 0.058936499305715984 1106 46-47 -0.03226833874386159 1106 48-49 -0.07066720772935042 1106 50-51 -0.29065168268535047 1106 52-53 -0.06015332124492545 1106 54-55 0.01574525974564267 1106 56-57 0.15657301425292758 1106 58-59 -0.011687290930446181 1106 60-61 0.04684618410711039 1106 62-63 -0.011302755439373868 1106 64-65 -0.12900000650959242 1106 66-67 -0.03437105445711808 1106 68-69 -0.02109960666876276 1106 70-71 -0.1851073241027592 1106 72-73 -0.18380268177758552 1106 74-75 -0.22258727211517737 1106 76-77 -0.3090875203524561 1106 78-79 -0.2723263876944735 1106 80-81 -0.30719096950393876 1106 82-83 -0.23688059478551082 1106 84-85 -0.19683148833541608 1106 86-87 -0.2515024087292659 1106 88-89 -0.5186163473426042 1106 90-91 -0.6428983360812985 1106 92-93 -0.7425055643222152 1106 94-95 -0.7498975835883179 1106 96-97 -0.8194653083358112 1106 98-99 -0.8260520296399037 1106 100-101 -0.8170598872570025 1107 1 0.4390231493006418 1107 2 1.468291019943404 1107 3 0.7817302093317089 1107 4 0.43410783770620753 1107 5 0.2988188380460599 1107 6 0.2910883169782892 1107 7 0.24972743034719969 1107 8 0.23686724631883038 1107 9 0.18211421862767452 1107 10-11 0.31491940887052294 1107 12-13 0.3471066541771748 1107 14-15 0.31855885254686456 1107 16-17 0.36903587386240844 1107 18-19 0.32570038929740264 1107 20-21 0.3109559353926912 1107 22-23 0.24306504444774646 1107 24-25 0.23864014874596506 1107 26-27 0.3546964866259472 1107 28-29 0.3355764155470169 1107 30-31 0.22116662784029728 1107 32-33 0.21625217871926594 1107 34-35 0.20165810382606253 1107 36-37 0.2890522968039022 1107 38-39 0.31659618222729335 1107 40-41 0.21630072753354312 1107 42-43 0.31696993614283997 1107 44-45 0.06321585116035777 1107 46-47 -0.02820515689181491 1107 48-49 0.10426760969993154 1107 50-51 0.07669379499982654 1107 52-53 0.08334571316903805 1107 54-55 0.12528652526304285 1107 56-57 0.13264025101646126 1107 58-59 0.1746662814720139 1107 60-61 0.15078484672045533 1107 62-63 0.11575353706917468 1107 64-65 0.027655065833677384 1107 66-67 0.10036935241650724 1107 68-69 0.045698600865684114 1107 70-71 -0.07842952373559342 1107 72-73 0.008770447746535126 1107 74-75 0.03360643161494892 1107 76-77 0.02703536849870858 1107 78-79 0.032981879294158745 1107 80-81 0.027911567629931966 1107 82-83 0.10058684414051555 1107 84-85 -0.056646151768820374 1107 86-87 -0.026073168412029446 1107 88-89 -0.0050742683534927835 1107 90-91 -0.07347709314026574 1107 92-93 -0.1942793743517086 1107 94-95 -0.1433702132530108 1107 96-97 -0.006211658936621234 1107 98-99 0.05168754617897875 1107 100-101 -0.04541793994722809 1108 1 0.26576034428218165 1108 2 1.3409723707291832 1108 3 0.6229991496131682 1108 4 0.21106898288811493 1108 5 0.097950750735464 1108 6 0.05792642937715442 1108 7 0.04135044826573164 1108 8 0.06224494340835918 1108 9 0.057905023089844576 1108 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-1.9258095306385599 2316 94-95 -1.8202914428038959 2316 96-97 -1.5615201058729191 2316 98-99 -1.5090545728394957 2316 100-101 -1.5564768186598599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115138.0 3 25682.0 4 19533.0 5 15526.0 6 14128.0 7 13989.0 8 15219.0 9 17161.0 10 19337.0 11 22461.0 12 26082.0 13 29542.0 14 31261.0 15 35223.0 16 42285.0 17 51895.0 18 47758.0 19 39174.0 20 40284.0 21 42763.0 22 44998.0 23 49792.0 24 56630.0 25 64319.0 26 75893.0 27 89852.0 28 108747.0 29 131553.0 30 159964.0 31 197430.0 32 249135.0 33 324899.0 34 453621.0 35 692999.0 36 1230429.0 37 2348015.0 38 2409865.0 39 371080.0 40 37.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.92721342001957 18.495951670510237 14.016029497551905 24.560805411918285 2 16.907877765248088 20.621807360192694 38.48145103475924 23.988863839799983 3 18.6305840469224 25.1472036326437 29.353864062555797 26.868348257878104 4 13.48538048645306 17.135965175409773 36.8421290937154 32.536525244421775 5 14.905868262388559 36.11210213157955 33.65657122376353 15.325458382268364 6 31.649315824817336 35.44619778509476 18.58140989899495 14.323076491092953 7 28.548493352731807 31.290394413322204 22.064888199073994 18.096224034871994 8 26.553775173169686 34.33153786528559 20.626601480150285 18.488085481394442 9 26.93918921003671 16.792312336279235 20.089343841630917 36.17915461205314 10-11 25.468480942431622 25.661222714843383 27.754434670418206 21.115861672306792 12-13 26.52027384073422 22.880261767360658 27.688808333446048 22.91065605845907 14-15 23.49829329594716 24.200240589469914 25.144505400405574 27.15696071417734 16-17 23.668891888497015 27.090876432753813 25.80315330575116 23.437078372998013 18-19 23.910758576276837 26.126450639922393 27.10556121929365 22.85722956450712 20-21 24.271743584734455 25.688853390761295 26.831934591545124 23.20746843295913 22-23 24.189838976611963 25.600953075856992 26.825429667685047 23.383778279845995 24-25 24.130616669520737 25.772092949108576 26.68849644989615 23.408793931474538 26-27 23.844381688083928 26.07665288195792 26.47627298392849 23.60269244602966 28-29 23.78475031246493 26.025271215239915 26.491091085057423 23.69888738723773 30-31 23.849507628891427 25.69994869928912 26.589906745850623 23.86063692596883 32-33 23.64361051582824 25.91275259463215 26.66262900643317 23.781007883106444 34-35 24.146749016260035 25.84509879780717 26.403500389196154 23.604651796736636 36-37 23.93986842746816 25.69314020228603 26.412778422055403 23.95421294819041 38-39 23.88919950162084 25.697413007350413 26.646679457328702 23.76670803370004 40-41 24.02098948072912 25.279790574203805 26.959066285307166 23.740153659759912 42-43 23.73004816160494 25.561839963589517 26.767721182106406 23.940390692699133 44-45 23.560101468866822 25.740588642249314 26.650385070348108 24.048924818535756 46-47 23.74395191535816 25.642901735252128 26.58805405922195 24.025092290167766 48-49 23.696418862444762 25.792380915548286 26.402123466065046 24.109076755941906 50-51 23.32214899712288 25.98936684060485 26.519132240058557 24.169351922213714 52-53 23.617026298944392 25.75892384885966 26.427095581018484 24.19695427117746 54-55 23.347942884724386 25.622586680487014 26.661745619577275 24.36772481521132 56-57 23.375398740938266 25.903607084531334 26.56609765535083 24.154896519179577 58-59 23.601107221237257 25.638970279106665 26.373115043269312 24.386807456386773 60-61 23.477487220906106 25.404188102296523 26.8142636261693 24.30406105062807 62-63 23.577509573515172 25.467633351341323 26.707355734830806 24.247501340312695 64-65 23.472892682828487 25.533671899653697 26.409278708502587 24.58415670901523 66-67 23.355874045279567 25.986828384870208 26.15956742158328 24.497730148266943 68-69 23.395178914656594 25.602270488738338 26.520248671596413 24.482301925008652 70-71 23.44890498278951 25.66380611229353 26.292545536806404 24.594743368110553 72-73 23.521274042115063 25.836619648186137 26.113939994435896 24.5281663152629 74-75 23.526528280760566 25.928501272498806 26.177203502737314 24.367766944003314 76-77 23.792765412885924 25.813588162286262 26.158558998861757 24.23508742596606 78-79 23.716947056494508 25.884853589251968 26.109697683714884 24.28850167053864 80-81 23.846976699429288 25.955675597334054 26.143624321441276 24.05372338179538 82-83 23.834381317289164 25.927662903805714 26.173011648382428 24.06494413052269 84-85 23.86762983209684 25.907966790501437 26.073557566299055 24.150845811102666 86-87 24.040287041570828 25.905100583876127 26.050328970378267 24.004283404174775 88-89 24.005897416814786 25.73564828099074 26.025062169905205 24.233392132289268 90-91 24.222308163089064 25.75658066127845 26.12541545348343 23.895695722149053 92-93 24.37573845256458 25.682746115956874 26.055353730289628 23.88616170118892 94-95 24.362209472595566 25.553057468574202 25.974486783613777 24.110246275216454 96-97 24.69715085095662 25.393439087646446 25.79385474831582 24.115555313081114 98-99 24.538614469657176 25.26384115414504 25.901320664963812 24.296223711233974 100-101 24.637350158553406 25.141281302133127 26.073298149104684 24.148070390208783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 564.0 1 463.5 2 885.0 3 1282.5 4 914.0 5 727.0 6 1003.0 7 1840.0 8 3291.5 9 4126.0 10 3889.5 11 3696.5 12 3857.0 13 4187.5 14 4471.0 15 4769.5 16 5103.0 17 5408.0 18 5843.5 19 6392.0 20 7169.0 21 8251.5 22 9803.0 23 11959.5 24 14654.5 25 18489.0 26 23519.0 27 29912.0 28 37158.5 29 45613.0 30 57674.5 31 73098.0 32 89995.5 33 107212.5 34 128488.5 35 148661.5 36 167364.0 37 187652.0 38 203354.5 39 210983.0 40 221070.0 41 245974.0 42 273183.0 43 299362.0 44 324900.5 45 346046.5 46 362974.5 47 382969.0 48 411083.0 49 429311.5 50 438712.0 51 445028.0 52 438019.0 53 432488.0 54 427314.5 55 403341.5 56 374548.5 57 340704.5 58 301107.5 59 261629.5 60 216550.5 61 170734.0 62 133564.5 63 103773.5 64 77943.0 65 58269.0 66 43066.5 67 32494.5 68 23357.5 69 16175.0 70 11847.0 71 8532.0 72 6246.5 73 4571.5 74 3385.5 75 2567.5 76 1863.0 77 1253.5 78 835.0 79 602.0 80 454.5 81 357.0 82 289.5 83 234.5 84 194.0 85 167.0 86 144.0 87 142.0 88 132.0 89 100.5 90 86.5 91 81.5 92 82.5 93 73.5 94 71.0 95 62.0 96 47.5 97 43.5 98 35.0 99 26.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05903103335469352 2 0.056028060926196915 3 0.06209571069610444 4 0.06137582004543744 5 0.060491382960332275 6 0.011477113802062363 7 0.01320485136366315 8 0.014840031555892464 9 0.0060573656177551356 10-11 0.011363988128386121 12-13 0.015205118957302153 14-15 0.01793556135376054 16-17 0.03260076232306244 18-19 0.030739330783480647 20-21 0.030837030228928308 22-23 0.019668440991437518 24-25 0.007651409201374909 26-27 0.011209725846100336 28-29 0.01859374709151322 30-31 0.01657291119356944 32-33 0.01950903663307554 34-35 0.024476282122677797 36-37 0.022799965321838943 38-39 0.010088753261490302 40-41 0.011461687573833784 42-43 0.01258780223452001 44-45 0.023586702961496443 46-47 0.01552392767402611 48-49 0.015230829337683119 50-51 0.01512798781615926 52-53 0.012325556354634176 54-55 0.008597551199394387 56-57 0.012556949778062855 58-59 0.011945042724995908 60-61 0.013127720222520258 62-63 0.02146302554202881 64-65 0.015703900336692857 66-67 0.01859888916758941 68-69 0.025134467860430484 70-71 0.019185085840275392 72-73 0.025201314849420987 74-75 0.04158396922817129 76-77 0.04896799047358418 78-79 0.03733661438923603 80-81 0.019524462861304118 82-83 0.016655184410788528 84-85 0.022038938062562404 86-87 0.025288730142716268 88-89 0.02215720581231484 90-91 0.026168025151745235 92-93 0.05035120893808004 94-95 0.07813384597774982 96-97 0.09300987206617564 98-99 0.09693841818838696 100-101 0.08111625010194165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9723699.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.7771149628711 #Duplication Level Percentage of deduplicated Percentage of total 1 75.77113613852251 35.44355146019025 2 12.602451112267534 11.790126089850027 3 4.144438216329705 5.815945887053132 4 2.036962308612363 3.8113288033998343 5 1.2262417550697515 2.8680025774585296 6 0.8168114135404023 2.292484883649879 7 0.561063908807151 1.8371465674652951 8 0.4249942620661797 1.5904004364184205 9 0.3123770881651445 1.3150889068381098 >10 1.8536405037061523 15.992159259459168 >50 0.1469220991099671 4.774919763038364 >100 0.09366230093607328 8.236684670439706 >500 0.006630722487535145 2.0938244495098473 >1k 0.002579231466193366 1.7947157896667585 >5k 6.67041850362591E-5 0.20860813236945092 >10k+ 2.22347283454197E-5 0.13501232319329123 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12981 0.13349857909011786 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.0568304304771262E-5 1.0284152152385631E-5 2 0.0 0.0 0.0 2.0568304304771262E-5 1.0284152152385631E-5 3 0.0 0.0 0.0 2.0568304304771262E-5 1.0284152152385631E-5 4 0.0 0.0 0.0 9.255736937147067E-5 1.0284152152385631E-5 5 0.0 0.0 0.0 2.1596719520009823E-4 1.0284152152385631E-5 6 0.0 1.0284152152385631E-5 0.0 2.1596719520009823E-4 1.0284152152385631E-5 7 0.0 1.0284152152385631E-5 0.0 2.2625134735248387E-4 1.0284152152385631E-5 8 0.0 1.0284152152385631E-5 0.0 2.4681965165725513E-4 1.0284152152385631E-5 9 0.0 1.0284152152385631E-5 0.0 3.0852456457156893E-4 2.0568304304771262E-5 10-11 0.0 1.0284152152385631E-5 0.0 3.7022947748588267E-4 2.0568304304771262E-5 12-13 3.599453253334971E-5 2.0568304304771262E-5 0.0 4.7821307508593184E-4 8.227321721908505E-5 14-15 5.142076076192815E-5 3.085245645715689E-5 0.0 5.861966726859809E-4 1.0798359760004912E-4 16-17 5.6562836838120966E-5 3.085245645715689E-5 0.0 7.044644224384158E-4 1.336939779810132E-4 18-19 6.68469889905066E-5 3.085245645715689E-5 0.0 9.050053894099355E-4 1.5426228228578446E-4 20-21 8.227321721908505E-5 5.142076076192815E-5 0.0 0.0012032458018291186 1.7997266266674852E-4 22-23 1.028415215238563E-4 5.142076076192815E-5 0.0 0.0020156938218675834 1.8511473874294133E-4 24-25 1.336939779810132E-4 5.142076076192815E-5 0.0 0.0038514149810684184 1.8511473874294133E-4 26-27 1.336939779810132E-4 5.142076076192815E-5 0.0 0.0072451851913556764 2.056830430477126E-4 28-29 1.8511473874294133E-4 5.142076076192815E-5 0.0 0.01936505850294214 2.056830430477126E-4 30-31 2.056830430477126E-4 5.142076076192815E-5 0.0 0.06389029524669573 2.056830430477126E-4 32-33 2.1082511912390542E-4 5.142076076192815E-5 0.0 0.1686652373752005 2.1596719520009823E-4 34-35 2.3139342342867668E-4 6.170491291431378E-5 0.0 0.2940136258845528 2.365354995048695E-4 36-37 2.673879559620264E-4 6.68469889905066E-5 0.0 0.4871140087738216 2.4167757558106231E-4 38-39 2.8795626026679766E-4 9.255736937147067E-5 0.0 0.8012382941923645 2.4681965165725513E-4 40-41 2.9309833634299045E-4 9.769944544766348E-5 0.0 1.156905412230469 2.4681965165725513E-4 42-43 3.0338248849537614E-4 1.028415215238563E-4 0.0 1.5050033942844179 2.519617277334479E-4 44-45 3.0852456457156893E-4 1.028415215238563E-4 0.0 1.8765338170175774 2.622458798858336E-4 46-47 3.959398578668468E-4 1.028415215238563E-4 0.0 2.283482859763553 2.8281418419060487E-4 48-49 4.1650816217161804E-4 1.028415215238563E-4 0.0 2.707997234385803 2.982404124191833E-4 50-51 4.2165023824781083E-4 1.028415215238563E-4 0.0 3.1474082034007838 2.982404124191833E-4 52-53 4.2165023824781083E-4 1.028415215238563E-4 0.0 3.5708684524274146 3.496611731811114E-4 54-55 4.267923143240036E-4 1.028415215238563E-4 0.0 3.9934545485210924 3.5994532533349704E-4 56-57 4.422185425525821E-4 1.028415215238563E-4 0.0 4.44328336366644 3.7022947748588267E-4 58-59 4.422185425525821E-4 1.028415215238563E-4 0.0 4.9133205377912255 3.805136296382683E-4 60-61 4.7821307508593184E-4 1.1312567367624193E-4 0.0 5.400671082064552 3.805136296382683E-4 62-63 4.936393033145103E-4 1.1312567367624193E-4 5.1420760761928155E-6 5.874600807779014 4.0108193394303957E-4 64-65 4.936393033145103E-4 1.1312567367624193E-4 1.0284152152385631E-5 6.332548961048671 4.0108193394303957E-4 66-67 5.604862923050168E-4 1.1312567367624193E-4 1.0284152152385631E-5 6.810828883123593 4.0108193394303957E-4 68-69 5.810545966097882E-4 1.1312567367624193E-4 1.0284152152385631E-5 7.320712004762797 4.0622401001923236E-4 70-71 6.11907053066945E-4 1.1312567367624193E-4 1.0284152152385631E-5 7.841403770314157 4.3193439040019647E-4 72-73 6.273332812955234E-4 1.1312567367624193E-4 1.0284152152385631E-5 8.35558052547698 4.5250269470496773E-4 74-75 6.376174334479091E-4 1.2340982582862757E-4 1.0284152152385631E-5 8.856146205266125 4.5250269470496773E-4 76-77 6.376174334479091E-4 1.2340982582862757E-4 1.0284152152385631E-5 9.358866414931189 4.5250269470496773E-4 78-79 6.427595095241019E-4 1.2340982582862757E-4 1.0284152152385631E-5 9.884499715591772 4.6278684685735336E-4 80-81 6.787540420574515E-4 1.2340982582862757E-4 1.0284152152385631E-5 10.430536774122686 4.6278684685735336E-4 82-83 6.787540420574515E-4 1.2855190190482038E-4 1.0284152152385631E-5 10.981242837730786 4.8335515116212463E-4 84-85 6.838961181336444E-4 1.336939779810132E-4 1.0284152152385631E-5 11.51701631241362 4.8335515116212463E-4 86-87 6.890381942098372E-4 1.336939779810132E-4 1.0284152152385631E-5 12.042443930031155 4.884972272383174E-4 88-89 6.890381942098372E-4 1.336939779810132E-4 1.0284152152385631E-5 12.580305087600923 4.936393033145103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8590 0.0 51.939014 1 GTATCAA 16525 0.0 45.314373 1 ATCAACG 20235 0.0 37.52238 3 TCAACGC 20200 0.0 37.515476 4 CAACGCA 21015 0.0 36.03647 5 TATCAAC 20980 0.0 34.877506 2 AACGCAG 22215 0.0 34.48343 6 ACGCAGA 24895 0.0 30.61907 7 CGCAGAG 26160 0.0 29.065828 8 GTACATG 27095 0.0 25.234394 1 GCAGAGT 31290 0.0 23.936323 9 ACATGGG 28905 0.0 23.177305 3 TACATGG 29345 0.0 22.57142 2 TACCTGG 8090 0.0 20.85019 2 AGAGTAC 28035 0.0 20.771248 10-11 CAGAGTA 29460 0.0 19.879387 10-11 CATGGGG 23975 0.0 19.698309 4 GAGTACT 15575 0.0 19.426779 12-13 TATAACG 565 0.0 19.342365 2 TATCACG 910 0.0 18.274979 2 >>END_MODULE