FastQCFastQC Report
Fri 27 May 2016
SRR940187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723699
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162180.16678837960739015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112320.11551159697559539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86850.052.4665951
GTATCAA177000.048.025421
TCAACGC214700.038.8740584
ATCAACG215450.038.672593
CAACGCA223150.037.358295
TATCAAC224950.037.2933652
AACGCAG233900.035.9841546
ACGCAGA269750.031.2015047
CGCAGAG277650.030.1768688
TATAACG8400.029.4070132
TAACGCA9250.027.2178824
TATCACG9100.027.1449382
ATAACGC8450.026.4217363
GTACATG278150.024.9577391
GCAGAGT334900.024.706229
TACATGG295050.023.0715872
ACATGGG286750.022.8444673
TACCTGG79150.021.5461852
AGAGTAC300650.021.3119910-11
CATGGGG237150.020.0107124
Produced by FastQC (version 0.11.5)