FastQCFastQC Report
Fri 27 May 2016
SRR940186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9890753
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127290.12869596480672402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80800.052.8696861
GTATCAA159300.046.2509571
ATCAACG198550.039.106773
TCAACGC202050.038.6404954
CAACGCA211850.036.8070685
TATCAAC201650.036.155312
AACGCAG222300.035.332636
TATAACG6550.032.9749222
ACGCAGA252800.031.0145387
CGCAGAG262600.029.785678
ATAACGC8600.027.6105883
GTACATG271350.026.0732841
TATCACG8850.026.0321792
TAACGCA9000.025.3278564
GCAGAGT312400.024.6396399
ACATGGG290700.024.4557443
TACATGG287700.023.9233442
ATCACGC12000.021.7663483
CATGGGG245250.021.3001844
TACCTGG80800.020.7313022
Produced by FastQC (version 0.11.5)