FastQCFastQC Report
Fri 27 May 2016
SRR940186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9890753
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161730.16351636725737667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114560.11582535728068429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.053.1831671
GTATCAA181150.046.845571
TCAACGC219700.037.94844
ATCAACG219700.037.710733
CAACGCA229850.036.3102875
TATCAAC234650.035.673792
AACGCAG240600.035.023576
TATAACG6850.033.9836042
ACGCAGA276150.030.4103977
TATCACG9400.030.3240592
CGCAGAG285400.029.5241858
ATAACGC7600.028.7536353
TAACGCA8100.026.3920924
GTACATG288950.025.175491
ATCACGC11400.024.5864453
GCAGAGT346100.024.2089129
ACATGGG296350.023.1959483
TACATGG306550.023.1688122
TACCTGG82700.021.7719652
AGAGTAC311600.021.302610-11
Produced by FastQC (version 0.11.5)