FastQCFastQC Report
Fri 27 May 2016
SRR940185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255125
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128040.13834497102956472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79550.052.3702741
GTATCAA156300.045.679931
ATCAACG187250.037.7963563
TCAACGC189100.037.526454
CAACGCA198000.035.839275
TATCAAC198600.035.4941372
AACGCAG207050.034.6422426
ACGCAGA240950.029.7878727
CGCAGAG250000.028.480938
TATCACG8100.024.6301022
TATAACG6000.024.5421372
GTACATG260650.024.348611
GCAGAGT292600.023.8465719
ACATGGG275150.022.3210433
TACATGG281100.022.1535742
TACCTGG73750.021.8987262
AGAGTAC266700.020.33895310-11
CATGGGG227950.019.6911324
ATAACGC8050.019.4716853
CAGAGTA280100.019.4252910-11
Produced by FastQC (version 0.11.5)