FastQCFastQC Report
Fri 27 May 2016
SRR940185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255125
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154790.1672478761767129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109620.11844248456936023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87750.051.3859941
GTATCAA167050.045.2247621
TCAACGC201200.036.615424
ATCAACG204000.036.0897673
TATCAAC213050.034.892472
CAACGCA211350.034.8341265
AACGCAG220650.033.624256
ACGCAGA253450.029.2727997
TATAACG6150.028.5775872
CGCAGAG261950.028.2685288
ATAACGC6700.026.939613
GTACATG253400.025.9510651
TACATGG272750.023.8243062
GCAGAGT310950.023.6611779
ACATGGG266750.023.5935883
TAACGCA7550.022.6483064
TACCTGG80200.022.4472852
AGAGTAC281400.021.0652210-11
CATGGGG222350.020.6357464
CAGAGTA295150.019.44015110-11
Produced by FastQC (version 0.11.5)