FastQCFastQC Report
Fri 27 May 2016
SRR940184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397063
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132260.14074610333037035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82850.050.2759971
GTATCAA160150.046.1758841
TCAACGC193350.037.5115134
ATCAACG194150.037.161833
TATCAAC202300.035.876012
CAACGCA202750.035.773155
AACGCAG212200.034.5376366
ACGCAGA243800.029.9251617
CGCAGAG253450.028.654598
TATAACG6700.028.3571052
TATCACG8500.025.704882
ATAACGC7450.025.5023613
GTACATG271350.025.0649451
GCAGAGT299250.024.0036669
TACATGG293350.022.8140092
ACATGGG282850.022.77093
TACCTGG80750.021.1756462
TAACGCA8600.020.9872194
AGAGTAC269400.020.98523910-11
CATGGGG232700.020.288964
Produced by FastQC (version 0.11.5)