##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9397063 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18906641362307 34.0 31.0 34.0 30.0 34.0 2 32.19326134133612 34.0 31.0 34.0 30.0 34.0 3 32.2984052570468 34.0 31.0 34.0 30.0 34.0 4 35.68089114652099 37.0 35.0 37.0 35.0 37.0 5 35.68714256784274 37.0 37.0 37.0 35.0 37.0 6 35.758744194861734 37.0 37.0 37.0 35.0 37.0 7 35.718348275413284 37.0 37.0 37.0 35.0 37.0 8 35.706118496811186 37.0 37.0 37.0 35.0 37.0 9 37.450176613693024 39.0 39.0 39.0 35.0 39.0 10-11 37.37581215535109 39.0 38.0 39.0 35.0 39.0 12-13 37.312920111315634 39.0 38.0 39.0 35.0 39.0 14-15 38.73876119591834 41.0 39.0 41.0 36.0 41.0 16-17 38.65529485116786 41.0 39.0 41.0 35.0 41.0 18-19 38.61091327151898 41.0 39.0 41.0 35.0 41.0 20-21 38.54040629503069 41.0 39.0 41.0 35.0 41.0 22-23 38.421504144433214 40.0 39.0 41.0 34.0 41.0 24-25 38.299642930988114 40.0 38.0 41.0 34.0 41.0 26-27 38.135581138489755 40.0 38.0 41.0 34.0 41.0 28-29 37.96689518842217 40.0 38.0 41.0 33.5 41.0 30-31 37.9141260412961 40.0 38.0 41.0 33.5 41.0 32-33 37.910898490304895 40.0 38.0 41.0 33.5 41.0 34-35 37.92633091850081 40.0 38.0 41.0 33.5 41.0 36-37 38.044208121197016 40.0 38.0 41.0 34.0 41.0 38-39 37.95514109035983 40.0 38.0 41.0 33.5 41.0 40-41 37.82495759579349 40.0 38.0 41.0 33.0 41.0 42-43 37.69493792901037 40.0 38.0 41.0 33.0 41.0 44-45 37.520074091234676 40.0 38.0 41.0 33.0 41.0 46-47 37.315749399573036 40.0 38.0 41.0 32.0 41.0 48-49 37.107375942887685 40.0 37.5 41.0 31.5 41.0 50-51 36.168383408731 39.0 36.0 40.0 30.0 40.5 52-53 36.24987184825727 39.0 36.0 40.5 30.5 41.0 54-55 36.49951160272097 40.0 36.0 41.0 30.5 41.0 56-57 36.430916500187344 40.0 36.0 41.0 31.0 41.0 58-59 36.1995806030033 39.0 35.5 41.0 30.0 41.0 60-61 35.89988382540375 39.0 35.0 41.0 30.0 41.0 62-63 35.553382902721836 38.5 35.0 41.0 29.0 41.0 64-65 35.160768848734975 37.5 35.0 40.0 29.0 41.0 66-67 34.766570310319295 37.0 35.0 40.0 28.0 41.0 68-69 34.34829243988254 36.5 34.5 39.0 28.0 41.0 70-71 33.9085435523844 36.0 34.0 39.0 26.5 41.0 72-73 33.464127515160854 35.5 34.0 38.5 26.0 40.0 74-75 33.009681801643765 35.0 34.0 37.0 26.0 39.5 76-77 32.53777786740389 35.0 33.0 37.0 25.0 39.0 78-79 32.08693280017384 35.0 33.0 36.0 23.5 39.0 80-81 31.62626993136047 35.0 33.0 36.0 21.0 37.0 82-83 31.201302204742056 35.0 32.0 35.0 19.5 37.0 84-85 30.794023302812803 35.0 32.0 35.0 16.5 36.5 86-87 30.427996864552256 35.0 31.5 35.0 9.0 36.0 88-89 30.0326889901664 34.5 31.0 35.0 4.0 36.0 90-91 29.663032747572302 34.0 31.0 35.0 2.0 35.5 92-93 29.242753187884343 34.0 30.5 35.0 2.0 35.0 94-95 28.787230914595337 34.0 30.0 35.0 2.0 35.0 96-97 28.312362969153234 34.0 29.0 35.0 2.0 35.0 98-99 27.744833731560597 34.0 29.0 35.0 2.0 35.0 100-101 25.724514936209324 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10732378306173729 1101 2 0.15248966227171223 1101 3 0.1720647506191355 1101 4 0.1423768987003058 1101 5 0.11561694763928898 1101 6 0.0609689752577367 1101 7 0.06322194743945886 1101 8 0.09922403167879423 1101 9 0.11722209159461272 1101 10-11 0.126788836985547 1101 12-13 0.18249044229542477 1101 14-15 0.1666547928510269 1101 16-17 0.20203052595950766 1101 18-19 0.18722997859820367 1101 20-21 0.11161234516190888 1101 22-23 0.14742228860212236 1101 24-25 0.1427267664824825 1101 26-27 0.15358459049540585 1101 28-29 0.09250968696935047 1101 30-31 0.16754607394201315 1101 32-33 0.11052945872889808 1101 34-35 0.08486646516410445 1101 36-37 0.10143475611932473 1101 38-39 0.1695309722950853 1101 40-41 0.1774861051937151 1101 42-43 0.154599467861253 1101 44-45 0.13306478547924172 1101 46-47 0.19062824470313444 1101 48-49 0.2240110749033235 1101 50-51 0.1458123029267 1101 52-53 0.22323146627078927 1101 54-55 0.3898357955452525 1101 56-57 0.30816649239229577 1101 58-59 0.30097661006693954 1101 60-61 0.25692773228417565 1101 62-63 0.21061653461702434 1101 64-65 0.23449937711801994 1101 66-67 0.16888792369977068 1101 68-69 0.23646090758952454 1101 70-71 0.20825044909147294 1101 72-73 0.12285036832489027 1101 74-75 0.15874169861523058 1101 76-77 0.10869271131962677 1101 78-79 0.11077818747951795 1101 80-81 0.00624536768977535 1101 82-83 -0.042304323212363215 1101 84-85 -0.010390967464861234 1101 86-87 -0.06879285216352216 1101 88-89 -0.05352829065705578 1101 90-91 -0.07790581230873528 1101 92-93 -0.0494058888242499 1101 94-95 -0.09942613221044994 1101 96-97 -0.041248326001944946 1101 98-99 -0.10187115132359992 1101 100-101 0.19618957507590196 1102 1 0.09591008642171062 1102 2 0.11068843901846037 1102 3 0.12411830456368733 1102 4 0.0877165691744608 1102 5 0.09216273769804673 1102 6 0.054195575420251885 1102 7 0.07350738380629451 1102 8 0.11324618493382843 1102 9 0.14245886531486462 1102 10-11 0.11606638043753748 1102 12-13 0.10485968586936423 1102 14-15 0.13725305161589318 1102 16-17 0.10072213029167898 1102 18-19 0.14550364879789868 1102 20-21 0.16045567857694465 1102 22-23 0.1291519360147788 1102 24-25 0.17803401316861311 1102 26-27 0.07317513027897604 1102 28-29 0.13411553863799952 1102 30-31 0.09623541519469114 1102 32-33 0.06847557635116885 1102 34-35 0.07809470506325766 1102 36-37 0.14839827722614274 1102 38-39 0.18782387429783398 1102 40-41 0.1762379012215476 1102 42-43 0.1720210563229685 1102 44-45 0.17794394016804205 1102 46-47 0.20908852737866823 1102 48-49 0.203662003879721 1102 50-51 0.16137866905676646 1102 52-53 0.18937372248573325 1102 54-55 0.34507770807869065 1102 56-57 0.28335713728419165 1102 58-59 0.23257528746265166 1102 60-61 0.18162107177352738 1102 62-63 0.189495007952722 1102 64-65 0.16746048132591085 1102 66-67 0.20406844910880295 1102 68-69 0.2501901431489202 1102 70-71 0.151732832380425 1102 72-73 0.16791741254071013 1102 74-75 0.12121277274985687 1102 76-77 0.021647734898188276 1102 78-79 0.13927699862187382 1102 80-81 0.12958398955841588 1102 82-83 0.11388932526410045 1102 84-85 0.054897922430274804 1102 86-87 0.06586470778640674 1102 88-89 0.07104162767701894 1102 90-91 0.012830423857263185 1102 92-93 0.04148495021489751 1102 94-95 0.02379693073646294 1102 96-97 0.05191718000243739 1102 98-99 0.01656585437930147 1102 100-101 0.2746001781037499 1103 1 0.061911354374494465 1103 2 0.13828283913176165 1103 3 0.051521306489725305 1103 4 0.053502912917636536 1103 5 0.04012169180429481 1103 6 0.016631211878497254 1103 7 0.01721551770688734 1103 8 0.03708706879562129 1103 9 0.040789557797317855 1103 10-11 -0.010784798868613166 1103 12-13 0.03611650201140293 1103 14-15 0.037965082003758255 1103 16-17 0.03372864635534967 1103 18-19 -0.0509096926416035 1103 20-21 -0.01606584207942774 1103 22-23 -0.062867692147492 1103 24-25 -0.061570741279723507 1103 26-27 -3.9400021985613876E-4 1103 28-29 0.036492330025005515 1103 30-31 -0.034128839574982806 1103 32-33 -0.022443477914372068 1103 34-35 -0.01895040465942799 1103 36-37 0.016874753332807302 1103 38-39 -0.0263669241864406 1103 40-41 -0.02112720754436026 1103 42-43 0.026914226984629863 1103 44-45 0.05360393281403475 1103 46-47 0.030317021084314888 1103 48-49 0.10304957575061024 1103 50-51 0.07183220264973045 1103 52-53 0.1378212760807358 1103 54-55 0.19522196580383877 1103 56-57 0.09875006757836502 1103 58-59 0.22238129050034416 1103 60-61 0.2442629570606769 1103 62-63 0.2539853414938378 1103 64-65 0.19931025049005058 1103 66-67 0.19878945028473538 1103 68-69 0.23724285002475654 1103 70-71 0.1348776973240433 1103 72-73 0.11095406243512684 1103 74-75 0.1458260796088382 1103 76-77 0.20854463044746296 1103 78-79 0.18319515072692383 1103 80-81 0.191158612223564 1103 82-83 0.1939872178085551 1103 84-85 0.19049490810252578 1103 86-87 0.09648937754431941 1103 88-89 0.16334532831265136 1103 90-91 0.08221274954847857 1103 92-93 0.21556605033088871 1103 94-95 0.32892959169851466 1103 96-97 0.1922826351553546 1103 98-99 0.07200777864458985 1103 100-101 0.2666562889351205 1104 1 0.08359333133526547 1104 2 0.09900403140821368 1104 3 0.062176420966736146 1104 4 0.08110171224235074 1104 5 0.10821506907691258 1104 6 0.13875919612012666 1104 7 0.13751687953396186 1104 8 0.09137010995065964 1104 9 0.14018866104000693 1104 10-11 0.21221084359714126 1104 12-13 0.1745612257994722 1104 14-15 0.15964082409885094 1104 16-17 0.14604282280374292 1104 18-19 0.2428036590139797 1104 20-21 0.23987098662274065 1104 22-23 0.20814147557477014 1104 24-25 0.21917149998562024 1104 26-27 0.2533561205642343 1104 28-29 0.24339564466048813 1104 30-31 0.31041465191756146 1104 32-33 0.25494830911991073 1104 34-35 0.29614782740456036 1104 36-37 0.2490993690741945 1104 38-39 0.20023937782096368 1104 40-41 0.19850096143932205 1104 42-43 0.22063825554756278 1104 44-45 0.18956663580862454 1104 46-47 0.16168769347667222 1104 48-49 0.19265616098532945 1104 50-51 0.09742955635000072 1104 52-53 0.1355188692705056 1104 54-55 0.3151107551460157 1104 56-57 0.1842414871031579 1104 58-59 0.20788199200563184 1104 60-61 0.237031810603348 1104 62-63 0.18372537983808712 1104 64-65 0.3132151237733467 1104 66-67 0.2754476098413363 1104 68-69 0.2751268509489293 1104 70-71 0.2301188609111975 1104 72-73 0.21514276137119026 1104 74-75 0.1699343031565661 1104 76-77 0.17515563267233603 1104 78-79 0.22397211560917185 1104 80-81 0.239034502791462 1104 82-83 0.2788973130005381 1104 84-85 0.2265397379873697 1104 86-87 0.23706434901434648 1104 88-89 0.2719638461518734 1104 90-91 0.28381029410218517 1104 92-93 0.2427430329218474 1104 94-95 0.2700942067536438 1104 96-97 0.2838716344100156 1104 98-99 0.36472039605412476 1104 100-101 0.5793495298959392 1105 1 -0.21116919082374963 1105 2 -0.039114267552143644 1105 3 -0.059763481203518154 1105 4 0.05338392191293906 1105 5 0.05910295934529586 1105 6 0.08625104766665004 1105 7 0.10207888909044982 1105 8 0.04131367590603929 1105 9 0.057892937253932075 1105 10-11 0.004248370224182452 1105 12-13 0.0668379463669524 1105 14-15 0.09295472887853151 1105 16-17 0.10151359758977918 1105 18-19 0.13964555298667136 1105 20-21 0.03772825732958296 1105 22-23 0.14871967027034572 1105 24-25 0.15386204123184655 1105 26-27 0.21381369721711962 1105 28-29 0.2712253705622416 1105 30-31 0.25175945601387895 1105 32-33 0.25388500504211464 1105 34-35 0.17138984573072946 1105 36-37 0.23934993837912089 1105 38-39 0.23267365751807034 1105 40-41 0.16505404553436875 1105 42-43 0.14874180598156528 1105 44-45 0.11371730410792935 1105 46-47 0.07776296664374627 1105 48-49 0.12944406021661337 1105 50-51 0.05772736400946599 1105 52-53 0.1103752448952946 1105 54-55 0.39358096250557395 1105 56-57 0.29071962166145227 1105 58-59 0.287564152675472 1105 60-61 0.3540481409198861 1105 62-63 0.36514203201384277 1105 64-65 0.3124382499779088 1105 66-67 0.32584196386071795 1105 68-69 0.2940080540647969 1105 70-71 0.32108466061891505 1105 72-73 0.3639066297089215 1105 74-75 0.25773999427369176 1105 76-77 0.1851769859677077 1105 78-79 0.24461582599061416 1105 80-81 0.380311166066182 1105 82-83 0.4018508241480738 1105 84-85 0.43720627557759073 1105 86-87 0.4365296813902084 1105 88-89 0.4760022850353849 1105 90-91 0.40738119517381577 1105 92-93 0.4066667078220334 1105 94-95 0.4876837095919839 1105 96-97 0.4319877221648234 1105 98-99 0.3771461502657516 1105 100-101 0.5335189068995092 1106 1 -0.1407106216063525 1106 2 -0.018370782192853596 1106 3 -0.0191577627377697 1106 4 0.10963856008269346 1106 5 0.09825653312135074 1106 6 0.14778445309039512 1106 7 0.11415668312390892 1106 8 0.1043005512108337 1106 9 -0.015809700406947513 1106 10-11 0.00848898256860764 1106 12-13 0.13215291784883476 1106 14-15 0.09886720263974524 1106 16-17 -0.010361442165965684 1106 18-19 0.15416461453916241 1106 20-21 0.13938236118217162 1106 22-23 0.2059124114021742 1106 24-25 0.1920850656365758 1106 26-27 0.2303567235301216 1106 28-29 0.21499140537766692 1106 30-31 0.17915049878045153 1106 32-33 0.24104416582493116 1106 34-35 0.02097479579752104 1106 36-37 0.12470392349457171 1106 38-39 0.08302297795994207 1106 40-41 0.23344518371471423 1106 42-43 0.2529930198568522 1106 44-45 0.20105364297526762 1106 46-47 0.16380273828676906 1106 48-49 0.15580953739151227 1106 50-51 0.07357201447699424 1106 52-53 0.20777005120532266 1106 54-55 0.3452776050007458 1106 56-57 0.28738358493774996 1106 58-59 0.32815078838567047 1106 60-61 0.39467758031093325 1106 62-63 0.4025022243256089 1106 64-65 0.21050029013650118 1106 66-67 0.31191420269200165 1106 68-69 0.3219853614879895 1106 70-71 0.31440291327459846 1106 72-73 0.3396801439043742 1106 74-75 0.44445389076034303 1106 76-77 0.19745024572641512 1106 78-79 0.08363131482720831 1106 80-81 0.27355414785274945 1106 82-83 0.46667295696405375 1106 84-85 0.365839155165407 1106 86-87 0.4447813829359113 1106 88-89 0.4927629077693112 1106 90-91 0.3348452559841988 1106 92-93 0.43986924476837075 1106 94-95 0.5059159573285577 1106 96-97 0.4903462341339484 1106 98-99 0.5679652245400746 1106 100-101 0.8827322733096175 1107 1 0.09617993568826222 1107 2 0.10500334077401874 1107 3 0.11119150388461208 1107 4 0.11000143617405911 1107 5 0.10357383321638736 1107 6 0.06802350843212679 1107 7 0.09142555263757401 1107 8 0.048639632487663675 1107 9 0.027902414734590764 1107 10-11 0.10511136568614887 1107 12-13 0.1932319475484121 1107 14-15 0.2145028410957437 1107 16-17 0.1582493558857081 1107 18-19 0.21962358623370193 1107 20-21 0.23955423511860374 1107 22-23 0.2664798366491752 1107 24-25 0.22720806137407834 1107 26-27 0.19703280352054264 1107 28-29 0.2728373461025839 1107 30-31 0.2345941355889991 1107 32-33 0.2236665885775153 1107 34-35 0.2685258345373356 1107 36-37 0.24380887796165496 1107 38-39 0.2858363123961638 1107 40-41 0.2684957593276138 1107 42-43 0.1993071112363154 1107 44-45 0.23738069329196065 1107 46-47 0.22446275660505677 1107 48-49 0.24236662176850388 1107 50-51 0.27528521785907145 1107 52-53 0.32484432340676506 1107 54-55 0.4662992802018806 1107 56-57 0.39885328911382345 1107 58-59 0.4101799886451971 1107 60-61 0.4747520941999994 1107 62-63 0.42878465505690855 1107 64-65 0.46043984668925475 1107 66-67 0.3696960703471319 1107 68-69 0.36420916983013996 1107 70-71 0.3969587749965342 1107 72-73 0.47121642634372307 1107 74-75 0.467500572229838 1107 76-77 0.4503211479148703 1107 78-79 0.4848525158725394 1107 80-81 0.5494562221443395 1107 82-83 0.6266152147314443 1107 84-85 0.5899182434559194 1107 86-87 0.6191362040655264 1107 88-89 0.6688023691979446 1107 90-91 0.6261830271503896 1107 92-93 0.6619038539194086 1107 94-95 0.6847889476808682 1107 96-97 0.7761287441353382 1107 98-99 0.7233792643078161 1107 100-101 0.8283559717075839 1108 1 0.2956516448192019 1108 2 0.3351855770597041 1108 3 0.28679461821461416 1108 4 0.21623892174634562 1108 5 0.2950618375215939 1108 6 0.29641314507351524 1108 7 0.34043757522758256 1108 8 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-1.677042368534174 2316 54-55 -1.6343534934485362 2316 56-57 -1.7085940824087729 2316 58-59 -1.7060338458422564 2316 60-61 -1.6878043172361927 2316 62-63 -1.8425013907693 2316 64-65 -1.568827841776688 2316 66-67 -1.5962753361945516 2316 68-69 -1.677181413091958 2316 70-71 -1.4667670285601417 2316 72-73 -1.4543858396712253 2316 74-75 -1.553082179253792 2316 76-77 -1.6874660405459352 2316 78-79 -1.5603796329751418 2316 80-81 -1.6287288924960244 2316 82-83 -1.5822427843462314 2316 84-85 -1.2030793918901814 2316 86-87 -1.2511911585297675 2316 88-89 -1.8060901089464565 2316 90-91 -1.4687478515945536 2316 92-93 -1.2605000884354673 2316 94-95 -1.0996105515688903 2316 96-97 -1.1336001275697853 2316 98-99 -0.938364159167179 2316 100-101 -1.08909763917271 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95173.0 3 19546.0 4 15003.0 5 12304.0 6 11165.0 7 11332.0 8 12285.0 9 14090.0 10 16123.0 11 18696.0 12 21709.0 13 25291.0 14 26753.0 15 30601.0 16 37144.0 17 46704.0 18 43736.0 19 34022.0 20 34365.0 21 37134.0 22 38842.0 23 42909.0 24 48734.0 25 56354.0 26 66260.0 27 79587.0 28 97278.0 29 117761.0 30 144369.0 31 178456.0 32 225047.0 33 295597.0 34 414993.0 35 634917.0 36 1148429.0 37 2289887.0 38 2530907.0 39 423499.0 40 61.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.94768534356407 18.477144868556092 13.998913033991212 24.57625675388863 2 15.530359845426348 20.197929728197387 39.632614525328776 24.639095901047483 3 18.666294969550002 25.147683609540906 29.324124356315757 26.861897064593336 4 13.5305113017614 17.098940133560223 36.81055274568666 32.55999581899172 5 14.92316797139363 36.07088056587602 33.6587938043807 15.34715765834965 6 31.687920868969886 35.38312700748325 18.582897775461614 14.34605434808525 7 28.59260954489571 31.26310467238984 22.03510546008058 18.10918032263387 8 26.55767227096427 34.33772719719009 20.598558955735278 18.50604157611036 9 26.952593860775004 16.76572705656842 20.068728800335002 36.21295028232158 10-11 25.47963677624306 25.637954927174768 27.743443696011905 21.13896460057027 12-13 26.529645775821358 22.869538425871497 27.69603372745507 22.90478207085207 14-15 23.519020107627885 24.179452339445987 25.13558873850205 27.165938814424077 16-17 23.684743810151268 27.05643793399684 25.808064320121225 23.45075393573066 18-19 23.9268138610502 26.09855888246439 27.100006403736586 22.87462085274882 20-21 24.279273563279084 25.631702850607084 26.866998922029495 23.222024664084334 22-23 24.202315073528982 25.58169876747092 26.81819357746858 23.397792581531515 24-25 24.12047071716226 25.76793501700231 26.664983891767662 23.44661037406777 26-27 23.79165168695702 26.04631198439751 26.49367818554225 23.66835814310322 28-29 23.771753830835593 25.9763999738784 26.502898204469872 23.748947990816138 30-31 23.874434723888086 25.684696744614204 26.573810445318998 23.867058086178712 32-33 23.64858060377434 25.896143370164626 26.667021161760264 23.788254864300768 34-35 24.13679246764414 25.82353270888048 26.400942848612402 23.638731974862978 36-37 23.960932547676695 25.644975950752936 26.402305023330726 23.99178647823965 38-39 23.89153032398474 25.666952900974778 26.648878843488514 23.79263793155197 40-41 24.032601222649625 25.259007997997323 26.937204313870346 23.77118646548271 42-43 23.642773608226577 25.53551362203687 26.805289424211665 24.01642334552489 44-45 23.549368174287366 25.71298958902284 26.640708276195447 24.096933960494347 46-47 23.747485925065163 25.647560885661093 26.551451780157166 24.053501409116585 48-49 23.712137380244407 25.78470417487391 26.381735146208136 24.121423298673555 50-51 23.31385892435932 25.958387906150946 26.51076389456178 24.216989274927954 52-53 23.62474559528574 25.747124907392426 26.38044362221219 24.24768587510964 54-55 23.38385691435739 25.57914089049701 26.629123641631917 24.407878553513687 56-57 23.377009743117227 25.896651141735212 26.55757436610344 24.168764749044122 58-59 23.59807006052003 25.626680032775205 26.368252114496944 24.406997792207818 60-61 23.49246045291459 25.382597249465768 26.776288682946692 24.348653614672948 62-63 23.58497329013631 25.44722091019765 26.663194938763564 24.304610860902482 64-65 23.461935902134826 25.524444021297445 26.38820318201458 24.625416894553144 66-67 23.37130171492342 25.949085807903778 26.145375076467616 24.53423740070519 68-69 23.36792479490441 25.59082681506982 26.488112201998764 24.553136188027004 70-71 23.461389365518702 25.641536753290723 26.230021179414088 24.667052701776488 72-73 23.53738373540218 25.802426170852748 26.079322229212586 24.58086786453249 74-75 23.54874312706244 25.90309316387885 26.164214492603666 24.383949216455044 76-77 23.76586093214752 25.800486887912832 26.129269837403456 24.304382342536197 78-79 23.73647954961193 25.842904913638897 26.08978702047407 24.3308285162751 80-81 23.82207366424619 25.948581415437644 26.11717269685539 24.112172223460774 82-83 23.83889259011092 25.90947486763807 26.112948950116838 24.138683592134168 84-85 23.88101018762247 25.917172109104776 26.017496811128595 24.18432089214416 86-87 24.05925718096786 25.90169088794661 26.004110404673376 24.034941526412155 88-89 24.036469818171444 25.721358939402794 25.966010441146587 24.276160801279175 90-91 24.209445592865446 25.765340946920173 26.07526293713953 23.949950523074854 92-93 24.40181075113659 25.637422757947746 26.010781190506645 23.94998530040902 94-95 24.381845413749836 25.541019713854435 25.937705736478822 24.139429135916906 96-97 24.682919632923547 25.404365144247027 25.755655882069423 24.157059340760004 98-99 24.538772727512335 25.25067998905479 25.855286274287216 24.35526100914566 100-101 24.623081165895833 25.113175494516764 26.064987074558175 24.19875626502923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 724.0 1 570.5 2 935.0 3 1322.5 4 901.0 5 691.0 6 978.5 7 1721.0 8 3075.0 9 3918.0 10 3586.0 11 3307.5 12 3580.0 13 3933.5 14 4172.5 15 4434.0 16 4797.5 17 5081.5 18 5430.5 19 5861.0 20 6552.0 21 7698.0 22 9166.0 23 11048.5 24 13746.5 25 17594.5 26 22387.0 27 28454.5 28 35377.0 29 43838.5 30 55596.5 31 70152.0 32 86178.0 33 102685.5 34 123077.5 35 142921.0 36 161534.5 37 180581.5 38 195936.5 39 203750.0 40 213729.5 41 237431.0 42 262964.0 43 289590.5 44 315108.5 45 334397.5 46 350616.0 47 370572.0 48 397700.0 49 415342.5 50 424558.5 51 430279.5 52 423250.0 53 418702.0 54 414408.5 55 391054.5 56 363356.0 57 331020.5 58 292202.5 59 253312.5 60 209405.5 61 165120.5 62 129309.0 63 100334.0 64 75236.0 65 56486.5 66 41812.5 67 31611.0 68 22653.0 69 15550.5 70 11478.5 71 8333.0 72 6077.5 73 4430.0 74 3374.0 75 2523.0 76 1768.0 77 1217.0 78 884.5 79 631.5 80 431.5 81 324.5 82 249.5 83 208.5 84 167.0 85 140.5 86 121.5 87 98.5 88 88.0 89 86.0 90 73.0 91 52.5 92 49.5 93 44.0 94 33.5 95 32.0 96 28.5 97 27.0 98 23.5 99 12.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022400616022261424 2 0.034308591950484954 3 0.024656640058707706 4 0.02341157018953688 5 0.041608745200495095 6 0.023571194531738268 7 0.026742398130139174 8 0.03975710283095899 9 0.026146467252587326 10-11 0.028610002933895408 12-13 0.026194354555247743 14-15 0.03421281734516412 16-17 0.04173112386284949 18-19 0.04373174895177355 20-21 0.037086055504789106 22-23 0.043630653535046006 24-25 0.01986790979266607 26-27 0.025651631791763024 28-29 0.027024401134694957 30-31 0.027019080323288244 32-33 0.03640499164472985 34-35 0.040720169695574036 36-37 0.029775260631965544 38-39 0.015930509351698503 40-41 0.017542715207932522 42-43 0.02022440415691584 44-45 0.02157589025422092 46-47 0.015536769307601749 48-49 0.025625027734729456 50-51 0.018532386129581125 52-53 0.027487311727078984 54-55 0.023858518347700766 56-57 0.02314020880779452 58-59 0.022953980408559567 60-61 0.01989983466110635 62-63 0.02261876928993665 64-65 0.01970296463905797 66-67 0.01857495262083483 68-69 0.02250171143898897 70-71 0.03481406903412268 72-73 0.0253536663529871 74-75 0.033563678353545144 76-77 0.04188542739364416 78-79 0.02658277378793778 80-81 0.022906093105899153 82-83 0.016148662619373734 84-85 0.032621894734556955 86-87 0.020016892512054033 88-89 0.030690440193920164 90-91 0.024257579203204236 92-93 0.04197588118755828 94-95 0.07467226728180922 96-97 0.09394424619692344 98-99 0.07081467901194234 100-101 0.0791949569775152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9397063.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.978075549403 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63045936355935 34.77342974453786 2 12.634348817581387 11.618060889045351 3 4.123579501835668 5.6878274960810975 4 2.1119928567331954 3.8842146850671324 5 1.1929853352862132 2.742558493755969 6 0.8022268478857423 2.2130907971910054 7 0.5683380125997642 1.8291761656636658 8 0.42001871503962956 1.5449321769804205 9 0.3206606038619626 1.3269021723074252 >10 1.931948287677559 16.413396802324744 >50 0.155997342550836 4.95804042578355 >100 0.09749193036730144 8.52552817526189 >500 0.007123208332411254 2.2216558409544547 >1k 0.0027364168511777743 1.900789832472904 >5k 6.956987817885178E-5 0.2193767617948349 >10k+ 2.3189959392950593E-5 0.14101954077756917 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13226 0.14074610333037035 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.320811406712927E-5 0.0 2 0.0 0.0 0.0 5.320811406712927E-5 0.0 3 0.0 0.0 0.0 6.384973688055513E-5 0.0 4 0.0 1.0641622813425855E-5 0.0 8.513298250740684E-5 0.0 5 0.0 1.0641622813425855E-5 0.0 1.2769947376111026E-4 0.0 6 0.0 1.0641622813425855E-5 0.0 1.2769947376111026E-4 0.0 7 3.1924868440277564E-5 1.0641622813425855E-5 0.0 1.596243422013878E-4 0.0 8 3.1924868440277564E-5 1.0641622813425855E-5 0.0 1.7026596501481367E-4 0.0 9 3.1924868440277564E-5 1.0641622813425855E-5 0.0 2.553989475222205E-4 0.0 10-11 3.1924868440277564E-5 2.128324562685171E-5 0.0 3.618151756564791E-4 0.0 12-13 3.724567984699049E-5 2.128324562685171E-5 0.0 4.948354608243022E-4 1.5962434220138782E-5 14-15 5.320811406712927E-5 2.128324562685171E-5 0.0 6.651014258391159E-4 2.6604057033564637E-5 16-17 5.85289254738422E-5 2.6604057033564637E-5 0.0 8.619714478874942E-4 3.1924868440277564E-5 18-19 6.917054828726806E-5 3.1924868440277564E-5 0.0 0.0010641622813425855 3.1924868440277564E-5 20-21 7.449135969398098E-5 3.1924868440277564E-5 0.0 0.0014366190798124905 4.256649125370342E-5 22-23 7.449135969398098E-5 3.1924868440277564E-5 0.0 0.002303911339106697 5.320811406712927E-5 24-25 9.045379391411977E-5 3.1924868440277564E-5 0.0 0.003990608555034696 5.320811406712927E-5 26-27 9.577460532083269E-5 3.1924868440277564E-5 0.0 0.007236303513129582 8.513298250740684E-5 28-29 1.3834109657453612E-4 3.1924868440277564E-5 0.0 0.01928794134933436 8.513298250740684E-5 30-31 1.4898271938796197E-4 3.724567984699049E-5 0.0 0.06480748293376346 8.513298250740684E-5 32-33 1.4898271938796197E-4 4.256649125370342E-5 0.0 0.1718462460026074 1.0641622813425855E-4 34-35 1.596243422013878E-4 4.256649125370342E-5 0.0 0.2977260022626218 1.2769947376111026E-4 36-37 1.8090758782823952E-4 4.256649125370342E-5 0.0 0.4902755254487493 1.2769947376111026E-4 38-39 1.9154921064166538E-4 4.256649125370342E-5 0.0 0.8076938507276157 1.383410965745361E-4 40-41 1.968700220483783E-4 4.7887302660416346E-5 0.0 1.16666771309291 1.383410965745361E-4 42-43 2.500781361155076E-4 5.320811406712927E-5 0.0 1.5166494041808596 1.383410965745361E-4 44-45 3.086070615893498E-4 5.320811406712927E-5 0.0 1.8865841380439825 1.383410965745361E-4 46-47 3.724567984699049E-4 5.320811406712927E-5 0.0 2.292029967235507 1.4898271938796194E-4 48-49 3.8309842128333077E-4 5.320811406712927E-5 0.0 2.718226960913213 1.8090758782823952E-4 50-51 3.8309842128333077E-4 5.320811406712927E-5 0.0 3.162450863636862 1.8090758782823952E-4 52-53 3.884192326900437E-4 5.320811406712927E-5 0.0 3.5887010654286344 2.4475732470879464E-4 54-55 4.0438166691018245E-4 5.320811406712927E-5 0.0 4.007188203378012 2.660405703356464E-4 56-57 4.522689695705988E-4 5.320811406712927E-5 0.0 4.457775796544091 2.7668219314907225E-4 58-59 4.5758978097731174E-4 5.320811406712927E-5 0.0 4.925714555707459 2.92644627369211E-4 60-61 4.682314037907376E-4 5.320811406712927E-5 0.0 5.409328425275003 3.086070615893498E-4 62-63 4.948354608243022E-4 5.320811406712927E-5 0.0 5.885296288851102 3.4053193002962735E-4 64-65 5.214395178578669E-4 5.320811406712927E-5 0.0 6.344099214829145 3.4053193002962735E-4 66-67 5.746476319249961E-4 5.320811406712927E-5 0.0 6.822817937902513 3.511735528430532E-4 68-69 5.746476319249961E-4 5.85289254738422E-5 0.0 7.328044943404125 3.6181517565647903E-4 70-71 5.906100661451349E-4 6.384973688055513E-5 0.0 7.8509370427760246 3.9374004409675664E-4 72-73 5.959308775518479E-4 6.384973688055513E-5 0.0 8.363730242097983 4.309857239437471E-4 74-75 5.959308775518479E-4 6.384973688055513E-5 0.0 8.858932838909348 4.4694815816388587E-4 76-77 5.959308775518479E-4 6.384973688055513E-5 0.0 9.365899749740958 4.788730266041634E-4 78-79 5.959308775518479E-4 6.384973688055513E-5 0.0 9.892622833325689 4.948354608243022E-4 80-81 6.172141231786996E-4 6.917054828726806E-5 0.0 10.434707099441603 5.001562722310152E-4 82-83 6.172141231786996E-4 9.577460532083269E-5 0.0 10.977456466983355 5.533643862981444E-4 84-85 6.172141231786996E-4 1.170578509476844E-4 0.0 11.510905056186171 5.533643862981444E-4 86-87 6.225349345854125E-4 1.170578509476844E-4 0.0 12.03278088057939 5.640060091115703E-4 88-89 6.331765573988383E-4 1.170578509476844E-4 0.0 12.56991679208706 5.746476319249961E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8285 0.0 50.275997 1 GTATCAA 16015 0.0 46.175884 1 TCAACGC 19335 0.0 37.511513 4 ATCAACG 19415 0.0 37.16183 3 TATCAAC 20230 0.0 35.87601 2 CAACGCA 20275 0.0 35.77315 5 AACGCAG 21220 0.0 34.537636 6 ACGCAGA 24380 0.0 29.925161 7 CGCAGAG 25345 0.0 28.65459 8 TATAACG 670 0.0 28.357105 2 TATCACG 850 0.0 25.70488 2 ATAACGC 745 0.0 25.502361 3 GTACATG 27135 0.0 25.064945 1 GCAGAGT 29925 0.0 24.003666 9 TACATGG 29335 0.0 22.814009 2 ACATGGG 28285 0.0 22.7709 3 TACCTGG 8075 0.0 21.175646 2 TAACGCA 860 0.0 20.987219 4 AGAGTAC 26940 0.0 20.985239 10-11 CATGGGG 23270 0.0 20.28896 4 >>END_MODULE