FastQCFastQC Report
Fri 27 May 2016
SRR940184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397063
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155450.1654240266347049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107740.11465284419185015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86450.054.0425151
GTATCAA171400.046.113541
TCAACGC210800.036.7733354
ATCAACG213450.036.27193
CAACGCA219900.035.315995
TATCAAC222900.034.9474072
AACGCAG230700.033.9509546
ACGCAGA264900.029.4956677
TATAACG6200.029.112072
CGCAGAG273450.028.6255328
TATCACG7650.024.8358922
GTACATG267600.024.4565321
ATAACGC8800.024.288953
GCAGAGT330150.023.5942869
TACCTGG79900.022.8278832
TACATGG287550.022.2502782
ACATGGG280200.022.2065983
TAACGCA10050.021.740794
AGAGTAC295750.021.1671610-11
CATGGGG227100.019.9533024
Produced by FastQC (version 0.11.5)