FastQCFastQC Report
Fri 27 May 2016
SRR940183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808776
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.072.174911
GTATCAA44650.058.9244161
TCAACGC55700.045.4180224
ATCAACG56350.044.8941233
CAACGCA59900.042.229955
TATCAAC62400.041.972282
AACGCAG61000.041.5545046
GTACATG127800.039.9157681
TACATGG138200.036.2659072
ACATGGG134950.035.59553
ACGCAGA73700.034.551487
CGCAGAG74750.033.8301628
CATGGGA89000.031.9692464
CATGGGG48900.031.1620224
GCAGAGT80800.029.6770049
ATGGGAT38800.026.8450855
ATGGGAG39150.026.605095
TATAGGG3950.025.6180652
GAGTACA95250.025.4345441
GTATAAA12000.025.297841
Produced by FastQC (version 0.11.5)