FastQCFastQC Report
Fri 27 May 2016
SRR940183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808776
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84740.468493611149197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.37406511364591305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.21246411938238896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.086.628321
GTATCAA93700.063.1440351
ATCAACG125150.047.0751723
TCAACGC125800.046.9739954
TATCAAC128200.046.0958822
CAACGCA130750.045.107625
AACGCAG133700.044.1556436
ACGCAGA151150.039.0067147
CGCAGAG153600.038.307078
GTATCGC1156.649625E-736.2248156
GTACATG116250.035.980471
GCAGAGT166250.034.962859
TACATGG124250.033.7531852
GTGGTAT16100.033.6773721
ACATGGG118650.033.5880553
TGGTATC16300.031.795832
GAGTACT100950.030.3216612-13
CATGGGA74700.030.1437764
CATGGGG46050.029.3645064
CAGAGTA167050.027.99303210-11
Produced by FastQC (version 0.11.5)