FastQCFastQC Report
Fri 27 May 2016
SRR940182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786553
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC21400.11978373997300948No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG20380.1140744215256978No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG20280.11351468442302019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.068.492271
GTATCAA48800.062.93831
TCAACGC62500.046.1911774
ATCAACG63700.045.414463
CAACGCA63850.045.213285
TATCAAC69000.044.5128862
AACGCAG65800.043.4076966
GTACATG145000.041.9534841
TACATGG149500.040.0934522
ACATGGG155150.036.908013
ACGCAGA78650.036.1562427
CGCAGAG81200.034.660298
CATGGGA103050.034.2534264
ATGGGAG41600.033.6247985
CATGGGG55300.029.9238194
GCAGAGT89200.029.7506949
ATGGGAT40300.026.5859765
CAACGCG450.00953506826.4424734-35
TAACGTC1608.7186345E-626.0413325
ACTTGAC13450.024.7738118
Produced by FastQC (version 0.11.5)