FastQCFastQC Report
Fri 27 May 2016
SRR940182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786553
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87560.4901058071045191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71860.4022270819841337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.2341380300500461No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG25450.14245309263145287No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC22810.12767603312076384No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG20330.11379455297435899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48100.092.623171
GTATCAA104700.065.193011
TATCAAC139000.048.8806082
ATCAACG141050.048.08443
TCAACGC142000.047.7627144
CAACGCA144500.046.942075
AACGCAG148400.045.7873276
ACGCAGA169850.039.9281737
CGCAGAG172700.039.2692578
GTACATG136200.038.570441
TACATGG138800.037.367542
GTGGTAT13900.037.279281
TGGTATC13950.036.7104762
GCAGAGT185600.036.027039
ACATGGG144100.035.0417253
ATGGGAG36750.032.219665
CATGGGG52850.031.547624
CATGGGA93750.031.5038784
GCGTATC953.2672982E-431.3479771
GAGTACT114050.030.72275512-13
Produced by FastQC (version 0.11.5)