FastQCFastQC Report
Fri 27 May 2016
SRR940181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886106
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.061.450041
GTATCAA36150.056.8031
ATCAACG42600.047.7833943
TCAACGC43600.046.5509344
CAACGCA44750.045.487665
TATCAAC45100.045.3985942
AACGCAG45900.044.076956
GTACATG102950.038.3307841
TACATGG105350.036.949092
ACATGGG105650.035.999123
CATGGGG56700.034.6410754
ACGCAGA59150.034.198887
CGCAGAG58700.034.1597028
ATGGGGA33750.029.8039235
GCAGAGT67200.028.0680549
CATGGGA54400.025.9304434
GAGTACA73900.023.3568381
TAAGGTG10100.022.3935385
CAGAGTA65250.022.2494910-11
AGAGTAC59150.022.12986210-11
Produced by FastQC (version 0.11.5)