FastQCFastQC Report
Fri 27 May 2016
SRR940181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51230.2716178199952707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38120.20210953148974659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.11277202871948873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34700.078.2793661
GTATCAA73350.062.9380871
ATCAACG91450.050.0106743
TCAACGC93650.048.835844
TATCAAC94300.048.6280982
CAACGCA95350.047.8709265
AACGCAG97450.046.838096
ACGCAGA114600.039.818177
ACGTACG300.001309409239.6634830-31
CGCAGAG115500.039.4563878
TGGTATC14700.036.4614072
GTACATG94900.036.154991
GCAGAGT125600.035.7624979
TACATGG99250.033.782062
ACATGGG97300.033.539223
GTGGTAT18750.031.1341321
GAGTACT73100.029.82820512-13
CATGGGG54900.029.5040594
CAGAGTA122900.028.43979810-11
AGAGTAC116300.028.05869310-11
Produced by FastQC (version 0.11.5)