FastQCFastQC Report
Fri 27 May 2016
SRR940180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556139
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.061.8701441
GTATCAA59400.058.0208131
ATCAACG74350.045.713753
TCAACGC75100.045.3364874
TATCAAC76900.044.429232
CAACGCA78450.043.400515
AACGCAG81900.041.414766
GTACATG181150.038.3135071
TACATGG186850.036.506882
ACATGGG188050.034.881893
ACGCAGA100950.033.5346457
CGCAGAG102200.032.77848
CATGGGG87150.031.1450614
CATGGGA110700.030.6491874
ATGGGAG46050.029.2126225
GCAGAGT112700.028.5107489
ATGGGGA48950.027.3603465
GTGGTAT22200.024.131331
CAGAGTA109600.022.9366210-11
GAGTACA136650.022.7380161
Produced by FastQC (version 0.11.5)