FastQCFastQC Report
Fri 27 May 2016
SRR940180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556139
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60380.23621563616063132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.18301039184488793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.077.179021
GTATCAA99100.060.842411
ATCAACG129950.046.2940863
TCAACGC132150.045.52254
TATCAAC132750.045.2745442
CAACGCA134400.044.76445
AACGCAG138500.043.4822126
GTACATG169200.036.8676831
ACGCAGA164450.036.5752837
CGCAGAG165300.036.1712578
TACATGG175950.034.666352
ACATGGG173450.033.9283753
GCAGAGT180600.032.612789
CATGGGG80500.031.7419324
GAGTACT87050.029.55886712-13
GTGGTAT23200.029.5331231
TGGTATC21750.027.3866022
CATGGGA102750.027.3030414
CAGAGTA175000.027.28207410-11
AGTACTT91700.026.82727612-13
Produced by FastQC (version 0.11.5)