FastQCFastQC Report
Fri 27 May 2016
SRR940179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866832
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG24030.12872074187714802No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG23860.12781010824755523No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC20060.10745476829195127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40050.061.5376471
GGTATCA24150.054.724141
ATCAACG50900.047.368753
TCAACGC53200.045.3208544
CAACGCA53650.044.7163935
AACGCAG55400.042.748276
TATCAAC57800.042.5368462
GTACATG135050.042.3615531
TACATGG141550.039.4910622
ACATGGG142300.038.1125683
CATGGGG61750.034.7072534
ACGCAGA70300.033.4294177
CGCAGAG70700.033.157918
CATGGGA88700.031.9475864
ATGGGAG37450.028.9301725
GCAGAGT79850.028.0171059
AGTACAT92700.026.5224342
ATGGGGA30300.026.3265295
GAGTACA99350.026.0054841
ATGGGGG38500.025.8218385
Produced by FastQC (version 0.11.5)