FastQCFastQC Report
Fri 27 May 2016
SRR940179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866832
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61270.32820307344206656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.26820838725712864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.14291591316197708No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG26330.14104107921869777No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG24280.13005990897949038No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC21420.11473983732869374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.079.14631
GTATCAA84150.063.211281
ATCAACG111500.047.5330733
TATCAAC112300.047.1414342
TCAACGC113950.046.5620964
CAACGCA116150.045.6471185
AACGCAG119200.044.37936
TGGTATC13550.039.1139342
ACGCAGA139050.038.120357
GTACATG132000.037.7250331
CGCAGAG140300.037.6959158
TACATGG136150.035.9967232
ACATGGG135650.034.7246173
GTGGTAT17300.034.431121
GCAGAGT153750.034.088749
CGTATAC902.3818173E-433.083263
CATGGGA83150.030.5798114
CATGGGG59300.030.3264394
GAGTACT83950.029.90487312-13
ATGGGAG37450.028.4417365
Produced by FastQC (version 0.11.5)