FastQCFastQC Report
Fri 27 May 2016
SRR940178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557689
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG16770.1076594878695298No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG16230.10419281384153062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.059.68841
GTATCAA31400.056.8738371
TCAACGC39550.043.7993244
GTACATG100700.043.0942151
ATCAACG40500.042.9189153
CAACGCA41200.041.898055
TATCAAC43300.041.2433782
TACATGG106700.040.391972
AACGCAG42500.040.3208056
ACATGGG107550.037.748083
CATGGGG48750.035.6557164
ACGCAGA52850.032.4203267
CATGGGA62500.031.1450214
ATGGGGA24300.031.1093165
CGCAGAG54950.031.075068
CTTACGG1203.68502E-529.7639733
ATGGGAG29300.027.6289445
GCAGAGT61200.026.5404939
CGCATAA450.00953272626.44369934-35
GAGTACA74100.026.0284141
Produced by FastQC (version 0.11.5)