FastQCFastQC Report
Fri 27 May 2016
SRR940178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557689
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.3098821395028148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.26577834214660306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.14046449580115158No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG18650.11972864930034173No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG16290.10457799984464164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.079.933841
GTATCAA66700.064.859651
TCAACGC88700.048.4259074
ATCAACG89000.048.3311653
TATCAAC90650.047.845782
CAACGCA91400.047.0758065
AACGCAG92550.046.489366
ACGCAGA108400.039.6254467
CGCAGAG110100.039.0136078
GTACATG97500.038.4423451
TACATGG103900.035.7233772
GCAGAGT119300.035.6559339
ACATGGG103200.034.1760673
GTGGTAT13950.033.7455671
TGGTATC13850.030.541382
GAGTACT68850.030.11376612-13
CATGGGA62000.029.883734
CATGGGG46150.029.303554
CAGAGTA117150.028.7943310-11
GTACTTT72800.028.31640414-15
Produced by FastQC (version 0.11.5)