FastQCFastQC Report
Fri 27 May 2016
SRR940177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528727
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18840.12323979363221818No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18740.12258565460020003No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17670.11558636695760591No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16900.1105494964110662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.078.435411
GTATCAA41650.057.454511
TCAACGC53800.042.3769344
ATCAACG55300.041.5504043
GTACATG126850.041.2488141
CAACGCA56750.040.1740845
TATCAAC59500.039.8179782
TACATGG127700.039.762272
AACGCAG59100.038.2606936
ACATGGG134500.036.3799933
CATGGGA96700.033.980184
ACGCAGA71550.031.5169127
ATGGGAG40150.030.2452855
CGCAGAG73500.030.1960288
CATGGGG44150.028.5836874
ATGGGAT36900.028.392225
GCAGAGT79850.026.5287979
TAAGGTA5400.025.3540384
GAGTACA88900.024.9758431
CCTATAC3850.024.738443
Produced by FastQC (version 0.11.5)