FastQCFastQC Report
Fri 27 May 2016
SRR940177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528727
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.5807446326257075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69800.45658904434866393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.27100980096511673No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18700.12232399898739277No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18460.12075406531054925No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16890.11048408250786439No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16470.10773669857338819No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.10263441412364667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.091.274171
GTATCAA94900.063.8447271
TATCAAC130250.046.597992
ATCAACG130600.046.2861373
TCAACGC130800.046.2153634
CAACGCA132850.045.4694635
AACGCAG136050.044.4831126
TGGTATC13350.041.0466082
ACGCAGA157900.038.35657
GTACATG119900.038.0112841
CGCAGAG160650.037.6628768
TACATGG120900.037.244852
GCAGAGT169900.035.1921659
ACATGGG124250.034.942923
GTGGTAT16750.034.8563231
CATGGGA86500.032.4289474
ATGGGAG34000.030.1068175
ATGGGAT33950.029.6252675
AGAGTAC162550.029.02414710-11
CAGAGTA167150.028.77709210-11