FastQCFastQC Report
Fri 27 May 2016
SRR940176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197677
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27110.12335752706152905No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26280.11958081192095107No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26230.11935329896067529No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22890.10415543321425305No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22760.10356389951753603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.063.6555141
GTATCAA47850.058.0886081
ATCAACG59850.045.646193
TCAACGC60650.045.0440984
TATCAAC61800.044.7837562
CAACGCA61300.044.6635635
AACGCAG63600.043.1291926
GTACATG140000.039.707711
TACATGG143250.038.1420562
ACATGGG145650.036.4102133
ACGCAGA78600.034.7430767
CATGGGA76250.034.0331884
CGCAGAG80100.033.9477658
CATGGGG67900.029.3650684
ATGGGAG36050.027.7370365
GCAGAGT94350.027.6222929
ACCGTAT1358.2481856E-526.4450878
TAAGGTA7650.024.8968584
ATGGGAT33500.024.3406285
CGTATAG1751.7473603E-523.8076231
Produced by FastQC (version 0.11.5)