FastQCFastQC Report
Fri 27 May 2016
SRR940176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197677
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.31196577113015245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.2409817275241084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.13782735133506882No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26500.12058186894616452No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25550.11625912270092466No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25540.1162136201088695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.076.596641
GTATCAA89800.063.2903181
ATCAACG117450.048.0730673
TATCAAC120000.047.2014852
CAACGCA121150.046.758935
TCAACGC121000.046.711884
AACGCAG125750.045.093736
GTACATG131550.038.9468961
ACGCAGA148350.038.057547
CGCAGAG149150.037.89338
TACATGG135650.037.1455652
ACATGGG135300.036.008253
TGGTATC17000.034.6850782
GCAGAGT166000.033.6168379
CATGGGA71750.031.7093984
GTGGTAT20000.031.5748351
CATGGGG66350.030.3404674
AGGTAAG10000.029.1845152
ATGGGAG35350.028.9527665
GAGTACT96100.028.75573212-13
Produced by FastQC (version 0.11.5)