FastQCFastQC Report
Fri 27 May 2016
SRR940175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252754
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55242.185524264700064No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14200.5618110890431012No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13880.5491505574590314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.29752249222564237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.23421983430529292No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.23382419269329072No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5330.21087697919716406No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT4030.15944356963688014No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3890.15390458706884955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.1404527722607753No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3250.1285835239007098No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC2870.11354914264462679No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2740.10840580168859841No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.10682323524058968No Hit
CTCCCGACCCGGGGAGGAAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA2600.10286681912056782No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT2550.10088861106055691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT900.085.942876
GGTATCA5400.084.8410341
CGTCACT551.9845174E-975.725881
CTAGATA1050.073.665324
TAGTCAA1150.072.433469
GTATCAA13450.068.567521
GTTATAT359.139467E-567.998761
GTCACTA753.2196112E-1063.4655082
ACAGTAC300.00423304159.4989138
CGTCTAG300.00423304159.4989131
ACTTAAG300.00423304159.4989138
GGAACGA300.00423304159.4989132
GATAGTC1300.059.4989137
TCACTAC752.3033863E-855.532323
GTATAGA551.3079127E-554.089921
GGTACTC453.1664773E-452.8879248
GTTCGAC800.052.0615516-17
TCGACCG800.052.0615518-19
TAGGCAT350.00778982250.999075
CGATCCG551.7644197E-1048.68092770-71