##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940175_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252754 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.606225816406464 34.0 31.0 34.0 31.0 34.0 2 32.644104544339555 34.0 31.0 34.0 31.0 34.0 3 32.683577707969015 34.0 31.0 34.0 31.0 34.0 4 36.16616156420868 37.0 35.0 37.0 35.0 37.0 5 36.195878205686164 37.0 37.0 37.0 35.0 37.0 6 36.311251256162116 37.0 37.0 37.0 35.0 37.0 7 36.2542788640338 37.0 37.0 37.0 35.0 37.0 8 36.28221116184116 37.0 37.0 37.0 35.0 37.0 9 38.036889623903086 39.0 39.0 39.0 35.0 39.0 10-11 38.025487232645176 39.0 39.0 39.0 35.0 39.0 12-13 38.02286215054954 39.0 39.0 39.0 35.0 39.0 14-15 39.21257823812877 41.0 38.5 41.0 36.0 41.0 16-17 39.24967953029428 41.0 39.0 41.0 36.0 41.0 18-19 39.51245084152971 41.0 39.5 41.0 36.0 41.0 20-21 39.527706782088515 41.0 40.0 41.0 36.0 41.0 22-23 39.38390292537408 41.0 39.0 41.0 35.0 41.0 24-25 39.28572050293962 41.0 39.0 41.0 35.0 41.0 26-27 39.02662668048775 41.0 38.5 41.0 35.0 41.0 28-29 38.784919328675315 41.0 38.0 41.0 35.0 41.0 30-31 38.141036343638476 40.0 36.5 41.0 35.0 41.0 32-33 37.35977867808224 40.0 35.0 41.0 33.5 41.0 34-35 36.9056137588327 39.5 35.0 41.0 33.0 41.0 36-37 36.547415668990396 39.0 35.0 41.0 33.0 41.0 38-39 36.17208036272423 39.0 35.0 41.0 32.5 41.0 40-41 35.82632915799552 39.0 35.0 41.0 31.0 41.0 42-43 35.29467387261923 39.0 35.0 41.0 28.0 41.0 44-45 34.66573822768383 39.0 35.0 41.0 20.5 41.0 46-47 33.24672606566068 39.0 34.0 41.0 2.0 41.0 48-49 31.953557609375125 39.0 32.5 41.0 2.0 41.0 50-51 31.497624172119927 39.0 32.0 41.0 2.0 41.0 52-53 31.426867626229456 39.0 32.5 41.0 2.0 41.0 54-55 31.263311362035815 38.0 32.0 41.0 2.0 41.0 56-57 31.116421500747762 38.0 32.0 41.0 2.0 41.0 58-59 30.917451355863804 37.5 31.5 41.0 2.0 41.0 60-61 30.669918181314635 37.0 31.0 41.0 2.0 41.0 62-63 30.40193627004914 36.5 31.0 40.5 2.0 41.0 64-65 30.082295433504513 36.0 30.5 40.0 2.0 41.0 66-67 29.718083591159782 35.5 29.0 40.0 2.0 41.0 68-69 29.291926141623872 35.0 28.5 39.0 2.0 41.0 70-71 28.92797740095112 35.0 27.5 39.0 2.0 41.0 72-73 28.65585707842408 35.0 26.0 39.0 2.0 41.0 74-75 28.355861826123423 35.0 26.0 37.0 2.0 40.5 76-77 28.04877074151151 35.0 26.0 37.0 2.0 39.5 78-79 27.743824034436646 35.0 25.0 36.5 2.0 39.0 80-81 27.446968198327227 35.0 24.5 36.0 2.0 39.0 82-83 27.153815567706147 35.0 23.5 36.0 2.0 37.0 84-85 26.900527785910413 35.0 21.5 35.0 2.0 37.0 86-87 26.698418224835216 35.0 20.0 35.0 2.0 36.5 88-89 26.496168210987758 35.0 19.0 35.0 2.0 36.0 90-91 26.340659297182242 35.0 17.5 35.0 2.0 36.0 92-93 26.183354170458234 35.0 11.0 35.0 2.0 36.0 94-95 26.036565197781243 35.0 7.0 35.0 2.0 35.5 96-97 25.924826115511525 35.0 2.0 35.0 2.0 35.0 98-99 25.819057265166922 35.0 2.0 35.0 2.0 35.0 100-101 25.733519548652048 35.0 2.0 35.0 2.0 35.0 102-103 25.046962659344658 33.5 2.0 35.0 2.0 35.0 104-105 25.478860868670722 35.0 2.0 35.0 2.0 35.0 106-107 25.45441219525705 35.0 2.0 35.0 2.0 35.0 108-109 25.36588936277962 35.0 2.0 35.0 2.0 35.0 110-111 25.241762741638112 35.0 2.0 35.0 2.0 35.0 112-113 25.086160852053776 35.0 2.0 35.0 2.0 35.0 114-115 24.890769285550377 35.0 2.0 35.0 2.0 35.0 116-117 24.682287916313886 35.0 2.0 35.0 2.0 35.0 118-119 24.508262975066664 35.0 2.0 35.0 2.0 35.0 120-121 24.283524691993005 35.0 2.0 35.0 2.0 35.0 122-123 24.03730702580375 35.0 2.0 35.0 2.0 35.0 124-125 22.66457701955261 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 6.985189339303588E-5 1101 2 -0.05904964504859578 1101 3 -0.016184794545836212 1101 4 -0.07307726660354774 1101 5 -0.18353769962781996 1101 6 -0.015543546693635335 1101 7 -0.009713363492686256 1101 8 5.233903067747292E-4 1101 9 0.021999986173085517 1101 10-11 -0.04669969859960332 1101 12-13 0.004319189507995702 1101 14-15 -0.05554896995341352 1101 16-17 0.032460747243099775 1101 18-19 -0.010706299192754898 1101 20-21 0.06639251351182907 1101 22-23 0.12381711040329435 1101 24-25 0.1149330126444994 1101 26-27 0.25018801287161807 1101 28-29 0.15399226744967365 1101 30-31 0.21476751321581844 1101 32-33 0.09728114741270133 1101 34-35 -0.06931549377680568 1101 36-37 0.14914489359839678 1101 38-39 0.3738678138019438 1101 40-41 0.28052459894081494 1101 42-43 0.3029325782029204 1101 44-45 0.27853196773062905 1101 46-47 0.09540777105907239 1101 48-49 -0.6656723143270824 1101 50-51 -0.7618383292273165 1101 52-53 -0.8990559524117465 1101 54-55 -1.212085115647561 1101 56-57 -1.3125150838832695 1101 58-59 -0.8168938902977203 1101 60-61 -0.5349687952643052 1101 62-63 -0.7129145677866546 1101 64-65 -0.6997461056728405 1101 66-67 -0.8993633093340208 1101 68-69 -0.9140148242923516 1101 70-71 -1.2148959627641212 1101 72-73 -1.086414044525661 1101 74-75 -1.0449507417455415 1101 76-77 -1.1181244327954794 1101 78-79 -1.1249652753515278 1101 80-81 -0.9264559115821527 1101 82-83 -0.9954961868213985 1101 84-85 -1.3122810092033923 1101 86-87 -1.1624297641869532 1101 88-89 -1.0501452392188142 1101 90-91 -0.982682335177266 1101 92-93 -1.1102739709392466 1101 94-95 -1.0630830922415413 1101 96-97 -1.155136498579676 1101 98-99 -1.1486294586043115 1101 100-101 -1.0939951775575345 1101 102-103 -1.1889063046922566 1101 104-105 -1.1397499254110635 1101 106-107 -1.178325637679091 1101 108-109 -1.1032651715092783 1101 110-111 -1.1307536046557374 1101 112-113 -1.2692002143543348 1101 114-115 -1.14700190097264 1101 116-117 -1.00340165926767 1101 118-119 -1.1527089559574542 1101 120-121 -1.4410404404078996 1101 122-123 -1.2976686913510314 1101 124-125 -1.1388359967799886 1102 1 0.09055367974691109 1102 2 0.11506823787041043 1102 3 0.1572314043314691 1102 4 -0.17762818881686115 1102 5 -0.29584055982562774 1102 6 -0.11665287575003447 1102 7 -0.11329529345793077 1102 8 -0.08902485882447309 1102 9 0.08698929384483023 1102 10-11 0.08849142676793775 1102 12-13 -0.023581104531032793 1102 14-15 0.07620056225722038 1102 16-17 0.19852597044545206 1102 18-19 0.25200754737233666 1102 20-21 0.17715835152304749 1102 22-23 0.26290091152598905 1102 24-25 0.2954161722274975 1102 26-27 0.47098459132657666 1102 28-29 0.31385928161701315 1102 30-31 0.40697882035294697 1102 32-33 0.40894580926782 1102 34-35 0.13325400635685014 1102 36-37 0.44846325767218076 1102 38-39 0.635963510139824 1102 40-41 0.3420890202185518 1102 42-43 0.07288045310268387 1102 44-45 0.08444950314896715 1102 46-47 0.222730011101838 1102 48-49 -0.11069035763101098 1102 50-51 0.10676298058289291 1102 52-53 0.14322886448213623 1102 54-55 0.1361172366646528 1102 56-57 0.4547095886641799 1102 58-59 0.4816726694456648 1102 60-61 0.5722886225384194 1102 62-63 0.45244897137399676 1102 64-65 0.5134488956637 1102 66-67 0.37330776791804965 1102 68-69 0.6579412035077041 1102 70-71 0.853651885404819 1102 72-73 0.7309476234775509 1102 74-75 0.5540535351857585 1102 76-77 0.7682695795007 1102 78-79 0.6689400975434197 1102 80-81 0.6643787582922123 1102 82-83 0.565182776022759 1102 84-85 0.6301307790885105 1102 86-87 0.5276764908250726 1102 88-89 0.5776782037108816 1102 90-91 0.5986461866083488 1102 92-93 0.5718631581706148 1102 94-95 0.5615660657376083 1102 96-97 0.7189613362238525 1102 98-99 0.5614413780703771 1102 100-101 0.4112841595181145 1102 102-103 0.42586915641440015 1102 104-105 0.5027086418169517 1102 106-107 0.35284918188787273 1102 108-109 0.35301322998764917 1102 110-111 0.17503027665941673 1102 112-113 0.11739427909661515 1102 114-115 0.39443087101345853 1102 116-117 0.4997893324457756 1102 118-119 0.5392851632620079 1102 120-121 0.6302473168762504 1102 122-123 0.9210896620304148 1102 124-125 0.6203193092879076 1103 1 0.1156031083853577 1103 2 0.14678634383200517 1103 3 -0.13378598784707663 1103 4 -0.014488548724365558 1103 5 -0.1310002394610308 1103 6 -0.11088899384259321 1103 7 -0.052682378341145863 1103 8 0.050933587608291475 1103 9 0.02519511804643315 1103 10-11 -0.010290450514993665 1103 12-13 -0.1281813190010581 1103 14-15 -0.14108019240918424 1103 16-17 -0.05995229770910271 1103 18-19 0.018503308238258853 1103 20-21 0.0958775633457094 1103 22-23 0.13992413460832154 1103 24-25 0.44415448528670964 1103 26-27 0.23354281658712495 1103 28-29 0.271970604964082 1103 30-31 0.21011041781952144 1103 32-33 0.19497844892467242 1103 34-35 0.3938811636236963 1103 36-37 0.31156268463508496 1103 38-39 0.30702429898737904 1103 40-41 0.05062341919982316 1103 42-43 0.24940352538578026 1103 44-45 -0.021729914430871133 1103 46-47 -0.5403853335581914 1103 48-49 0.16768503193367934 1103 50-51 0.11514909364881021 1103 52-53 -0.056282713548093 1103 54-55 -0.3109731758550325 1103 56-57 0.01219760850265672 1103 58-59 -0.3047956124483733 1103 60-61 -0.3274801519664585 1103 62-63 -0.20055779783615435 1103 64-65 -0.2596527095103909 1103 66-67 -0.5876938741180737 1103 68-69 -0.6472806389238492 1103 70-71 -0.7927783615708215 1103 72-73 -1.1253658378675802 1103 74-75 -0.838284188080884 1103 76-77 -0.9810727682758476 1103 78-79 -0.7964514778059346 1103 80-81 -0.8707784080225878 1103 82-83 -0.6960182561142325 1103 84-85 -0.6471638826185391 1103 86-87 -0.9726753740588094 1103 88-89 -1.1405115352388826 1103 90-91 -0.988733592889659 1103 92-93 -1.2293583156405816 1103 94-95 -1.0294664063645342 1103 96-97 -0.7387269114890245 1103 98-99 -0.576548300559697 1103 100-101 -0.889430461305583 1103 102-103 -0.8772750076551716 1103 104-105 -0.9433764424630695 1103 106-107 -0.8567868892893671 1103 108-109 -1.0805136290318202 1103 110-111 -0.8242031605578362 1103 112-113 -0.7346046485532618 1103 114-115 -0.7220154612140135 1103 116-117 -0.6412372752416324 1103 118-119 -0.7141622748265277 1103 120-121 -0.7619647403604404 1103 122-123 -0.6261146876897676 1103 124-125 -0.5555167886980819 1104 1 0.14902237928144046 1104 2 0.13534941959360225 1104 3 0.32102450777974667 1104 4 0.12851316199533613 1104 5 -0.05788102719525057 1104 6 0.07361975652887054 1104 7 -0.009633281488710566 1104 8 0.10101028889092589 1104 9 0.17124881697582595 1104 10-11 0.32443234060854564 1104 12-13 0.25089167973798965 1104 14-15 0.017197522454807768 1104 16-17 0.17917097851992736 1104 18-19 0.18114215362293606 1104 20-21 0.1554997593315477 1104 22-23 0.15420422681049928 1104 24-25 0.3393508164756227 1104 26-27 0.3451652695824876 1104 28-29 0.33480542011800196 1104 30-31 0.17303277684577978 1104 32-33 0.1972995662736139 1104 34-35 0.4743412106885714 1104 36-37 0.6712475266946925 1104 38-39 0.7557388497427482 1104 40-41 0.781257349277162 1104 42-43 0.7639486586693209 1104 44-45 0.7215255932031184 1104 46-47 0.7853903131822051 1104 48-49 -0.008619492237261994 1104 50-51 -0.2613618413036818 1104 52-53 -0.25953308099141026 1104 54-55 -0.4270892799117689 1104 56-57 -0.40797683671817 1104 58-59 -0.34263625047454127 1104 60-61 -0.21734755111029358 1104 62-63 0.12722573588430208 1104 64-65 0.12921074614342132 1104 66-67 -0.10363248096577138 1104 68-69 -0.016788064089904964 1104 70-71 0.05386324666633513 1104 72-73 -0.17085250951380715 1104 74-75 -0.05830041180768575 1104 76-77 -0.17229990848258225 1104 78-79 -0.21291133211365576 1104 80-81 -0.135409880446268 1104 82-83 -0.06491126786438883 1104 84-85 -0.16370887133421164 1104 86-87 -0.08014150100545692 1104 88-89 -0.1371503324342669 1104 90-91 -0.4250072759165846 1104 92-93 -0.4241233527061752 1104 94-95 -0.3608239789094867 1104 96-97 -0.2604843748049319 1104 98-99 -0.6162683207992004 1104 100-101 -0.3506019428852305 1104 102-103 -0.14317948042420525 1104 104-105 -0.26444961789616883 1104 106-107 -0.3641879606978584 1104 108-109 -0.5296337729571583 1104 110-111 -0.39050358863934065 1104 112-113 -0.36412942186282393 1104 114-115 -0.16252980154282426 1104 116-117 -0.21614350691966422 1104 118-119 -0.18854565273495183 1104 120-121 -0.35298627294041296 1104 122-123 -0.48642197471319193 1104 124-125 -0.3715943413248013 1105 1 0.09841985838943401 1105 2 0.06546315214512077 1105 3 0.17018651895293146 1105 4 -0.08241532039073718 1105 5 -0.2178530172840496 1105 6 0.056659909198273795 1105 7 0.13089336640207705 1105 8 0.12044024102783624 1105 9 0.037801597190366465 1105 10-11 0.17861535921512228 1105 12-13 0.21456344051487974 1105 14-15 0.23557094689730462 1105 16-17 0.25946477478630214 1105 18-19 0.22179500001472974 1105 20-21 0.04380254339359624 1105 22-23 -0.012624035497701414 1105 24-25 0.08582264496877912 1105 26-27 0.2011104501848635 1105 28-29 0.15809289106926627 1105 30-31 -0.16969234127294186 1105 32-33 0.20861510869632127 1105 34-35 0.25674498433154724 1105 36-37 0.45101575237455194 1105 38-39 0.5539866912866813 1105 40-41 0.3433331607181316 1105 42-43 0.7094870799568511 1105 44-45 0.7250614597405018 1105 46-47 0.9433923754503866 1105 48-49 1.580746745046703 1105 50-51 1.5865035572219455 1105 52-53 1.2489968603724542 1105 54-55 1.139480429369975 1105 56-57 1.073601260146873 1105 58-59 1.0571398241364776 1105 60-61 1.248940520053349 1105 62-63 1.351785964888414 1105 64-65 1.2630234798799727 1105 66-67 1.0904785121548706 1105 68-69 1.2358243987815776 1105 70-71 1.2599400122910964 1105 72-73 1.2628778873958701 1105 74-75 1.248588949043075 1105 76-77 1.1287412291507373 1105 78-79 1.1459194849446916 1105 80-81 1.1577246132976704 1105 82-83 0.9134618487768869 1105 84-85 0.9687456895103956 1105 86-87 0.9585183324740818 1105 88-89 1.0977289325364161 1105 90-91 1.1655247584955895 1105 92-93 1.2230864292166572 1105 94-95 1.3086282888160135 1105 96-97 1.30129133044462 1105 98-99 1.2518350080710192 1105 100-101 1.2640501647836366 1105 102-103 1.3908325546035734 1105 104-105 1.5390616245434643 1105 106-107 1.6316490277749836 1105 108-109 1.556642909561269 1105 110-111 1.705684293226554 1105 112-113 1.9413363583363257 1105 114-115 1.5826492618180552 1105 116-117 1.4138794242713715 1105 118-119 1.381086678705401 1105 120-121 1.42091064443035 1105 122-123 1.649810846131107 1105 124-125 1.1835136199538034 1106 1 0.29648719947353186 1106 2 0.1635304932292172 1106 3 0.21291639967164144 1106 4 0.220181690027502 1106 5 0.14746481451422966 1106 6 0.22307996053452683 1106 7 0.2178479187197624 1106 8 0.08550139156384517 1106 9 0.4666102941554868 1106 10-11 0.14736064080957334 1106 12-13 0.12091252703156385 1106 14-15 0.20057925129108156 1106 16-17 0.17472976044233945 1106 18-19 0.19472267629436857 1106 20-21 0.41788528535343517 1106 22-23 0.18964834861825608 1106 24-25 0.28521113960866984 1106 26-27 0.3243552033178858 1106 28-29 0.2806657432510562 1106 30-31 0.44188096387578213 1106 32-33 0.38575537745670374 1106 34-35 0.5420688556889459 1106 36-37 0.49389662207105545 1106 38-39 0.5309231249270212 1106 40-41 0.7460887095630682 1106 42-43 0.7025868836711808 1106 44-45 0.5588980896665205 1106 46-47 0.3237940061313509 1106 48-49 -0.21940424393110547 1106 50-51 -0.15365951087408192 1106 52-53 0.28107446870705033 1106 54-55 0.19435737335306413 1106 56-57 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-0.3049200704395503 2216 26-27 -0.4917544748249938 2216 28-29 -0.6912082053863742 2216 30-31 -1.111085280376777 2216 32-33 -1.0301131882043961 2216 34-35 -1.1107067414849965 2216 36-37 -0.8423151083315403 2216 38-39 -0.9726655259645298 2216 40-41 -1.2637856876557265 2216 42-43 -1.6322454590449738 2216 44-45 -1.466082284898917 2216 46-47 -1.4489266398258138 2216 48-49 -1.6524702975365777 2216 50-51 -1.6712214711746292 2216 52-53 -0.9962104926028097 2216 54-55 -0.9604929126175534 2216 56-57 -1.2200889042055678 2216 58-59 -1.0838231096357518 2216 60-61 -1.198386724435398 2216 62-63 -1.2541912251252647 2216 64-65 -1.39763161180041 2216 66-67 -1.3548841527191442 2216 68-69 -1.0905623973889433 2216 70-71 -1.206627362937816 2216 72-73 -1.0350775589519827 2216 74-75 -1.0363504329546522 2216 76-77 -1.069791421119323 2216 78-79 -1.147521484943347 2216 80-81 -1.0933417072011835 2216 82-83 -1.2371733073463425 2216 84-85 -1.3706919423308612 2216 86-87 -1.3000527980574574 2216 88-89 -1.2640492727998236 2216 90-91 -1.2411258360939925 2216 92-93 -1.11289145622343 2216 94-95 -1.327008837682051 2216 96-97 -1.4691020490959374 2216 98-99 -1.3859521450850565 2216 100-101 -1.4536762544657655 2216 102-103 -1.6323830971268798 2216 104-105 -1.5705433732543526 2216 106-107 -1.5584399868057908 2216 108-109 -1.5066293035173821 2216 110-111 -1.4528671335566372 2216 112-113 -1.4933631015491606 2216 114-115 -1.7441142236591247 2216 116-117 -1.6272376623555793 2216 118-119 -1.489158343200529 2216 120-121 -1.4708520297160135 2216 122-123 -1.8326006608579313 2216 124-125 -1.8947102609891964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 12.0 5 69.0 6 164.0 7 352.0 8 608.0 9 1267.0 10 2555.0 11 3460.0 12 3951.0 13 6214.0 14 11818.0 15 12266.0 16 3525.0 17 2246.0 18 2069.0 19 2264.0 20 2175.0 21 2693.0 22 2575.0 23 1508.0 24 1593.0 25 1696.0 26 1896.0 27 2166.0 28 2434.0 29 2800.0 30 3294.0 31 4030.0 32 5091.0 33 6391.0 34 9199.0 35 19732.0 36 23593.0 37 62566.0 38 44333.0 39 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.84369867621743 20.63873993311831 16.6064467617785 15.911114628885766 2 15.645351459568104 23.757843843226006 42.2760399768938 18.32076472031209 3 14.938358549091598 35.306149901880104 35.026824713553204 14.72866683547509 4 9.133130612438828 21.35854674458751 39.80060499516954 29.70771764780412 5 7.569654910463469 40.97077698559028 34.484818750840766 16.97474935310549 6 20.86316131049193 44.2668946058354 17.389859081357663 17.480085002315008 7 18.754599333739524 41.609232692657685 23.343250749740854 16.292917223861934 8 25.316315468795747 32.877422315769486 20.854269368635116 20.951992846799655 9 28.53169484953749 13.055381912848066 22.04910703688171 36.36381620073273 10-11 21.96186806143523 29.824651637560635 26.713721642387462 21.49975865861668 12-13 27.21341699834622 29.770844378328338 28.66126747746821 14.354471145857236 14-15 22.928618340362565 26.72460178671752 33.79016751465852 16.556612358261393 16-17 14.625683470884734 30.586063919858837 33.96879970247751 20.819452906778924 18-19 14.535477183348236 22.197274820576528 46.53160780838285 16.73564018769238 20-21 16.4131922739106 21.334182643993767 48.1343519786037 14.118273103491932 22-23 15.892330091709725 22.275414038946963 47.74622755722956 14.086028312113754 24-25 14.995697371934996 22.426088762722426 48.86321599192887 13.714997873413715 26-27 14.323413279315064 22.486291018144126 48.87519089707779 14.315104805463019 28-29 14.594683346983023 20.93565655007062 49.62136947929402 14.84829062365234 30-31 14.69974954399598 21.441684900233046 48.85395605743474 15.004609498336228 32-33 14.505210600030068 21.984419633319355 48.788941025661316 14.721428740989262 34-35 14.975430655894664 21.80064410454434 48.42040086408128 14.803524375479714 36-37 14.926964558424396 21.555148484296986 46.98916733266338 16.52871962461524 38-39 14.830823646707866 21.7029206263798 47.45088109386993 16.015374633042402 40-41 14.750904041083427 21.60717535627527 47.18975762994849 16.452162972692815 42-43 15.025677140618942 21.565435166209042 46.105106150644495 17.303781542527517 44-45 15.14772648591974 22.195867417086223 44.644160674190644 18.012245422803392 46-47 16.634586281728424 22.35504974273048 41.69230130108546 19.31806267445564 48-49 16.753048418620477 23.765795991359187 38.51551310760661 20.96564248241373 50-51 17.04549950544016 24.293372898120673 37.24035608308605 21.420771513353117 52-53 16.717440673540278 24.99347191340196 37.07577328153066 21.2133141315271 54-55 16.950379723797674 25.073244457456887 36.26021020813098 21.716165610614464 56-57 17.83694389988665 25.205981321722547 34.95362082028537 22.003453958105425 58-59 16.930728129123384 24.801929175214294 36.439888348391 21.82745434727132 60-61 18.30637360115686 25.905279254293212 35.11543855970343 20.6729085848465 62-63 19.31270784247395 25.88730560979325 35.60854693753202 19.191439610200774 64-65 19.155291790306627 25.40316518298714 36.34757665677547 19.09396636993076 66-67 19.167055714251802 25.819571599262524 35.528814578602116 19.484558107883554 68-69 19.44949990504526 25.317702728366147 35.91742102930936 19.31537633727923 70-71 19.011078140454995 25.237784371909 36.04826904055391 19.702868447082096 72-73 18.612214537800497 25.127993099846485 35.55260575751341 20.707186604839602 74-75 17.473907435688457 25.716111317723957 35.75927581759339 21.0507054289942 76-77 16.93484892245728 25.61315286586403 36.73378146871823 20.718216742960465 78-79 16.59235139583465 24.875371906058113 36.62245996075204 21.909816737355197 80-81 16.486385972131004 25.771501143404258 35.89023319116612 21.851879693298624 82-83 16.813121162245995 24.441349623346206 35.81910331075521 22.926425903652593 84-85 16.602704606059646 23.917722370368026 35.62752716079666 23.852045862775665 86-87 16.384997794258744 24.737338748444607 35.1893871204493 23.688276336847352 88-89 16.672205802640587 24.082778430604517 35.981435981435986 23.263579785318917 90-91 16.744478646546334 24.181787248858537 35.5477435844682 23.52599052012693 92-93 16.65206029037344 24.3412788063788 35.11860839151155 23.88805251173621 94-95 16.30988907946041 23.602513961507192 35.778056051766875 24.30954090726552 96-97 16.762547231399235 23.878612831114367 35.195552829927394 24.163287107559004 98-99 16.51137118982053 23.790080081030606 35.04455100813471 24.653997721014147 100-101 16.634124049686346 23.957894403659015 35.55355090397258 23.854430642682065 102-103 17.33682557141218 23.769836716768676 34.678794544828705 24.21454316699044 104-105 16.437193623814554 24.11524294469304 34.89098052248638 24.556582909006025 106-107 16.72297965610831 23.790919233721326 34.75810471842187 24.7279963917485 108-109 16.632540619887276 24.254815729239 33.96303308347132 25.1496105674024 110-111 16.88512850541242 24.56459296068874 33.655322213078435 24.89495632082041 112-113 16.55047577597974 24.376941185780137 33.90670438584344 25.165878652396685 114-115 16.256913837169737 24.894561510401417 33.457432918964685 25.39109173346416 116-117 15.804497653845239 24.937488625303654 33.67384888072988 25.584164840121225 118-119 15.916985820092421 24.566966829144775 33.67114800278534 25.844899347977464 120-121 15.806507130465059 25.126655021592182 33.15146971258559 25.915368135357177 122-123 16.12697106435002 25.357022043477656 32.53406026892133 25.98194662325099 124-125 15.509021498417749 25.477099425481846 32.19458809047424 26.81929098562616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1902.0 1 1321.0 2 1186.5 3 1382.5 4 687.5 5 431.5 6 1082.5 7 2620.0 8 4284.0 9 4483.0 10 2800.5 11 1238.5 12 923.5 13 869.5 14 851.5 15 907.0 16 994.5 17 1028.5 18 1051.0 19 1107.5 20 1165.0 21 1243.5 22 1359.5 23 1526.5 24 1691.5 25 1773.0 26 1919.5 27 2158.5 28 2421.5 29 2922.0 30 3675.0 31 4290.5 32 5335.0 33 6655.5 34 7860.5 35 8785.0 36 10405.0 37 12103.5 38 12039.0 39 11636.5 40 11262.5 41 10195.0 42 8992.0 43 9045.5 44 9321.0 45 8960.0 46 7977.0 47 7059.5 48 6413.0 49 5414.0 50 4653.5 51 3655.0 52 2912.0 53 2585.5 54 2334.5 55 2474.5 56 2728.5 57 2500.5 58 2082.5 59 1959.5 60 1832.5 61 1803.0 62 1603.0 63 901.0 64 422.0 65 523.0 66 567.5 67 363.0 68 202.5 69 116.5 70 115.0 71 100.0 72 90.5 73 157.0 74 128.0 75 44.5 76 29.0 77 29.5 78 25.0 79 5.5 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02729927122815069 2 0.0031651328960174713 3 7.912832240043678E-4 4 0.07517190628041494 5 0.0031651328960174713 6 0.021760288660120114 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.934624180032759E-4 26-27 0.0 28-29 3.956416120021839E-4 30-31 0.00593462418003276 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.002571670478014196 46-47 0.0013847456420076438 48-49 0.0 50-51 0.0015825664480087357 52-53 0.0 54-55 0.0017803872540098277 56-57 1.9782080600109196E-4 58-59 0.0013847456420076438 60-61 1.9782080600109196E-4 62-63 0.0033629537020185636 64-65 0.0015825664480087357 66-67 0.0 68-69 7.912832240043678E-4 70-71 0.0015825664480087357 72-73 0.0023738496720131035 74-75 0.0 76-77 0.0011869248360065518 78-79 7.912832240043678E-4 80-81 0.0 82-83 7.912832240043678E-4 84-85 0.0 86-87 0.0017803872540098277 88-89 0.0027694912840152876 90-91 0.0031651328960174713 92-93 0.0041542369260229315 94-95 0.0021760288660120117 96-97 0.0035607745080196555 98-99 0.003956416120021839 100-101 0.0033629537020185636 102-103 0.0011869248360065518 104-105 3.956416120021839E-4 106-107 0.0 108-109 0.005736803374031667 110-111 7.912832240043678E-4 112-113 0.0035607745080196555 114-115 0.0 116-117 0.0 118-119 7.912832240043678E-4 120-121 1.9782080600109196E-4 122-123 0.0013847456420076438 124-125 0.042531473290234774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 252754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.679269170814308 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38820218518927 20.18682196918743 2 10.691424787551043 6.1324449860338515 3 4.93598940514292 4.246817063231442 4 3.000496633925615 3.4420820244190007 5 1.918938307030129 2.751687411475189 6 1.4333406908729722 2.4664298092216144 7 1.042931243792076 2.0937354107155572 8 0.7835779715263216 1.7977954849379238 9 0.6470036419821212 1.6700032442612183 >10 4.5635139609314646 24.821367812181013 >50 0.36557775080013244 7.12669235699534 >100 0.2041717249751683 9.664337656377347 >500 0.011036309458117205 2.006694256075077 >1k 0.011036309458117205 5.731264391463636 >5k 0.0027590773645293012 5.861826123424358 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8198 3.243469935193904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6618 2.6183561882304534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 1.802147542669948 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1796 0.7105723351559224 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1795 0.7101766935439202 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.621552972455431 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1457 0.5764498286871821 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.4692309518345901 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1098 0.43441448997839793 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1028 0.4067195771382451 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 981 0.38812442137414244 RNA PCR Primer, Index 26 (100% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 650 0.2571670478014196 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 631 0.24964985717337806 RNA PCR Primer, Index 26 (100% over 24bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.23857189203731693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 582 0.23026341818527105 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.22432879400523828 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 535 0.2116682624211684 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 523 0.20692056307714218 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 438 0.17329102605695657 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 424 0.16775204348892597 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 423 0.1673564018769238 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 409 0.16181741930889323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 386 0.152717662232843 No Hit CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT 366 0.1448048299927993 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 329 0.1301660903487185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 328 0.12977044873671634 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 328 0.12977044873671634 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 304 0.12027505004866391 RNA PCR Primer, Index 26 (100% over 29bp) CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 289 0.11434042586863115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 283 0.11196657619661807 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 280 0.11077965136061149 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 279 0.11038400974860932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 278 0.10998836813660713 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 269 0.10642759362858747 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 261 0.10326246073257002 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 254 0.10049296944855474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9782080600109196E-4 0.0 16-17 0.0 0.0 0.0 5.934624180032759E-4 0.0 18-19 0.0 0.0 0.0 0.0013847456420076438 0.0 20-21 0.0 0.0 0.0 0.0029673120900163795 0.0 22-23 0.0 0.0 0.0 0.0077150114340425865 0.0 24-25 0.0 0.0 0.0 0.017210410122095003 0.0 26-27 0.0 0.0 0.0 0.027694912840152873 0.0 28-29 0.0 0.0 0.0 0.05380725923229701 0.0 30-31 0.0 0.0 0.0 0.1380789225887622 0.0 32-33 0.0 0.0 0.0 0.24529779944135405 0.0 34-35 0.0 0.0 0.0 0.3792224851040933 0.0 36-37 0.0 0.0 0.0 0.5590415977590859 0.0 38-39 0.0 0.0 0.0 0.8559706275667249 0.0 40-41 0.0 0.0 0.0 1.213235003204697 0.0 42-43 0.0 0.0 0.0 1.4866233570982061 0.0 44-45 0.0 0.0 0.0 1.7799916123978257 0.0 46-47 0.0 0.0 0.0 2.1170782658236864 0.0 48-49 0.0 0.0 0.0 2.51093949057186 0.0 50-51 0.0 0.0 0.0 2.944364876520253 0.0 52-53 0.0 0.0 0.0 3.324180824042349 0.0 54-55 0.0 0.0 0.0 3.661069656662209 0.0 56-57 0.0 0.0 0.0 4.04345727466232 0.0 58-59 0.0 0.0 0.0 4.493301787508803 0.0 60-61 0.0 0.0 0.0 5.045617477863852 0.0 62-63 0.0 0.0 0.0 5.5664796600647275 0.0 64-65 0.0 0.0 0.0 6.037095357541325 0.0 66-67 0.0 0.0 0.0 6.505930667763913 0.0 68-69 0.0 0.0 0.0 7.022638613038765 0.0 70-71 0.0 0.0 0.0 7.6331136203581345 0.0 72-73 0.0 0.0 0.0 8.28058111839971 0.0 74-75 0.0 0.0 0.0 8.859800438370907 0.0 76-77 0.0 0.0 0.0 9.397873030693876 0.0 78-79 0.0 0.0 0.0 10.036636413271403 0.0 80-81 0.0 0.0 0.0 10.771936349177462 0.0 82-83 0.0 0.0 0.0 11.519105533443586 0.0 84-85 0.0 0.0 0.0 12.250251232423622 0.0 86-87 0.0 0.0 0.0 12.960032284355538 0.0 88-89 0.0 0.0 0.0 13.737468051939832 0.0 90-91 0.0 0.0 0.0 14.585723668072514 0.0 92-93 0.0 0.0 0.0 15.546737143625819 0.0 94-95 0.0 0.0 0.0 16.475703648606945 0.0 96-97 0.0 0.0 0.0 17.357193160147812 0.0 98-99 0.0 0.0 0.0 18.258069110676786 0.0 100-101 0.0 0.0 0.0 19.233919146680172 0.0 102-103 0.0 0.0 0.0 20.315998955506146 0.0 104-105 0.0 0.0 0.0 21.386802978390055 0.0 106-107 0.0 0.0 0.0 22.437429279061853 0.0 108-109 0.0 0.0 0.0 23.433852678889355 0.0 110-111 0.0 0.0 0.0 24.543231758943477 0.0 112-113 0.0 0.0 0.0 25.672986382015715 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2215 0.0 97.10983 1 GTGGTAT 390 0.0 91.66771 1 TCACGCA 20 8.4331346E-4 89.35831 4 CATATGC 20 8.4331346E-4 89.35831 3 ATCACGC 20 8.4331346E-4 89.35831 3 CATGGGT 55 2.0008883E-11 86.65048 4 GTGTATC 25 0.0020435136 71.50082 1 GGTACAC 25 0.0020451206 71.48665 3 CACGCAG 25 0.0020563956 71.38766 5 GTGGTAC 90 2.3646862E-11 59.48971 6 GTATCAA 4585 0.0 58.869263 1 TGGTATC 600 0.0 58.579338 2 GGTACTC 95 4.1836756E-11 56.358673 8 TAGGCAT 55 1.309111E-5 54.081554 5 TGGTACT 110 3.6379788E-12 54.081554 7 GTATGGT 100 6.730261E-11 53.62561 1 GTTAGAG 45 3.14728E-4 52.95307 3 GGGTACA 35 0.007751992 51.06189 2 AGATAGT 95 2.6229827E-9 50.0966 6 ACCACTG 100 4.130925E-9 47.59177 9 >>END_MODULE