##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 123621 Sequences flagged as poor quality 0 Sequence length 125 %GC 26 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.988602260133796 26.0 10.0 33.0 7.0 34.0 2 22.633347085042185 26.0 12.0 34.0 7.0 34.0 3 23.799880279240583 27.0 16.0 34.0 7.0 34.0 4 24.612832771131117 30.0 12.0 37.0 7.0 37.0 5 27.605471562275017 31.0 25.0 37.0 7.0 37.0 6 25.30161542132809 31.0 13.0 37.0 2.0 37.0 7 26.124914051819676 32.0 13.0 37.0 2.0 37.0 8 24.891329143106752 31.0 12.0 37.0 2.0 37.0 9 24.93927407155742 31.0 8.0 39.0 2.0 39.0 10-11 26.278965547924706 31.0 11.5 39.0 2.0 39.0 12-13 26.360246236480855 32.0 11.5 39.0 2.0 39.0 14-15 27.109920644550684 32.0 12.5 41.0 2.0 41.0 16-17 27.545186497439758 32.0 14.5 40.5 2.0 41.0 18-19 27.067270124008058 31.5 13.0 40.0 2.0 41.0 20-21 26.695177194813176 31.0 13.0 40.0 2.0 41.0 22-23 26.71156599606863 30.5 17.5 40.0 2.0 41.0 24-25 26.650601435031266 30.0 19.0 40.0 2.0 41.0 26-27 26.528138423083455 30.0 20.5 40.0 2.0 41.0 28-29 26.329401962449744 30.0 19.0 40.0 2.0 41.0 30-31 26.00319524999798 30.0 10.5 40.0 2.0 41.0 32-33 25.721317575492836 30.0 2.0 39.0 2.0 41.0 34-35 25.355983206736717 30.0 2.0 39.0 2.0 41.0 36-37 24.78770597228626 30.0 2.0 39.0 2.0 41.0 38-39 24.32026516530363 30.0 2.0 39.0 2.0 41.0 40-41 23.990240331335293 29.0 2.0 39.0 2.0 41.0 42-43 23.66260991255531 28.0 2.0 39.0 2.0 41.0 44-45 23.558990786355068 28.0 2.0 39.0 2.0 41.0 46-47 23.291257148866293 27.5 2.0 39.0 2.0 41.0 48-49 23.03055306137307 27.0 2.0 39.0 2.0 41.0 50-51 22.876101147863224 27.0 2.0 38.5 2.0 41.0 52-53 22.700111631518915 27.0 2.0 38.0 2.0 41.0 54-55 22.60936653157635 27.5 2.0 38.0 2.0 41.0 56-57 22.50046513132882 28.0 2.0 37.5 2.0 41.0 58-59 22.294395774180764 28.0 2.0 37.0 2.0 41.0 60-61 22.087189878742286 28.0 2.0 37.0 2.0 41.0 62-63 21.84328714377007 28.0 2.0 36.0 2.0 41.0 64-65 21.622167754669512 28.0 2.0 36.0 2.0 41.0 66-67 21.38936750228521 28.0 2.0 35.0 2.0 40.5 68-69 21.136158096116354 28.0 2.0 35.0 2.0 40.0 70-71 21.004121468035365 28.0 2.0 35.0 2.0 40.0 72-73 20.822154002960662 28.0 2.0 35.0 2.0 39.5 74-75 20.596686647090706 28.0 2.0 35.0 2.0 39.0 76-77 20.374754289319775 27.5 2.0 35.0 2.0 39.0 78-79 20.161436972682637 27.0 2.0 35.0 2.0 37.5 80-81 19.930533647195865 26.0 2.0 35.0 2.0 37.0 82-83 19.7207311055565 26.0 2.0 35.0 2.0 37.0 84-85 19.513367469928248 25.0 2.0 35.0 2.0 36.0 86-87 19.33238689219469 25.0 2.0 35.0 2.0 36.0 88-89 19.176284773622605 25.0 2.0 35.0 2.0 36.0 90-91 19.012534278156625 25.0 2.0 35.0 2.0 35.0 92-93 18.858163257051793 25.0 2.0 35.0 2.0 35.0 94-95 18.751292256170068 25.0 2.0 35.0 2.0 35.0 96-97 18.61222203347328 25.0 2.0 35.0 2.0 35.0 98-99 18.495809773420373 25.0 2.0 35.0 2.0 35.0 100-101 18.426836864286813 25.0 2.0 35.0 2.0 35.0 102-103 17.96714959432459 25.0 2.0 33.5 2.0 35.0 104-105 18.22536624036369 25.0 2.0 35.0 2.0 35.0 106-107 18.217827068216565 25.0 2.0 35.0 2.0 35.0 108-109 18.134196455294813 25.0 2.0 35.0 2.0 35.0 110-111 18.015296753787787 25.0 2.0 35.0 2.0 35.0 112-113 17.880424846911126 25.0 2.0 35.0 2.0 35.0 114-115 17.712807694485566 24.5 2.0 35.0 2.0 35.0 116-117 17.577984323051908 24.0 2.0 35.0 2.0 35.0 118-119 17.41122058549923 24.0 2.0 34.0 2.0 35.0 120-121 17.220249795746675 23.5 2.0 34.0 2.0 35.0 122-123 16.99992315221524 20.0 2.0 34.0 2.0 35.0 124-125 16.19645933943262 9.5 2.0 32.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.28558380164880504 1101 2 0.09862988907296 1101 3 0.13918098200030116 1101 4 0.04450726349716305 1101 5 1.0593029351924983 1101 6 0.8296605580731331 1101 7 -0.2243431510336933 1101 8 -0.31398680407698976 1101 9 0.2769505095056246 1101 10-11 0.434447920386301 1101 12-13 0.4261991611876539 1101 14-15 1.171310971249433 1101 16-17 0.16436407893705862 1101 18-19 -0.014505244807946127 1101 20-21 -0.19634265240150128 1101 22-23 0.021373338216132964 1101 24-25 0.30754324171265424 1101 26-27 0.30805224553535027 1101 28-29 0.31761475073268386 1101 30-31 0.34853671451390866 1101 32-33 0.5370051775918867 1101 34-35 0.3161424420767851 1101 36-37 0.22733647565628345 1101 38-39 0.5762694945478835 1101 40-41 0.7006587209278976 1101 42-43 0.5871720976774775 1101 44-45 0.774350402955335 1101 46-47 0.8069731749016178 1101 48-49 0.847406181711527 1101 50-51 0.8636564829990832 1101 52-53 1.0433101801639992 1101 54-55 0.9953979188474662 1101 56-57 1.0056234669880766 1101 58-59 1.3301528702023617 1101 60-61 1.2178877991863502 1101 62-63 1.2435512181450186 1101 64-65 1.289068467826386 1101 66-67 1.373414030025284 1101 68-69 1.3749326857371607 1101 70-71 1.1981557345506069 1101 72-73 1.438770745164831 1101 74-75 1.4218283190433034 1101 76-77 1.4569858286907902 1101 78-79 1.355200865647884 1101 80-81 1.2510223618569327 1101 82-83 1.6130148107730626 1101 84-85 1.2827696215529159 1101 86-87 1.4368931084503203 1101 88-89 1.3833667975919717 1101 90-91 1.387079032428705 1101 92-93 1.0359762533755053 1101 94-95 1.0265690242436492 1101 96-97 0.6463076550835858 1101 98-99 0.8564938227724639 1101 100-101 0.850277157886083 1101 102-103 0.9470976781105449 1101 104-105 0.795892838830035 1101 106-107 0.5874493823529754 1101 108-109 0.5906346878163333 1101 110-111 0.35946096158481566 1101 112-113 0.29625952117500276 1101 114-115 0.36142636009408946 1101 116-117 0.527679603341209 1101 118-119 0.5484149658862947 1101 120-121 0.607060483205629 1101 122-123 0.8341768434613321 1101 124-125 0.8352709322693634 1102 1 -0.4879229829353555 1102 2 0.725895970944304 1102 3 -0.3399856846663667 1102 4 1.445713403848039 1102 5 0.7702312977655978 1102 6 1.1021020785409696 1102 7 1.9573088957499323 1102 8 1.0835643655136522 1102 9 1.277169807751239 1102 10-11 1.3697366630763597 1102 12-13 1.768340974053153 1102 14-15 1.1613511759277948 1102 16-17 1.65519010232887 1102 18-19 1.3591241411569648 1102 20-21 0.690371528885052 1102 22-23 0.4223601803213981 1102 24-25 0.3422837387886766 1102 26-27 -0.2499886901371653 1102 28-29 -0.8988691673959721 1102 30-31 -0.8414852153106516 1102 32-33 -0.7473149978467077 1102 34-35 -0.7411309497360854 1102 36-37 -0.58112478165366 1102 38-39 -0.47349293235270196 1102 40-41 -0.3510591153294129 1102 42-43 -0.3881020251295446 1102 44-45 -0.03575559119671112 1102 46-47 -0.0878915911802558 1102 48-49 -0.02985990015982054 1102 50-51 0.23500151223884913 1102 52-53 0.23981968308797263 1102 54-55 0.20055142761939848 1102 56-57 0.24897142020444818 1102 58-59 0.0966185134772104 1102 60-61 0.3534141149758234 1102 62-63 0.43106949299881947 1102 64-65 0.19223001753398705 1102 66-67 0.2434798194989689 1102 68-69 0.4068771301816021 1102 70-71 0.6626842433225377 1102 72-73 0.4975792246969917 1102 74-75 0.6407976172889214 1102 76-77 0.5106590743048258 1102 78-79 0.5710451638935012 1102 80-81 0.7897467770616124 1102 82-83 0.6763737289017193 1102 84-85 0.6104925747692889 1102 86-87 0.46585875172517177 1102 88-89 0.9191489613346597 1102 90-91 1.127009587984265 1102 92-93 1.185153884954449 1102 94-95 1.249522240617921 1102 96-97 1.1207228597619512 1102 98-99 0.982572039146735 1102 100-101 0.7445388537925162 1102 102-103 0.7650618593970968 1102 104-105 0.9875412306429041 1102 106-107 0.6132900256278262 1102 108-109 0.954432202436216 1102 110-111 0.8496290902397874 1102 112-113 0.8458757492451774 1102 114-115 1.066799167111629 1102 116-117 0.9597154220546606 1102 118-119 0.9272892348921431 1102 120-121 0.7953463019190785 1102 122-123 0.5463661709467118 1102 124-125 0.6035640609243345 1103 1 -1.3332582202534589 1103 2 -1.5371259248805274 1103 3 -0.6791329714880696 1103 4 -1.1123241318516754 1103 5 -1.1786331113191295 1103 6 -1.6729557209966366 1103 7 -1.7694012905685774 1103 8 -1.4679112226816429 1103 9 -0.8570611184013508 1103 10-11 -0.752543358683468 1103 12-13 -0.9003996760216495 1103 14-15 -0.3275989124714975 1103 16-17 -1.1645603396675916 1103 18-19 -0.6827465238777144 1103 20-21 0.018264905738035964 1103 22-23 -0.1642371269001437 1103 24-25 -0.168256176892001 1103 26-27 -0.17218031260418698 1103 28-29 -0.3828212957789461 1103 30-31 -0.5739923552535338 1103 32-33 -0.7605238921755557 1103 34-35 -0.6476656974581019 1103 36-37 -0.9301489894599975 1103 38-39 -1.227436900800953 1103 40-41 -0.7957075581418707 1103 42-43 -0.2697319720899678 1103 44-45 -0.793745527277224 1103 46-47 -0.7883029878890788 1103 48-49 -0.688349632241966 1103 50-51 -1.1973900286288242 1103 52-53 -0.8717334244871644 1103 54-55 -0.8550381276641623 1103 56-57 -1.1580393237096018 1103 58-59 -1.0177395716581046 1103 60-61 -0.9937401077903907 1103 62-63 -0.848527270227077 1103 64-65 -0.7826611833364012 1103 66-67 -0.782835969974716 1103 68-69 -0.8486138258907481 1103 70-71 -0.4481378701005596 1103 72-73 -0.5500373943700545 1103 74-75 -0.5254100530497183 1103 76-77 -0.7551507992161852 1103 78-79 -0.5137671576079299 1103 80-81 -0.504282870701207 1103 82-83 -0.4385113520176347 1103 84-85 -0.5174484017029002 1103 86-87 -0.6252435194566566 1103 88-89 -0.36852273729175167 1103 90-91 -0.30354596757129215 1103 92-93 -0.10799176988031078 1103 94-95 -0.3193612083144899 1103 96-97 -0.5397388565443215 1103 98-99 -0.3479393167624174 1103 100-101 -0.3748681909511298 1103 102-103 -0.30530057770340946 1103 104-105 -0.4053426262862416 1103 106-107 -0.4574634083447009 1103 108-109 -0.44054263776506275 1103 110-111 -0.1982425267872756 1103 112-113 0.005198474663373531 1103 114-115 0.013606592652227079 1103 116-117 0.05042669636446462 1103 118-119 0.3882405472816437 1103 120-121 0.3133831576242301 1103 122-123 0.1803250992752865 1103 124-125 0.36673895552517877 1104 1 1.0968723387020702 1104 2 0.9715100060320232 1104 3 1.250657590187437 1104 4 1.369690012035175 1104 5 0.6006406544907463 1104 6 1.324324300763191 1104 7 1.2614024630013958 1104 8 1.7209912660984479 1104 9 1.663134720031941 1104 10-11 1.1826021601523848 1104 12-13 1.6279900968601702 1104 14-15 1.521000298734812 1104 16-17 1.979751505837644 1104 18-19 1.7100013341394238 1104 20-21 1.763470944089729 1104 22-23 1.6708981920173045 1104 24-25 1.631757422999204 1104 26-27 2.273403122728329 1104 28-29 2.2151659203233294 1104 30-31 2.1205030887829146 1104 32-33 2.182509563556799 1104 34-35 2.130214079503684 1104 36-37 2.1118576744866893 1104 38-39 2.3569164243724465 1104 40-41 1.8536192472436852 1104 42-43 1.816576337443557 1104 44-45 1.8063496719611827 1104 46-47 1.9442721515098036 1104 48-49 1.8180933162144477 1104 50-51 1.8022529742271516 1104 52-53 1.6316325485850491 1104 54-55 1.3730660475024372 1104 56-57 1.8659304845319333 1104 58-59 2.011823191839788 1104 60-61 1.8651100214085723 1104 62-63 1.6796075046947223 1104 64-65 1.6279025321538718 1104 66-67 1.5095616908439986 1104 68-69 1.5209122179009036 1104 70-71 1.504789506480428 1104 72-73 1.7958248387320808 1104 74-75 1.4945987868795605 1104 76-77 1.6919456240124262 1104 78-79 1.6470685557063653 1104 80-81 1.7371151981142425 1104 82-83 1.3050286996619533 1104 84-85 1.072480878862855 1104 86-87 1.1471453014327757 1104 88-89 1.1852308326796894 1104 90-91 0.9223312254111633 1104 92-93 0.9541597329076659 1104 94-95 0.9688204862319552 1104 96-97 0.8049333860777388 1104 98-99 0.7808176531818205 1104 100-101 0.42290142689192933 1104 102-103 0.6656466547187314 1104 104-105 0.7740909382452408 1104 106-107 0.37059996714829424 1104 108-109 0.6444906819683816 1104 110-111 0.4724361077836505 1104 112-113 0.4365190240989776 1104 114-115 0.4820038454742068 1104 116-117 0.5591306267330225 1104 118-119 0.40389742202664536 1104 120-121 0.46201296858574636 1104 122-123 0.3826234808882347 1104 124-125 0.05678043519334253 1105 1 0.6115260249407903 1105 2 0.39364433994001047 1105 3 0.9047156640812268 1105 4 0.3245289397977409 1105 5 0.6522581375046386 1105 6 0.32973281240839114 1105 7 0.6336770801801812 1105 8 -0.06503449193826327 1105 9 0.5362713187541814 1105 10-11 1.213295463738902 1105 12-13 1.2189376004940158 1105 14-15 0.7151151620008775 1105 16-17 1.1899059864515067 1105 18-19 1.2007223563481233 1105 20-21 1.741211538349944 1105 22-23 1.5016117775224949 1105 24-25 1.38490957119242 1105 26-27 1.3469250779052935 1105 28-29 0.9018271784783494 1105 30-31 0.6441653272306702 1105 32-33 0.5038765648751244 1105 34-35 0.48807163282822685 1105 36-37 0.3867114756562806 1105 38-39 0.3741560552415244 1105 40-41 0.5769231717949523 1105 42-43 0.47443727108787925 1105 44-45 0.5813048827241225 1105 46-47 0.6523308338611535 1105 48-49 0.6925651412490978 1105 50-51 0.5833096621898335 1105 52-53 0.5382343131119747 1105 54-55 0.18250052000353634 1105 56-57 0.28358589473373996 1105 58-59 0.447259084075192 1105 60-61 0.4723675679724799 1105 62-63 0.2999637903993495 1105 64-65 0.2710590747628032 1105 66-67 0.48305998378251047 1105 68-69 0.5000049400724187 1105 70-71 0.4625524108511847 1105 72-73 0.009832883893153621 1105 74-75 0.28140201846526836 1105 76-77 0.4156382853381899 1105 78-79 0.6365050563993293 1105 80-81 0.6328323329551999 1105 82-83 0.5404172674204624 1105 84-85 0.9225636966974236 1105 86-87 1.0390426749243105 1105 88-89 1.0638942542393686 1105 90-91 1.106136113353564 1105 92-93 1.209187958577818 1105 94-95 1.0691990820471204 1105 96-97 1.2496313545055493 1105 98-99 1.5161217129458713 1105 100-101 1.4059491232039996 1105 102-103 1.2031851058562104 1105 104-105 1.1253906711999804 1105 106-107 1.4205779950697384 1105 108-109 1.4725169132498621 1105 110-111 1.3787167419316404 1105 112-113 1.2927371223310722 1105 114-115 1.53617346992068 1105 116-117 1.420436106231385 1105 118-119 1.5461750814932316 1105 120-121 1.7676276797374193 1105 122-123 1.7693827683168237 1105 124-125 1.6991257010554897 1106 1 -0.24396530453897824 1106 2 0.6421992532348106 1106 3 1.135929536913597 1106 4 0.8736618877746203 1106 5 0.9297147849034815 1106 6 0.9308888817725531 1106 7 0.6625788142842275 1106 8 0.6748499011253202 1106 9 0.6056354806038904 1106 10-11 0.22485615738051834 1106 12-13 0.2940821091645347 1106 14-15 0.7064446417696644 1106 16-17 1.1841256396306967 1106 18-19 1.2816472118394522 1106 20-21 0.15739650944821193 1106 22-23 0.8773343208750966 1106 24-25 1.1739269122328828 1106 26-27 1.3064626501596273 1106 28-29 1.442289606224012 1106 30-31 1.6615063676931001 1106 32-33 1.3882696284589429 1106 34-35 1.0314242339842963 1106 36-37 1.2884455797025218 1106 38-39 0.7354277315421065 1106 40-41 0.6029347324885919 1106 42-43 0.971547097677476 1106 44-45 1.2084725127819276 1106 46-47 1.3777643598727174 1106 48-49 1.212796355121931 1106 50-51 1.4879339396464815 1106 52-53 1.6249395154241135 1106 54-55 1.855910924627814 1106 56-57 1.8963026577395183 1106 58-59 1.6726926100867523 1106 60-61 1.9376854870476201 1106 62-63 1.7681718828849 1106 64-65 2.0658567626240725 1106 66-67 2.089996399967479 1106 68-69 2.0173459805348486 1106 70-71 1.864286514897426 1106 72-73 1.9260178549914215 1106 74-75 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6 0.5269431667687847 2216 7 0.5805481533141332 2216 8 0.060197978531704166 2216 9 0.4155374660273665 2216 10-11 0.3462685725602128 2216 12-13 0.647123074231132 2216 14-15 0.1141098842929118 2216 16-17 0.5363749485022744 2216 18-19 0.013891494322489706 2216 20-21 0.542380173685455 2216 22-23 0.5145798599552656 2216 24-25 0.7685486764952607 2216 26-27 0.5895739846657868 2216 28-29 0.6265821420370301 2216 30-31 0.40424323625304126 2216 32-33 0.4916247428092788 2216 34-35 0.6001098333811292 2216 36-37 0.8649723452214992 2216 38-39 1.1829271032435393 2216 40-41 1.206365242667026 2216 42-43 0.9193731846339972 2216 44-45 0.750844968172725 2216 46-47 0.8370003488146587 2216 48-49 0.8620800947550009 2216 50-51 0.7810477873469104 2216 52-53 0.7287721366857376 2216 54-55 0.2543653101518153 2216 56-57 0.08225390177068448 2216 58-59 0.25311482672409724 2216 60-61 0.17114866875156665 2216 62-63 0.12303491379718778 2216 64-65 -0.1151434886953524 2216 66-67 0.18238142132963375 2216 68-69 0.06460659878063879 2216 70-71 0.32301986498539037 2216 72-73 0.27925987559961385 2216 74-75 0.5161217973041765 2216 76-77 0.5826651765168798 2216 78-79 0.19419543086527824 2216 80-81 0.17969084011780367 2216 82-83 0.40309633251219523 2216 84-85 0.4742098389442191 2216 86-87 0.14626810845032168 2216 88-89 0.43961679759197025 2216 90-91 0.12797577155914297 2216 92-93 0.1814925577233275 2216 94-95 0.13852554598278033 2216 96-97 0.0973946116053277 2216 98-99 -0.297989872879711 2216 100-101 -0.04129892907043953 2216 102-103 -0.4937990610198888 2216 104-105 -0.5297865089960538 2216 106-107 -0.6889093132991988 2216 108-109 -0.7834142252271441 2216 110-111 -0.8668977340673578 2216 112-113 -0.920180696216299 2216 114-115 -1.0866714659928682 2216 116-117 -0.8922932227457494 2216 118-119 -0.8760415558528365 2216 120-121 -0.8936188646204606 2216 122-123 -0.9692198956691023 2216 124-125 -0.7216584155567212 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 13924.0 3 3506.0 4 4580.0 5 3999.0 6 5659.0 7 3824.0 8 2740.0 9 1978.0 10 1980.0 11 1763.0 12 1384.0 13 1172.0 14 1251.0 15 1069.0 16 886.0 17 727.0 18 663.0 19 619.0 20 626.0 21 578.0 22 616.0 23 1693.0 24 6714.0 25 1960.0 26 5795.0 27 2976.0 28 2018.0 29 1417.0 30 1297.0 31 1470.0 32 2121.0 33 2204.0 34 3104.0 35 7676.0 36 6088.0 37 12074.0 38 11375.0 39 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.25274102844197 30.00364121859449 9.298053970951168 11.44556378201238 2 11.199385138141661 44.948019902107525 28.20516969378261 15.647425265968204 3 14.523801818711368 57.94068153134203 16.471958836283616 11.063557813662989 4 7.644873880216552 41.4370453092504 21.732093580312853 29.185987230220196 5 7.664794704559064 64.29137872436841 16.55796339154218 11.485863179530337 6 15.803611884264354 55.79001877144152 12.889992879798045 15.516376464496084 7 17.514905872549733 61.33614866231424 10.633529920960449 10.515415544175585 8 17.938699735481837 57.74908793813349 10.970627967740109 13.341584358644567 9 20.43395249498414 48.040579897741246 9.030968869328845 22.494498737945765 10-11 15.689145408730754 57.43748002960673 12.719977673605914 14.153396888056594 12-13 18.165050782055793 57.235886795047584 13.185942006123778 11.413120416772841 14-15 16.75195695705819 56.34944072493375 13.623252897510064 13.275349420497987 16-17 14.363255433947305 58.08640926703392 14.637480686938304 12.912854612080471 18-19 15.281249747186692 54.45679521717675 18.392673672629016 11.869281363007548 20-21 19.7803753437955 51.60815402038506 17.283611066170522 11.327859569648924 22-23 17.94274435573244 53.64824746604542 17.920498944354115 10.488509233868031 24-25 16.704959145700187 53.072162446404015 18.581020952997328 11.64185745489847 26-27 15.761885116606402 54.1056940163888 18.10816932398217 12.024251543022626 28-29 15.217936479686767 54.91004255181452 18.274629087320207 11.597391881178508 30-31 15.154787616990642 55.20663964860339 18.52274289966915 11.115829834736816 32-33 14.27392705902338 56.248356866377335 18.255062873876096 11.222653200723181 34-35 13.584261573680848 56.71083391980327 18.85197498806837 10.852929518447512 36-37 13.623806268682074 56.829902640871424 18.342912845984895 11.203378244461613 38-39 13.714902807775378 57.94727433041311 17.76235429255547 10.575468569256033 40-41 12.9206983592764 58.16640238179994 18.400699006504645 10.512200252419015 42-43 11.850530497004705 58.97193199606829 18.36534922195121 10.812188284975791 44-45 11.727828313265194 59.26808255912247 17.89711253392439 11.10697659368794 46-47 11.53013797818076 59.35124770946511 17.759988350127216 11.358625962226904 48-49 11.260763876249296 59.43398897423141 17.43292927086746 11.872317878651831 50-51 11.220370520184451 59.75730118922418 17.170536364371813 11.851791926219562 52-53 11.062124086380754 59.6041758517338 17.16168279867816 12.172017263207284 54-55 10.96370240339112 59.06954545086841 17.178866984314457 12.787885161426018 56-57 11.755307099864085 58.25634263154489 16.331062714387418 13.657287554203613 58-59 11.877859630811802 57.61819807355446 16.21054164593371 14.29340064970003 60-61 12.21363856981071 56.7080569487138 16.397427600711858 14.68087688076363 62-63 13.28476719399085 56.14930649656383 16.320083487377794 14.245842822067525 64-65 13.70900222052346 55.933327670796274 16.381719712504903 13.97595039617536 66-67 14.46489241223103 55.52297362886265 16.399449927196248 13.61268403171008 68-69 14.837689389343234 55.003599712023046 16.75645723623009 13.402253662403638 70-71 14.988067791125673 54.58318165271206 17.152044654774905 13.276705901387373 72-73 15.562080876226531 53.95563860509137 17.286706951084362 13.195573567597737 74-75 15.81374277994402 53.39929134240459 18.08209426116783 12.70487161648357 76-77 15.885646323999387 52.69176935088472 18.954036844958292 12.468547480157605 78-79 16.1122795664132 52.11535350266947 19.312004530011325 12.460362400906003 80-81 16.45573204224355 52.272552612313085 19.076741373522143 12.194973971921225 82-83 16.40383269765128 51.75719237505106 19.206765868120577 12.632209059177077 84-85 16.80701498936265 50.98365164494706 19.60184758253048 12.607485783159818 86-87 16.884944870934078 50.75473834928288 19.254726215225816 13.105590564557229 88-89 16.852591897488995 50.05015531969972 19.782228837690912 13.315023945120371 90-91 17.194989260968576 50.20203940476239 19.344817962294066 13.25815337197497 92-93 16.775548013640172 50.59685855402874 18.874160130414907 13.753433301916177 94-95 16.41185559087857 50.87687367033109 18.966842203185543 13.744428535604792 96-97 15.885034319019248 51.9646981261047 18.165418886178962 13.984848668697092 98-99 14.609641068785036 53.13913134924808 18.065071956122537 14.186155625844341 100-101 13.657064738191732 55.15203727522023 17.816956665938637 13.373941320649404 102-103 12.060248663253006 58.042727368327384 17.031895875296268 12.865128093123337 104-105 11.142031986926941 59.9580950223682 16.562982857790036 12.336890132914823 106-107 10.683459929947176 61.42767005605844 16.170391761917475 11.718478252076912 108-109 9.978887082291843 62.790302618487146 15.659556224266103 11.571254074954902 110-111 10.049344766219058 63.663646659116644 15.357951787736612 10.929056786927681 112-113 10.288924753364318 64.1940402949516 14.96155355199877 10.55548139968531 114-115 10.079638894843493 64.17382230293519 14.895992169583524 10.85054663263779 116-117 10.407616828855938 63.84311726971955 14.64314315528915 11.106122746135364 118-119 10.772329771140576 63.18793330798541 15.097926593481267 10.94181032739275 120-121 11.091471676744797 62.37911298966571 15.22721297550913 11.302202358080368 122-123 11.791281416587797 61.530403410423794 15.236084484027796 11.442230688960613 124-125 12.53561945469853 61.64634738682728 14.667120005181012 11.15091315329318 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11149.0 1 10259.0 2 9234.5 3 6317.5 4 2560.5 5 1211.0 6 748.5 7 773.0 8 1081.5 9 1215.5 10 804.0 11 383.0 12 301.0 13 267.0 14 260.0 15 246.0 16 218.0 17 229.5 18 236.0 19 257.5 20 309.5 21 361.0 22 401.0 23 480.0 24 610.0 25 721.0 26 872.5 27 1126.5 28 1382.0 29 1629.0 30 1941.5 31 2301.5 32 2555.0 33 2861.0 34 3454.5 35 3825.0 36 4031.5 37 4098.0 38 3881.5 39 3615.0 40 3359.5 41 3170.5 42 2884.5 43 2617.5 44 2372.5 45 2242.5 46 2068.5 47 1848.5 48 1812.5 49 1743.5 50 1685.0 51 1667.0 52 1515.0 53 1348.0 54 1247.5 55 1161.5 56 1067.0 57 939.0 58 817.5 59 799.0 60 851.5 61 815.0 62 636.5 63 379.5 64 230.5 65 207.5 66 196.0 67 169.0 68 130.5 69 100.5 70 106.5 71 95.0 72 108.0 73 159.0 74 131.0 75 55.5 76 35.5 77 44.5 78 36.0 79 15.0 80 10.5 81 10.0 82 9.0 83 8.5 84 9.5 85 9.0 86 7.0 87 3.5 88 2.0 89 2.5 90 2.0 91 2.0 92 1.0 93 2.0 94 3.0 95 1.5 96 1.5 97 2.0 98 3.5 99 6.5 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02912126580435363 2 0.012942784801934944 3 0.01375170885205588 4 0.038828354405804844 5 0.03478373415520017 6 0.02345879745350709 7 0.009707088601451211 8 0.0 9 0.010516012651572145 10-11 4.04462025060467E-4 12-13 0.0036401582255442036 14-15 0.019009715177841953 16-17 0.0 18-19 0.009707088601451211 20-21 8.08924050120934E-4 22-23 0.0 24-25 0.008898164551330275 26-27 0.0 28-29 0.005662468350846539 30-31 0.0 32-33 4.04462025060467E-4 34-35 0.0 36-37 0.0052580063257860726 38-39 0.0 40-41 0.01375170885205588 42-43 0.00849370252626981 44-45 4.04462025060467E-4 46-47 0.011729398726753545 48-49 0.0012133860751814014 50-51 0.008898164551330275 52-53 0.0044490822756651375 54-55 0.003235696200483736 56-57 0.010516012651572145 58-59 0.02062756327808382 60-61 8.08924050120934E-4 62-63 0.007684778476148874 64-65 0.0012133860751814014 66-67 8.08924050120934E-4 68-69 0.0 70-71 0.0048535443007256054 72-73 0.0 74-75 0.005662468350846539 76-77 0.01779632910266055 78-79 8.08924050120934E-4 80-81 0.0036401582255442036 82-83 4.04462025060467E-4 84-85 0.0 86-87 0.001617848100241868 88-89 0.00404462025060467 90-91 0.0052580063257860726 92-93 0.012538322776874479 94-95 0.0 96-97 0.0012133860751814014 98-99 0.003235696200483736 100-101 0.0 102-103 0.0 104-105 0.006471392400967472 106-107 0.0 108-109 0.0 110-111 8.08924050120934E-4 112-113 0.006066930375907006 114-115 4.04462025060467E-4 116-117 0.0 118-119 0.006471392400967472 120-121 0.0028312341754232694 122-123 0.0 124-125 0.07522993666124687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 123621.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.45195395604306 #Duplication Level Percentage of deduplicated Percentage of total 1 86.15968462205356 42.60764756796984 2 7.051837796280242 6.974543160142694 3 2.4389445962082674 3.6183172761909383 4 1.2906286293818396 2.5529643021816684 5 0.7557293115011532 1.868614555779358 6 0.46619665319876336 1.3832601257067973 7 0.31734087972126346 1.0985188600642286 8 0.2388235486562086 0.9448232905412511 9 0.21755843815942288 0.9682820879947582 >10 0.9373006395890926 8.181457842923127 >50 0.06215955375983511 2.0481956949062052 >100 0.04580177645461535 3.8529052507260095 >500 0.0065431109220879064 2.4979574667734443 >1k 0.00981466638313186 11.024016955048092 >5k 0.0 0.0 >10k+ 0.0016357777305219766 10.378495563051585 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12830 10.378495563051585 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4425 3.5794889217851336 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3339 2.700997403353799 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1817 1.4698149990697373 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1715 1.3873047459574022 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 1.0370406322550376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.8493702526269807 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 953 0.7709046197652503 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 909 0.7353119615599291 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 696 0.5630111388841702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.42872974656409507 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 395 0.319524999797769 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.2507664555374896 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.2394415188357965 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 289 0.23377905048494996 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.20465778468059634 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 211 0.17068297457551712 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.15126879737261467 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.14641525307188907 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 174 0.14075278472104255 No Hit CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC 171 0.13832601257067975 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.13185462016971225 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.12700107586898665 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.12457430371862385 No Hit GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.11972075941789825 No Hit GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.11648506321741452 No Hit GAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.11244044296680984 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 135 0.1092047467663261 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.10758689866608424 No Hit GTACATGGGGGGGAGGAAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.1019244303152377 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0446202506046707E-4 0.0 12-13 0.0 0.0 0.0 8.089240501209341E-4 0.0 14-15 0.0 0.0 0.0 8.089240501209341E-4 0.0 16-17 0.0 0.0 0.0 0.002022310125302335 0.0 18-19 0.0 0.0 0.0 0.0044490822756651375 0.0 20-21 0.0 0.0 0.0 0.006471392400967472 0.0 22-23 0.0 0.0 0.0 0.018605253152781484 0.0 24-25 0.0 0.0 0.0 0.04610867085689324 0.0 26-27 0.0 0.0 0.0 0.07078085438558174 0.0 28-29 0.0 0.0 0.0 0.11931629739283778 0.0 30-31 0.0 0.0 0.0 0.2430816770613407 0.0 32-33 0.0 0.0 0.0 0.39030585418335073 0.0 34-35 0.0 0.0 0.0 0.535103259154998 0.0 36-37 0.0 0.0 0.0 0.7078085438558174 0.0 38-39 0.0 0.0 0.0 0.9630240816689721 0.0 40-41 0.0 0.0 0.0 1.3096480371457924 0.0 42-43 0.0 0.0 0.0 1.5462583218061656 0.0 44-45 0.0 0.0 0.0 1.733524239409162 0.0 46-47 0.0 0.0 0.0 1.9442489544656651 0.0 48-49 0.0 0.0 0.0 2.1820726252012195 0.0 50-51 0.0 0.0 0.0 2.4215141440370163 0.0 52-53 0.0 0.0 0.0 2.6366879413691846 0.0 54-55 0.0 0.0 0.0 2.806561991894581 0.0 56-57 0.0 0.0 0.0 2.9861431310214286 0.0 58-59 0.0 0.0 0.0 3.1891830676017827 0.0 60-61 0.0 0.0 0.0 3.4253888902370955 0.0 62-63 0.0 0.0 0.0 3.6498653141456545 0.0 64-65 0.0 0.0 0.0 3.8254018330218975 0.0 66-67 0.0 0.0 0.0 4.030059617702493 0.0 68-69 0.0 0.0 0.0 4.248873573260207 0.0 70-71 0.0 0.0 0.0 4.514605123724934 0.0 72-73 0.0 0.0 0.0 4.763753731162181 0.0 74-75 0.0 0.0 0.0 5.009666642398946 0.0 76-77 0.0 0.0 0.0 5.232525218207263 0.0 78-79 0.0 0.0 0.0 5.494616610446445 0.0 80-81 0.0 0.0 0.0 5.770055249512623 0.0 82-83 0.0 0.0 0.0 6.065716989831825 0.0 84-85 0.0 0.0 0.0 6.371490280777538 0.0 86-87 0.0 0.0 0.0 6.656636008445167 0.0 88-89 0.0 0.0 0.0 6.97939670444342 0.0 90-91 0.0 0.0 0.0 7.310651102967942 0.0 92-93 0.0 0.0 0.0 7.656870596419703 0.0 94-95 0.0 0.0 0.0 8.003899013921583 0.0 96-97 0.0 0.0 0.0 8.351736355473584 0.0 98-99 0.0 0.0 0.0 8.712112019802461 0.0 100-101 0.0 0.0 0.0 9.111720500562203 0.0 102-103 0.0 0.0 0.0 9.490296956018799 0.0 104-105 0.0 0.0 0.0 9.900825911455172 0.0 106-107 0.0 0.0 0.0 10.321466417518058 0.0 108-109 0.0 0.0 0.0 10.67820192362139 0.0 110-111 0.0 0.0 0.0 11.124323537263086 0.0 112-113 0.0 0.0 0.0 11.570445150904781 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 175 0.0 98.678185 1 TACAAGG 110 0.0 92.027466 2 TACAAGA 80 0.0 89.320786 2 GAACAAA 710 0.0 77.15973 1 GTAACAA 80 0.0 74.43399 1 GTACATA 65 8.913048E-11 73.28884 1 CCTCATG 25 0.0020460447 71.45662 3 CGTCACT 25 0.0020460447 71.45662 1 GTACAAA 185 0.0 70.812874 1 GGTATCA 390 0.0 68.70829 1 TACATGA 85 1.4551915E-11 63.04996 2 GTATCAA 780 0.0 62.600887 1 GGAAACA 185 0.0 61.15657 1 GAATACA 40 1.758854E-4 59.547188 1 TACATAG 40 1.758854E-4 59.547188 2 GAAAACA 560 0.0 59.547188 1 TCGGCTC 15 0.0042396975 59.47492 84-85 TAACAAC 140 0.0 55.293816 1 GTGAGAA 45 3.1474943E-4 52.930832 1 CGCAGAA 115 5.456968E-12 51.78016 2 >>END_MODULE