##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940174_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 123621 Sequences flagged as poor quality 0 Sequence length 125 %GC 28 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40274710607421 33.0 31.0 34.0 31.0 34.0 2 32.416967990875335 33.0 31.0 34.0 31.0 34.0 3 32.45796426173546 34.0 31.0 34.0 30.0 34.0 4 36.01249787657437 37.0 35.0 37.0 35.0 37.0 5 36.09838943221621 37.0 35.0 37.0 35.0 37.0 6 36.26366070489642 37.0 37.0 37.0 35.0 37.0 7 36.22022957264542 37.0 37.0 37.0 35.0 37.0 8 36.24979574667734 37.0 37.0 37.0 35.0 37.0 9 37.948665679779324 39.0 39.0 39.0 35.0 39.0 10-11 37.95289635256146 39.0 39.0 39.0 35.0 39.0 12-13 37.934562088965464 39.0 38.5 39.0 35.0 39.0 14-15 38.834465827003505 40.0 38.0 41.0 35.5 41.0 16-17 38.92505723137654 40.0 38.5 41.0 35.5 41.0 18-19 39.30127567322704 41.0 39.0 41.0 35.5 41.0 20-21 39.27373989856092 41.0 39.0 41.0 35.0 41.0 22-23 38.98105095412592 41.0 38.5 41.0 35.0 41.0 24-25 38.79994903778484 41.0 37.5 41.0 35.0 41.0 26-27 38.37429724723145 41.0 36.0 41.0 35.0 41.0 28-29 37.98422598102264 40.5 35.0 41.0 35.0 41.0 30-31 36.80323326942833 38.0 35.0 40.5 33.5 41.0 32-33 35.385059981718314 35.0 35.0 40.0 33.0 41.0 34-35 34.66340670274468 35.0 35.0 40.0 31.0 41.0 36-37 34.14523017933846 35.0 35.0 40.0 29.0 41.0 38-39 33.7669044903374 35.0 35.0 39.0 25.0 41.0 40-41 33.35640384724278 35.0 35.0 39.0 22.0 41.0 42-43 32.68334263595991 35.0 34.5 39.0 15.0 41.0 44-45 31.66035705907572 35.0 33.0 39.5 3.5 41.0 46-47 29.458004707937974 35.0 27.0 39.5 2.0 41.0 48-49 27.495886621205134 35.0 17.5 39.0 2.0 41.0 50-51 27.16100824293607 35.0 11.0 39.0 2.0 41.0 52-53 27.399814756392523 35.0 10.0 39.0 2.0 41.0 54-55 27.433611603206575 35.0 10.0 39.0 2.0 41.0 56-57 27.399074590886663 35.0 10.0 38.0 2.0 41.0 58-59 27.296964108039894 35.0 9.0 37.5 2.0 41.0 60-61 27.137589891685067 35.0 4.5 37.0 2.0 41.0 62-63 26.90653287062878 35.0 2.0 37.0 2.0 41.0 64-65 26.57933118159536 35.0 2.0 36.0 2.0 41.0 66-67 26.180992711594307 35.0 2.0 36.0 2.0 41.0 68-69 25.828313959602333 35.0 2.0 35.0 2.0 40.5 70-71 25.50095453037914 35.0 2.0 35.0 2.0 40.0 72-73 25.2585442602794 35.0 2.0 35.0 2.0 39.5 74-75 25.06496469046521 35.0 2.0 35.0 2.0 39.0 76-77 24.85847873743134 35.0 2.0 35.0 2.0 39.0 78-79 24.653703658763476 35.0 2.0 35.0 2.0 38.0 80-81 24.401962449745593 35.0 2.0 35.0 2.0 37.0 82-83 24.16347546128894 35.0 2.0 35.0 2.0 37.0 84-85 23.935937259850675 35.0 2.0 35.0 2.0 36.0 86-87 23.723703901440693 35.0 2.0 35.0 2.0 36.0 88-89 23.506847542084273 35.0 2.0 35.0 2.0 36.0 90-91 23.296147094749273 35.0 2.0 35.0 2.0 35.0 92-93 23.083521408174988 34.5 2.0 35.0 2.0 35.0 94-95 22.871850251979843 34.0 2.0 35.0 2.0 35.0 96-97 22.663447148947185 34.0 2.0 35.0 2.0 35.0 98-99 22.4678776259697 34.0 2.0 35.0 2.0 35.0 100-101 22.296134960888523 34.0 2.0 35.0 2.0 35.0 102-103 21.579501055645885 32.0 2.0 35.0 2.0 35.0 104-105 21.859651677304015 33.0 2.0 35.0 2.0 35.0 106-107 21.701632408733147 33.0 2.0 35.0 2.0 35.0 108-109 21.47438946457317 33.0 2.0 35.0 2.0 35.0 110-111 21.248287103323868 33.0 2.0 35.0 2.0 35.0 112-113 20.976727255078018 33.0 2.0 35.0 2.0 35.0 114-115 20.640785141683047 33.0 2.0 35.0 2.0 35.0 116-117 20.31258847606798 32.5 2.0 35.0 2.0 35.0 118-119 20.032037437005037 32.0 2.0 35.0 2.0 35.0 120-121 19.716646039103388 31.0 2.0 35.0 2.0 35.0 122-123 19.392963169687995 30.5 2.0 35.0 2.0 35.0 124-125 18.59785149772288 26.5 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5059997318337253 1101 2 0.25864948816190036 1101 3 0.3566715988624978 1101 4 0.013914538229634843 1101 5 0.13212794939764194 1101 6 0.2841101374083479 1101 7 0.2936454349033397 1101 8 0.4326692029058279 1101 9 0.5313859672736001 1101 10-11 0.4898964129746304 1101 12-13 0.35924447041698926 1101 14-15 0.43312243158169395 1101 16-17 0.40628441986294206 1101 18-19 0.30315663408904925 1101 20-21 0.4954222842335554 1101 22-23 0.5205751117121267 1101 24-25 0.6175466264858471 1101 26-27 0.6291446490637753 1101 28-29 0.553011438025294 1101 30-31 0.4325601413643767 1101 32-33 0.23281863362520028 1101 34-35 -0.31498837165765536 1101 36-37 0.32774654832050487 1101 38-39 0.09643902172607 1101 40-41 0.039673210649866064 1101 42-43 0.6245579039763882 1101 44-45 0.8304288803227777 1101 46-47 0.7967627916062092 1101 48-49 0.5618138751386716 1101 50-51 0.3359249410914984 1101 52-53 -0.0768713092021649 1101 54-55 -0.06795085117218491 1101 56-57 0.11842659846855952 1101 58-59 0.12508990952002463 1101 60-61 0.31791901908618314 1101 62-63 0.6086124617422541 1101 64-65 0.49112596424581056 1101 66-67 0.6321258052439767 1101 68-69 0.7592537034742577 1101 70-71 1.0098955464997665 1101 72-73 0.9748429426391247 1101 74-75 1.085632613277145 1101 76-77 1.2548531584560259 1101 78-79 1.2331014542053573 1101 80-81 1.2709157599655754 1101 82-83 1.179449606274357 1101 84-85 1.4164735389916316 1101 86-87 1.4042497361836261 1101 88-89 1.362611762534378 1101 90-91 1.4770933876271783 1101 92-93 1.5939030459150914 1101 94-95 1.7892049696626486 1101 96-97 2.091488060152944 1101 98-99 2.0375239921728934 1101 100-101 1.9747478789991462 1101 102-103 2.0943862753871585 1101 104-105 1.5086296461671154 1101 106-107 1.4835340696605357 1101 108-109 1.6082534058080853 1101 110-111 1.84974489457203 1101 112-113 1.5632165487601526 1101 114-115 1.69457920494672 1101 116-117 1.5990544235379929 1101 118-119 1.5549025488196584 1101 120-121 1.6345246608614623 1101 122-123 1.9008808000669646 1101 124-125 1.5643558191916789 1102 1 -0.055221028400190164 1102 2 0.19848866944845156 1102 3 -0.09285325493866736 1102 4 0.01735021074425447 1102 5 -0.21143930206434902 1102 6 0.03155165787618586 1102 7 0.1888939729150323 1102 8 0.020023004075419237 1102 9 0.16124707838471153 1102 10-11 0.1864972901676154 1102 12-13 0.20312239439359558 1102 14-15 0.16276424444718884 1102 16-17 0.07291453682200455 1102 18-19 0.0855944995861222 1102 20-21 0.1323009392043133 1102 22-23 0.3010758427062825 1102 24-25 0.2856021820414014 1102 26-27 -0.024418947427456317 1102 28-29 -0.007478328056578221 1102 30-31 0.05783426417139026 1102 32-33 0.31470825350823617 1102 34-35 0.46154671606164044 1102 36-37 0.763821109724006 1102 38-39 0.8550282030126226 1102 40-41 0.8407148773165432 1102 42-43 0.8940023484208339 1102 44-45 1.1736306347087435 1102 46-47 0.8730420313722931 1102 48-49 1.6079944306942302 1102 50-51 1.3073613446002739 1102 52-53 1.2364327843650855 1102 54-55 1.369677072804425 1102 56-57 1.5812920955445833 1102 58-59 1.2290921025024808 1102 60-61 0.7846039606066526 1102 62-63 1.1294640699293872 1102 64-65 1.3222297654154005 1102 66-67 1.6437668871153228 1102 68-69 1.8408143759888773 1102 70-71 1.4581411605348542 1102 72-73 1.358706246732691 1102 74-75 1.1569228179555076 1102 76-77 1.1812420473449166 1102 78-79 1.2468624191176367 1102 80-81 1.244508596222886 1102 82-83 1.5287551618299133 1102 84-85 1.2988565799273069 1102 86-87 1.3535370168853795 1102 88-89 1.1419611777390593 1102 90-91 1.0642461654049562 1102 92-93 1.2346742447454986 1102 94-95 1.2061457591363336 1102 96-97 1.3449054578137627 1102 98-99 1.2004443138103227 1102 100-101 1.0890205398178594 1102 102-103 0.41553028123511027 1102 104-105 0.8349637104946055 1102 106-107 1.0341736895435716 1102 108-109 1.3698944876794314 1102 110-111 1.474671795156823 1102 112-113 1.4696492972981652 1102 114-115 1.6055989417888235 1102 116-117 1.4529286925438392 1102 118-119 1.4119383675331072 1102 120-121 1.1914508304521085 1102 122-123 0.7705628176108235 1102 124-125 0.3081789186068882 1103 1 0.14626135974069854 1103 2 0.07289367420840875 1103 3 -0.07167142439331542 1103 4 0.11594942195056035 1103 5 0.1205000424209004 1103 6 0.17509850950137462 1103 7 -0.01129642556178112 1103 8 0.09982036569652308 1103 9 0.0210661998317363 1103 10-11 0.07521618041648992 1103 12-13 0.0628055169286128 1103 14-15 0.0783259199537838 1103 16-17 3.251175373648607E-4 1103 18-19 0.09785140153091021 1103 20-21 0.26068391214053577 1103 22-23 0.1889617396191099 1103 24-25 0.22132569625328813 1103 26-27 0.12194988162191578 1103 28-29 0.19036608918808895 1103 30-31 0.03539444368995959 1103 32-33 -0.27641101753759756 1103 34-35 -0.34144186002975374 1103 36-37 -0.07879414935391793 1103 38-39 0.12187506823769922 1103 40-41 0.031606350184759435 1103 42-43 0.16176720630197394 1103 44-45 -0.671024608049315 1103 46-47 -1.3411732549054172 1103 48-49 -1.334189031838072 1103 50-51 -1.2817349426294307 1103 52-53 -1.0978015417603046 1103 54-55 -1.5090118976838127 1103 56-57 -1.4059920061826077 1103 58-59 -1.389226950945094 1103 60-61 -1.7227059809138154 1103 62-63 -1.6811404452344902 1103 64-65 -1.6212286869169787 1103 66-67 -1.2791387296397438 1103 68-69 -1.1768654825722535 1103 70-71 -1.5639416628025593 1103 72-73 -1.8823518248027362 1103 74-75 -1.6052830843972714 1103 76-77 -1.6785770741021118 1103 78-79 -1.7313607550969685 1103 80-81 -1.4561917981739612 1103 82-83 -1.4802887658186705 1103 84-85 -1.085561344729296 1103 86-87 -1.1811427056768373 1103 88-89 -1.0474173072330615 1103 90-91 -0.7978339379542199 1103 92-93 -1.2346580005965393 1103 94-95 -0.6748211931280501 1103 96-97 -1.0821311258935644 1103 98-99 -0.7506300775945505 1103 100-101 -0.4359352605357394 1103 102-103 -0.32792477112447216 1103 104-105 -0.5261814003445124 1103 106-107 -0.45229441871155984 1103 108-109 -0.18963903605237675 1103 110-111 -0.09298766356750576 1103 112-113 0.27251887434154654 1103 114-115 0.44770420494671725 1103 116-117 0.38481023749148235 1103 118-119 0.3921845255638452 1103 120-121 -0.13618754844086212 1103 122-123 -0.01212792086326786 1103 124-125 -0.24916162266878317 1104 1 0.12021756809103579 1104 2 0.286207967694061 1104 3 0.04164966903793754 1104 4 -0.03528136820311545 1104 5 0.18037356343273103 1104 6 0.18359844150191407 1104 7 0.07778286180392513 1104 8 0.25394113273038954 1104 9 0.18463889125020216 1104 10-11 0.3560879334424669 1104 12-13 0.3200814587210772 1104 14-15 0.0224133672542024 1104 16-17 -0.017728738031799196 1104 18-19 -0.03428854134954662 1104 20-21 -0.15424876839801982 1104 22-23 -0.05272532688436371 1104 24-25 0.06922791303555442 1104 26-27 0.19780327479477222 1104 28-29 0.1972000345165199 1104 30-31 -0.33105462471749547 1104 32-33 0.136345680408823 1104 34-35 -0.12324860557578887 1104 36-37 -0.25664672653330456 1104 38-39 -0.44029343441427926 1104 40-41 -0.24408044432088616 1104 42-43 -0.3808514527487503 1104 44-45 -0.11876702610997114 1104 46-47 0.270702850085744 1104 48-49 -0.5089646336332585 1104 50-51 -0.09614742732955506 1104 52-53 0.6428655329030981 1104 54-55 0.8462852599389272 1104 56-57 0.8970815692287921 1104 58-59 0.4922499972393233 1104 60-61 0.6413291068054825 1104 62-63 0.7288792746077526 1104 64-65 0.7579022800352817 1104 66-67 0.9741762438404713 1104 68-69 0.6858436157549583 1104 70-71 0.7446908681371944 1104 72-73 1.0780044923467251 1104 74-75 1.034115800411648 1104 76-77 1.1110666087484269 1104 78-79 1.048032009760913 1104 80-81 0.6860290640591415 1104 82-83 1.01121130218079 1104 84-85 1.0824823109214599 1104 86-87 1.2628937420315793 1104 88-89 1.5074582537624472 1104 90-91 1.236760785287995 1104 92-93 0.8428613792484256 1104 94-95 0.7295375720018313 1104 96-97 0.6957826507962217 1104 98-99 1.0045378810617827 1104 100-101 1.2176755105780934 1104 102-103 1.079272971293591 1104 104-105 0.7092327163425551 1104 106-107 0.5224777831108227 1104 108-109 0.3114149555156871 1104 110-111 0.5214554208878184 1104 112-113 0.6216960809238969 1104 114-115 0.24887379558999356 1104 116-117 0.005560271491209079 1104 118-119 0.025973455252408684 1104 120-121 -0.11264273679935499 1104 122-123 -0.22943718238917654 1104 124-125 0.024553187612735172 1105 1 0.015934702931993172 1105 2 0.0643575806474459 1105 3 0.105334893660185 1105 4 -0.1334120513657453 1105 5 -0.5918604899087185 1105 6 0.04530955937366343 1105 7 -0.06466381365157758 1105 8 -0.2143683693485059 1105 9 -0.05391027550096794 1105 10-11 0.10105970777231477 1105 12-13 0.054168603364963985 1105 14-15 0.2867712755123222 1105 16-17 0.21759222333114536 1105 18-19 0.3845330791757533 1105 20-21 0.2835364460832608 1105 22-23 0.011669764891315992 1105 24-25 0.06530674209278686 1105 26-27 0.28974797276320174 1105 28-29 0.255865484268071 1105 30-31 0.3600348523470345 1105 32-33 0.03236704402982582 1105 34-35 -0.17271958553049416 1105 36-37 0.10278267548812892 1105 38-39 -0.3600277412797084 1105 40-41 -0.392425777789434 1105 42-43 0.3166099270977796 1105 44-45 -0.026146264185893386 1105 46-47 -0.5240103297810705 1105 48-49 0.023537141034626785 1105 50-51 0.0955167040972782 1105 52-53 0.597839673456793 1105 54-55 0.8016481372671223 1105 56-57 0.748195384595725 1105 58-59 0.563186007785923 1105 60-61 0.7440570248665317 1105 62-63 0.33843543862086634 1105 64-65 0.4520002417024571 1105 66-67 0.8230759497526456 1105 68-69 0.9530578942257009 1105 70-71 1.171456240141385 1105 72-73 1.211746844373227 1105 74-75 1.0488370930459325 1105 76-77 1.179636395450249 1105 78-79 1.3681989975579576 1105 80-81 1.599763303318177 1105 82-83 1.591696716100948 1105 84-85 1.8610544638471254 1105 86-87 1.9406478575709087 1105 88-89 1.5399600284303325 1105 90-91 1.3962949772225564 1105 92-93 1.3361176412908122 1105 94-95 1.538211472552824 1105 96-97 1.4350754878986116 1105 98-99 1.8266316511324305 1105 100-101 1.926680682467353 1105 102-103 2.2406832407050743 1105 104-105 2.2960393282480425 1105 106-107 1.7807342141692004 1105 108-109 1.8035388104323644 1105 110-111 1.727418304976652 1105 112-113 1.8377830227485923 1105 114-115 2.0083978465652166 1105 116-117 2.0082307241160287 1105 118-119 1.9131214794554978 1105 120-121 1.6468982157747583 1105 122-123 1.6737673607606034 1105 124-125 2.098785009943125 1106 1 -0.1112329271258119 1106 2 0.018114806080973267 1106 3 -0.021832736397612962 1106 4 0.09202147464581856 1106 5 -0.08318996967750536 1106 6 -0.09919911114656799 1106 7 0.11452693779351364 1106 8 -0.15656490114041333 1106 9 0.038575273631977325 1106 10-11 0.04036606615381544 1106 12-13 0.1784460600123552 1106 14-15 -0.11207265512351228 1106 16-17 -0.0829858113509303 1106 18-19 -0.22240333700921155 1106 20-21 -0.05461384293407434 1106 22-23 -0.15017994609134178 1106 24-25 0.02484431434711354 1106 26-27 0.24639537160712877 1106 28-29 0.4610677964067946 1106 30-31 0.4496302280695801 1106 32-33 0.5901705122379184 1106 34-35 0.3937544029088116 1106 36-37 0.28197342693322014 1106 38-39 0.1341919118994781 1106 40-41 -0.29705005524608197 1106 42-43 -0.0533322694340157 1106 44-45 -0.017475743954680212 1106 46-47 0.7881283985426286 1106 48-49 -0.09785014220236476 1106 50-51 -0.07211335370618599 1106 52-53 0.23945817056662122 1106 54-55 0.07910478466596516 1106 56-57 0.3695826678327201 1106 58-59 0.5602958343755162 1106 60-61 0.5244038456757778 1106 62-63 0.5089556698347373 1106 64-65 0.5560464844770223 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-0.3656675140150618 2216 66-67 -0.4068687599734133 2216 68-69 -0.06099086174313584 2216 70-71 -0.03358271436980331 2216 72-73 -0.10328205736087526 2216 74-75 -0.15118803889676968 2216 76-77 -0.07938597197875552 2216 78-79 -0.2640452849250785 2216 80-81 -0.08465489220833788 2216 82-83 0.20146047583957483 2216 84-85 -0.09276559144314689 2216 86-87 -0.3744187420772427 2216 88-89 -0.17896432442214305 2216 90-91 -0.3852707428076023 2216 92-93 -0.21438499756317242 2216 94-95 -0.09190916077213274 2216 96-97 -0.04234346158618507 2216 98-99 -0.07565535565319337 2216 100-101 0.19879679204262146 2216 102-103 0.20430475364802447 2216 104-105 0.14409160268885302 2216 106-107 -0.14921049555685784 2216 108-109 -0.77204550723539 2216 110-111 -0.5931898880366617 2216 112-113 -0.3802617121094123 2216 114-115 -0.47186101244458456 2216 116-117 -0.569967315592443 2216 118-119 -0.592244190310776 2216 120-121 -0.48843729566027605 2216 122-123 -0.7435485477591222 2216 124-125 -1.1065680938517986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 61.0 6 220.0 7 348.0 8 623.0 9 967.0 10 1634.0 11 2236.0 12 2651.0 13 4106.0 14 7512.0 15 8108.0 16 2511.0 17 2217.0 18 1471.0 19 1142.0 20 1197.0 21 1385.0 22 1508.0 23 1033.0 24 1269.0 25 1404.0 26 1547.0 27 1728.0 28 1884.0 29 2039.0 30 2344.0 31 2814.0 32 3304.0 33 3692.0 34 6088.0 35 23050.0 36 6035.0 37 12417.0 38 12948.0 39 117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.434312622883176 17.517173302695134 17.62073903861869 10.427775035802998 2 16.797987315557858 25.475666580377943 43.24359306238675 14.482753041677455 3 14.75767896001359 38.91535953792763 36.39062588478931 9.936335617269469 4 7.537700645140401 24.707986951489 36.65644047628684 31.09787192708375 5 4.915990519103359 39.13458504898193 33.614308711584975 22.33511572032973 6 15.529950561143163 45.81549839384401 16.240381269874664 22.414169775138163 7 11.442230688960613 48.49661465285025 22.450069163006287 17.611085495182856 8 23.759717200152078 31.14762054990657 19.877690683621715 25.214971566319637 9 27.464589349705953 12.817401574166201 22.30688960613488 37.41111946999297 10-11 20.493686347788806 30.849532037437005 24.511612104739484 24.1451695100347 12-13 30.04707937971704 32.300741783353956 28.609621342652137 9.042557494276862 14-15 22.883247991846044 26.199432135316812 40.57077680976533 10.346543063071808 16-17 8.577426165457325 29.897428430444666 40.89839105006431 20.6267543540337 18-19 8.475501735142089 15.139418060038343 62.790707080512206 13.59437312430736 20-21 9.959068443063881 13.816827238090616 67.15444786888959 9.069656449955914 22-23 9.107271418286537 15.314550116889524 68.20483574797163 7.3733427168523145 24-25 8.800724798880445 15.164151576801583 68.49187634736957 7.543247276948403 26-27 8.450020627563278 15.359445401671238 68.25134888085358 7.939185089911908 28-29 8.079631449730828 15.090256796055638 68.1745193921671 8.655592362046441 30-31 9.331796303037656 14.608259515430975 68.14666504874005 7.913279132791327 32-33 8.072657558181863 15.177437490394027 68.31848957701362 8.431415374410497 34-35 8.36508360230058 14.85791249059626 68.4341657161809 8.342838190922254 36-37 8.391407573986514 14.535210584005078 67.23116311614983 9.842218725858576 38-39 9.05711812717904 14.206324168223846 66.65776850211533 10.07878920248178 40-41 8.808373981766852 14.161428883442134 66.43046084403136 10.59973629075966 42-43 8.878345912102313 14.366491130147791 65.36146771179654 11.393695245953356 44-45 8.816712844055251 15.005561510303961 63.12455760713491 13.053168038505875 46-47 10.307839782558577 14.88721439578707 59.45704358940135 15.347902232253002 48-49 10.551200847752403 16.00213555949232 55.08246980690983 18.364193785845448 50-51 10.282762832724611 17.488745707594674 52.43509316895797 19.79339829072274 52-53 9.942485500036401 18.20079112772102 51.71936806853205 20.137355303710535 54-55 9.727547768124383 17.90556391464026 50.797213997961464 21.569674319273894 56-57 10.584814007385507 17.35149105528614 49.20502667437844 22.858668262949916 58-59 10.338579269451827 17.48793677372906 49.96096894098423 22.21251501583488 60-61 11.486721511717265 17.493791507915322 49.897266645634645 21.122220334732773 62-63 12.617601139001916 18.096216539796306 50.37454395431049 18.911638366891285 64-65 12.761387812553087 18.493516368842975 51.293894951423326 17.45120086718061 66-67 12.387458441526924 18.69504372234491 52.311500473220576 16.605997362907594 68-69 12.459503075946142 18.439902766935905 52.6761338127576 16.424460344360362 70-71 12.036126986438225 18.817015114929276 52.6223613588471 16.524496539785392 72-73 11.659568271995923 18.627320123443308 52.61629435485502 17.09681724970575 74-75 11.252537999207254 18.681292013492854 53.00677069429951 17.05939929300038 76-77 10.83773529958987 18.723658984460318 53.74780575801454 16.69079995793527 78-79 10.728846464973305 18.84363371622715 53.82907296553956 16.59844685325999 80-81 10.652316354017522 19.376562234571796 53.026589333527475 16.944532077883206 82-83 10.417770515408044 19.556222471192076 52.81850502141635 17.20750199198353 84-85 10.74655600585661 19.12053777270852 52.37661885925531 17.756287362179567 86-87 10.818364565174306 19.40486254080093 51.57156898037106 18.2052039136537 88-89 10.875438851947127 19.18045915643353 51.632043877105275 18.31205811451407 90-91 11.10562824842761 19.575302849515644 51.09025825631485 18.228810645741905 92-93 11.145671122616134 19.96845106882116 50.07219851558234 18.81367929298036 94-95 11.40241954076453 19.877688210098 49.5484090164498 19.171483232687663 96-97 11.636748990834594 20.143669560011972 48.665636603379795 19.553944845773643 98-99 11.704795495728709 20.420091250323583 48.177824877038574 19.69728837690914 100-101 12.07110643719538 20.85991061136166 47.88197463951301 19.187008311929947 102-103 11.871200508014578 21.399709590393023 47.07264689346659 19.656443008125805 104-105 12.14483034771741 21.58379880359649 46.6929028761411 19.578467972545006 106-107 12.349843473196302 21.871688467169818 46.283802913744424 19.494665145889453 108-109 12.227628859307437 22.35030963446549 45.83196819117653 19.59009331505054 110-111 11.977430836434234 22.995469988674973 45.664536482769776 19.362562692121017 112-113 12.274861772307542 23.373928659545296 45.32857137078997 19.022638197357193 114-115 11.826065150742997 23.530791693967853 45.26617645869229 19.376966696596856 116-117 12.04290550515489 23.406716523553943 45.09769819730546 19.452679773985707 118-119 12.003672530041538 23.53897614068864 45.38688971489356 19.070461614376256 120-121 11.835772239344449 23.679633719190104 45.567500667362346 18.917093374103107 122-123 11.703607830448147 23.966186701181037 45.069568031062936 19.260637437307878 124-125 11.550507421135265 23.956023501610478 44.78497321269605 19.708495864558213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3299.0 1 2315.5 2 2616.5 3 3161.0 4 1430.0 5 699.5 6 1745.5 7 4531.5 8 7638.5 9 8054.5 10 4673.5 11 1402.5 12 705.5 13 567.0 14 559.5 15 546.0 16 531.5 17 584.0 18 606.0 19 597.0 20 634.5 21 628.5 22 630.0 23 698.5 24 805.5 25 884.0 26 948.0 27 1069.0 28 1298.0 29 1555.5 30 1764.0 31 1992.5 32 2247.5 33 2524.0 34 3078.5 35 3521.0 36 3845.5 37 4071.5 38 3977.0 39 3859.5 40 3738.0 41 3574.0 42 3151.0 43 2734.0 44 2472.0 45 2256.0 46 2047.0 47 1915.5 48 1904.0 49 1821.5 50 1695.5 51 1686.5 52 1476.5 53 1092.0 54 924.5 55 893.5 56 831.5 57 674.0 58 503.5 59 517.0 60 581.5 61 538.0 62 454.5 63 286.0 64 157.0 65 140.5 66 125.5 67 104.5 68 77.5 69 63.0 70 73.5 71 69.0 72 67.0 73 100.0 74 87.0 75 33.5 76 20.5 77 35.5 78 29.0 79 5.5 80 3.5 81 3.0 82 2.0 83 2.5 84 2.5 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022649873403386155 2 0.00404462025060467 3 0.003235696200483736 4 0.0663317721099166 5 0.003235696200483736 6 0.02750341770411176 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.04462025060467E-4 26-27 0.0 28-29 0.0012133860751814014 30-31 0.0048535443007256054 32-33 0.0 34-35 0.0 36-37 4.04462025060467E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0028312341754232694 46-47 0.0012133860751814014 48-49 0.0 50-51 0.0012133860751814014 52-53 0.0 54-55 0.0024267721503628027 56-57 4.04462025060467E-4 58-59 0.0012133860751814014 60-61 0.0 62-63 0.003235696200483736 64-65 0.001617848100241868 66-67 0.0 68-69 4.04462025060467E-4 70-71 0.0012133860751814014 72-73 0.0012133860751814014 74-75 0.0 76-77 0.001617848100241868 78-79 8.08924050120934E-4 80-81 0.0 82-83 4.04462025060467E-4 84-85 0.0 86-87 0.002022310125302335 88-89 0.0024267721503628027 90-91 0.0028312341754232694 92-93 0.0028312341754232694 94-95 0.002022310125302335 96-97 0.003235696200483736 98-99 0.00404462025060467 100-101 0.0028312341754232694 102-103 0.002022310125302335 104-105 4.04462025060467E-4 106-107 0.0 108-109 0.006066930375907006 110-111 8.08924050120934E-4 112-113 0.002022310125302335 114-115 0.0 116-117 4.04462025060467E-4 118-119 4.04462025060467E-4 120-121 0.0 122-123 8.08924050120934E-4 124-125 0.04449082275665138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 123621.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.8769302950146 #Duplication Level Percentage of deduplicated Percentage of total 1 81.12419112204208 27.48238567880862 2 8.899448410898064 6.029719869601443 3 3.366842570261945 3.4217487320115514 4 1.7765467179254517 2.4073579731599 5 1.0936268774326035 1.8524360747769393 6 0.723512977864801 1.4706239231198581 7 0.5038324697342343 1.1947808220286198 8 0.40831920532964017 1.106608100565438 9 0.26504930872274884 0.8081151260708133 >10 1.5855201891162636 9.797688095064755 >50 0.11700374889562787 2.612824681890617 >100 0.10267675923493874 6.773121071662581 >500 0.011939158050574274 3.035083036053745 >1k 0.014326989660689128 11.159107271418286 >5k 0.004775663220229709 12.191294359372598 >10k+ 0.0023878316101148545 8.657105184394236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10702 8.657105184394236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8789 7.10963347651289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6282 5.081660882859708 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3983 3.221944491631681 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2882 2.331319112448532 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2232 1.805518479869925 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 1.7852953786169017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 1.0370406322550376 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1209 0.9779891765962093 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 858 0.6940568350037615 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.67545158185098 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 831 0.6722158856504963 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 667 0.5395523414306631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 561 0.4538063921178441 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 459 0.3712961390055088 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 427 0.3454105694016389 RNA PCR Primer, Index 17 (95% over 21bp) CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 353 0.28555018969268975 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 0.25723784793845705 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.24672183528688493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 299 0.24186829098615933 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 280 0.2264987340338616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 277 0.22407196188349876 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.22164518973313596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 261 0.2111291770815638 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 259 0.20951132898132194 RNA PCR Primer, Index 17 (95% over 23bp) TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.20627563278083821 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 248 0.20061316442999166 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 215 0.17391867077600084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 213 0.17230082267575897 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.14965094927237282 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTC 184 0.14884202522225187 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 178 0.14398848092152627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT 170 0.1375170885205588 No Hit CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC 159 0.12861892396922853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG 157 0.12700107586898665 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 154 0.12457430371862385 RNA PCR Primer, Index 17 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 154 0.12457430371862385 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 152 0.12295645561838199 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 149 0.12052968346801919 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG 148 0.11972075941789825 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 144 0.11648506321741452 No Hit GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 141 0.1140582910670517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 138 0.1116315189166889 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 137 0.11082259486656798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 134 0.10839582271620518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 134 0.10839582271620518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 132 0.1067779746159633 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 126 0.1019244303152377 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT 126 0.1019244303152377 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.1019244303152377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 125 0.10111550626511676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA 124 0.10030658221499583 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 124 0.10030658221499583 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0446202506046707E-4 0.0 12-13 0.0 0.0 0.0 8.089240501209341E-4 0.0 14-15 0.0 0.0 0.0 8.089240501209341E-4 0.0 16-17 0.0 0.0 0.0 0.002022310125302335 0.0 18-19 0.0 0.0 0.0 0.0044490822756651375 0.0 20-21 0.0 0.0 0.0 0.006471392400967472 0.0 22-23 0.0 0.0 0.0 0.018605253152781484 0.0 24-25 0.0 0.0 0.0 0.04570420883183278 0.0 26-27 0.0 0.0 0.0 0.06916300628533986 0.0 28-29 0.0 0.0 0.0 0.11729398726753545 0.0 30-31 0.0 0.0 0.0 0.2430816770613407 0.0 32-33 0.0 0.0 0.0 0.39192370228359263 0.0 34-35 0.0 0.0 0.0 0.5363166452301793 0.0 36-37 0.0 0.0 0.0 0.7090219299309988 0.0 38-39 0.0 0.0 0.0 0.9634285436940325 0.0 40-41 0.0 0.0 0.0 1.3108614232209739 0.0 42-43 0.0 0.0 0.0 1.5519207901570122 0.0 44-45 0.0 0.0 0.0 1.7399956318101293 0.0 46-47 0.0 0.0 0.0 1.9535515810420558 0.0 48-49 0.0 0.0 0.0 2.197037720128457 0.0 50-51 0.0 0.0 0.0 2.443759555415342 0.0 52-53 0.0 0.0 0.0 2.6698538274241432 0.0 54-55 0.0 0.0 0.0 2.856715283002079 0.0 56-57 0.0 0.0 0.0 3.0553061373067685 0.0 58-59 0.0 0.0 0.0 3.2870628776664157 0.0 60-61 0.0 0.0 0.0 3.5552212002815056 0.0 62-63 0.0 0.0 0.0 3.809627814044539 0.0 64-65 0.0 0.0 0.0 4.020352529101043 0.0 66-67 0.0 0.0 0.0 4.270714522613472 0.0 68-69 0.0 0.0 0.0 4.538468383203501 0.0 70-71 0.0 0.0 0.0 4.851117528575243 0.0 72-73 0.0 0.0 0.0 5.164980060022165 0.0 74-75 0.0 0.0 0.0 5.4654953446420915 0.0 76-77 0.0 0.0 0.0 5.74942768623454 0.0 78-79 0.0 0.0 0.0 6.073401768307973 0.0 80-81 0.0 0.0 0.0 6.410723097208403 0.0 82-83 0.0 0.0 0.0 6.760987210910768 0.0 84-85 0.0 0.0 0.0 7.1027576220868625 0.0 86-87 0.0 0.0 0.0 7.435225406686566 0.0 88-89 0.0 0.0 0.0 7.792365374814958 0.0 90-91 0.0 0.0 0.0 8.175795374572282 0.0 92-93 0.0 0.0 0.0 8.548709361678032 0.0 94-95 0.0 0.0 0.0 8.931734899410294 0.0 96-97 0.0 0.0 0.0 9.313142589042315 0.0 98-99 0.0 0.0 0.0 9.702639519175545 0.0 100-101 0.0 0.0 0.0 10.128133569539155 0.0 102-103 0.0 0.0 0.0 10.532191132574564 0.0 104-105 0.0 0.0 0.0 10.975077050015773 0.0 106-107 0.0 0.0 0.0 11.428478980108558 0.0 108-109 0.0 0.0 0.0 11.807055435565154 0.0 110-111 0.0 0.0 0.0 12.27865815678566 0.0 112-113 0.0 0.0 0.0 12.760776890657738 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATAAC 15 2.6794072E-4 119.18395 3 GGTATCA 1810 0.0 102.47589 1 TATAACA 25 1.7064529E-5 95.34717 4 GCGGTAT 20 8.384677E-4 89.460464 1 CGGTATC 20 8.4117445E-4 89.38796 2 GTGGTAT 225 0.0 71.568375 1 GTATCAA 4100 0.0 65.167946 1 TGGTATC 265 0.0 60.716354 2 TGGGAGT 30 0.0042221034 59.519627 6 CTTAATC 35 0.007732508 51.07884 3 TATCAAC 5235 0.0 50.997528 2 GAGTGCA 35 0.0077821985 50.996185 9 ATCAACG 5385 0.0 49.35566 3 TCAACGC 5440 0.0 48.856655 4 CAACGCA 5555 0.0 48.00143 5 AACGCAG 5785 0.0 46.19587 6 ACGCAGA 6350 0.0 41.787422 7 CGCAGAG 6375 0.0 41.623554 8 GCAGAGT 6565 0.0 40.05641 9 TTAGGAA 30 0.0013070025 39.663696 46-47 >>END_MODULE