FastQCFastQC Report
Fri 27 May 2016
SRR940173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691711
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49850.061.01361
GGTATCA26900.056.8658831
TCAACGC62300.047.960824
ATCAACG62950.047.4655883
CAACGCA66000.045.2721065
TATCAAC67600.044.904972
AACGCAG67850.043.9369246
GTACATG141300.037.743031
ACGCAGA80700.036.6424377
CGCAGAG81750.035.955468
TACATGG148850.035.228812
ACATGGG149150.034.4396253
CATGGGG83950.029.565574
GCAGAGT99600.028.4363129
CATGGGA74750.028.347174
TATACCG1306.366013E-527.4712735
ATGGGGA53250.024.702655
GAGTACA108750.023.096921
AGAGTAC91450.022.9993310-11
AGTACAT106950.021.8717062
Produced by FastQC (version 0.11.5)