FastQCFastQC Report
Fri 27 May 2016
SRR940173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691711
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.17041205389434455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.13623305027917187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.072.782421
GTATCAA84300.062.1890341
ATCAACG110250.047.2679753
TCAACGC111200.046.969534
TATCAAC114250.045.6139222
CAACGCA114600.045.485985
AACGCAG118950.043.8717736
ACGCAGA137100.038.0560267
CGCAGAG139150.037.452628
GTACATG137450.037.101181
TACATGG145200.034.414462
ACATGGG144850.033.551293
GCAGAGT157700.032.783059
CATGGGG85800.029.4306564
GTGGTAT21750.027.6643731
TATTCCG4650.026.8735125
GAGTACT83750.026.46078712-13
CCGTTGC4500.026.4410559
AGAGTAC151250.026.25531210-11
CAGAGTA156250.025.94819510-11
Produced by FastQC (version 0.11.5)