##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4107 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.299245191137082 31.0 30.0 34.0 22.0 34.0 2 29.3654735816898 33.0 30.0 34.0 18.0 34.0 3 29.441684928171416 33.0 30.0 34.0 20.0 34.0 4 32.47041636230826 37.0 35.0 37.0 19.0 37.0 5 32.61602142683224 37.0 35.0 37.0 20.0 37.0 6 32.562941319698076 37.0 35.0 37.0 18.0 37.0 7 32.31799366934502 37.0 35.0 37.0 17.0 37.0 8 32.09885561236913 37.0 35.0 37.0 10.0 37.0 9 33.43973703433163 39.0 35.0 39.0 10.0 39.0 10-11 33.40345751156562 39.0 35.0 39.0 10.0 39.0 12-13 33.337959581202824 39.0 35.0 39.0 10.0 39.0 14-15 34.50791331872413 40.0 35.5 41.0 10.5 41.0 16-17 34.55429754078403 40.0 35.5 41.0 11.0 41.0 18-19 34.549306062819575 40.0 35.0 41.0 11.0 41.0 20-21 34.731555880204525 40.0 35.0 41.0 14.5 41.0 22-23 34.79681032383735 40.0 35.0 41.0 16.5 41.0 24-25 34.8345507669832 40.0 35.0 41.0 17.5 41.0 26-27 34.914292671049424 40.0 35.0 41.0 17.5 41.0 28-29 34.783905527148775 40.0 34.5 41.0 16.5 41.0 30-31 34.70075480886292 40.0 34.0 41.0 16.5 41.0 32-33 34.606281957633314 40.0 34.0 41.0 16.0 41.0 34-35 34.52994886778671 40.0 34.0 41.0 13.5 41.0 36-37 34.391404918431945 40.0 34.0 41.0 9.0 41.0 38-39 34.179814950085216 40.0 33.0 41.0 8.5 41.0 40-41 34.07584611638666 40.0 33.0 41.0 8.0 41.0 42-43 33.98928658388118 40.0 33.5 41.0 4.0 41.0 44-45 34.07389822254687 40.0 33.0 41.0 2.0 41.0 46-47 33.972242512783055 40.0 33.0 41.0 2.0 41.0 48-49 33.82444606768931 40.0 33.0 41.0 2.0 41.0 50-51 33.70477233990748 39.0 33.0 41.0 2.0 41.0 52-53 33.49257365473582 39.0 33.0 41.0 2.0 41.0 54-55 33.312880448015584 39.0 33.0 41.0 2.0 41.0 56-57 33.147796445093746 39.0 33.0 41.0 2.0 41.0 58-59 32.927927927927925 38.0 33.0 41.0 2.0 41.0 60-61 32.65120525931337 37.5 32.0 40.5 2.0 41.0 62-63 32.367056245434625 37.0 32.0 40.0 2.0 41.0 64-65 32.04309715120526 36.5 32.0 40.0 2.0 41.0 66-67 31.774531288044802 36.0 31.5 40.0 2.0 41.0 68-69 31.523983442902363 35.5 31.0 39.0 2.0 41.0 70-71 31.39992695398101 35.0 31.5 39.0 2.0 41.0 72-73 31.227903579254928 35.0 31.0 39.0 2.0 41.0 74-75 30.915510104699294 35.0 31.0 37.5 2.0 40.0 76-77 30.603968833698566 35.0 31.0 37.0 2.0 39.0 78-79 30.265765765765767 35.0 31.0 37.0 2.0 39.0 80-81 30.0018261504748 35.0 31.0 36.0 2.0 39.0 82-83 29.698563428293156 35.0 30.5 36.0 2.0 37.0 84-85 29.543218894570245 35.0 30.0 35.5 2.0 37.0 86-87 29.31653274896518 35.0 30.0 35.0 2.0 36.5 88-89 29.16143170197224 35.0 30.0 35.0 2.0 36.0 90-91 29.052349646944243 35.0 30.0 35.0 2.0 36.0 92-93 28.906135865595324 35.0 30.0 35.0 2.0 36.0 94-95 28.78865351838325 35.0 30.0 35.0 2.0 35.5 96-97 28.67336255174093 35.0 30.0 35.0 2.0 35.0 98-99 28.57755052349647 35.0 30.0 35.0 2.0 35.0 100-101 28.5445580715851 35.0 30.0 35.0 2.0 35.0 102-103 27.789870952033112 33.5 28.5 35.0 2.0 35.0 104-105 28.299488677867053 34.5 29.0 35.0 2.0 35.0 106-107 28.404553201850497 35.0 30.0 35.0 2.0 35.0 108-109 28.311419527635742 35.0 29.0 35.0 2.0 35.0 110-111 28.228390552714878 35.0 29.0 35.0 2.0 35.0 112-113 28.156318480642803 35.0 29.0 35.0 2.0 35.0 114-115 28.100803506208912 35.0 29.0 35.0 2.0 35.0 116-117 28.132578524470418 35.0 29.0 35.0 2.0 35.0 118-119 28.003165327489654 35.0 29.0 35.0 2.0 35.0 120-121 27.926953981008033 35.0 29.0 35.0 2.0 35.0 122-123 27.733260287314344 35.0 29.0 35.0 2.0 35.0 124-125 25.952763574385195 32.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.187903025793652 1101 2 1.012059771825399 1101 3 1.0167100694444393 1101 4 2.617286706349212 1101 5 2.6140500992063593 1101 6 1.2449838789682488 1101 7 1.7911272321428555 1101 8 3.301153273809515 1101 9 1.0758866567460217 1101 10-11 -1.489527529761908 1101 12-13 -0.1144779265873126 1101 14-15 2.669196428571432 1101 16-17 2.032558283730168 1101 18-19 2.589744543650795 1101 20-21 2.71586681547619 1101 22-23 -1.8940135168650727 1101 24-25 0.18981584821428044 1101 26-27 0.1584170386904873 1101 28-29 1.22671440972222 1101 30-31 2.7189887152777814 1101 32-33 -0.305484250992059 1101 34-35 1.3782335069444542 1101 36-37 -0.33682415674602595 1101 38-39 -0.30823722718254487 1101 40-41 -2.5782459077380935 1101 42-43 -2.4763950892857167 1101 44-45 -3.8506603422619037 1101 46-47 -4.050204613095232 1101 48-49 -4.966905381944457 1101 50-51 -5.921304563492058 1101 52-53 -6.661139012896829 1101 54-55 -3.5692584325396695 1101 56-57 -3.0890997023809383 1101 58-59 -1.518768601190473 1101 60-61 -2.4870597718254075 1101 62-63 -2.338864087301584 1101 64-65 -1.533221726190483 1101 66-67 -2.5584015376984226 1101 68-69 -2.3529637896825406 1101 70-71 -1.3706690228174594 1101 72-73 -3.2270740327380913 1101 74-75 -1.9967137896825413 1101 76-77 -3.1082961309523824 1101 78-79 -2.9350973462301617 1101 80-81 -1.6112754216269813 1101 82-83 -0.4387803819444507 1101 84-85 0.021999007936511106 1101 86-87 -0.27670200892857366 1101 88-89 -0.20544084821428044 1101 90-91 -1.9252945188492134 1101 92-93 -1.8609437003968203 1101 94-95 -0.7430772569444493 1101 96-97 0.36727430555555785 1101 98-99 -3.7282025049603114 1101 100-101 -1.9632440476190496 1101 102-103 -2.321161954365067 1101 104-105 -1.2778087797618944 1101 106-107 -0.3114552331349252 1101 108-109 -0.8282552083333314 1101 110-111 -0.538826884920649 1101 112-113 -0.4968812003968246 1101 114-115 -4.478927951388886 1101 116-117 -4.816648065476198 1101 118-119 -3.7215959821428584 1101 120-121 -3.80088975694445 1101 122-123 -4.071447172619045 1101 124-125 -4.644357638888891 1102 1 1.8545696924603163 1102 2 1.8453931051587311 1102 3 0.1833767361111036 1102 4 1.9506200396825406 1102 5 2.7807167658730236 1102 6 2.744983878968249 1102 7 2.2911272321428555 1102 8 3.4678199404761862 1102 9 3.742553323412693 1102 10-11 2.0938058035714278 1102 12-13 1.468855406746023 1102 14-15 2.5858630952381034 1102 16-17 3.532558283730161 1102 18-19 1.2564112103174594 1102 20-21 2.2992001488095255 1102 22-23 2.2726531498015916 1102 24-25 2.2731491815476232 1102 26-27 4.325083705357159 1102 28-29 1.5600477430555486 1102 30-31 3.052322048611117 1102 32-33 3.694515749007941 1102 34-35 3.961566840277783 1102 36-37 4.579842509920638 1102 38-39 3.941762772817455 1102 40-41 4.338420758928571 1102 42-43 4.273604910714283 1102 44-45 5.316006324404761 1102 46-47 3.9497953869047677 1102 48-49 3.6997612847222143 1102 50-51 2.2453621031746067 1102 52-53 0.4221943204365033 1102 54-55 0.43074156746033765 1102 56-57 -1.9224330357142705 1102 58-59 -1.6021019345238052 1102 60-61 0.6796068948412568 1102 62-63 1.2444692460317484 1102 64-65 0.13344494047618838 1102 66-67 -0.4750682043650869 1102 68-69 1.230369543650788 1102 70-71 0.5459976438492049 1102 72-73 1.1062593005952444 1102 74-75 1.0032862103174551 1102 76-77 1.0583705357142819 1102 78-79 0.398235987103174 1102 80-81 0.6387245783730187 1102 82-83 0.3112196180555493 1102 84-85 0.6886656746031754 1102 86-87 0.6399646577380906 1102 88-89 0.5445591517857196 1102 90-91 0.5747054811507866 1102 92-93 0.7223896329365083 1102 94-95 0.7569227430555507 1102 96-97 0.7006076388888864 1102 98-99 1.188464161706353 1102 100-101 -1.2965773809523853 1102 102-103 0.3455047123015973 1102 104-105 0.5555245535714342 1102 106-107 0.35521143353174267 1102 108-109 0.6717447916666686 1102 110-111 0.8778397817460153 1102 112-113 1.0031187996031754 1102 114-115 0.8544053819444457 1102 116-117 0.4333519345238024 1102 118-119 1.1950706845238095 1102 120-121 1.69911024305555 1102 122-123 1.845219494047619 1102 124-125 2.6889756944444443 1103 1 3.021236359126984 1103 2 3.212059771825402 1103 3 3.450043402777773 1103 4 4.383953373015878 1103 5 4.214050099206361 1103 6 4.178317212301586 1103 7 4.724460565476193 1103 8 4.734486607142852 1103 9 5.509219990079359 1103 10-11 5.293805803571431 1103 12-13 5.352188740079356 1103 14-15 5.119196428571435 1103 16-17 5.965891617063498 1103 18-19 5.823077876984122 1103 20-21 4.899200148809527 1103 22-23 5.67265314980159 1103 24-25 4.056482514880955 1103 26-27 5.4584170386904844 1103 28-29 5.860047743055546 1103 30-31 6.23565538194444 1103 32-33 5.577849082341267 1103 34-35 5.711566840277783 1103 36-37 5.579842509920638 1103 38-39 4.858429439484119 1103 40-41 6.888420758928568 1103 42-43 6.923604910714289 1103 44-45 5.866006324404758 1103 46-47 5.799795386904769 1103 48-49 5.58309461805554 1103 50-51 5.995362103174607 1103 52-53 4.9721943204365004 1103 54-55 6.364074900793668 1103 56-57 7.144233630952392 1103 58-59 4.6312313988095255 1103 60-61 6.146273561507925 1103 62-63 6.0444692460317455 1103 64-65 5.7501116071428555 1103 66-67 5.3749317956349145 1103 68-69 2.863702876984121 1103 70-71 5.429330977182538 1103 72-73 5.772925967261909 1103 74-75 6.186619543650785 1103 76-77 6.09170386904762 1103 78-79 5.731569320436506 1103 80-81 5.922057911706357 1103 82-83 5.8612196180555465 1103 84-85 4.471999007936514 1103 86-87 5.289964657738089 1103 88-89 5.061225818452385 1103 90-91 5.774705481150789 1103 92-93 4.7057229662698425 1103 94-95 5.906922743055549 1103 96-97 6.433940972222221 1103 98-99 5.338464161706359 1103 100-101 5.470089285714284 1103 102-103 5.595504712301597 1103 104-105 5.63885788690477 1103 106-107 6.0552114335317455 1103 108-109 6.455078125000007 1103 110-111 6.86117311507935 1103 112-113 6.98645213293651 1103 114-115 6.987738715277779 1103 116-117 6.4833519345237995 1103 118-119 7.045070684523811 1103 120-121 7.149110243055553 1103 122-123 7.3785528273809575 1103 124-125 2.005642361111107 1104 1 -1.312096974206348 1104 2 -0.8212735615079332 1104 3 -1.3166232638888964 1104 4 -1.3827132936507915 1104 5 -1.2192832341269728 1104 6 -1.2550161210317476 1104 7 -0.7088727678571409 1104 8 -0.6988467261904816 1104 9 -0.2574466765873069 1104 10-11 -0.15619419642857224 1104 12-13 -0.11447792658730549 1104 14-15 -0.4974702380952394 1104 16-17 -0.3841083829365033 1104 18-19 0.08974454365078799 1104 20-21 -0.1174665178571459 1104 22-23 -0.7273468501984084 1104 24-25 0.023149181547623243 1104 26-27 -1.1749162946428413 1104 28-29 -1.3566189236111157 1104 30-31 -1.0310112847222257 1104 32-33 -0.7221509176587304 1104 34-35 -0.9550998263888815 1104 36-37 -0.586824156746033 1104 38-39 -0.22490389384920917 1104 40-41 0.005087425595242223 1104 42-43 -0.05972842261904532 1104 44-45 -0.3506603422619108 1104 46-47 0.03312872023809632 1104 48-49 0.19976128472220722 1104 50-51 -0.2546378968253933 1104 52-53 0.005527653769831886 1104 54-55 0.09740823412699484 1104 56-57 0.6609002976190581 1104 58-59 0.4812313988095269 1104 60-61 0.3462735615079282 1104 62-63 0.5778025793650841 1104 64-65 -0.283221726190483 1104 66-67 0.691598462301581 1104 68-69 0.980369543650788 1104 70-71 1.1293309771825406 1104 72-73 0.7729259672619087 1104 74-75 1.0866195436507908 1104 76-77 0.9750372023809497 1104 78-79 0.5649026537698418 1104 80-81 0.8053912450396901 1104 82-83 0.644552951388885 1104 84-85 0.6886656746031754 1104 86-87 0.47329799107142634 1104 88-89 0.294559151785716 1104 90-91 0.49137214781745087 1104 92-93 0.805722966269844 1104 94-95 0.923589409722215 1104 96-97 1.20060763888889 1104 98-99 1.5217974950396886 1104 100-101 1.7034226190476147 1104 102-103 1.7621713789682651 1104 104-105 1.8888578869047699 1104 106-107 1.4385447668650784 1104 108-109 1.2550781250000043 1104 110-111 1.461173115079351 1104 112-113 1.753118799603179 1104 114-115 1.3544053819444493 1104 116-117 1.6833519345238024 1104 118-119 1.7784040178571452 1104 120-121 1.7824435763888857 1104 122-123 2.1785528273809547 1104 124-125 1.9389756944444443 1105 1 -4.693049355158731 1105 2 -4.130797371031743 1105 3 -4.864242311507944 1105 4 -5.216046626984124 1105 5 -5.385949900793641 1105 6 -5.4216827876984155 1105 7 -4.875539434523809 1105 8 -4.8655133928571495 1105 9 -4.662208581349216 1105 10-11 -4.477622767857145 1105 12-13 -4.590668402777787 1105 14-15 -4.366517857142853 1105 16-17 -4.848394097222219 1105 18-19 -4.719779265873019 1105 20-21 -4.7007998511904745 1105 22-23 -4.727346850198408 1105 24-25 -4.572088913690479 1105 26-27 -4.484440104166655 1105 28-29 -4.868523685515882 1105 30-31 -4.935773189484124 1105 32-33 -5.365008060515873 1105 34-35 -4.931290302579363 1105 36-37 -4.491586061507935 1105 38-39 -3.9987134176587347 1105 40-41 -4.6258649553571445 1105 42-43 -4.262109375000001 1105 44-45 -3.7911365327380935 1105 46-47 -4.15734747023809 1105 48-49 -4.145476810515888 1105 50-51 -3.861780753968251 1105 52-53 -3.399234250992066 1105 54-55 -3.093067956349188 1105 56-57 -2.898623511904752 1105 58-59 -2.768768601190473 1105 60-61 -2.9394407242063565 1105 62-63 -2.6841021825396822 1105 64-65 -2.4498883928571473 1105 66-67 -2.867925347222233 1105 68-69 -2.6505828373015916 1105 70-71 -2.942097594246029 1105 72-73 -3.2985026041666643 1105 74-75 -3.056237599206355 1105 76-77 -3.536867559523813 1105 78-79 -3.339859250992067 1105 80-81 -3.2779420882936456 1105 82-83 -3.51020895337302 1105 84-85 -3.228000992063489 1105 86-87 -3.1814639136904788 1105 88-89 -3.2530598958333314 1105 90-91 -3.2824373759920675 1105 92-93 -3.1228484623015866 1105 94-95 -2.9930772569444493 1105 96-97 -3.5374875992063473 1105 98-99 -3.2043929811507894 1105 100-101 -2.701339285714287 1105 102-103 -2.3330667162698333 1105 104-105 -2.2182849702380842 1105 106-107 -2.5733599950396844 1105 108-109 -2.8877790178571416 1105 110-111 -2.6102554563492184 1105 112-113 -2.4849764384920583 1105 114-115 -2.2408327132936456 1105 116-117 -2.2452194940476247 1105 118-119 -1.9120721726190446 1105 120-121 -1.8366040426587347 1105 122-123 -1.8214471726190453 1105 124-125 -2.680071924603176 1106 1 -1.5501922123015888 1106 2 -3.7022259424603163 1106 3 -3.007099454365086 1106 4 -1.5017609126984084 1106 5 -4.957378472222214 1106 6 -4.9931113591269884 1106 7 -1.8755394345238088 1106 8 -3.2940848214285765 1106 9 -4.9479228670635 1106 10-11 -0.8347656250000028 1106 12-13 -2.733525545634926 1106 14-15 -2.6522321428571374 1106 16-17 -0.9912512400793645 1106 18-19 -2.1483506944444493 1106 20-21 -2.91508556547619 1106 22-23 -3.3702039930555543 1106 24-25 -1.0720889136904788 1106 26-27 -3.2701543898809433 1106 28-29 -5.082809399801594 1106 30-31 -5.435773189484124 1106 32-33 -5.2935794890873 1106 34-35 -6.288433159722217 1106 36-37 -4.920157490079362 1106 38-39 -4.21299913194445 1106 40-41 -4.1258649553571445 1106 42-43 -3.9049665178571473 1106 44-45 -3.8625651041666664 1106 46-47 -4.014490327380948 1106 48-49 -4.716905381944457 1106 50-51 -4.004637896825393 1106 52-53 -4.613519965277781 1106 54-55 -4.164496527777761 1106 56-57 -3.898623511904752 1106 58-59 -4.697340029761904 1106 60-61 -4.296583581349214 1106 62-63 -4.041245039682536 1106 64-65 -4.878459821428578 1106 66-67 -4.296496775793656 1106 68-69 -4.864868551587307 1106 70-71 -4.156383308531744 1106 72-73 -4.012788318452376 1106 74-75 -3.984809027777782 1106 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64-65 4.925111607142853 2215 66-67 5.274931795634913 2215 68-69 4.438702876984124 2215 70-71 4.816830977182541 2215 72-73 5.397925967261909 2215 74-75 5.711619543650791 2215 76-77 4.141703869047618 2215 78-79 4.606569320436506 2215 80-81 4.409557911706354 2215 82-83 4.623719618055549 2215 84-85 5.084499007936511 2215 86-87 4.952464657738091 2215 88-89 4.711225818452384 2215 90-91 5.199705481150787 2215 92-93 5.368222966269844 2215 94-95 5.256922743055551 2215 96-97 5.096440972222222 2215 98-99 5.375964161706353 2215 100-101 5.557589285714283 2215 102-103 5.345504712301597 2215 104-105 5.38885788690477 2215 106-107 5.792711433531743 2215 108-109 5.630078125000004 2215 110-111 5.648673115079351 2215 112-113 5.336452132936511 2215 114-115 5.437738715277781 2215 116-117 5.183351934523802 2215 118-119 4.9450706845238095 2215 120-121 4.44911024305555 2215 122-123 6.053552827380955 2215 124-125 5.730642361111109 2216 1 1.5212363591269842 2216 2 1.8453931051587311 2216 3 0.8500434027777715 2216 4 -0.8827132936507915 2216 5 -3.219283234126973 2216 6 -1.0883494543650833 2216 7 -4.042206101190477 2216 8 -2.5321800595238173 2216 9 -4.2574466765873105 2216 10-11 -2.6561941964285722 2216 12-13 -1.1978112599206412 2216 14-15 -3.1641369047619037 2216 16-17 -2.1341083829365033 2216 18-19 -1.2435887896825477 2216 20-21 -1.1174665178571388 2216 22-23 0.10598648313492731 2216 24-25 2.023149181547616 2216 26-27 2.241750372023816 2216 28-29 2.1433810763888843 2216 30-31 2.2189887152777814 2216 32-33 2.444515749007934 2216 34-35 1.9615668402777828 2216 36-37 2.3298425099206383 2216 38-39 2.441762772817448 2216 40-41 1.8384207589285708 2216 42-43 3.6902715773809476 2216 44-45 2.3160063244047606 2216 46-47 1.3664620535714391 2216 48-49 1.533094618055543 2216 50-51 1.5786954365079424 2216 52-53 2.755527653769839 2216 54-55 3.6807415674603305 2216 56-57 4.077566964285722 2216 58-59 3.3145647321428626 2216 60-61 4.096273561507928 2216 62-63 3.4944692460317484 2216 64-65 0.6334449404761813 2216 66-67 0.6915984623015774 2216 68-69 2.3137028769841237 2216 70-71 3.1293309771825406 2216 72-73 3.5229259672619087 2216 74-75 1.2532862103174551 2216 76-77 -0.9416294642857146 2216 78-79 -1.0184306795634939 2216 80-81 -0.8612754216269813 2216 82-83 -0.6887803819444507 2216 84-85 -1.3113343253968246 2216 86-87 -0.9433686755952451 2216 88-89 -0.28877418154761614 2216 90-91 -0.5086278521825491 2216 92-93 0.13905629960317967 2216 94-95 -0.24307725694444926 2216 96-97 0.11727430555555429 2216 98-99 0.35513082837301724 2216 100-101 -0.29657738095238173 2216 102-103 -0.1544952876984027 2216 104-105 -0.36114211309523014 2216 106-107 -0.06145523313492163 2216 108-109 0.08841145833333997 2216 110-111 -1.1221602182539812 2216 112-113 -1.663547867063489 2216 114-115 -0.8122612847222186 2216 116-117 -0.8999813988095298 2216 118-119 0.6950706845238095 2216 120-121 0.6157769097222179 2216 122-123 0.34521949404762253 2216 124-125 1.1889756944444443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 263.0 3 39.0 4 17.0 5 13.0 6 17.0 7 19.0 8 18.0 9 18.0 10 26.0 11 27.0 12 22.0 13 22.0 14 16.0 15 23.0 16 14.0 17 14.0 18 15.0 19 13.0 20 10.0 21 12.0 22 13.0 23 18.0 24 33.0 25 33.0 26 61.0 27 46.0 28 44.0 29 69.0 30 73.0 31 66.0 32 96.0 33 139.0 34 180.0 35 286.0 36 552.0 37 1129.0 38 641.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.42951059167275 17.40930119308498 11.273435597759923 22.887752617482345 2 17.652787923058195 20.57462868273679 37.13172632091551 24.640857073289503 3 16.971025079133188 28.24446067689311 30.92281470659849 23.861699537375213 4 11.078646213781349 17.04407109812515 34.8672997321646 37.0099829559289 5 12.369125882639397 41.636230825420014 33.357682006330656 12.636961285609935 6 31.62892622352082 37.18042366691015 17.287557828098368 13.903092281470661 7 27.07572437302167 34.72120769418067 20.818115412710007 17.384952520087655 8 28.53664475286097 32.70026783540297 19.235451667884103 19.52763574385196 9 27.587046505965425 13.513513513513514 18.261504747991232 40.63793523252983 10-11 24.470416362308253 27.38008278548819 28.037496956415875 20.11200389578768 12-13 25.480886291697104 26.454833211589968 27.002678354029708 21.061602142683224 14-15 22.802532261991722 27.854881908935962 24.762600438276113 24.579985390796203 16-17 22.66861456050645 30.119308497686877 25.60262965668371 21.60944728512296 18-19 22.89992695398101 27.976625273922572 27.24616508400292 21.877282688093498 20-21 25.11565619673728 26.82006330654979 27.08789870952033 20.976381787192597 22-23 23.423423423423422 27.38008278548819 27.11224738251765 22.08424640857073 24-25 22.63209155101047 28.049671292914535 26.6617969320672 22.656440224007792 26-27 22.33990747504261 28.81665449233017 26.442658875091308 22.400779157535915 28-29 22.826880934989045 27.891404918431945 26.613099586072558 22.66861456050645 30-31 22.814706598490382 26.917457998539078 27.36790844898953 22.89992695398101 32-33 22.011200389578768 27.69661553445337 26.79571463355247 23.49646944241539 34-35 23.277331385439492 26.613099586072558 26.917457998539078 23.192111029948865 36-37 21.76771365960555 27.35573411249087 27.31921110299489 23.557341124908692 38-39 22.120769418066715 26.9418066715364 27.647918188458732 23.289505721938156 40-41 23.3382030679328 27.10007304601899 26.771365960555148 22.79035792549306 42-43 22.58339420501583 27.635743851960072 26.345264183102017 23.435597759922082 44-45 22.011200389578768 26.832237643048458 27.769661553445335 23.386900413927442 46-47 21.76771365960555 27.2218164110056 26.369612856099344 24.640857073289503 48-49 22.08424640857073 28.37837837837838 26.186997808619427 23.35037740443146 50-51 22.972972972972975 27.867056245434625 26.82006330654979 22.33990747504261 52-53 23.192111029948865 27.33138543949355 26.527879230581934 22.948624299975652 54-55 22.729486242999755 26.454833211589968 27.830533235938642 22.985147309471635 56-57 23.277331385439492 26.710494278061848 26.138300462624787 23.873873873873876 58-59 22.997321645970295 27.42878013148283 26.296566837107378 23.277331385439492 60-61 22.692963233503775 27.465303140978815 26.418310202093988 23.423423423423422 62-63 22.60774287801315 26.917457998539078 26.990504017531048 23.48429510591673 64-65 22.36425614803993 27.53834915997078 26.613099586072558 23.48429510591673 66-67 22.400779157535915 27.806184562941322 26.479181884587287 23.313854394935476 68-69 22.29121012904797 27.453128804480155 26.673971268565865 23.581689797906012 70-71 23.313854394935476 26.613099586072558 27.13659605551497 22.93644996347699 72-73 23.143413683954225 27.36790844898953 26.97832968103238 22.51034818602386 74-75 22.74166057949842 28.585342098855616 25.955685415144874 22.717311906501095 76-77 22.36425614803993 27.623569515461405 27.027027027027028 22.985147309471635 78-79 22.58339420501583 28.110542975407842 26.79571463355247 22.51034818602386 80-81 23.094716337959582 28.220112003895785 26.576576576576578 22.108595081568055 82-83 22.52252252252252 27.587046505965425 27.002678354029708 22.887752617482345 84-85 22.79035792549306 27.501826150474802 26.698319941563188 23.009495982468955 86-87 22.93644996347699 28.24446067689311 26.418310202093988 22.400779157535915 88-89 22.887752617482345 27.39225712198685 26.698319941563188 23.021670318967615 90-91 21.816411005600195 28.013148283418555 26.247869491112734 23.922571219868516 92-93 22.47382517652788 27.41660579498417 27.42878013148283 22.680788897005115 94-95 23.143413683954225 26.917457998539078 27.44095446798149 22.4981738495252 96-97 22.51034818602386 28.013148283418555 26.247869491112734 23.228634039444852 98-99 22.692963233503775 27.08789870952033 27.24616508400292 22.972972972972975 100-101 21.999026053080108 27.33138543949355 27.039201363525688 23.63038714390066 102-103 22.02337472607743 27.35573411249087 26.95398100803506 23.66691015339664 104-105 21.76771365960555 27.915753591429265 26.82006330654979 23.49646944241539 106-107 22.52252252252252 28.000973946919892 26.320915510104697 23.155588020452885 108-109 22.18164110056002 27.927927927927925 26.96615534453372 22.92427562697833 110-111 22.802532261991722 28.57316776235695 25.894813732651574 22.729486242999755 112-113 23.0581933284636 27.44095446798149 25.992208424640857 23.50864377891405 114-115 22.4981738495252 28.037496956415875 26.600925249573898 22.863403944485025 116-117 21.353786218651084 28.39055271487704 26.576576576576578 23.6790844898953 118-119 21.840759678597514 27.307036766496225 27.197467738008278 23.65473581689798 120-121 22.33990747504261 27.47747747747748 27.014852690528368 23.167762356951545 122-123 22.02337472607743 29.27927927927928 25.870465059654247 22.826880934989045 124-125 22.40623477837311 28.312226010716024 25.876765708718946 23.404773502191915 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.5 3 0.5 4 0.0 5 0.5 6 1.0 7 0.5 8 2.5 9 3.5 10 2.0 11 1.5 12 1.5 13 1.5 14 1.0 15 0.5 16 1.0 17 1.5 18 1.5 19 2.5 20 3.0 21 5.0 22 9.0 23 11.5 24 14.0 25 22.5 26 32.0 27 33.5 28 28.0 29 33.5 30 47.0 31 53.5 32 70.0 33 89.0 34 87.5 35 81.5 36 97.5 37 118.5 38 109.5 39 109.0 40 124.0 41 130.0 42 136.0 43 134.5 44 148.5 45 158.5 46 145.0 47 138.0 48 152.0 49 149.0 50 129.0 51 125.0 52 128.0 53 129.0 54 116.0 55 111.0 56 115.0 57 108.5 58 108.0 59 98.5 60 79.0 61 71.5 62 64.0 63 50.5 64 35.0 65 31.5 66 29.5 67 19.0 68 13.0 69 11.5 70 7.5 71 4.0 72 5.0 73 5.0 74 4.0 75 4.0 76 3.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 1.0 84 1.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.024348672997321647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4107.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.836376917458 #Duplication Level Percentage of deduplicated Percentage of total 1 96.1890243902439 92.18407596785974 2 3.3790650406504064 6.4767470172875585 3 0.35569105691056907 1.0226442658875092 4 0.05081300813008131 0.19478938397857318 5 0.025406504065040653 0.12174336498660823 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5 0.12174336498660823 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.012174336498660824 0.0 46-47 0.0 0.0 0.0 0.048697345994643294 0.0 48-49 0.0 0.0 0.0 0.060871682493304116 0.0 50-51 0.0 0.0 0.0 0.08522035549062576 0.0 52-53 0.0 0.0 0.0 0.12174336498660823 0.0 54-55 0.0 0.0 0.0 0.18261504747991236 0.0 56-57 0.0 0.0 0.0 0.19478938397857318 0.0 58-59 0.0 0.0 0.0 0.24348672997321646 0.0 60-61 0.0 0.0 0.0 0.34088142196250304 0.0 62-63 0.0 0.0 0.0 0.36523009495982467 0.0 64-65 0.0 0.0 0.0 0.4261017774531288 0.0 66-67 0.0 0.0 0.0 0.5234964694424153 0.0 68-69 0.0 0.0 0.0 0.5721938154370587 0.0 70-71 0.0 0.0 0.0 0.6574141709276844 0.0 72-73 0.0 0.0 0.0 0.8035062089116143 0.0 74-75 0.0 0.0 0.0 0.8400292184075968 0.0 76-77 0.0 0.0 0.0 0.9130752373995618 0.0 78-79 0.0 0.0 0.0 0.9861212563915267 0.0 80-81 0.0 0.0 0.0 1.0713416118821524 0.0 82-83 0.0 0.0 0.0 1.2539566593620646 0.0 84-85 0.0 0.0 0.0 1.460920379839299 0.0 86-87 0.0 0.0 0.0 1.5583150718285852 0.0 88-89 0.0 0.0 0.0 1.7165814463111762 0.0 90-91 0.0 0.0 0.0 1.8626734842951058 0.0 92-93 0.0 0.0 0.0 2.0209398587776963 0.0 94-95 0.0 0.0 0.0 2.2279035792549307 0.0 96-97 0.0 0.0 0.0 2.410518626734843 0.0 98-99 0.0 0.0 0.0 2.580959337716094 0.0 100-101 0.0 0.0 0.0 2.982712442171902 0.0 102-103 0.0 0.0 0.0 3.3601168736303872 0.0 104-105 0.0 0.0 0.0 3.664475286096908 0.0 106-107 0.0 0.0 0.0 4.005356708059411 0.0 108-109 0.0 0.0 0.0 4.370586803019235 0.0 110-111 0.0 0.0 0.0 4.711468224981738 0.0 112-113 0.0 0.0 0.0 5.015826637448259 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE