FastQCFastQC Report
Fri 27 May 2016
SRR940172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.9739469198928659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.48697345994643293No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG150.3652300949598247RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA120.2921840759678598RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG80.19478938397857318TruSeq Adapter, Index 1 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.17044071098125152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1460920379839299No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT50.12174336498660823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.12174336498660823No Hit
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA50.12174336498660823No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG50.12174336498660823TruSeq Adapter, Index 3 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152.5115226E-4119.02
GGTATCA152.5115226E-4119.01
TCAACGC207.8870857E-489.250015
TATCAAC207.8870857E-489.250013
CAACGCA207.8870857E-489.250016
AACGCAG250.001913293671.47
ACGCAGA250.001913293671.48
CGCAGAG250.001913293671.49
ATCAACG250.001913293671.44
Produced by FastQC (version 0.11.5)