##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940172_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4107 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41465790114439 34.0 31.0 34.0 31.0 34.0 2 32.50864377891405 34.0 31.0 34.0 30.0 34.0 3 32.530801071341614 34.0 31.0 34.0 30.0 34.0 4 36.046749452154856 37.0 35.0 37.0 35.0 37.0 5 35.99975651327003 37.0 35.0 37.0 35.0 37.0 6 36.05137570002435 37.0 36.0 37.0 35.0 37.0 7 36.033114195276355 37.0 35.0 37.0 35.0 37.0 8 36.03822741660579 37.0 36.0 37.0 35.0 37.0 9 37.847577307036765 39.0 38.0 39.0 35.0 39.0 10-11 37.782444606768934 39.0 38.0 39.0 35.0 39.0 12-13 37.760652544436326 39.0 38.0 39.0 35.0 39.0 14-15 39.030070611151686 41.0 38.5 41.0 35.5 41.0 16-17 39.03920136352569 41.0 38.0 41.0 36.0 41.0 18-19 39.14195276357438 41.0 39.0 41.0 35.0 41.0 20-21 39.13002191380569 41.0 39.0 41.0 35.0 41.0 22-23 39.0172875578281 41.0 39.0 41.0 35.0 41.0 24-25 39.02507913318725 41.0 39.0 41.0 35.0 41.0 26-27 38.777331385439496 41.0 38.0 41.0 34.5 41.0 28-29 38.502434867299726 40.5 38.0 41.0 34.0 41.0 30-31 38.326759191624056 40.0 38.0 41.0 34.0 41.0 32-33 38.06939371804236 40.0 37.0 41.0 33.5 41.0 34-35 37.77867056245435 40.0 37.0 41.0 33.0 41.0 36-37 37.584002921840764 40.0 36.5 41.0 33.0 41.0 38-39 37.36036036036036 40.0 36.0 41.0 32.0 41.0 40-41 37.11529096664232 40.0 36.0 41.0 32.0 41.0 42-43 36.865595325054784 40.0 35.5 41.0 31.0 41.0 44-45 36.72230338446555 40.0 35.5 41.0 31.0 41.0 46-47 36.2100073046019 40.0 36.0 41.0 29.0 41.0 48-49 35.82493304114926 40.0 35.0 41.0 26.5 41.0 50-51 35.46712929145362 40.0 35.0 41.0 24.0 41.0 52-53 35.30715850986121 40.0 35.0 41.0 24.0 41.0 54-55 35.10421232042853 39.0 35.0 41.0 23.5 41.0 56-57 34.820428536644755 39.0 35.0 41.0 23.0 41.0 58-59 34.41149257365474 39.0 35.0 41.0 19.5 41.0 60-61 33.89688336985634 38.0 34.0 41.0 14.0 41.0 62-63 33.65351838324811 38.0 34.0 41.0 10.0 41.0 64-65 33.31373265157049 37.0 34.0 40.0 10.0 41.0 66-67 33.043218894570245 37.0 34.0 40.0 9.0 41.0 68-69 32.77562697832968 36.0 33.0 39.5 8.0 41.0 70-71 32.61918675432189 36.0 33.5 39.0 7.0 41.0 72-73 32.436571706841974 35.0 34.0 39.0 7.0 41.0 74-75 32.10068176284393 35.0 33.0 37.5 6.5 40.5 76-77 31.81166301436572 35.0 33.0 37.0 2.0 39.0 78-79 31.544923301680058 35.0 33.0 37.0 2.0 39.0 80-81 31.234477720964207 35.0 33.0 36.0 2.0 38.5 82-83 30.971268565863163 35.0 33.0 36.0 2.0 37.0 84-85 30.827854881908934 35.0 33.0 35.5 2.0 37.0 86-87 30.623326028731434 35.0 33.0 35.0 2.0 36.5 88-89 30.475529583637694 35.0 33.0 35.0 2.0 36.0 90-91 30.34794253713173 35.0 32.5 35.0 2.0 36.0 92-93 30.255782809836866 35.0 33.0 35.0 2.0 36.0 94-95 30.154248843438033 35.0 32.5 35.0 2.0 35.5 96-97 30.01643535427319 35.0 32.0 35.0 2.0 35.0 98-99 29.93206720233747 35.0 32.0 35.0 2.0 35.0 100-101 29.873386900413927 35.0 32.0 35.0 2.0 35.0 102-103 29.026296566837107 34.0 30.0 35.0 2.0 35.0 104-105 29.650596542488437 35.0 32.0 35.0 2.0 35.0 106-107 29.777209642074506 35.0 32.0 35.0 2.0 35.0 108-109 29.81336742147553 35.0 32.0 35.0 2.0 35.0 110-111 29.764426588750915 35.0 32.0 35.0 2.0 35.0 112-113 29.679084489895303 35.0 32.0 35.0 2.0 35.0 114-115 29.623691258826394 35.0 32.0 35.0 2.0 35.0 116-117 29.538227416605796 35.0 32.0 35.0 2.0 35.0 118-119 29.52130508887266 35.0 32.0 35.0 2.0 35.0 120-121 29.35463842220599 35.0 32.0 35.0 2.0 35.0 122-123 29.17190163136109 35.0 32.0 35.0 2.0 35.0 124-125 27.423301680058437 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8503534226190368 1101 2 -1.7987847222222193 1101 3 -2.348177083333333 1101 4 -1.0534164186507908 1101 5 -0.3790240575396737 1101 6 0.005809771825390442 1101 7 -1.4049665178571402 1101 8 -0.44387400793652176 1101 9 0.4418030753968125 1101 10-11 -0.05756758432537623 1101 12-13 -0.46088169642856514 1101 14-15 -0.6425099206349216 1101 16-17 -0.9393353174603121 1101 18-19 -2.7990017361111015 1101 20-21 -1.941827876984128 1101 22-23 -3.083547247023816 1101 24-25 -3.654154265873011 1101 26-27 -1.1871000744047606 1101 28-29 -2.457006448412706 1101 30-31 -2.151466393849226 1101 32-33 -2.2868210565476303 1101 34-35 -0.06934523809523085 1101 36-37 1.0342571924603305 1101 38-39 0.02147507440476204 1101 40-41 0.26202876984127244 1101 42-43 -1.28407118055555 1101 44-45 0.17713913690476346 1101 46-47 1.3392299107142804 1101 48-49 1.246558779761898 1101 50-51 1.5496155753968281 1101 52-53 2.3610119047619165 1101 54-55 2.4434926835317583 1101 56-57 -3.6161861359126917 1101 58-59 -0.30384734623014964 1101 60-61 1.0756448412698418 1101 62-63 1.7704551091269778 1101 64-65 1.2789093501984041 1101 66-67 1.5203807043650883 1101 68-69 3.149798487103169 1101 70-71 2.5830729166666657 1101 72-73 0.6465463789682495 1101 74-75 2.334133184523811 1101 76-77 2.802219742063496 1101 78-79 1.6257037450396794 1101 80-81 1.9493055555555578 1101 82-83 1.06364087301586 1101 84-85 3.3559244791666565 1101 86-87 -0.37458147321428825 1101 88-89 3.5836061507936527 1101 90-91 2.411142113095231 1101 92-93 2.4002852182539804 1101 94-95 1.1770616319444471 1101 96-97 -0.3391679067460345 1101 98-99 0.9121341765873012 1101 100-101 -2.5957124255952344 1101 102-103 -1.3431020585317448 1101 104-105 1.573301091269844 1101 106-107 -2.2994450644841145 1101 108-109 -1.4775266617063423 1101 110-111 -1.7458457341269877 1101 112-113 -0.5474361359126938 1101 114-115 -2.4648623511904795 1101 116-117 -2.0447203621031846 1101 118-119 -8.01329365079365 1101 120-121 -6.426934523809521 1101 122-123 -6.313120039682545 1101 124-125 -4.129160466269841 1102 1 0.6496465773809632 1102 2 -0.29878472222222285 1102 3 -0.18151041666666856 1102 4 0.44658358134920917 1102 5 -0.8790240575396737 1102 6 -0.49419022817460956 1102 7 -0.5716331845238045 1102 8 -0.27720734126985036 1102 9 -0.05819692460318748 1102 10-11 -0.9742342509920405 1102 12-13 -0.21088169642856514 1102 14-15 0.27415674603174267 1102 16-17 -1.3560019841269835 1102 18-19 -0.4656684027777658 1102 20-21 -5.358494543650785 1102 22-23 -5.416880580357152 1102 24-25 -6.070820932539668 1102 26-27 -5.353766741071432 1102 28-29 -4.873673115079377 1102 30-31 -4.984799727182569 1102 32-33 -4.370154389880966 1102 34-35 -4.319345238095231 1102 36-37 -3.9657428075396695 1102 38-39 -4.728524925595238 1102 40-41 -4.904637896825395 1102 42-43 -5.200737847222214 1102 44-45 -4.489527529761908 1102 46-47 -4.41077008928572 1102 48-49 -2.920107886904759 1102 50-51 -2.7003844246031647 1102 52-53 -2.555654761904748 1102 54-55 -1.973173983134906 1102 56-57 -1.3661861359126846 1102 58-59 -2.637180679563489 1102 60-61 -0.7576884920634939 1102 62-63 -0.8962115575396865 1102 64-65 -0.9710906498015959 1102 66-67 -0.9796192956349081 1102 68-69 -0.9335348462301631 1102 70-71 -1.5835937500000021 1102 72-73 -1.1867869543650862 1102 74-75 -1.332533482142857 1102 76-77 -0.8644469246031754 1102 78-79 -1.2076295882936527 1102 80-81 -0.6340277777777779 1102 82-83 -1.1030257936508079 1102 84-85 -0.6440755208333435 1102 86-87 -0.29124813988095255 1102 88-89 -0.33306051587301155 1102 90-91 -0.25552455357143344 1102 92-93 -1.1830481150793553 1102 94-95 -0.9896050347222172 1102 96-97 -0.5891679067460345 1102 98-99 -0.3378658234126988 1102 100-101 0.07095424107143344 1102 102-103 -0.1764353918650805 1102 104-105 -0.676698908730156 1102 106-107 -1.049445064484111 1102 108-109 -1.060859995039678 1102 110-111 -0.1625124007936556 1102 112-113 0.36923053075397405 1102 114-115 0.2851376488095205 1102 116-117 0.5386129712301511 1102 118-119 0.5700396825396865 1102 120-121 0.15639880952381446 1102 122-123 0.7702132936507908 1102 124-125 1.7875062003968232 1103 1 -2.1503534226190375 1103 2 -0.2654513888888843 1103 3 -0.7481770833333314 1103 4 -2.486749751984128 1103 5 -0.8456907242063352 1103 6 0.005809771825390442 1103 7 0.5283668154761969 1103 8 0.48945932539681536 1103 9 0.8751364087301496 1103 10-11 0.9924324156746209 1103 12-13 0.3891183035714292 1103 14-15 0.5241567460317427 1103 16-17 -0.10600198412698347 1103 18-19 -0.33233506944444 1103 20-21 -0.9751612103174594 1103 22-23 0.21645275297618127 1103 24-25 1.1125124007936549 1103 26-27 1.646233258928568 1103 28-29 0.22632688492062414 1103 30-31 0.08186693948410806 1103 32-33 0.3465122767856954 1103 34-35 0.630654761904772 1103 36-37 0.634257192460332 1103 38-39 3.271475074404762 1103 40-41 2.862028769841274 1103 42-43 0.26592881944444713 1103 44-45 3.060472470238089 1103 46-47 2.0892299107142804 1103 48-49 4.063225446428568 1103 50-51 3.4829489087301653 1103 52-53 4.261011904761915 1103 54-55 3.593492683531764 1103 56-57 3.3671471974206426 1103 58-59 1.279485987103179 1103 60-61 -0.12435515873016101 1103 62-63 5.120455109126979 1103 64-65 -1.5210906498015966 1103 66-67 -1.5462859623015746 1103 68-69 -1.350201512896831 1103 70-71 -1.3669270833333336 1103 72-73 -1.0534536210317498 1103 74-75 -1.3992001488095234 1103 76-77 -3.264446924603174 1103 78-79 -1.4242962549603178 1103 80-81 -2.4006944444444436 1103 82-83 -1.2363591269841407 1103 84-85 -1.2107421875000064 1103 86-87 -1.4912481398809518 1103 88-89 -1.4663938492063444 1103 90-91 -1.8055245535714342 1103 92-93 -1.449714781746021 1103 94-95 -1.2729383680555522 1103 96-97 -1.0058345734126988 1103 98-99 -1.2878658234127016 1103 100-101 -0.8290457589285651 1103 102-103 -0.6931020585317462 1103 104-105 -0.9766989087301567 1103 106-107 -0.732778397817448 1103 108-109 -0.27752666170633944 1103 110-111 -0.7791790674603263 1103 112-113 -0.7641028025793624 1103 114-115 -0.6315290178571438 1103 116-117 -0.5613870287698504 1103 118-119 -2.013293650793649 1103 120-121 -1.3936011904761827 1103 122-123 -0.1631200396825463 1103 124-125 0.4708395337301603 1104 1 0.9829799107142989 1104 2 1.0345486111111128 1104 3 1.1518229166666671 1104 4 1.1132502480158735 1104 5 0.6209759424603263 1104 6 0.8391431051587261 1104 7 0.9283668154761955 1104 8 0.8894593253968139 1104 9 0.2751364087301482 1104 10-11 0.6924324156746238 1104 12-13 0.9557849702381063 1104 14-15 1.5241567460317427 1104 16-17 1.4773313492063451 1104 18-19 1.0343315972222271 1104 20-21 0.9748387896825434 1104 22-23 0.8331194196428484 1104 24-25 1.5125124007936606 1104 26-27 1.896233258928568 1104 28-29 1.042993551587287 1104 30-31 2.098533606150774 1104 32-33 2.5465122767856982 1104 34-35 3.0973214285714405 1104 36-37 3.1175905257936662 1104 38-39 3.021475074404762 1104 40-41 3.7620287698412724 1104 42-43 3.6325954861111214 1104 44-45 4.343805803571421 1104 46-47 4.505896577380952 1104 48-49 4.329892113095241 1104 50-51 4.882948908730164 1104 52-53 4.944345238095252 1104 54-55 5.443492683531758 1104 56-57 5.133813864087315 1104 58-59 4.946152653769843 1104 60-61 4.492311507936513 1104 62-63 4.353788442460306 1104 64-65 3.8622426835317327 1104 66-67 2.853714037698424 1104 68-69 3.4831318204364976 1104 70-71 3.9164062499999943 1104 72-73 4.313213045634914 1104 74-75 4.417466517857147 1104 76-77 4.218886408730153 1104 78-79 4.292370411706351 1104 80-81 4.199305555555558 1104 82-83 4.396974206349196 1104 84-85 4.855924479166664 1104 86-87 4.792085193452376 1104 88-89 5.000272817460324 1104 90-91 5.244475446428567 1104 92-93 5.4002852182539804 1104 94-95 5.5937282986111185 1104 96-97 5.327498759920637 1104 98-99 5.328800843253973 1104 100-101 5.737620907738098 1104 102-103 6.3235646081349195 1104 104-105 6.156634424603173 1104 106-107 5.950554935515882 1104 108-109 6.022473338293658 1104 110-111 4.754154265873005 1104 112-113 5.285897197420638 1104 114-115 5.785137648809528 1104 116-117 6.038612971230151 1104 118-119 5.486706349206351 1104 120-121 6.073065476190479 1104 122-123 6.270213293650784 1104 124-125 7.120839533730159 1105 1 -0.49321056547617914 1105 2 0.10597718253968225 1105 3 0.36610863095238244 1105 4 -1.0296068948412724 1105 5 -0.18854786706348392 1105 6 -0.13704737103175546 1105 7 0.07122395833334139 1105 8 -0.25339781746033196 1105 9 -1.7248635912698518 1105 10-11 -0.5218532986110915 1105 12-13 -0.9251674107142804 1105 14-15 -1.6901289682539726 1105 16-17 -1.6060019841269835 1105 18-19 -1.4894779265872984 1105 20-21 -1.0608754960317412 1105 22-23 -0.2978329613095312 1105 24-25 -0.05891617063490884 1105 26-27 -0.5680524553571473 1105 28-29 0.2691840277777686 1105 30-31 0.4675812251983942 1105 32-33 -1.16777343750001 1105 34-35 0.21636904761905384 1105 36-37 0.31997147817461524 1105 38-39 0.05718936011904674 1105 40-41 0.26202876984127244 1105 42-43 -1.1054997519841194 1105 44-45 -4.382384672619054 1105 46-47 -0.982198660714289 1105 48-49 0.6632254464285694 1105 50-51 1.8115203373015945 1105 52-53 -0.28184523809522233 1105 54-55 -3.663650173611096 1105 56-57 -2.3185670882936407 1105 58-59 -2.1490854414682516 1105 60-61 -3.0672123015873005 1105 62-63 -4.765259176587307 1105 64-65 -4.221090649801596 1105 66-67 -2.7177145337301454 1105 68-69 -2.2073443700396886 1105 70-71 -1.3097842261904766 1105 72-73 -1.9963107638888928 1105 74-75 -4.71348586309524 1105 76-77 -2.9358754960317484 1105 78-79 -1.9100105406746053 1105 80-81 -1.0864087301587269 1105 82-83 -0.7220734126984247 1105 84-85 -1.5964564732142925 1105 86-87 -1.2912481398809526 1105 88-89 -2.7378224206349167 1105 90-91 -1.1483816964285758 1105 92-93 -1.992571924603162 1105 94-95 -0.07293836805555287 1105 96-97 0.3513082837301553 1105 98-99 -1.4450086805555529 1105 100-101 -0.35761718749999716 1105 102-103 -0.23595920138888715 1105 104-105 -1.8909846230158713 1105 106-107 -0.27563554067458895 1105 108-109 -2.3346695188491964 1105 110-111 -0.507750496031754 1105 112-113 -0.24981708829364635 1105 114-115 0.08275669642857153 1105 116-117 -1.461387028769849 1105 118-119 -4.299007936507934 1105 120-121 -4.37931547619047 1105 122-123 -4.77740575396826 1105 124-125 -3.557731894841268 1106 1 1.0782180059523938 1106 2 0.9631200396825434 1106 3 1.2232514880952365 1106 4 0.8275359623015888 1106 5 0.954309275793662 1106 6 0.7200954861111057 1106 7 0.9283668154761955 1106 8 0.8894593253968139 1106 9 1.2751364087301482 1106 10-11 0.8352895585317626 1106 12-13 0.5034040178571502 1106 14-15 0.8098710317460345 1106 16-17 1.1797123015873083 1106 18-19 1.3676649305555628 1106 20-21 1.2962673611111128 1106 22-23 0.3450241815476147 1106 24-25 1.4410838293650912 1106 26-27 1.5748046874999986 1106 28-29 1.6263268849206227 1106 30-31 1.5390097966269636 1106 32-33 0.832226562499983 1106 34-35 1.1449404761904844 1106 36-37 1.5342571924603305 1106 38-39 1.9857607886904773 1106 40-41 2.4763144841269877 1106 42-43 2.5373573908730194 1106 44-45 1.0461867559523768 1106 46-47 3.017801339285711 1106 48-49 2.091796875 1106 50-51 3.597234623015879 1106 52-53 3.7181547619047706 1106 54-55 4.193492683531758 1106 56-57 3.967147197420644 1106 58-59 4.422343129960318 1106 60-61 5.218501984126981 1106 62-63 4.877597966269839 1106 64-65 4.921766493055543 1106 66-67 5.067999751984132 1106 68-69 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-5.9299603174603135 2216 120-121 -5.8436011904761855 2216 122-123 -5.979786706349209 2216 124-125 -6.212493799603173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 2.0 6 4.0 7 6.0 8 8.0 9 12.0 10 19.0 11 20.0 12 29.0 13 44.0 14 82.0 15 65.0 16 30.0 17 36.0 18 27.0 19 21.0 20 20.0 21 23.0 22 14.0 23 13.0 24 22.0 25 23.0 26 30.0 27 36.0 28 52.0 29 61.0 30 65.0 31 79.0 32 101.0 33 129.0 34 188.0 35 267.0 36 630.0 37 1226.0 38 718.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.99293888483078 18.821524226929633 14.243973703433163 19.941563184806427 2 19.38154370586803 20.964207450693937 34.74555636717799 24.908692476260043 3 16.167518870221574 26.199172145118094 35.15948380813246 22.47382517652788 4 10.572472594397077 18.197320341047504 34.42143727161998 36.80876979293544 5 11.930849768687606 40.66228390552715 34.01509617725834 13.391770148526906 6 30.168005843681517 38.13002191380569 16.313610908205504 15.38836133430728 7 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33.75943511078646 21.232042853664478 30-31 20.759771094606112 24.461220017046145 33.44697430902228 21.33203457932546 32-33 20.927684441197954 24.653031409788166 32.91940589237887 21.499878256635014 34-35 21.0372534696859 25.334794253713174 32.736790844898955 20.89116143170197 36-37 21.292914536157777 23.52081811541271 32.408083759435115 22.7781835889944 38-39 22.05989773557341 24.287801314828343 31.95763330898466 21.694667640613584 40-41 20.952033114195277 24.482590698806916 32.88288288288289 21.682493304114924 42-43 20.635500365230094 24.12953494034575 33.1872412953494 22.04772339907475 44-45 22.61991721451181 24.87216946676406 31.20282444606769 21.30508887265644 46-47 22.63484719347376 25.410933885303788 29.575063923048827 22.37915499817363 48-49 22.30338446554663 25.846116386656924 30.143657170684197 21.706841977112248 50-51 21.916473882868623 26.34847193473761 28.91756970656277 22.817484475830998 52-53 21.950328707085465 28.013148283418555 27.44095446798149 22.59556854151449 54-55 21.694667640613584 28.4027270513757 27.794010226442662 22.108595081568055 56-57 23.155588020452885 28.183588994399805 27.660092524957392 21.00073046018992 58-59 21.816411005600195 28.87752617482347 27.38008278548819 21.92598003408814 60-61 21.451180910640367 28.293158022887756 27.976625273922572 22.27903579254931 62-63 21.731190650109568 27.514000486973462 29.072315558802046 21.682493304114924 64-65 23.094716337959582 25.943511078646214 29.23058193328464 21.731190650109568 66-67 22.53469685902118 26.722668614560508 28.81665449233017 21.92598003408814 68-69 22.400779157535915 26.893109325541758 27.915753591429265 22.79035792549306 70-71 21.792062332602875 27.11224738251765 29.0236182128074 22.07207207207207 72-73 22.37643048453859 27.014852690528368 27.635743851960072 22.972972972972975 74-75 22.74166057949842 26.588750913075238 28.049671292914535 22.61991721451181 76-77 22.66861456050645 27.35573411249087 27.41660579498417 22.559045532018505 78-79 21.938154370586805 27.465303140978815 27.25833942050158 23.3382030679328 80-81 22.218164110056 27.976625273922572 27.185293401509618 22.61991721451181 82-83 22.61991721451181 26.588750913075238 28.25663501339177 22.53469685902118 84-85 22.327733138543948 27.1609447285123 27.757487216946675 22.75383491599708 86-87 22.05989773557341 26.637448259069878 28.195763330898465 23.106890674458242 88-89 22.972972972972975 26.345264183102017 28.013148283418555 22.66861456050645 90-91 21.816411005600195 27.52617482347212 27.976625273922572 22.680788897005115 92-93 21.877282688093498 27.757487216946675 27.988799610421232 22.37643048453859 94-95 22.42512783053324 26.065254443632824 28.013148283418555 23.49646944241539 96-97 23.0581933284636 26.211346481616754 27.781835889943995 22.948624299975652 98-99 21.852934015096174 26.357438519600684 28.451424397370346 23.3382030679328 100-101 21.572924275626978 26.065254443632824 29.16971025079133 23.192111029948865 102-103 22.35208181154127 26.868760652544438 27.891404918431945 22.887752617482345 104-105 22.327733138543948 25.980034088142194 27.623569515461405 24.068663257852446 106-107 21.536401266130994 26.96615534453372 27.36790844898953 24.12953494034575 108-109 22.19381543705868 27.002678354029708 27.757487216946675 23.046018991964935 110-111 22.60774287801315 26.369612856099344 27.40443145848551 23.618212807402 112-113 22.187043351193374 26.656113005358012 27.36239649293716 23.79444715051145 114-115 21.402483564645728 26.96615534453372 27.514000486973462 24.11736060384709 116-117 22.315558802045288 27.453128804480155 27.11224738251765 23.119065010956902 118-119 21.792062332602875 27.52617482347212 26.637448259069878 24.044314584855126 120-121 21.597272948624298 27.757487216946675 27.2218164110056 23.423423423423422 122-123 21.268565863160457 26.80788897005113 27.940102264426585 23.98344290236182 124-125 22.384606016319573 26.866398733406406 26.610644257703083 24.138350992570942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 2.0 2 1.5 3 0.5 4 0.5 5 0.5 6 2.0 7 7.0 8 6.0 9 8.0 10 10.5 11 4.5 12 3.0 13 7.5 14 9.0 15 7.5 16 8.0 17 8.0 18 8.0 19 12.0 20 18.5 21 19.0 22 19.0 23 21.0 24 22.0 25 19.5 26 25.5 27 36.5 28 42.5 29 51.0 30 57.0 31 61.0 32 76.5 33 84.5 34 85.0 35 95.5 36 87.0 37 92.5 38 119.5 39 116.5 40 102.5 41 113.5 42 117.0 43 120.5 44 140.5 45 151.0 46 147.0 47 139.5 48 138.5 49 134.0 50 135.0 51 123.5 52 123.5 53 129.5 54 121.0 55 125.0 56 118.0 57 108.5 58 104.0 59 86.5 60 69.0 61 69.0 62 60.0 63 39.5 64 30.0 65 25.0 66 19.0 67 14.5 68 11.5 69 9.0 70 6.5 71 4.0 72 4.5 73 4.0 74 3.0 75 2.0 76 0.5 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.048697345994643294 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.012174336498660824 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.012174336498660824 48-49 0.0 50-51 0.012174336498660824 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.024348672997321647 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.03652300949598247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4107.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.86754321889457 #Duplication Level Percentage of deduplicated Percentage of total 1 94.8051948051948 87.09520331141952 2 4.214153193745031 7.742878013148284 3 0.5300821627352239 1.460920379839299 4 0.1590246488205672 0.5843681519357196 5 0.10601643254704478 0.48697345994643293 6 0.026504108136761195 0.1460920379839299 7 0.026504108136761195 0.17044071098125152 8 0.026504108136761195 0.19478938397857318 9 0.0 0.0 >10 0.10601643254704478 2.118334550766983 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.9739469198928659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.48697345994643293 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 15 0.3652300949598247 RNA PCR Primer, Index 26 (100% over 29bp) CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA 12 0.2921840759678598 RNA PCR Primer, Index 26 (100% over 29bp) TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 8 0.19478938397857318 TruSeq Adapter, Index 1 (96% over 28bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17044071098125152 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.1460920379839299 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 5 0.12174336498660823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 5 0.12174336498660823 No Hit GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA 5 0.12174336498660823 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 5 0.12174336498660823 TruSeq Adapter, Index 3 (96% over 28bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.012174336498660824 0.0 46-47 0.0 0.0 0.0 0.048697345994643294 0.0 48-49 0.0 0.0 0.0 0.060871682493304116 0.0 50-51 0.0 0.0 0.0 0.08522035549062576 0.0 52-53 0.0 0.0 0.0 0.12174336498660823 0.0 54-55 0.0 0.0 0.0 0.15826637448259068 0.0 56-57 0.0 0.0 0.0 0.17044071098125152 0.0 58-59 0.0 0.0 0.0 0.21913805697589483 0.0 60-61 0.0 0.0 0.0 0.3165327489651814 0.0 62-63 0.0 0.0 0.0 0.34088142196250304 0.0 64-65 0.0 0.0 0.0 0.40175310445580714 0.0 66-67 0.0 0.0 0.0 0.49914779644509377 0.0 68-69 0.0 0.0 0.0 0.547845142439737 0.0 70-71 0.0 0.0 0.0 0.6330654979303627 0.0 72-73 0.0 0.0 0.0 0.7791575359142927 0.0 74-75 0.0 0.0 0.0 0.8156805454102751 0.0 76-77 0.0 0.0 0.0 0.8765522279035792 0.0 78-79 0.0 0.0 0.0 0.9374239103968833 0.0 80-81 0.0 0.0 0.0 0.9982955928901875 0.0 82-83 0.0 0.0 0.0 1.1809106403701 0.0 84-85 0.0 0.0 0.0 1.3878743608473338 0.0 86-87 0.0 0.0 0.0 1.4730947163379597 0.0 88-89 0.0 0.0 0.0 1.6070124178232286 0.0 90-91 0.0 0.0 0.0 1.7652787923058195 0.0 92-93 0.0 0.0 0.0 1.9478938397857317 0.0 94-95 0.0 0.0 0.0 2.1548575602629656 0.0 96-97 0.0 0.0 0.0 2.3374726077428782 0.0 98-99 0.0 0.0 0.0 2.544436328220112 0.0 100-101 0.0 0.0 0.0 2.909666423179937 0.0 102-103 0.0 0.0 0.0 3.287070854638422 0.0 104-105 0.0 0.0 0.0 3.567080594107621 0.0 106-107 0.0 0.0 0.0 3.9079620160701243 0.0 108-109 0.0 0.0 0.0 4.29754078402727 0.0 110-111 0.0 0.0 0.0 4.638422205989773 0.0 112-113 0.0 0.0 0.0 4.906257608960312 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15 2.5115226E-4 119.0 2 GGTATCA 15 2.5115226E-4 119.0 1 TCAACGC 20 7.8870857E-4 89.25001 5 TATCAAC 20 7.8870857E-4 89.25001 3 CAACGCA 20 7.8870857E-4 89.25001 6 AACGCAG 25 0.0019132936 71.4 7 ACGCAGA 25 0.0019132936 71.4 8 CGCAGAG 25 0.0019132936 71.4 9 ATCAACG 25 0.0019132936 71.4 4 >>END_MODULE