##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 183524 Sequences flagged as poor quality 0 Sequence length 125 %GC 29 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.963972014559403 31.0 20.0 34.0 7.0 34.0 2 26.48310302739696 31.0 23.0 34.0 7.0 34.0 3 27.2435757721061 31.0 25.0 34.0 7.0 34.0 4 28.968385606242236 37.0 23.0 37.0 7.0 37.0 5 30.93780649942242 37.0 31.0 37.0 12.0 37.0 6 29.442132909047317 37.0 25.0 37.0 8.0 37.0 7 29.962310106580066 37.0 31.0 37.0 8.0 37.0 8 29.26902203526514 37.0 25.0 37.0 8.0 37.0 9 29.93426472831891 39.0 19.0 39.0 7.0 39.0 10-11 30.78157897604673 39.0 28.5 39.0 7.5 39.0 12-13 30.820094374577714 39.0 28.5 39.0 7.0 39.0 14-15 31.9118425927944 40.0 29.0 41.0 7.0 41.0 16-17 32.29061866567861 39.5 30.0 41.0 7.0 41.0 18-19 32.13413504500774 39.0 30.0 41.0 4.5 41.0 20-21 31.990063969835006 39.0 30.0 41.0 2.0 41.0 22-23 31.87247444475927 39.0 29.0 41.0 2.0 41.0 24-25 31.76333885486367 39.0 28.0 41.0 2.0 41.0 26-27 31.56001122468996 39.0 27.5 41.0 2.0 41.0 28-29 31.29305976330071 39.0 27.0 41.0 2.0 41.0 30-31 31.012668642793315 38.0 25.0 41.0 2.0 41.0 32-33 30.783216364072274 38.0 25.0 41.0 2.0 41.0 34-35 30.544326627580045 38.0 25.0 41.0 2.0 41.0 36-37 30.26215099932434 38.0 25.0 41.0 2.0 41.0 38-39 29.993194350602646 38.0 25.0 41.0 2.0 41.0 40-41 29.797677687931824 38.0 25.0 41.0 2.0 41.0 42-43 29.571633682788082 38.0 25.0 41.0 2.0 41.0 44-45 29.541520455090343 38.0 25.0 41.0 2.0 41.0 46-47 29.399571718140407 38.0 25.0 41.0 2.0 41.0 48-49 29.267853250800982 38.0 25.0 41.0 2.0 41.0 50-51 29.179306248774004 38.0 25.0 41.0 2.0 41.0 52-53 29.060057540158233 38.0 25.0 41.0 2.0 41.0 54-55 29.024784224406616 37.5 25.0 41.0 2.0 41.0 56-57 28.950657679649527 37.0 25.0 41.0 2.0 41.0 58-59 28.78764902683028 37.0 25.0 41.0 2.0 41.0 60-61 28.621997667879953 36.5 25.0 41.0 2.0 41.0 62-63 28.40223621978597 36.0 24.5 40.5 2.0 41.0 64-65 28.18571140559273 35.5 23.5 40.0 2.0 41.0 66-67 27.914384494670998 35.0 21.5 40.0 2.0 41.0 68-69 27.651258146073538 35.0 18.5 39.5 2.0 41.0 70-71 27.487323183888755 35.0 10.0 39.0 2.0 41.0 72-73 27.250324208277938 35.0 2.0 39.0 2.0 41.0 74-75 26.943064667291473 35.0 2.0 38.5 2.0 41.0 76-77 26.63529020727534 35.0 2.0 37.0 2.0 40.0 78-79 26.320067130184608 35.0 2.0 37.0 2.0 39.0 80-81 26.00700453346701 35.0 2.0 36.5 2.0 39.0 82-83 25.716832675835313 35.0 2.0 36.0 2.0 38.5 84-85 25.400726880408012 35.0 2.0 36.0 2.0 37.0 86-87 25.166027331575165 34.0 2.0 35.0 2.0 37.0 88-89 24.9380898411107 34.0 2.0 35.0 2.0 36.5 90-91 24.752631808373835 34.0 2.0 35.0 2.0 36.0 92-93 24.588784028246987 34.0 2.0 35.0 2.0 36.0 94-95 24.434501754538914 34.0 2.0 35.0 2.0 36.0 96-97 24.255497918528366 34.0 2.0 35.0 2.0 35.5 98-99 24.168999694862798 34.0 2.0 35.0 2.0 35.0 100-101 24.090865499880124 34.0 2.0 35.0 2.0 35.0 102-103 23.523465595780387 32.0 2.0 35.0 2.0 35.0 104-105 23.890499335236807 33.5 2.0 35.0 2.0 35.0 106-107 23.88332316209324 34.0 2.0 35.0 2.0 35.0 108-109 23.82401211830605 34.0 2.0 35.0 2.0 35.0 110-111 23.72533292648373 34.0 2.0 35.0 2.0 35.0 112-113 23.622874937337897 33.5 2.0 35.0 2.0 35.0 114-115 23.49173677557159 33.0 2.0 35.0 2.0 35.0 116-117 23.404511126610146 33.0 2.0 35.0 2.0 35.0 118-119 23.262104683856062 33.0 2.0 35.0 2.0 35.0 120-121 23.080815043264096 33.0 2.0 35.0 2.0 35.0 122-123 22.879026721300754 33.0 2.0 35.0 2.0 35.0 124-125 21.562773806150695 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0989343754502414 1101 2 0.9720559003496696 1101 3 0.6749794097498523 1101 4 1.1326853307390579 1101 5 0.5478052033527021 1101 6 0.930096015674021 1101 7 0.6962389538834373 1101 8 0.6188410971039424 1101 9 0.9668231223446071 1101 10-11 1.0008802264633445 1101 12-13 1.1175083530738412 1101 14-15 1.0169508904118771 1101 16-17 1.146957252118547 1101 18-19 1.0167554305868123 1101 20-21 0.8074646576130746 1101 22-23 0.7336749700754339 1101 24-25 0.4517830167146144 1101 26-27 0.7791648002144527 1101 28-29 0.517521515801274 1101 30-31 0.5379417929359498 1101 32-33 0.23046524584312778 1101 34-35 0.2267752783052721 1101 36-37 0.21391968650200255 1101 38-39 0.45685777171464537 1101 40-41 0.2639006150774783 1101 42-43 0.595369607377549 1101 44-45 0.5917159671822212 1101 46-47 0.44435719142295227 1101 48-49 0.49280999173216955 1101 50-51 0.70896276344574 1101 52-53 0.5793327735761835 1101 54-55 0.7142542298640109 1101 56-57 0.5789224155365993 1101 58-59 0.704417651510493 1101 60-61 0.5842996149312754 1101 62-63 0.7797869148358778 1101 64-65 0.8875187443114925 1101 66-67 0.901714828435825 1101 68-69 0.9476482026262154 1101 70-71 0.8938764660460912 1101 72-73 0.9781636323306486 1101 74-75 0.8480545316707975 1101 76-77 0.8808890455802469 1101 78-79 0.6408210008491722 1101 80-81 0.5662898399554379 1101 82-83 0.6716862340714158 1101 84-85 0.7714843710139832 1101 86-87 0.7306360650305272 1101 88-89 0.5208262176171488 1101 90-91 0.3177048652293273 1101 92-93 0.30695785751271387 1101 94-95 0.15371178371302108 1101 96-97 0.18790374621856287 1101 98-99 0.12500259567914895 1101 100-101 0.12616538017979195 1101 102-103 0.23454582198418095 1101 104-105 -0.00749055057752912 1101 106-107 -0.002678504446848251 1101 108-109 -0.03607965833377946 1101 110-111 -0.027499868969648844 1101 112-113 0.08773694478289684 1101 114-115 0.1421254673099419 1101 116-117 0.03551582336369208 1101 118-119 0.3023730506729301 1101 120-121 0.3327107253309549 1101 122-123 0.33268993611416064 1101 124-125 0.637674679531326 1102 1 0.4434072582338864 1102 2 0.35992604873300493 1102 3 0.4152761764186792 1102 4 0.5811075428818668 1102 5 0.34191522732730206 1102 6 0.1081722160239238 1102 7 0.4798357918281013 1102 8 0.464253525018151 1102 9 0.0265490343513477 1102 10-11 0.2605591612135534 1102 12-13 -0.11590381565550345 1102 14-15 -0.0351614662264943 1102 16-17 0.20521714974052685 1102 18-19 0.03727963197345474 1102 20-21 0.2731874594014485 1102 22-23 0.12192579622718114 1102 24-25 0.1604997276586957 1102 26-27 0.24269263018918252 1102 28-29 0.5865942819517329 1102 30-31 0.6987660008022125 1102 32-33 0.5786639110410796 1102 34-35 0.631516741403825 1102 36-37 0.5984204316584858 1102 38-39 0.3408401471439184 1102 40-41 0.5697306546031733 1102 42-43 0.6354460669123121 1102 44-45 0.620444989407325 1102 46-47 0.6893354846906306 1102 48-49 0.37531015372270815 1102 50-51 0.25112080141632376 1102 52-53 0.7089900015940671 1102 54-55 0.4745082310303417 1102 56-57 0.15074092133585992 1102 58-59 0.09924529357619605 1102 60-61 0.157827120924928 1102 62-63 0.16585896822795831 1102 64-65 -0.021912344912895065 1102 66-67 -0.09771010514805667 1102 68-69 -0.1826083903887472 1102 70-71 -0.25068389162901994 1102 72-73 -0.29421536073615684 1102 74-75 -0.36305153973463433 1102 76-77 -0.30837260153956336 1102 78-79 -0.22462965683241976 1102 80-81 -0.2650375105272964 1102 82-83 -0.3590514708466159 1102 84-85 -0.5027332146790116 1102 86-87 -0.45527237791640474 1102 88-89 -0.08074995033464205 1102 90-91 -0.30735183145958445 1102 92-93 -0.3924929945575215 1102 94-95 -0.5784287592792658 1102 96-97 -0.6080853680171678 1102 98-99 -0.2783862464124738 1102 100-101 -0.3852711519267302 1102 102-103 -0.44662439767498796 1102 104-105 -0.4097697574718424 1102 106-107 -0.4088373847682405 1102 108-109 -0.2616028877771761 1102 110-111 -0.2621836634360548 1102 112-113 -0.01789708618969499 1102 114-115 0.08281263117957138 1102 116-117 -0.05295530992212605 1102 118-119 -0.07717888349411339 1102 120-121 0.2976721715824979 1102 122-123 0.18475207568521412 1102 124-125 -0.1901860734007066 1103 1 0.6276879533841466 1103 2 0.5778976906176894 1103 3 0.5252266124560698 1103 4 0.34150656415045333 1103 5 -0.124911424281958 1103 6 -0.13144444750578543 1103 7 0.7676675253120102 1103 8 0.356546015136729 1103 9 0.3322928074864251 1103 10-11 -0.32227225857439024 1103 12-13 -0.04317340596857022 1103 14-15 0.23653845221255665 1103 16-17 0.026771199555440717 1103 18-19 0.4290309965509067 1103 20-21 0.5482596094736039 1103 22-23 0.22720216237311064 1103 24-25 0.6345178592855234 1103 26-27 0.5038915761707372 1103 28-29 0.6675670531418945 1103 30-31 0.9041270648589297 1103 32-33 1.008795977042709 1103 34-35 0.590618629853541 1103 36-37 0.6139326971707497 1103 38-39 0.5922988333852146 1103 40-41 0.7797736309504941 1103 42-43 0.7239475412833514 1103 44-45 0.5699556237005652 1103 46-47 0.6861279434396081 1103 48-49 0.7036478787995613 1103 50-51 0.5012149102060874 1103 52-53 0.3749351675392312 1103 54-55 0.7029024213810544 1103 56-57 0.6890902531634531 1103 58-59 0.8292550181511373 1103 60-61 0.5897315691337255 1103 62-63 0.6148441617783718 1103 64-65 0.7866860615115989 1103 66-67 0.6242297907048844 1103 68-69 0.4883715958086263 1103 70-71 0.6059178399727116 1103 72-73 0.41057320818284637 1103 74-75 0.18942195295625197 1103 76-77 0.16767671146627094 1103 78-79 0.5302953698317374 1103 80-81 0.3058813049567384 1103 82-83 0.2957104339152892 1103 84-85 0.39625668431088457 1103 86-87 0.3358923752048675 1103 88-89 0.3102485439247218 1103 90-91 0.21690470910130344 1103 92-93 0.4402521588398045 1103 94-95 0.5089355672154916 1103 96-97 0.38319388413165 1103 98-99 0.5352940346591133 1103 100-101 0.48919556493129335 1103 102-103 0.4353004407715879 1103 104-105 0.6998669825996799 1103 106-107 0.3614901138201283 1103 108-109 0.5587355901699951 1103 110-111 0.4923414008716236 1103 112-113 0.2551517249025963 1103 114-115 0.40334716910541246 1103 116-117 0.4532558702020957 1103 118-119 0.6446512187707718 1103 120-121 0.7445634445607254 1103 122-123 0.8347065897701604 1103 124-125 1.0282874820293735 1104 1 0.5994676194017288 1104 2 0.4570945986250088 1104 3 0.5702198265942648 1104 4 -0.15468745248965732 1104 5 0.11234295702446673 1104 6 0.391199678298193 1104 7 -0.1690026818042938 1104 8 0.3574753831664701 1104 9 0.7374825165794796 1104 10-11 1.0002479514922875 1104 12-13 0.22331918859093847 1104 14-15 0.5169051837874648 1104 16-17 0.5992471540016595 1104 18-19 1.1709543458162628 1104 20-21 1.0813996932642453 1104 22-23 0.8339172151848189 1104 24-25 0.7636926394826062 1104 26-27 0.8257627038039459 1104 28-29 0.9193741576441674 1104 30-31 0.9347667060933631 1104 32-33 0.811777330674623 1104 34-35 1.002896614157546 1104 36-37 1.0740878868798447 1104 38-39 0.5866341139658466 1104 40-41 0.8210567489064751 1104 42-43 0.9929258265255747 1104 44-45 0.9924640322684546 1104 46-47 1.0738440583862072 1104 48-49 1.24386886186441 1104 50-51 1.3110485090731387 1104 52-53 1.2067933135237716 1104 54-55 1.370845125104431 1104 56-57 1.4030661781059202 1104 58-59 1.0605865222521587 1104 60-61 1.1840771760257987 1104 62-63 1.043895026238161 1104 64-65 1.1276461134381925 1104 66-67 1.1778971064539832 1104 68-69 1.1467919651955398 1104 70-71 1.4358656183405643 1104 72-73 1.4726712196302998 1104 74-75 1.435933134876354 1104 76-77 1.1082414131833893 1104 78-79 1.536122359859469 1104 80-81 1.7370269354518122 1104 82-83 1.5870451834284793 1104 84-85 1.6190984852196024 1104 86-87 1.6563399470464937 1104 88-89 1.5065886041123662 1104 90-91 1.5093679769363177 1104 92-93 1.5159513976431285 1104 94-95 1.4670992540037169 1104 96-97 1.4073869566518589 1104 98-99 1.3544803213175172 1104 100-101 1.3067370176957915 1104 102-103 1.1930746780997303 1104 104-105 1.402292190601095 1104 106-107 1.1786701456841726 1104 108-109 1.0168181416540314 1104 110-111 0.9896934395805346 1104 112-113 1.0046575371407478 1104 114-115 0.971677847101521 1104 116-117 0.9617529493311459 1104 118-119 0.8636427806991378 1104 120-121 0.9431531654552785 1104 122-123 1.1304152697725236 1104 124-125 1.2656303745767268 1105 1 0.004672080368273157 1105 2 0.10277040699984141 1105 3 -0.2922337050042465 1105 4 -0.7073703451038114 1105 5 -0.3611647935541953 1105 6 -0.142390838640857 1105 7 -0.14055349894675118 1105 8 -0.453831343353837 1105 9 0.024401223179744136 1105 10-11 0.005775060982365687 1105 12-13 0.056032943237777744 1105 14-15 -0.32052204494227965 1105 16-17 -0.43017698852482056 1105 18-19 -0.893498203808484 1105 20-21 -1.0242396417992339 1105 22-23 -0.5114920574531823 1105 24-25 -0.17320151777347803 1105 26-27 0.3752752239694743 1105 28-29 0.4607012250496503 1105 30-31 0.3779665377550039 1105 32-33 0.5117983729696363 1105 34-35 0.28501066339651615 1105 36-37 -0.26916104347015946 1105 38-39 0.12042102565834867 1105 40-41 0.14267419998313713 1105 42-43 0.1387349027688245 1105 44-45 0.19659533618933622 1105 46-47 0.26933399315509376 1105 48-49 0.20401475510983502 1105 50-51 -0.15372668907513543 1105 52-53 -0.0676823826254882 1105 54-55 -0.09592207697174615 1105 56-57 -0.15857371808542098 1105 58-59 -0.21603548798842453 1105 60-61 -0.2783202427860161 1105 62-63 -0.30482490081521973 1105 64-65 -0.4472013299229616 1105 66-67 -0.6454317846170703 1105 68-69 -0.728055478165615 1105 70-71 -0.559603274133309 1105 72-73 -0.5084818981364094 1105 74-75 -0.6241075468940025 1105 76-77 -0.43129004504765334 1105 78-79 -0.3676231686528375 1105 80-81 -0.3724280691073538 1105 82-83 -0.49490052745039037 1105 84-85 -0.4670556846618581 1105 86-87 -0.3476580890059715 1105 88-89 -0.6255780508641386 1105 90-91 -0.633207599973268 1105 92-93 -0.56691316011797 1105 94-95 -0.6187673378458989 1105 96-97 -0.43875879631159975 1105 98-99 -0.39751518965954347 1105 100-101 -0.6112611586386407 1105 102-103 -0.5407402899861218 1105 104-105 -0.5797376894578221 1105 106-107 -0.6672160856407885 1105 108-109 -0.6623710285781321 1105 110-111 -0.6345753406677623 1105 112-113 -0.6828012550315208 1105 114-115 -0.556041869528908 1105 116-117 -0.8502481729245872 1105 118-119 -0.5670438995281195 1105 120-121 -0.44911729817662405 1105 122-123 -0.6509552850426594 1105 124-125 -0.7926918531009086 1106 1 0.6528934242417925 1106 2 0.2255386969543487 1106 3 0.3125093779997066 1106 4 0.9447439065182301 1106 5 0.7253144368134699 1106 6 0.9224014650357795 1106 7 0.6577014044763239 1106 8 0.5551626159272445 1106 9 0.9830707734817814 1106 10-11 0.6736026394744243 1106 12-13 0.8050448009452857 1106 14-15 1.180253553536346 1106 16-17 1.23486141851523 1106 18-19 0.8060543355307672 1106 20-21 1.0241756016939476 1106 22-23 0.8926767843694776 1106 24-25 1.0873771980144227 1106 26-27 0.8889376894777143 1106 28-29 0.9897563372876981 1106 30-31 0.8845367517903533 1106 32-33 0.7762923695391066 1106 34-35 0.9615562670955242 1106 36-37 1.1478275462829899 1106 38-39 1.2005239416103208 1106 40-41 1.117161484642697 1106 42-43 0.9852484384538123 1106 44-45 1.0058204834784696 1106 46-47 1.1379520854811425 1106 48-49 1.2468516556989933 1106 50-51 1.2135713943017024 1106 52-53 1.3236145075229189 1106 54-55 1.371741432611369 1106 56-57 1.6546934905058208 1106 58-59 1.530075333101891 1106 60-61 1.2269970813992366 1106 62-63 1.2903648970817159 1106 64-65 1.312079749948765 1106 66-67 1.3252147960377165 1106 68-69 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-1.4035480929921995 2216 104-105 -1.372974018207426 2216 106-107 -1.373174938641533 2216 108-109 -1.3837919858321577 2216 110-111 -1.3036298124724723 2216 112-113 -1.1920166904861595 2216 114-115 -1.2074276492965232 2216 116-117 -1.2848393678931416 2216 118-119 -1.5433364056575307 2216 120-121 -1.691809974863542 2216 122-123 -1.6715820838913942 2216 124-125 -1.6282904033620724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11506.0 3 2927.0 4 4317.0 5 4314.0 6 5583.0 7 3615.0 8 2757.0 9 1933.0 10 1742.0 11 1590.0 12 1207.0 13 1038.0 14 1105.0 15 1089.0 16 945.0 17 903.0 18 812.0 19 743.0 20 688.0 21 731.0 22 853.0 23 2952.0 24 5865.0 25 1999.0 26 5920.0 27 3043.0 28 1953.0 29 1843.0 30 1960.0 31 2073.0 32 2643.0 33 3412.0 34 4718.0 35 9217.0 36 11429.0 37 40078.0 38 33895.0 39 126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.671956922204906 27.595077446289007 12.176126268516803 15.556839362989287 2 11.651607137018104 38.576193227026494 32.37272063391718 17.399479002038216 3 12.405656521620664 50.21661535108035 22.209204108879867 15.168524018419117 4 7.437205651491366 33.907858015000876 31.438055991627422 27.21688034188034 5 7.002540035538694 56.92950038700112 25.91490335873369 10.153056218726494 6 17.290282493881932 53.95289770156917 15.362478402816762 13.394341401732134 7 18.64155549742785 52.410846630046215 18.103692562559946 10.843905309965995 8 18.392144896580284 51.490268302783285 16.316122142063165 13.801464658573265 9 20.65960774483251 37.48058614845534 15.124548372506172 26.73525773420598 10-11 15.490344587083978 48.22829711645343 21.482203962424535 14.799154334038056 12-13 17.12466487935657 48.04649185902047 20.46797009525055 14.360873166372414 14-15 16.554488576909662 47.89986266131845 19.444323334495987 16.1013254272759 16-17 14.033118464715594 49.76978362385096 20.88484822065899 15.312249690774454 18-19 13.88381787962181 48.75807198713932 22.91844908858069 14.439661044658184 20-21 16.928312209156594 47.52939766126483 21.967327455617433 13.574962673961139 22-23 16.62507355986138 47.711198535341424 22.048614895054598 13.615113009742593 24-25 14.976853680932283 48.188756256590366 22.760501671602913 14.073888390874437 26-27 14.359702273272159 48.730138837427255 22.643359996512718 14.266798892787863 28-29 14.883252050241126 47.22257036209574 23.778982644470478 14.115194943192655 30-31 14.701427069086709 48.981871482048575 23.1714825934624 13.145218855402321 32-33 14.334560255882826 49.273245913966484 22.698157981489906 13.694035848660786 34-35 14.330317370799925 48.51340564014963 23.307369355968035 13.84890763308241 36-37 14.146954166734782 49.37960798417551 22.125408009241852 14.34802983984786 38-39 13.393961519809505 50.19016690006157 23.011557134528097 13.404314445600823 40-41 13.087514407710271 49.932014681558954 23.73000972775899 13.25046118297179 42-43 12.942381729556235 50.12465294712263 22.666129001495836 14.266836321825302 44-45 13.302656063119983 49.718700874819845 22.86546409587873 14.113178966181442 46-47 12.76736620109753 49.57548377955674 23.337765595112884 14.319384424232846 48-49 12.674333385098668 50.27694453753123 22.406488648407127 14.642233428962978 50-51 13.63061698255098 49.793467243577865 21.734439200898063 14.841476572973091 52-53 12.519787372591118 49.8516457917265 22.54411800766701 15.084448828015379 54-55 13.115334735557274 49.753975086912455 22.48743992414913 14.64325025338114 56-57 13.346721016664667 49.707632614358424 21.549901363472877 15.395745005504027 58-59 12.68094220749493 48.770737502997534 22.731409823199844 15.816910466307688 60-61 13.503086840198126 49.77168825366034 21.039281608099344 15.685943298042185 62-63 12.943885127310873 49.59960764546408 22.111085377981826 15.345421849243218 64-65 13.560374891020052 47.99885571054926 22.56102877070619 15.879740627724498 66-67 13.305561761324316 48.76744350782744 21.522060145705396 16.404934585142843 68-69 13.326049998910225 48.198873171901226 22.375820056232428 16.099256772956124 70-71 13.68026701178394 46.76793133982699 23.544990123288603 16.00681152510047 72-73 13.209788418890275 47.36463549527852 22.60670324700446 16.81887283882674 74-75 13.566539882515775 47.41927046437874 23.167714724762142 15.84647492834334 76-77 13.612037582019926 46.2693196433632 24.21304471039609 15.905598064220783 78-79 13.392236834577066 46.106838200636986 24.63730209976542 15.86362286502053 80-81 14.024624488806184 46.86908261654947 23.455384120773882 15.65090877387047 82-83 14.54344835101963 45.598223651050965 23.831410317535997 16.026917680393414 84-85 14.236012728580457 45.021632048124495 24.07750484950197 16.664850373793076 86-87 14.540882353742498 45.891603390356934 23.873627196961646 15.693887058938918 88-89 14.868892836513837 44.533114639755006 24.597305927613153 16.00068659611801 90-91 14.490956945360223 44.4191356376457 24.840200314967493 16.24970710202658 92-93 14.964440447968611 45.945393607455244 23.228425842666013 15.861740101910135 94-95 14.36787559120333 44.87478476929448 23.923301584533903 16.834038054968286 96-97 14.656746020934705 45.525901385657384 23.49542019256442 16.32193240084349 98-99 13.74878758486906 46.33278479495199 23.55681731492279 16.36161030525616 100-101 13.098831760423705 46.82630064732678 23.712157537978683 16.36271005427083 102-103 13.839061921056647 47.76950153658377 22.81036812623962 15.581068416119962 104-105 12.693352515319702 49.49688156485773 22.777246458553012 15.032519461269553 106-107 12.52996883241429 49.38618382336915 22.76677709727338 15.31707024694318 108-109 12.710871602624179 50.49312351518057 21.3617837449053 15.434221137289947 110-111 12.67221908910848 51.2272270308707 21.11555195928587 14.985001920734947 112-113 12.317245287748419 51.61897651911874 22.016936314444365 14.04684187868847 114-115 12.263174241639922 51.721873791010296 21.373575776069224 14.64137619128056 116-117 12.158355310477104 51.817201020030076 21.33235980035309 14.69208386913973 118-119 12.328110227424439 50.57475961996932 21.71008356424529 15.387046588360947 120-121 12.17551220575414 50.91488666085441 21.534710113339145 15.374891020052312 122-123 12.302205706065692 50.8538392798762 21.68517469104858 15.158780323009523 124-125 12.629409182496598 51.152106401136436 21.070555099614737 15.147929316752235 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8511.0 1 8120.0 2 9320.5 3 8339.0 4 4297.5 5 2158.0 6 1285.0 7 1116.5 8 1240.5 9 1233.5 10 847.5 11 506.0 12 434.5 13 396.5 14 386.0 15 374.5 16 358.0 17 362.5 18 387.0 19 436.5 20 477.0 21 498.5 22 561.5 23 666.0 24 696.0 25 774.5 26 936.5 27 1092.5 28 1335.0 29 1909.0 30 2748.5 31 3255.0 32 4495.5 33 6206.0 34 7180.5 35 7842.0 36 9601.5 37 11003.5 38 10126.5 39 9227.5 40 8670.5 41 7769.0 42 5866.0 43 4970.5 44 5385.5 45 4864.5 46 3609.0 47 2594.5 48 2242.0 49 1899.5 50 1582.0 51 1080.0 52 739.0 53 649.5 54 578.0 55 571.5 56 557.5 57 498.0 58 387.0 59 367.0 60 382.0 61 361.5 62 288.0 63 167.0 64 106.5 65 124.0 66 126.0 67 96.5 68 78.5 69 62.0 70 48.0 71 36.0 72 26.0 73 48.5 74 53.0 75 26.5 76 18.5 77 14.5 78 12.0 79 10.5 80 6.0 81 5.0 82 4.0 83 4.0 84 4.0 85 3.0 86 2.0 87 3.5 88 3.0 89 1.5 90 3.0 91 2.5 92 1.0 93 1.0 94 0.5 95 1.5 96 2.0 97 3.0 98 3.0 99 3.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022885290207275342 2 0.01634663586233953 3 0.010352869379481702 4 0.03705237462130293 5 0.03378304744883503 6 0.0277892809659772 7 0.010897757241559686 8 0.0 9 0.011442645103637671 10-11 0.0 12-13 0.004359102896623874 14-15 0.019615963034807435 16-17 5.448878620779843E-4 18-19 0.010352869379481702 20-21 0.003269327172467906 22-23 0.0 24-25 0.009535537586364726 26-27 0.0 28-29 0.004903990758701859 30-31 5.448878620779843E-4 32-33 0.0013622196551949608 34-35 2.7244393103899214E-4 36-37 0.005993766482857828 38-39 5.448878620779843E-4 40-41 0.015529304069222552 42-43 0.007900874000130774 44-45 2.7244393103899214E-4 46-47 0.011442645103637671 48-49 0.001907107517272945 50-51 0.009807981517403717 52-53 0.0057213225518188355 54-55 0.003269327172467906 56-57 0.011987532965715655 58-59 0.021795514483119373 60-61 0.001634663586233953 62-63 0.008990649724286742 64-65 0.002179551448311937 66-67 0.001634663586233953 68-69 0.0 70-71 0.006266210413896821 72-73 5.448878620779843E-4 74-75 0.005448878620779843 76-77 0.017436411586495497 78-79 0.001907107517272945 80-81 0.004086658965584883 82-83 8.173317931169765E-4 84-85 0.0 86-87 0.001907107517272945 88-89 0.005448878620779843 90-91 0.007083542207013797 92-93 0.01580174800026155 94-95 0.0 96-97 5.448878620779843E-4 98-99 0.003269327172467906 100-101 0.0 102-103 0.0 104-105 0.01008042544844271 106-107 0.0 108-109 0.0 110-111 2.7244393103899214E-4 112-113 0.007083542207013797 114-115 0.001634663586233953 116-117 0.0 118-119 0.0068110982759748044 120-121 0.002179551448311937 122-123 0.0 124-125 0.07764652034611276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 183524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.931365924892656 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24137411481091 22.92561190906911 2 7.245366882627693 4.192367210828012 3 3.211164682838632 2.7871014145288897 4 1.7609612776857013 2.0378806041716615 5 1.2618653005876148 1.8253743379612477 6 0.9096730450504745 1.5790850243019987 7 0.7156847973481995 1.4494017131274384 8 0.5744312189242128 1.329526383470282 9 0.4463613078197981 1.1622458098123405 >10 3.847747476269399 23.30812318824786 >50 0.4953292150067802 9.704997711470979 >100 0.26932348952840135 13.440204006015563 >500 0.01130028627391894 2.57078093328393 >1k 0.007533524182612626 6.147424859963819 >5k 0.0 0.0 >10k+ 0.0018833810456531565 5.539874893746867 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10167 5.539874893746867 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4342 2.365903097142608 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3846 2.0956387175519278 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1991 1.0848717333972668 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1103 0.6010113118720167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.5388940955951266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 0.44844271049018114 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 0.42283298097251587 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 755 0.4113903358688782 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 711 0.38741526993744685 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 664 0.36180554041978163 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 491 0.2675399402802903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.26045639807327653 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 464 0.2528279680041848 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 391 0.21305115407249187 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 388 0.21141649048625794 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 380 0.20705738758963407 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.19615963034807435 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.18744142455482662 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 327 0.17817833089950086 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 315 0.17163967655456508 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 310 0.16891523724417515 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 292 0.15910725572677145 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 290 0.15801748000261545 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 281 0.1531134892439136 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 280 0.15256860138183562 No Hit GAACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 278 0.15147882565767964 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 270 0.14711972276105578 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 268 0.1460299470368998 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 264 0.14385039558858786 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 254 0.13840151696780803 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 252 0.13731174124365206 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 247 0.13458730193326213 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 243 0.13240775048495018 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 236 0.1285935354504043 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 233 0.12695887186417035 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 232 0.1264139840020924 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 230 0.1253242082779364 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 215 0.11715089034676662 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 213 0.11606111462261068 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 212 0.11551622676053268 No Hit GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT 210 0.11442645103637672 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 203 0.11061223600183083 No Hit GAACAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 199 0.10843268455351887 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 199 0.10843268455351887 No Hit GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.1078877966914409 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.1078877966914409 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 192 0.104618469518973 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 192 0.104618469518973 No Hit ATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTC 188 0.10243891807066106 No Hit GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT 188 0.10243891807066106 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 187 0.10189403020858308 No Hit GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA 185 0.1008042544844271 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.448878620779844E-4 0.0 5 0.0 0.0 0.0 5.448878620779844E-4 0.0 6 0.0 0.0 0.0 5.448878620779844E-4 0.0 7 0.0 0.0 0.0 5.448878620779844E-4 0.0 8 0.0 0.0 0.0 5.448878620779844E-4 0.0 9 0.0 0.0 0.0 5.448878620779844E-4 0.0 10-11 0.0 0.0 0.0 8.173317931169765E-4 0.0 12-13 0.0 0.0 0.0 0.0019071075172729453 0.0 14-15 0.0 0.0 0.0 0.0027244393103899216 0.0 16-17 0.0 0.0 0.0 0.00626621041389682 0.0 18-19 0.0 0.0 0.0 0.007628430069091781 0.0 20-21 0.0 0.0 0.0 0.010897757241559686 0.0 22-23 0.0 0.0 0.0 0.020433294827924414 0.0 24-25 0.0 0.0 0.0 0.04004925786273185 0.0 26-27 0.0 0.0 0.0 0.06756609489767006 0.0 28-29 0.0 0.0 0.0 0.09916959089819316 0.0 30-31 0.0 0.0 0.0 0.19534229855495738 0.0 32-33 0.0 0.0 0.0 0.28742834724613675 0.0 34-35 0.0 0.0 0.0 0.37243085373030227 0.0 36-37 0.0 0.0 0.0 0.47377999607680743 0.0 38-39 0.0 0.0 0.0 0.6396983500795537 0.0 40-41 0.0 0.0 0.0 0.8519321723589286 0.0 42-43 0.0 0.0 0.0 1.0208474096031037 0.0 44-45 0.0 0.0 0.0 1.1693293520193544 0.0 46-47 0.0 0.0 0.0 1.3360650378152177 0.0 48-49 0.0 0.0 0.0 1.5491161918877094 0.0 50-51 0.0 0.0 0.0 1.7657091170637083 0.0 52-53 0.0 0.0 0.0 1.9512434341012619 0.0 54-55 0.0 0.0 0.0 2.1283319892766066 0.0 56-57 0.0 0.0 0.0 2.3174080774176673 0.0 58-59 0.0 0.0 0.0 2.516019703145093 0.0 60-61 0.0 0.0 0.0 2.7413308341143394 0.0 62-63 0.0 0.0 0.0 2.9748152830147556 0.0 64-65 0.0 0.0 0.0 3.1971295307425733 0.0 66-67 0.0 0.0 0.0 3.4355179704016914 0.0 68-69 0.0 0.0 0.0 3.692977485233539 0.0 70-71 0.0 0.0 0.0 4.008740001307731 0.0 72-73 0.0 0.0 0.0 4.334582942830366 0.0 74-75 0.0 0.0 0.0 4.624463285455853 0.0 76-77 0.0 0.0 0.0 4.9167956234606915 0.0 78-79 0.0 0.0 0.0 5.282960266777097 0.0 80-81 0.0 0.0 0.0 5.691353719404546 0.0 82-83 0.0 0.0 0.0 6.087214751204202 0.0 84-85 0.0 0.0 0.0 6.484710446590092 0.0 86-87 0.0 0.0 0.0 6.909178091148842 0.0 88-89 0.0 0.0 0.0 7.319206207362525 0.0 90-91 0.0 0.0 0.0 7.78835465661167 0.0 92-93 0.0 0.0 0.0 8.318802990344587 0.0 94-95 0.0 0.0 0.0 8.822279374904644 0.0 96-97 0.0 0.0 0.0 9.355724591878992 0.0 98-99 0.0 0.0 0.0 9.871188509404764 0.0 100-101 0.0 0.0 0.0 10.449314531069506 0.0 102-103 0.0 0.0 0.0 11.128517251149713 0.0 104-105 0.0 0.0 0.0 11.816165733092129 0.0 106-107 0.0 0.0 0.0 12.486922691310127 0.0 108-109 0.0 0.0 0.0 13.117085503803317 0.0 110-111 0.0 0.0 0.0 13.81372463547002 0.0 112-113 0.0 0.0 0.0 14.518537085067894 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACTA 15 2.6919984E-4 119.0671 2 TCAGGCC 25 1.7170189E-5 95.253685 3 GTCAGGC 25 1.7170189E-5 95.253685 2 GAACAAA 900 0.0 93.269226 1 TAAAGGT 20 8.451189E-4 89.30033 5 TCACTAC 20 8.451189E-4 89.30033 3 CGTCACT 20 8.451189E-4 89.30033 1 GTACATA 35 8.8983506E-7 85.047935 1 TCATGGG 35 8.8983506E-7 85.047935 2 GAAAACA 535 0.0 81.2327 1 TATGGTG 30 4.2427673E-5 79.37807 2 GTATGGT 30 4.2427673E-5 79.37807 1 GGTATCA 390 0.0 71.74556 1 AAAGGTT 25 0.0020561574 71.38184 6 CGCAGAA 55 1.712724E-7 64.945694 2 GTATCAA 840 0.0 61.659748 1 GTACAAG 180 0.0 59.533558 1 TAACCCG 20 1.7726117E-4 59.484875 80-81 GGACAAC 30 0.0042351484 59.48487 6 ATGGGGA 115 0.0 56.945137 5 >>END_MODULE